• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.602-605
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• 2001

Low-temperature adaptation and protection for environmental stresses were studied in the gram-negative soil bacterium Bradyrhizobium japonicum 61A101c. B. japonicum was more resistant to alcohol, $H_2O_2$, heat and freezing following a pretreatment at $4^{\circ}C$, resulting in approximately 10 to 1,000 folds increased survival compared to mid-exponential-phase cells grown at an optimal temperature at $28^{\circ}C$. This phenomena relate to the cold shock protein expressed when cells are exposed to a downshift in temperature. To confirm the presence of cold shock protein genes in B. japonicum, a PCR strategy was employed using a degenerate primer set, which successfully amplified a putative csp gene fragment. Sequence analysis of the PCR product(200bp) revealed csp-like sequences that were up to 96% identical to csp gene of S. typhimurium.

• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8019-8029
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• 2016

The development of new strategies for vaccine delivery for generating protective and long-lasting immune responses has become an expanding field of research. In the last years, it has been recognized that bacteriophages have several potential applications in the biotechnology and medical fields because of their intrinsic advantages, such as ease of manipulation and large-scale production. Over the past two decades, bacteriophages have gained special attention as vehicles for protein/peptide or DNA vaccine delivery. In fact, whole phage particles are used as vaccine delivery vehicles to achieve the aim of enhanced immunization. In this strategy, the carried vaccine is protected from environmental damage by phage particles. In this review, phage-based vaccine categories and their development are presented in detail, with discussion of the potential of phage-based vaccines for protection against microbial diseases and cancer treatment. Also reviewed are some recent advances in the field of phagebased vaccines.

• International Journal of Automotive Technology
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• 제4권3호
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• pp.141-147
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• 2003

For improving high-safety, convenience, and ride comfort, the automotive design suffers from radical increase of the weight, the recycling-related rules, regulations on the waste gas, and environmental protection of the resources. Among them, it is well known that the weight increase is the most critical. Thus, in order to minimize the weight of the body-in-white that takes up 20-30% of the whole weight of the automobile, most automotive manufacturers have attempted to develop the aluminum intensive body-in-white using aluminum space frames. In this research, the crush test and simulation for aluminum extrusions are performed to evaluate the collapse characteristics of that light weighted material. Also. the same test and simulation was done for aluminum extrusions filled with structural foam. Then, these results are analyzed and compared. From these studies, the effectiveness of structural foam is evaluated in improving automotive crashworthiness. Finally, the design strategy and guideline of the structural form are suggested in order to improve the crashworthiness for aluminum space frame in the vehicle.

• 한국환경복원기술학회지
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• 제2권1호
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• pp.58-65
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• 1999

The purpose of this study was to evaluate the effect of rehabilitation measures on the fired woodland of Chesuk-bong in Mt. Chiri through the vegetation structure and comparison of growth pattern between natural seedlings and transplanted Abies koreana seedlings. It was also discussed the rate of standing and fallen trees of dead conifers in the management strategy for vegetation regeneration and protection of sub-alpine area. In this fired woodland, shrub layers consisting of Weigela subsessilis, Sambucus williamsii var. coreana, Rhododendron schlippenbachii, R. mucronulatum, Tripterygium regelii, and etc. were gradually expanded, and tree species such as Betula ermani, Sorbus commixta, Acer pseudosieboldianum, and Malus baccata var. mandshurica were also regenerated. The growth of height and crown width of Abies koreana seedlings transplanted for vegetation regeneration were a little lower than those of natural seedlings. The seedlings of A. koreana transplanted in this fired woodland grew about 50.6% normally, but the others had multi-branched or growth stopped by death of terminal shoot. Because the number of dead conifers by fire tend to be gradually increased as time passed, it can occurs to the soil erosion and landslide by weakness of the cohesion and resistance of soil. Therefore, it is consider that rehabilitation measures projects of the fired woodland in subalpine area may be more prevention a natural disaster like soil erosion, flood and landslide.

• 한국식품위생안전성학회지
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• 제23권3호
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• pp.276-283
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• 2008

국내 외 선행연구결과에 의한 어린이 출생 전 후 건강에 영향을 미칠 수 있는 주요 유해물질은 inorganic arsenic, BPA, 2,4-D, dichlorvos, methylmercury, PCBs, pesticide, phthalate, PFOA/PFOS, vinyl chloride 등으로서 국내에서도 이들을 포함한 확인 연구가 수행되어져야 할 것이다. 또한 어린이들의 행동발달과 연관된 생활환경에서의 다양한 인체노출이 확인됨에 따라 탁아소, 유치원과 같은 경우 어린이 활동공간을 대상으로 환경모니터링 및 위해관리활동도 병행되어야 할 것이다. 또한 어린이 건강에 영향을 미치는 유해물질을 중심으로 독성평가, 인체영향연구, 환경 및 인체모니터링과 위해평가를 수행하므로 유해물질의 원인-상관관계를 확인하고 사실자료에 근거한 관리정책을 반영하는 것이 바람직하다.

