• Journal of Microbiology and Biotechnology
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• 제7권2호
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• pp.132-137
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• 1997

A novel protein (HEAAE, High Essential Amino Acid Encoding Protein), rich in essential amino acids ($75{\%}$ of total), was designed and constructed in our laboratory. The designed peptides were analyzed by SYBLE and stable secondary and tertiary structures were predicted. The monomeric form (HEAAE-1) of the protein consists of 20 amino acid residues with four additional amino acids comprising a potential ${\beta}$-turn (HEAAE-4). Size exclusion analysis demonstrated that the monomer is self-aggregates in aqueous solution to form higher ordered multimeric structures, which are very reminiscent of natural plant storage proteins. The DNA encoding this amino acid sequence was synthesized, and from this monomeric gene fragment (heaae-1), the stable tetrameric form of the gene (heaae-4) was generated by subcloning into the E. coli expression vector pKK223-3. A clear 6 kDa polypeptide band corresponding to the molecular weight of the dimeric form (HEAAE-2) was detected. The smeared band which appeared around the molecular weight corresponding to HEAAE-4 of 11 kDa suggested that the tetramer form of this protein might be processed into smaller size products.

• 한국작물학회지
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• 제45권2호
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• pp.128-133
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• 2000

This study was carried out to investigate the effect of forms and levels of nitrogen fertilizer on plant growth and essential oil production of Agastache rugosa. Calcium nitrate had more influenced on length and width of leaves and lateral branch length than did urea. When nitrogen fertilizer level was increased from 12 kgN/I0a to 24kgN/I0a, plant growth was stimulated and dry matter of leaf and inflorescence were increased. Top dry matter of plant with calcium nitrate treatment (38.4 g) was heavier than that of urea treatment (32.8 g). Interactions among accession and nitrogen form and nitrogen rate were not significantly different for top dry matter. The forms and rate of nitrogen fertilizer did not affect estragole content. The estragole contents was higher in leaf (91.8%) than that of inflorescence (81.3%). While the essential oil content was not affected by different nitrogen forms, nitrogen level affected the essential oil contents positively by increasing dry matter. Essential oil yield was not affected by accession or nitrogen form, but by nitrogen rate. With increasing N application from 12kgN/I0a to 24 kgN/I0a, essential oil yield was increased by 95.8 %.

• 한국식품영양학회지
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• 제20권4호
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• pp.384-391
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• 2007

The purpose of this study was to investigate the preference determinants of buddhist temple food(BTF) and leaded to its activation based on these results. 354 subjects were participated in the survey using questionnaires. 66.9% of subjects had over the normal interest. The taste of BTF was light(34.7%) and it would be helpful in their health(72.9%), subjects answered. Lightness and not strong taste was the main reason of preferring BTF(50.8%), but lack of nutrients was dislike reason of BTF generally. According to factor analysis, the factors of preference determinants deduced as the external form, social environment, health, essential quality and information of food. There were not significantly different between factors by sex and family form. External form, health and essential quality of food had the significant difference by generation and education level. The factors of external form and the essential quality of food showed the significant difference by job. According to the monthly income, the factor of social environment, health and information of food had the significant difference.

• Asian-Australasian Journal of Animal Sciences
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• 제25권8호
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• pp.1138-1144
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• 2012

This study was conducted to evaluate the efficacy of the dipeptide form of phenylalanine as a new source of amino acid in terms of growth performance and whole-body amino acid composition in comparison to the free form for red seabream (Pagrus major). Fish ($1.46{\pm}0.001g$) were fed four isonitrogenous and isocaloric experimental diets containing 0.7 or 1.4% phenylalanine either in free or dipeptide form. A feeding trial was carried out in three replicates and the fish were fed to apparent satiation for six weeks. At the end of the feeding trial, feed intake of fish was influenced by both phenylalanine form and level and significantly higher values were obtained at an inclusion level of 0.7% and by the use of dipeptide form. However, the other growth parameters did not significantly differ among treatments. Whole-body amino acid compositions revealed no significant changes in concentrations of both essential and non-essential amino acids regardless of the increase in phenylalanine levels or the use of its different forms. The finding in this study indicates that juvenile red seabream can utilize dipeptide phenylalanine as efficiently as free form without any undesirable effects on growth performance or whole-body amino acid composition.

• 대한전기학회논문지
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• 제39권12호
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• pp.1288-1295
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• 1990

In this paper, we propose a parallel processing model for the efficient selection of Prime Implicants of Logic Functions. This model consists of simple parallel processing nodes, connections between them, Max Net (a part of Hamming Net) and quasi essential Prime Implicant selection standard in simplified cost form. Through these, this model selects essential Prime Implicants in a certain period of time regardless of the number of given Prime Implicants and minterms and also selects quasi essential Prime Implicants in short time.

