• Journal of Microbiology and Biotechnology
• /
• v.7 no.2
• /
• pp.132-137
• /
• 1997

A novel protein (HEAAE, High Essential Amino Acid Encoding Protein), rich in essential amino acids ($75{\%}$ of total), was designed and constructed in our laboratory. The designed peptides were analyzed by SYBLE and stable secondary and tertiary structures were predicted. The monomeric form (HEAAE-1) of the protein consists of 20 amino acid residues with four additional amino acids comprising a potential ${\beta}$-turn (HEAAE-4). Size exclusion analysis demonstrated that the monomer is self-aggregates in aqueous solution to form higher ordered multimeric structures, which are very reminiscent of natural plant storage proteins. The DNA encoding this amino acid sequence was synthesized, and from this monomeric gene fragment (heaae-1), the stable tetrameric form of the gene (heaae-4) was generated by subcloning into the E. coli expression vector pKK223-3. A clear 6 kDa polypeptide band corresponding to the molecular weight of the dimeric form (HEAAE-2) was detected. The smeared band which appeared around the molecular weight corresponding to HEAAE-4 of 11 kDa suggested that the tetramer form of this protein might be processed into smaller size products.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.45 no.2
• /
• pp.128-133
• /
• 2000

This study was carried out to investigate the effect of forms and levels of nitrogen fertilizer on plant growth and essential oil production of Agastache rugosa. Calcium nitrate had more influenced on length and width of leaves and lateral branch length than did urea. When nitrogen fertilizer level was increased from 12 kgN/I0a to 24kgN/I0a, plant growth was stimulated and dry matter of leaf and inflorescence were increased. Top dry matter of plant with calcium nitrate treatment (38.4 g) was heavier than that of urea treatment (32.8 g). Interactions among accession and nitrogen form and nitrogen rate were not significantly different for top dry matter. The forms and rate of nitrogen fertilizer did not affect estragole content. The estragole contents was higher in leaf (91.8%) than that of inflorescence (81.3%). While the essential oil content was not affected by different nitrogen forms, nitrogen level affected the essential oil contents positively by increasing dry matter. Essential oil yield was not affected by accession or nitrogen form, but by nitrogen rate. With increasing N application from 12kgN/I0a to 24 kgN/I0a, essential oil yield was increased by 95.8 %.

• The Korean Journal of Food And Nutrition
• /
• v.20 no.4
• /
• pp.384-391
• /
• 2007

The purpose of this study was to investigate the preference determinants of buddhist temple food(BTF) and leaded to its activation based on these results. 354 subjects were participated in the survey using questionnaires. 66.9% of subjects had over the normal interest. The taste of BTF was light(34.7%) and it would be helpful in their health(72.9%), subjects answered. Lightness and not strong taste was the main reason of preferring BTF(50.8%), but lack of nutrients was dislike reason of BTF generally. According to factor analysis, the factors of preference determinants deduced as the external form, social environment, health, essential quality and information of food. There were not significantly different between factors by sex and family form. External form, health and essential quality of food had the significant difference by generation and education level. The factors of external form and the essential quality of food showed the significant difference by job. According to the monthly income, the factor of social environment, health and information of food had the significant difference.

• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.8
• /
• pp.1138-1144
• /
• 2012

This study was conducted to evaluate the efficacy of the dipeptide form of phenylalanine as a new source of amino acid in terms of growth performance and whole-body amino acid composition in comparison to the free form for red seabream (Pagrus major). Fish ($1.46{\pm}0.001g$) were fed four isonitrogenous and isocaloric experimental diets containing 0.7 or 1.4% phenylalanine either in free or dipeptide form. A feeding trial was carried out in three replicates and the fish were fed to apparent satiation for six weeks. At the end of the feeding trial, feed intake of fish was influenced by both phenylalanine form and level and significantly higher values were obtained at an inclusion level of 0.7% and by the use of dipeptide form. However, the other growth parameters did not significantly differ among treatments. Whole-body amino acid compositions revealed no significant changes in concentrations of both essential and non-essential amino acids regardless of the increase in phenylalanine levels or the use of its different forms. The finding in this study indicates that juvenile red seabream can utilize dipeptide phenylalanine as efficiently as free form without any undesirable effects on growth performance or whole-body amino acid composition.

• The Transactions of the Korean Institute of Electrical Engineers
• /
• v.39 no.12
• /
• pp.1288-1295
• /
• 1990

In this paper, we propose a parallel processing model for the efficient selection of Prime Implicants of Logic Functions. This model consists of simple parallel processing nodes, connections between them, Max Net (a part of Hamming Net) and quasi essential Prime Implicant selection standard in simplified cost form. Through these, this model selects essential Prime Implicants in a certain period of time regardless of the number of given Prime Implicants and minterms and also selects quasi essential Prime Implicants in short time.

