• Title/Summary/Keyword: Erythrosin B

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Vital and Acrosomal Staining of Bovine Spermatozoa (소 정자의 생존율 및 첨체반응 검사를 위한 간단한 염색법)

  • 김계성;이병천;황우석
    • Journal of Embryo Transfer
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    • v.15 no.1
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    • pp.103-108
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    • 2000
  • The object of this study was to find simple and effective methods for the speculation of vitality and scorsome status of bovine spermatozoa. The eosin-nigrosin staining, trypan blue staining, and naphthol yellow S-erythrosin B staining was ofter used for the speculation of vitality and/or acrosome status of bovine spermatozoa, respectively. This study has shown that the combined trypan blue-naphthol yellow S-erythrosin B staining is more accurate and effective for the examination of acrosome status and vitality of bovine spermatozoa.

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Aqueous-Based Photocurrent Generation by Fluorescence Resonance Energy Transfer between Conjugated Oligoelectrolytes and Erythrosin B (전도성 올리고전해질과 에리스로신 B간 형광공명 에너지전달에 의한 수용액 기반 광전류 생성)

  • Kang, Insung;Park, Jonghyup;Jo, Hyunjin;Park, Juhyun
    • Polymer(Korea)
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    • v.39 no.2
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    • pp.353-358
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    • 2015
  • Aqueous-based, environmentally friendly photocurrent generation has been highlighted to produce electricity by mimicking photosynthesis in nature. We fabricated a photocurrent generation system using a conjugated oligoelectrolyte (COE) assembled in lipid vesicles and a fluorescence dye, and investigated the fluorescence resonance energy transfer (FRET) between two species and the influence of FRET on the photocurrent generation. The FRET efficiency from the electron donor, COE, to the electron acceptor, the dye, increased with the dye concentrations, but the photocurrent increased and then decreased with the dye concentrations. We discussed about the role of FRET and electron shuttles over the variation of photocurrent.

The effects of dietary photosensitizers on auto-oxidation of gallic and tannic acids (갈산과 타닌산의 자동산화에 미치는 식품 감광성분의 영향)

  • Lee, Eunbin;Lee, Hyowon;Hong, Jungil
    • Korean Journal of Food Science and Technology
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    • v.54 no.3
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    • pp.272-279
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    • 2022
  • Polyphenols are chemically unstable, and their bioactivities are reduced through oxidation. Photosensitizers (PS) induce photo-oxidation in various food systems. In this study, effects of dietary PS such as riboflavin (Rb), erythrosin B (EB), and zinc protoporphyrin on the auto-oxidation of polyphenols, gallic acid (GA) and tannic acid (TA) were evaluated under a fluorescent light. The formation of oxidation products from GA and TA increased in a PS concentration- and irradiation time-dependent manner. In addition, Rb and EB induced significant reduction in the polyphenols contents and ABTS radical scavenging activity of GA and TA under light. PS significantly enhanced the amount of reactive oxygen species generated from GA and TA. Therefore, the interaction of polyphenols with PS under light results in acceleration of polyphenol oxidation. This phenomenon should be carefully considered during food processing and storage.