• Journal of the Korean Society of Tobacco Science
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• v.32 no.1
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• pp.1-11
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• 2010

This study was carried out to investigate the influence of stem materials such as black stem on the quality of expended stem and cigarettes. Normal and black stem were separated by tobacco scan and then, those stems were expanded after treating with their respective stem casings. Total sugar, ether extract, ash contents and pH were slightly low in black stem compared with normal stem. However, the number of bacteria and fungi ratio were remarkably higher in black stem than that of normal stem. As compared with normal stems, ratio of rushed stem in rolled process was approximately 2 times higher in black stem with the consequency that the filling capacity of black stem was decreased. The ratio of large particles (> 3.35 mm) of expanded black stem showed decreasing tendency and small particles rate (1.40 mm <) was increased compared with normal stem. When expanded stems were prepared using stem containing 5 levels (0, 10, 20, 30 and 100 %) of black stem, the filling capacity was decreased and static burning rate was significantly decreased with increasing expanded black stem rate. However, the weight and hardness of cigarettes were slightly increased with increasing expanded black stem rate. The contents of phenol compounds, aromatic amines and carbonyl compounds in the cigarette mainstream smoke from the cigarette which was manufactured with various ratio of expended black stem, were gradually increased with increasing expanded black stem rates. Also, the cytotoxicity and the mutagenicity of the TPM were significantly increased with increasing expanded black stem rate. The sensory test result showed that cigarettes blended with 10 and 30 % level of black stem rate was exhibited significantly high sensory attributions such as off-taste, impact, hotness, bitterness and irritation as compared with cigarette blended with normal stem, while smoke fullness and cleanness were slightly decreased with increasing expanded black stem rates. The number of brown spots on cigarettes paper was 2 to 3 times high in cigarettes containing black stem than that of cigarette made from normal stem and were high with increasing black stem rate. The overall assessment in this study suggest, that black stem should not be used because of bad quality of expanded stem and high toxicological activity of cigarette mainstream smoke.

• IMMUNE NETWORK
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• v.3 no.2
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• pp.83-88
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• 2003

The number of umbilical cord blood transplantation is increasing worldwide as it has expanded the ability of the transplantaion community to meet the growing needs of their patients. Clinical data over the last decade show promising results in transplantation using both related as well as unrelated cord bloods. Cord blood banks are essential for the clinical use for transplantation and are now established around the world with the major efforts to standardize banking in collection, processing and distribution of cord blood for providing the highest quality stem cells for the patients. In Korea, Medipost, Histostem and some regional cord blood banks were established some years ago and collected thousands of cord blood for public but it had some limitations and was not expanded as the cord blood transplantation was not covered by medical insurance. Recently with the change in the policy of medical insurance to cover the cord blood transplantation, several venture companies are showing great interests in cord blood banking and trying to establish private cord blood banks in Korea. This review article discusses the current status of cord blood transplantaion and also the clincial use of stem cells from cord blood.

• Clinical and Experimental Pediatrics
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• v.50 no.7
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• pp.613-621
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• 2007

Hematopoietic stem cell transplantation (HSCT) has expanded and evolved substantially in the last decades to treat various malignant and nonmalignant diseases. However, the conditioning regimen can lead to transplantation related death by major organ dysfunction, severe infection and bleeding. In the allogeneic setting, graft versus host disease may also develop, making post-transplant management complex. To overcome these problems, new stem cell sources, stem cell mobilizing agents and new skills, nonmyeloablative stem cell transplantation including reduced intensity stem cell transplantation has been introduced in clinical practice, but problems remained so far. Recipients of stem cell transplant may be severely immunocompromised for many months after transplantation. Furthermore, long-term complications (endocrine, metabolic, relapse, second malignancies, etc) can develop. Pediatrician is open called on to participate in the evaluation and consideration of patients for possible transplant and long-term follow-up of HSCT patients. This review is intended as a basic overview of HSCT relevant to general pediatrician.

• Journal of the Korean Society of Tobacco Science
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• v.26 no.1
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• pp.43-49
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• 2004

This study was carried out to investigate moisture sorption and desorption characteristics followed by tobacco type. Experiments were performed at various temperature(5, 15, 25, 40 $^{\circ}C$) and relative humidity range (11～84 %) controlled by saturated salt solution. Regression equation was obtained to predict equilibrium moisture according to various relative humidity, temperature and tobacco types. The obtained regression equation showed high $R^2$(above 0.95) and predicted accurate equilibrium moisture. Equilibrium moisture contents declines in the following order when a relative humidity is 50 % or above: expanded stem, flue-cured, expanded tobacco, reconstituted tobacco, USA flue-cured, orient, burley. To maintain 13 % moisture of each tobacco type in the range of 5～40 $^{\circ}C$ it is recommendable to control relative humidity 49～56 % for expanded stem, 50～57 % for flue-cured, 54～61 % for USA flue-cured, 56～60 % for reconstituted tobacco, 57～62 % for expanded tobacco, 58～64 % for orient and 58～65 % for burley, respectively. It means that the relative humidity of each tobacco type should be differently controlled to maintain the same moisture under the same temperature. In the range of 5～25 $^{\circ}C$, the lower temperature showed the higher equilibrium moisture content.