• Korean Journal of Heritage: History & Science
• /
• v.49 no.1
• /
• pp.146-165
• /
• 2016

With the introduction of the system of recognizing masters of craft and performance skills in 1970, the principle of "preserving the original form," which was already in general use, was adopted as a legal principle in the Cultural Heritage Protection Act. While the concept "original form" can be related to tangible elements of heritage through the Act, the intangibility of craft and performance skills does not allow their pinpointing at a particular temporal period or the identification of a particular master from the past as the basis of an original form. Therefore, those craft or performance skills that are available at the point of recognition of relevant masters must serve as the basis of the original form for the intangible heritage concerned. This means that the principle of preserving the original form of intangible heritage has been implemented not based on a fundamental form of materiality, but rather on the craft or performance skills that may be held by a master at the time of his/her recognition as a "temporary original form." This principle has been observed through intangible heritage transmission and education policies for recognized masters and their trainees, contributing to establish an elitist transmission environment in which public were denied to join the education on intangible heritage. Even with policies guided by the principle of preserving the original form, designated craft and performance skills have been transformed contingent upon given social and environmental conditions, thus hindering the preservation of the original form. Despite the intrinsic limitations of the principle of preserving the original form when applied to intangible heritage, this principle has served as a practical guideline for protecting traditional Korean culture from external influences such as modernization and Westernization, and also as an ultimate goal for the safeguarding of intangible heritage, engendering actual policy effects. The Act on the Safeguarding and Promotion of Intangible Cultural Heritage that comes into effect in March 2016 takes the constantly evolving nature of intangible heritage into consideration and resultantly adopts a concept of "essential form" (jeonhyeong) in place of "original form" (wonhyeong). This new concept allows for any transformations that may take place in the environment surrounding the intangible heritage concerned, and is intended to mitigate the rigidity of the concept of "original form." However, it should be noted that "essential form," which is manifested as the unique significance, knowledge, and skills delivered by the intangible heritage concerned, should be maintained according to the guidelines and principles related to heritage conservation. Therefore, the new concept can be understood not as a rupture, but more as a continuum of the concept of "original form."

• Korean Institute of Interior Design Journal
• /
• no.17
• /
• pp.37-44
• /
• 1998

Chair is one of objects which are closely connected with human body and is a symbol which represents human essence and culture. Symbolism embodies the meaning of expression through transfering the immaterial structure to tangible form. Therefore chair embodies the essential internal desires of human by means of its symbolic representation. In order to understand the essential human desires included in the symbolism of chair it is necessary to consider these desires with respect to cultural and historical context. in this study four essential internal desires of human are investigated through the symbolic representation of chair that is the desires on human psychological comfort authority freedom and eroticism. The purpose of this study is to emphasize the necessity of the design approach based on the understanding of essential internal desires of human which is pursued from the object by user.

• Journal of architectural history
• /
• v.8 no.3 s.20
• /
• pp.39-51
• /
• 1999

The purpose of this study is to establish the theory of the spatial composition that is 'The Structure of Relation' in Korean traditional houses. This study has been focused on the spatial composition of Hyangdan. The form composition is an element defining primitive functions and space and producing aesthetical effects, where 'quality as a substance' is not important but 'quality as a relation' is essential. Quality as a meaningful relationship comes from realization and regulation of visual attributes among form elements. Each form compositional element establishes the hierarchical structure logically with the entire order, In order for the quality of all form compositional elements to be clarified as a meaningful relationship logically, 'compositional concept' which combines a series of form elements into the grammatical dependence to a specific direction, has to be assumed. If the intrinsic relationship among a series of form compositional elements fails to confront with the 'contextual concept' which eventually indicates unique conditions for a place according to the refined compositional concept, the result of form composition never leads to a specific solution

• Proceedings of the Computational Structural Engineering Institute Conference
• /
• 1991.04a
• /
• pp.43-47
• /
• 1991

For the structural analysis of optimum form decision of 3-D free fort structure such as ship, plane, automobile, definition of versatile forms and comparision between them satisfying the design criterion, is essential. In this paper, three dimensional free-form structure and it's variation are defined and attempts were made to obtain geometric form information for structural analysis. The validity of the method ks been tested for a particular free-form model selected.

• Computational Structural Engineering
• /
• v.5 no.2
• /
• pp.79-85
• /
• 1992

For the structural analysis of optimum form decision of 3-D free form structure such as ship, plane, automobile, definition of versatile forms and comparision between them satisfying the design criterion, is essential. In this paper, 3-D free form structure and it's variation are defined and attempts were made to obtain geometric form information for structural analysis. The validity of the method has been tested for a particular free model selected.