• Journal of Biomedical Engineering Research
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• v.29 no.6
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• pp.477-483
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• 2008

The observations of difference and similarity in brain activities involved in processing different languages have fundamental importance in cognitive neuroscience. The purpose of this study was to investigate the difference and similarity in temporal brain activation patterns due to the language difference during visual perception of Korean and English words under priming. Especially, we tried to find the difference in evoked spectral power in gamma-band, which is known to reflect feature binding. The stimulation was visually presented as word pairs belonging to same or different categories so that N400 event-related potential(ERP) was evoked. Average ERP analysis and spectral analysis of gamma-band activity(GBA) were performed on 12 normal Korean subjects. Several ERP components such as P1, N1, N400, and P600 could be identified consistently, and the differences in N1, N400, and P600 were observed. From the spectral analysis, we found that the evoked GBA(eGBA) was significantly larger for English at ${\sim}100$ ms poststimulus. The latency of the eGBA was also considerably delayed for English. Overall, the results on the ERP components and eGBA analyses seem to be commensurate with subjects' familiarity of each language, and the difficulty of perceiving words of each language. The methods of this study can also be applied for clinical purposes considering that the language-related processing can be greatly altered for the patients with neurological or psychiatric diseases.

• BMB Reports
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• v.35 no.5
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• pp.508-512
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• 2002

Phospholipase C-gamma-2 ($PLC{\gamma}2$) activation is a key signaling event for many cell functions. In order to delineate the pathways that lead to $PLC{\gamma}2$ activation, we devised a quick method for obtaining sufficient $PLC{\gamma}2$. We obtained the full-length cDNA for human $PLC{\gamma}2$ and expressed it in E. coli using the expression vector pT5T. To enhance the protein expression, tandem AGG-AGG arginine codons at the amino acid positions 1204-1205 were replaced by CGG-CGG arginine codons. The protein expression was detected in a Western blot analysis by both anti-$PLC{\gamma}2$ antibodies and the antibodies that are raised against the tripeptide epitope (Glu-Glu-Phe) tag that are genetically-engineered to its carboxyl terminal. Crude lysates that were prepared from bacteria that express $PLC{\gamma}2$ were found to catalyze the hydrolysis of phosphatidylinositol 4,5 bisphosphate. Similar to previous reports on $PLC{\gamma}2$ that is isolated from mammalian tissue, the recombinant enzyme was $Ca^{2+}$ dependent with optimal activity at 1-10 uM $Ca^{2+}$.

• Molecules and Cells
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• v.38 no.8
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• pp.697-704
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• 2015

Deleted in breast cancer-1 (DBC1) contributes to the regulation of cell survival and apoptosis. Recent studies demonstrated that DBC is phosphorylated at Thr454 by ATM/ATR kinases in response to DNA damage, which is a critical event for p53 activation and apoptosis. However, how DBC1 phosphorylation is regulated has not been studied. Here we show that protein phosphatase 4 (PP4) dephosphorylates DBC1, regulating its role in DNA damage response. PP4R2, a regulatory subunit of PP4, mediates the interaction between DBC1 and PP4C, a catalytic subunit. PP4C efficiently dephosphorylates pThr454 on DBC1 in vitro, and the depletion of PP4C/PP4R2 in cells alters the kinetics of DBC1 phosphorylation and p53 activation, and increases apoptosis in response to DNA damage, which are compatible with the expression of the phosphomimetic DBC-1 mutant (T454E). These suggest that the PP4-mediated dephosphorylation of DBC1 is necessary for efficient damage responses in cells.

• The Korean Journal of Emergency Medical Services
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• v.18 no.3
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• pp.7-18
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• 2014

Purpose: We aimed to investigate trunk angle and muscle activation of the extremity and back to evaluate the effect of chest compression on work-related musculoskeletal disorders in 119 emergency medical technicians (EMTs). Methods: Eighteen 119 EMTs performed 2-minute chest compression without interruption on a cardiopulmonary resuscitation manikin, during which we measured changes in the trunk and shoulder joint angles, muscle activation (triceps brachii, biceps brachii, erector spinae, gluteus maximus, pectoralis major, rectus abdominis, and rectus femoris) and chest compression accuracy. Results: The decrease in trunk angle by trunk muscle activation was the highest in event 2, the major direction of chest compression. Both shoulder joint angles had no significant difference. Muscle activation of the triceps brachii (p < .01), biceps brachii (p < .05), rectus abdominis (p < .05) and rectus femoris (p < .01) significantly increased during the compression phase compared with the decompression phase, with the rectus femoris showing an increase of 19%. Muscle activation of the erector spinae significantly increased in the decompression phase compared with the compression phase (p < .01). Conclusion: 119 EMTs mainly use the triceps brachii, biceps brachii and pectoralis major muscles during chest compression.