• Journal of the Korean Society of Tobacco Science
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• v.22 no.1
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• pp.84-90
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• 2000

This study was conducted to determine the acid and phenol compounds in smoke total particulate matter(TPM) by the different tobacco variety, and grade of tobacco leaves. Sixteen kinds of tobacco leaves which were flue-curd, burley, orient, reconstituted tobacco, expanded stem, and expanded cut tobacco, were selected for this study. After collecting a TPM by using smoking machine, the concentration of TPM components was analyzed by GC. Acid components of TPM of mainstream smoke were different from the variety and grade. The order of the highest concentration of acid compounds in TPM was flue-cured > orient> burley> expanded cut tobacco> reconstituted tobacco> expanded stem. Though lactic acid and glycolic acid concentrations in flue-cured tobacco were twice higher than those in burley tobacco, the contents of 2-furoic acid and 3,4-dihydroxy butanoic acid in burley tobacco were higher than those in flue-cured tobacco. The content of phenolic compounds in the high grade and thick leaves was higher than that in other tobacco leaves. Phenol and catechol compounds in burley CD3W-2 revealed the least value in concentration among the samples tested. Pyrocatechol and hydroquinone concentrations in flue-cured tobacco were 2-3 times higher than those in burley and orient tobacco.

• Biomedical Science Letters
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• v.21 no.1
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• pp.40-49
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• 2015

Mesenchymal stem cells (MSCs) have the ability to self-renew and differentiate into multi-lineage cells, thus highlighting the feasibility of using umbilical cord blood-derived MSCs (UCB-MSCs) for cell-therapy and tissueengineering. However, the low numbers of UCB-MSC derived from clinical samples requires that an ex vivo expansion step be implemented. As most stem cells reside in low oxygen tension environments (i.e., hypoxia), we cultured the UCBMSCs under 3% $O_2$ or 21% $O_2$ and the following parameters were examined: proliferation, senescence, differentiation and stem cell specific gene expression. UCB-MSCs cultured under hypoxic conditions expanded to significantly higher levels and showed less senescence compared to UCB-MSCs cultured under normoxic conditions. In regards to differentiation potential, UCB-MSCs cultured under hypoxic and normoxic conditions both underwent similar levels of osteogenesis as determined by ALP and von Kossa assay. Furthermore, UCB-MSCs cultured under hypoxic conditions exhibited higher expression of OCT4, NANOG and SOX2 genes. Moreover, cells expanded under hypoxia maintained a stem cell immnunophenotype as determined by flow cytometry. These results demonstrate that the expansion of human UCB-MSCs under a low oxygen tension microenvironment significantly improved cell proliferation and differentiation. These results demonstrate that hypoxic culture can be rapidly and easily implemented into the clinical-scale expansion process in order to maximize UCB-MSCs yield for application in clinical settings and at the same time reduce culture time while maintaining cell product quality.

• Journal of the Korean Society of Tobacco Science
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• v.28 no.1
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• pp.23-30
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• 2006

In order to improve the final product quality, tobacco industry has gradually decreased the size and number of steams in the cigarette. Especially, steams bigger than $3{\times}3$ mm($length{\times}thickness$) and $2{\times}10$ mm($width{\times}length$) are not acceptable in the process as objectionable stems. A total number of 12 samples where the $length{\times}tickness$ samples were prepared by decreasing the present sample ($3{\times}3$ mm) with 1 mm up to $1{\times}1$ mm, and the $width{\times}length$ samples were prepared by decreasing the present sample ($2{\times}10$ mm) with 2 mm up to $2{\times}6$ mm for the stems in leaf and stems in expanded cut-tobacco were prepared to apply a fire ball drop test using stems. In addition, cigarettes were produced for these stems in order to analyze the forced fire ball drop including the burning, encapsulation and unencapsulation pressure drop and a single or unspecified direction combustion. Based on the results of the test, it was evident that the fire ball drop in the stems in expanded cut-tobacco presented a lower level than that of the stems in leaf. Also, the stems in expanded cut-tobacco presented a larger swelling and higher burning for a single direction than that of the stem in leaf. In addition, it was considered that the management criteria for objectionable stems should be changed as $3{\times}3$ mm and $2{\times}8$ mm for the $length{\times}thickness$ and $width{\times}length$ sample, respectively, in both steams in leaf and expanded cut-tobacco.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.234-234
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• 2005

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.390-391
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• 2005

• Nuclear Engineering and Technology
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• v.55 no.8
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• pp.2905-2918
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• 2023

The valve assembly used in nuclear power plants is important safety-related equipment. In the new standard, the physical attributes are measured using a valve diagnosis test, which is used in the expansion to other non-tested valves using a quantitative test-basis methodology. With a motor-operated actuator, the state of stem's lubrication is related to physical attributes such as the stem factor and the friction coefficient. This study analyzed the numerical transient of fluid and solid lubrication with a squeeze film effect due to the loading rate on the stem and the stem nut using the experimental data. The differential equation that governs the motion mechanism of the stem and stem nut is established and analyzed. The flow rate, the fluid and the solid contact forces are calculated with the friction coefficient. Finally, we found that a change in the friction coefficient results from a change of the shear force in the solid contact mode during the interchange process between the solid contact mode and the fluid contact mode. The qualitative understanding of the squeeze film effect is expanded quantitatively for forces, thread surface distance, velocity, and acceleration, with consideration of the metal solid contact and fluid contact.