• Title/Summary/Keyword: Epimedium spp.

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Morphological characteristics and RAPD analysis of Epimedium spp. (국내외 수집 삼지구엽초의 형태적 특성 및 유연관계 분석)

  • Lim, Jung-Dae;Seong, Eun-Soo;Choi, Kwang-Joon;Kim, Seung-Kyung;Chung, Ill-Min;Heo, Kweon;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.8 no.2
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    • pp.102-108
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    • 2000
  • Morphological characteristics of nine Epimedium spp was analyzed on the basis of six morphological characters. To analyse the genetic relationship among Epimedium spp., polymerase chain reaction (PCR) was performed with total genomic DNA of 17 Epimedium spp. by using random 8 primers. The genetic diversity and genetic distance among nine Korean collections, seven Japanese and one China collection were used to generate a dendrogram showing phylogenie relationship. Seventeen Epimedium spp were classified into two groups of group I and II, since they were divided into two major groups at the similarity coefficients value of 0.65. In addition, one of the two group, group I was divided into three sub-groups including Epimedium koreanum, Chul-won collections 1, 2, and 3, Yanggu, Hongchon, Hwachun, Chunchon, China, Maehwa (Japanese), E. diphyllum and E. violaceum (Japanese), while group II included Chulwon collection 4 and 5 and Japanese collection. The samples collected at Chulwon district showed close similarity with Japanese collection. Similarity indexes between collection and genetic relationship were related at the levels ranging from 0.6 to 0.9.

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