• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.216-216
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• 2022

The starch-branching enzymes (BEs) are responsible for synthesizing the amylopectin, which plays an important role in determining the structural and physical properties of starch granules. BE has two differently functioning isoforms (BEI and BEIIa/b) based on their difference in the chain-length pattern by the degree of polymerization (DP), which mainly contributes to the amylopectin chain length distribution in starch biosynthesis. In this study, we investigated functional haplotypes and evolutionary analyses of SBE1 in 374 rice accessions (320 Korean bred and 54 wild). The analyses were performed based on the classified subpopulations. Haplotype analysis generates a total of 8 haplotypes, of which only four haplotypes were functional carrying four functional SNPs in four different exons of SBE1 on chromosome 6. Nucleotide diversity analysis showed a highest pi-value in aromatic group (0.0029), while the lowest diversity value was in temperate japonica (0.0002), indicating the signal of this gene evolution origin. Different directional selections could be estimated by negative Tajima's D value of temperate japonica (-1.1285) and positive Tajima's D value of tropical japonica (0.9456), where the selective sweeps were undergone by both positive purifying and balancing selections. Phylogenetic analysis indicates a closer relationship of the wild with most of the cultivated subgroups indicating a common ancestor for SBE1 gene. FST-values indicate distant genetic relationships of temperate japonica from all other classified groups. PCA and population structure analysis show an admixed structure of wild and cultivated subpopulations in some proportions.

• Reproductive and Developmental Biology
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• 제39권4호
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• pp.105-115
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• 2015

Toll-like receptor 7 (TLR7) is critical for the triggering of innate immune response by recognizing the conserved molecular patterns of single-stranded RNA (ssRNA) viruses and mediated antigenic adaptive immunity. To understand how TLR7 distinguish pathogen-derived molecular patterns from the host self, it is essential to be able to identify TLR7 receptor interaction interfaces, such as active sites or R848-agonist binding sites. The functional interfaces of TLR7 can serve as targets for structure-based drug design in studying the TLR7 receptor's structure-function relationship. In contrast to mammalian TLR7, chicken TLR7 (chTLR7) is unknown for its important biological function. Therefore, it has been targeted to mediate contrasting evolutionary patterns of positive selection into non-synonymous SNPs across eleven species using TLR7 conservation patterns (evolutionary conserved and class-specific trace residues), where protein sequence differences to the TLR7 receptors of interest record mutation that have passed positive section across the species. In this study, we characterized the Lys609 residue on chTLR7-ECD homodimer interfaces to reflect the current tendency of evolving positive selection to be transfer into a stabilization direction of the R848-agonist/chTLR7-ECDs complex under the phylogenetically variable position across species and we suggest a potential indicator for contrasting evolutionary patterns of both the species TLR-ECDs.

• 한국응용곤충학회지
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• 제42권4호
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• pp.367-381
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• 2003

곤충의 분산다형성이란 비상능력과 관련된 다형성으로 그 구체적 내용으로는 시다형성, 비상근다형성 그리고 비상행동변이성 등과 이들과는 별개로 개체군 밀도의존적인 상변이성이 있다. 분산다형성은 시간적으로나 공간적으로 이질적인 서식처 환경에 대응하기 위하여 이동형인 “유시형이나 장시형”과 정주형인 “무시형이나 단시형”을 생활사에 적절히 짜넣은 적응적 형질이다. 점변태곤충류에서는 유충과 성충의 생태학적지위가 중복되여 있어 유충과 성충이 생활공간과 그 밖의 요구조건을 달리하고 있는 완전변태류나 반변태류에 비하여 분산다형성의 예가 대단히 많다. 무시형 또는 단시형곤충은 같은 종의 유시형 또는 장시형곤충에 비하면 초산연령이 빠르고 총산란수도 많은 것이 보통이여서 자연증가율(r)이 크다. 단시형과 관련된 환경요인으로는 서식처의 시간적 영속성이나 공간적 이질성, 먹이조건, 개체군밀도, 온도, 일장 기타 여러 가지가 알려지고 있다 서식처의 환경조건에 대한 분산다형성발현상은 종에 따라 다를 뿐 아니라 암수간에도 차가 있고 같은 종에서도 계통간에 차가 있는 극히 탄력적인 현상이다. 분산다형성의 문제는 생리학, 유전학 그리고 생태학등에 걸친 폭넓은 학문분야로 특히 생태유전학이나 정량유전학분야치 연구는 분산다형성의 유전적본질 구명에 중요하다 하겠다.