• 한국임상수의학회지
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• 제31권4호
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• pp.267-271
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• 2014

개 복제는 성숙된 중기의 난자를 수술적으로 회수하여 바로 사용해야 하기 때문에 다른 번식 보조술에 비하여 체내 난자 성숙의 정확한 예측이 특히 더 중요하다. 따라서 본 연구는 개에서 체내 성숙 난자 회수 시 방사면역분석기법(RIA)과 비교하여 화학발광효소면역분석기법(CLEIA)의 신뢰성을 평가하고, 참고값을 설정하기 위해 실시되었다. 배란일(Day 0) 결정을 위하여 발정전기와 발정기의 혈청 프로게스테론 농도가 RIA와 CLEIA 방법으로 분석되었다. Day 3에 수술적인 방법으로 체내 난자를 회수하였고, bisbenzimidazole로 핵을 염색한 뒤 성숙을 현미경으로 평가하였다. 평균 호르몬 농도는 CLEIA 값 ($7.64{\pm}0.06ng/ml$)이 RIA 값 ($6.46{\pm}0.04ng/ml$, P < 0.0001)보다 유의적으로 높았다. Day 0일 때 CLEIA 값 ($10.01{\pm}0.34ng/ml$)은 RIA 값 ($7.91{\pm}0.14ng/ml$)과 다르지 않았으나, Day -1과 Day 1일 때는 CLIEA ($6.41{\pm}0.15$ and $14.25{\pm}0.44ng/ml$)가 RIA($4.95{\pm}0.10$ and $11.29{\pm}0.34ng/ml$)보다 유의적으로 더 높은 값을 나타내었다. 그러나 두 가지 방법 모두 Day -1에서 Day 2까지 프로게스테론 농도가 유의적으로 점차 증가하였다. 그러므로 CLEIA 방법으로 난자 성숙을 결정하기 위해서는 더 넓은 범위와 높은 참고 값이 고려되어야만 한다.

• 한국가축번식학회지
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• 제21권3호
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• pp.255-264
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• 1997

The purpose of this study was attempted to investigate the number changes of the growing and mature follicles in ovary following repeats of pregnant mare serum gonadotropin(PMSG) treatments for superovulation in nulliparous rats. Thirty two rats(Sprague-Dawely, about 200~250 gm) were randomized into 4 groups. Control group rats were sacrified at estrus phase confirmed by vaginal smear. PMSG-treated group 1 rats, PMSG-treated group 2 rats and PMSG-treated group 3 rats were sacrified at 48 hrs after injection once with PMSG 25 IU, after 2 repeated injection by a week interval, and 3 repeated injection, respectively. The uteri and ovaries of rats were removed and weighed and then were observed grossly and serial sections of all ovaries and some sections of uteri by paraffin embedding were stained with H-E. Number of ovarian follicles about 3 grades of small, middle and large follicles from seondary and follicles were investigated by LM photographies of ovary preparations. The criteria of the small, middle, and large follicles were based as small follicle with preantral follicles with 2~4 layers of granulosa cells surrounding the oocyte, as secondary follicles with more than 5 layers of granulosa cells and early signs of antral cavity or with small clefts on either side of the oocytes, and as tirtiary follicles with a single medium sized antral cavity or large well-formed antral cavity, respectively. In gross findings, the wall of the uteri in control group were thin, and those in 3 PMS-treated group were markedly thickened and some uterine lumen of those filled with fluid. In histological findings, the walls of the uteri from 3 PMSG-treated groups were hypertrophied and their blood and lymph vessels were dilated than those of control group. The ovaries fo 3 PMSG-treated groups were more increased in size and the cortexes were more developed and increased in width but there are no difference of development and changes in 3 PMSG-treated groups. The weight of the uteri and ovaries per rat in PMSG -treated group 1, 2 and 3 were a, pp.ared to be significantly increased 171.4$\pm$47.6%, 162.3$\pm$43.9%, 206.9$\pm$30.4%, respectively than those of control groups. The mean number of follicle per ovary in control group were a, pp.ared to be 17.1$\pm$3.5, 46.2$\pm$14.5, and 74.3$\pm$22.7 at large, middle and small follicles, respectively and total number of these 3 grade follicles per ovary were a, pp.ared to be 137.7$\pm$31.7. The mean number of follicle per ovary in PMSG-treated group 1 were a, pp.ared to be 25.6$\pm$7.3, 78.1$\pm$29.9, and 83.2$\pm$34.0, at large, middle and small follicles, respectively and total number of these 3 grade follicles were a, pp.ared to be 187.5$\pm$58.8. The mean number of follicle per ovary in PMS-treated group 2 were a, pp.ared to be 21.9$\pm$5.2, 67.8$\pm$16.8, and 68.0$\pm$14.9 at large, middle and small follicles, respectively and total number of these 3 grade follicles were a, pp.ared to be 157.7$\pm$26.2. The mean number of follicle per ovary in PMS-treated group 3 were a, pp.ared to be 21.7$\pm$4.8, 61.5$\pm$17.0, and 59.7$\pm$16.2 at large, middle and small follicles, respectively and total number of these 3 grade follicles were a, pp.ared to be 143.5$\pm$29.6. The number of follicles in PMSG-treated group 1 a, pp.ared to be more number than other 2 PMSG-treated gruops and tended to be decreased by frequency of PMSG-treatment.