• 한국환경성돌연변이발암원학회지
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• 제26권4호
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• pp.97-112
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• 2006

Heme oxygenase(HO)-1 is an important antioxidant enzyme that plays a pivotal role in cellular adaptation and protection in response to a wide array of noxious stimuli. Thus, HO-1 induction has been associated with prevention or mitigation of pathogenesis of various diseases, including acute inflammation, atherosclerosis, degenerative diseases, and carcinogenesis. Recent progress in our understanding of the function of molecules in the cellular signaling network as key modulators of gene transcription sheds light on the molecular mechanisms underlyuing HO-1 gene expression. A panel of redox-sensitive transcription factors such as activator protein-1, nuclear factor-kB, and nuclear factor E2-related factor-2, and some of the upstream kinases have been identified as prime regulators of HO-1 gene induction. This review summarizes molecular mechanisms underlying HO-1 expression and the significance of targeted induction of HO-1 as a potential chemopreventive or chemoprotective strategy.

• 한국환경복원기술학회지
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• 제25권6호
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• pp.65-75
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• 2022

Urban flood management(UFM) strategy ought to consider the connections and interactions between existing and new infrastructures to manage stormwater and improve the capacity to treat water. It is also important to demonstrate strategies that can be implemented to reduce the flow at flooding sources and minimize flood risk at critical locations. Although the general theory of spatial impact is popular, modeling guidelines that can provide information for implementation in real-world plans are still lacking. Under such background, this study conducted a modeling research based on an actual target site to confirm the hypothesis that it is appropriate to install green infrastructure(GI) in the source area and to take structural protection measures in the impact area, as summarized in previous studies. The results of the study proved the hypothesis, but the results were different from the hypothesis depending on which hydrological performance indicators were targeted. This study will contribute to demonstrating the effectiveness of strategies that can be implemented to reduce the flow at flooding sources and minimize the risk of flooding in critical locations in terms of spatial planning and regeneration.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제3권2호
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• pp.84-96
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• 2022

Most of the islands of Korea are distributed in the South and West Sea, and it consists of independent small stream. As a result, the fish community that inhabits the island's stream is isolated from the mainland and other island. This study utilized a Self-Organizing Map (SOM) and a random forest model to analyze the relationship between environmental variables and fish communities inhabiting islands in South Korea. Through the SOM analysis, the fish communities were divided into three clusters, and there were differences in biotic and abiotic factors between these groups. Cluster I consisted of sites with relatively larger island areas and a higher number of species and population. It was found that 15 out of 16 indicator species were included. Meanwhile, the remaining clusters had fewer species and populations. Cluster II, especially, showed the lowest impact from physical variables such as water width and depth. As a result of predicting the species richness using the random forest model, physical variables in habitats, such as stream width and water depth, had a relatively higher importance on species richness. On the other hand, forest area was the most important variables for predicting Shannon diversity, followed by maximum water depth, and gravel. The results suggest that this study can be used as basic data for establishing a stream ecosystem management strategy in terms of conservation and protection of biological resources in streams of islands.

• 드라이브 ㆍ 컨트롤
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• 제20권3호
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• pp.1-8
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• 2023

The purpose of this study was to investigate and compare the impact of engine load on the emission characteristics of excavator engines, with the aim of improving the method for calculating the emission inventory of construction machinery. The engine load in excavators is directly correlated with the operational workload, and variations in the load factor (LF) can significantly influence the emission inventory. Thus, on-board diagnostic (OBD) data from an excavator at a construction site were systematically collected to measure engine output and emissions. The results revealed discernible differences in emissions based on engine load, even when the average excavator engine performance remained constant. This highlights the significant influence of the type and characteristics of the work being carried out on emission characteristics. Making realistic adjustments to the LF used in emission calculation formulas emerges as a crucial strategy for environmental improvement. Moreover, the analysis of the effects of engine load on emissions from excavators provides valuable insights for enhancing environmental protection measures.

• 융합경영연구
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• 제11권6호
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• pp.35-41
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• 2023

Purpose: This study aims to determine the marketability of the heritage sites in Region III, Philippines. More specifically, it will obtain information on the: (a) profile of the heritage sites in Central Luzon; (b) dimensional issues of their marketability along social, environmental, and economic lines; (c) quality of services in terms of tangibility, responsiveness, empathy, assurance, and reliability; and (d) its business model canvas. Research design, data, and methodology: This will utilize a descriptive survey of the heritage sites in Central Luzon, namely: Aurora, Nueva Ecija, Bulacan, Pampanga, Tarlac, Zambales, and Bataan. There will be 60 respondents, including 5 facilitators, 24 residents, and 31 tourists. The results will be statistically measured through the measures of central tendencies, dispersion, and the test of significance. Result and conclusion: This study will comprehensively examine the local tourism sector, benefiting various stakeholders. It serves as a valuable resource for tourists by providing insights into destination marketing strategies and enhancing heritage tourism experiences. Administrators benefit from coping strategy evaluations, aiding in the formulation of effective strategies aligned with industry goals. Tourism businesses align with industry objectives and the study streamlines ordinances for site protection for local government units. Additionally, the community gains empowerment through insights into employable activities and potential businesses, influencing assessments of the justification for local preservation ordinances.