• 헤리티지:역사와 과학
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• 제49권1호
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• pp.146-165
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• 2016

1970년 중요무형문화재 보유자 인정제도가 도입되면서 '원형규범'이 채택되었다. 규범으로 채택되기 이전에도 '원형'은 문화재 보존의 방향으로 통용되고 있었다. 법규상 원형의 개념은 형태적인 개념이다. 그러나 구전 및 실연에 의해 전승되어온 무형문화재의 특성상 과거 어느 시점에 누구에 의해 실연되는 것을 원형의 형태로 확인하거나 특정할 수 없으므로, 지정 인정행위의 시점에서 현존인이 실연하는 기 예능을 중심으로 원형을 설정하고 보유자를 인정하였다. 따라서 근원적 실체로서 '원형' 이 아닌 지정 인정 당시의 보유 기 예능이 '잠정적 원형'으로 설정되면서 '원형보존' 정책이 시행되었다. 원형보존 정책은 '원형'을 보유한 보유자의 전수교육과 전승자 양성을 통하여 시행되었으며, 보유자(보유단체)를 중심으로 한 배타적인 전승환경이 조성되기에 이른다. 그러나 이러한 원형보존 정책에도 불구하고 지정 당시의 기 예능은 사회적 환경과 수요에 맞게끔 변화가 진행되어 보존하고자 했던 형태적 의미의 '원형'은 보존되지 않았다. 그럼에도 불구하고 원형규범은 근대화, 서구화 등 외부환경으로부터 우리 전통문화를 보존하기 위한 사회적 실천의 지침 내지는 지향점을 지닌 시대적 담론이었으며, 무형문화재 보존정책의 당위적 지침으로 정책적 실효를 거두었다고 평가할 수 있다. 2016년 3월 시행될 예정인 무형법은 무형문화재의 변화적 속성을 고려하여 '원형' 대신 '전형'을 도입하였다. '전형'은 무형문화재 주변부의 변화를 인정하여 원형의 경직성을 탈피하고자 한 것이다. 그러나 법규상 '전형'에 해당하는 고유한 가치, 지식, 기법이 문화재 보존의 지침 또는 원칙에 따라 유지되어야 하므로 전형은 원형과의 단절보다는 관련성과 연장선에서 볼 수도 있다.

• 한국실내디자인학회논문집
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• 제17호
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• pp.37-44
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• 1998

Chair is one of objects which are closely connected with human body and is a symbol which represents human essence and culture. Symbolism embodies the meaning of expression through transfering the immaterial structure to tangible form. Therefore chair embodies the essential internal desires of human by means of its symbolic representation. In order to understand the essential human desires included in the symbolism of chair it is necessary to consider these desires with respect to cultural and historical context. in this study four essential internal desires of human are investigated through the symbolic representation of chair that is the desires on human psychological comfort authority freedom and eroticism. The purpose of this study is to emphasize the necessity of the design approach based on the understanding of essential internal desires of human which is pursued from the object by user.

• 건축역사연구
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• 제8권3호
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• pp.39-51
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• 1999

The purpose of this study is to establish the theory of the spatial composition that is 'The Structure of Relation' in Korean traditional houses. This study has been focused on the spatial composition of Hyangdan. The form composition is an element defining primitive functions and space and producing aesthetical effects, where 'quality as a substance' is not important but 'quality as a relation' is essential. Quality as a meaningful relationship comes from realization and regulation of visual attributes among form elements. Each form compositional element establishes the hierarchical structure logically with the entire order, In order for the quality of all form compositional elements to be clarified as a meaningful relationship logically, 'compositional concept' which combines a series of form elements into the grammatical dependence to a specific direction, has to be assumed. If the intrinsic relationship among a series of form compositional elements fails to confront with the 'contextual concept' which eventually indicates unique conditions for a place according to the refined compositional concept, the result of form composition never leads to a specific solution

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 1991년도 봄 학술발표회 논문집
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• pp.43-47
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• 1991

3차원 자유형상 구조물인 선박, 비행체, 자동차 등의 최적형상 결정에 관한 구조해석을 위해서는 원하는 설계조건을 만족시키는 범위내에서 자유형상 구조물의 다양한 형상의 정의와 비교가 필요하다. 본 연구에서는 형상계수를 이용한 3차원 자유형상 구조물의 정의와 정의된 구조물의 체계적인 변화 및 구조해석을 위한 기하학적 형상정보의 도출을 시도하고, 이의 검증을 위해 선정한 자유형상 모델을 통해 이 방법의 유용성을 조사했다.

• 전산구조공학
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• 제5권2호
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• pp.79-85
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• 1992

3차원 자유형상 구조물인 선박, 비행체, 자동차 등의 최적형상 결정에 관한 구조해석을 위해서는 원하는 설계조건을 만족시키는 범위내에서 자유형상 구조물의 다양한 형상의 정의와 비교가 필요하다. 본 연구에서는 형상계수를 이용한 3차원 자유형상 구조물의 정의와 체계적인 변화 및 구조해석을 위한 기하학적 형상정보의 도출을 시도하고, 이의 검증을 위해 선정한 자유형상 모델을 통해 이 방법의 유용성을 조사했다.