• Korean Journal of Exercise Nutrition
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• v.16 no.2
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• pp.93-100
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• 2012

Alzheimer's disease (AD) is the most common cause of dementia in adults. Microtubule associated protein tau is abnormally phosphorylated in AD and aggregates as paired helical filaments (PHFs) in neurofibrillary tangles (NFTs). NFTs are the most common intraneuronal inclusion in the brains of patients with AD and have been implicated in mediating neuronal cell death and cognitive deficit. Aberrant phosphorylation of tau is an early pathological event in AD, but the underlying mechanisms are unclear. MAP kinases are a family of Serine/Threonine (Ser/Thr) kinases that involved hyper - phosphorylation of tau in AD. The purpose of this study was to investigate the effect of treadmill exercise on phosphorylation of tau level and activation of MAPKs including JNK, ERK, p38-MAPK. To address this, Tg mouse model of AD, Tg-NSE/hTau 23, which expresses human tau 23 in the brain, was chosen. Animals were subjected to treadmill exercise for 12 weeks from 24 months of age. Treadmill exercise in Tg group improved cognitive function compared with Tg-SED group in watermaze test. In addition, treadmill exercised Tg mice significantly reduced the activation of JNK54/46, p38-MAPK and tau (Ser404, Ser202, Thr231), and increased activation of ERK44/42 in cerebral cortex. These results suggest that treadmill exercise may provide a therapeutic potential to alleviate the tau pathology like AD.

• Korean Institute of Interior Design Journal
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• v.16 no.2 s.61
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• pp.339-346
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• 2007

This thesis has purpose of explaining that light is not only appreciated but also physically percepted in contemporary space design and examining effects of space experience through the physical perception. In keeping with such developments, light has been perceived as the object of another architectural tool in modern space design and has provided men with new experience by providing space image and function different from previous ones. With the expansion of the area of light, light has changed a space that is not a simple architectural factor into the essential concept that gives the architectural space image ! by combining it with architectural space, structure, form and materials throughout the whole process from the beginning to the completion. Through phenomenological approach, light is percepted and experienced by body movement, intervention of senses and memories, and passing of time. The percepted and experienced light brings increase of mutual understanding in space, activation and continuance of user's sensation, recreational experience by event occurrence and space experience effect thorough information from media.

• Journal of Korean Society of Rural Planning
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• v.5 no.1 s.9
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• pp.12-19
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• 1999

This study proposes the following directions on the basis of results gained from a survey in Yamanasihyun Kawaguchikocho, where the herb festival is successfully held, in order to activate the local economy by herb. 1) The herb education for person in charge in the local authorities, the promoter of herb festival, has to be preceded and the local residents should try to understand what the herb is. 2) For a successful herb festival in the aspect of local economy and environmental conservation, detailed basic study and publicity including human -, natural environment, ecological conditions for herb should be peformed. 3) Herb event should have various programs and formats to arouse the user's interest and should be a place for environmental education.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.15 no.2
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• pp.117-139
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• 2004

The purpose of this study is that how the housewives' participations of a cultural program that has carried out in a public library influence on their families. So I investigated and analyzed that influences through the research questionnaires to those housewives in Seoul, Kyoung-Ki and In-Cheon as the subject of investigation. I adjusted the focus to make the basic data for the better development and activation of that cultural programs.

• Proceedings of the Korea Contents Association Conference
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• 2010.05a
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• pp.292-296
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• 2010

MICE 산업은 미래의 고부가가치 산업으로서 전략적으로 육성되고 있다. 특히 '광역경제권 5+2'에서 MICE산업은 선도산업으로 지정되었고 경쟁력 있는 인센티브 투어와 리조트형 컨벤션 상품개발 및 판매를 목표로 세부전략들이 추진되고 있다. 그 중 MICE 상품은 테마파티, 팀빌딩, 이벤트 공연 등의 활성화를 위한 방안이 주목되고 있다. 따라서 본 연구에서는 테마파티, 팀빌딩, 이벤트 공연의 수요조사를 통해 실태를 조사하고 활성화 방안을 제언하고자 한다. 이를 위해 문헌고찰을 통한 이론 고찰과 회의참석자 대상 자료수집을 통해 실증분석을 하고자 한다. 마지막으로 도출된 결과를 토대로 관광콘텐츠 측면을 고려한 시사점 및 활용방안을 제시하고자 한다.

• Toxicological Research
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• v.20 no.1
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• pp.63-69
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• 2004

Casapse-3 protease is known as a key role of apoptotic enzyme, and caspase-3 activity is a central event that occurs upstream of DNA fragmentation during apoptosis. This study demonstrates that chitosan pretreatment inhibits cadmium-induced apoptosis by attenuating the activity of caspase-3. We also analyzed the protective effect of chitosan on DNA fragmentation induced by cadmium. Cadmium toxicity was examined by DNA fragmentation and nuclear condensation with Hoechst stain. Caspase-3 activities were increased cadmium treated group for 3 hours compared with control. When chitosan (150 mg/ml) was pretreated at 30 min before cadmium treatment, cadmium cytotoxicity was suppressed in a dose-dependent manner evaluated by DNA fragmentation and caspase activity. From these results, it is suggest that the protective effect of chitosan pretreatment against cadmium-induced cytotoxicity is mediated through inhibition of caspase-3 protease activation and DNA fragmentation.