• Korean Journal of Environmental Biology
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• v.17 no.3
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• pp.263-270
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• 1999

In order to identify the morphological changes of tissues in mouse after irradiation. We have observed the redistribution of LDH isozymes and the morphological changes of skeletal muscle, heart, kidney, liver and testis in mouse according to variation amount with the time after the 1 Gray and 3 Gray irradiation each. As a result of H-E (hematoxylin-eosin) stain, the apoptotic bodies were more easily observed in the liver than the other tissues and the quantity of the apoptotic bodies was proportionated to radiation amount. The number of apoptotic bodies was shown the highest at 1 day in most tissues and at 7 day in testis after irradiation. TUNEL (terminal deoxyribonucleodtidyl transferase-mediated dUTP-digoxigenin nick end labeling) staining was shown the same results as H-E staining. After the irradiation, the protein content was reduced in tissues except kidney. And protein content was reduced in all tissues at the initial period of 2 hours after 3 Gy irradiation. But it increased at 7 days after irradiation. LDH (EC 1.1.1.27, lactate dehydrogenase) activity was increased mostly in tissues at the early stage after 1 Gy irradiation. The maximum activity was detected earlier stage after 1 Gy irradiation than 3 Gy irradiation. The activity of LDH $A_4$ isozyme was decreased in the skeletal muscle, heart, kidney, and testis. The activity of $B_4$ and $A_2$$B_2$ sozyme was increased in the skeletal muscle and heart, and the activity of heterotetramer isozyme was increased in kidney The activity of $A_4$ isozyme in liver was detected high level and the activity of isozyme including subunit C elevated in testis. Therefore, LDH isozyme seems to play a role of lactate oxidase in most tissues except liver after irradiation. These data support that LDH isozyme is predomintly involved in the aerobic metabolism.

• Journal of the Korea Society of Computer and Information
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• v.15 no.2
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• pp.41-46
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• 2010

In this paper, we propose a detection method of reduced computational complexity aimed at separating the moving objects from the background in a generic video sequence. In generally, indoor environments, it is difficult to accurately detect the object because environmental factors, such as lighting changes, shadows, reflections on the floor. First, the background image to detect an object is created. If an object exists in video, on a previously created background images for similarity comparison between the current input image and to detect objects through several operations to generate a mixture image. Mixed-use video and video inputs to detect objects. To complement the objects detected through the labeling process to remove noise components and then apply the technique of morphology complements the object area. Environment variable such as, lighting changes and shadows, to the strength of the object is detected. In this paper, we proposed that environmental factors, such as lighting changes, shadows, reflections on the floor, including the system uses mixture images. Therefore, the existing system more effectively than the object region is detected.

• Clean Technology
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• v.21 no.1
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• pp.33-38
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• 2015

In this research, life cycle inventory database (LCI DB) was developed for liquid CO₂ employing life cycle assessment (LCA) methodology. As are result of characterization and normalization process, production of liquid CO₂ puts on environmental impact in the order of resource depletion, global warming, acidification, eutrophication and photochemical oxidation, and among a wide variety of input, electricity contributes in most of the impact categories. Air emission plays a key role in the acidification and eutrophication while ammonia affects most on the ozone depletion. It is anticipated that development of liquid CO₂ LCI DB makes it possible for national environmental strategies to be more activated including environmental labeling scheme.

• Resources Recycling
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• v.33 no.2
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• pp.62-72
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• 2024

Although the Paris Agreement in 2015 aimed to limit global temperature increases to below 2℃ and eventually to 1.5℃ to address the climate crisis, global temperature continues to rise. Developed countries have proposed a circular economy as a major strategy to tackle this issue. Detailed implementation methods include reusing, remanufacturing, recycling, and energy recovery. Remanufacturing has a greater potential to achieve high added value and carbon neutrality than other resource circulation methods. However, currently, no standardized method for quantitatively evaluating the greenhouse gas (GHG) reduction effects of remanufacturing exists. This study compares and analyzes recent research trends since 2020 on the calculation of GHG emission reduction effects from remanufacturing. It also examines international standards for environmental impact assessment, including GHGs and environmental performance labeling systems. This study derives the key factors for standardizing the calculation of the GHG emission reduction effects of remanufactured products.

• Journal of Korea Multimedia Society
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• v.19 no.2
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• pp.240-251
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• 2016

This paper presents a real-time indoor location and pose estimation method that utilizes simple artificial markers and image analysis techniques for the purpose of warehouse automation. The conventional indoor localization methods cannot work robustly in warehouses where severe environmental changes usually occur due to the movement of stocked goods. To overcome this problem, the proposed framework places artificial markers having different interior pattern on the predefined position of the warehouse floor. The proposed algorithm obtains marker candidate regions from a captured image by a simple binarization and labeling procedure. Then it extracts maker interior pattern information from each candidate region in order to decide whether the candidate region is a true marker or not. The extracted interior pattern information and the outer boundary of the marker are used to estimate location and heading angle of the localization system. Experimental results show that the proposed localization method can provide high performance which is almost equivalent to that of the conventional method using an expensive LIDAR sensor and AMCL algorithm.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.119.1-119
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• 2003

The glyoxysomal nature of microbodies was determined in Botryosphaeria dothidea hyphae based on morphology and in situ enzyme characteristics by transmission electron microscopy and cytochemistry. Bound by a single membrane, microbodies had a homogeneous matrix and varied in size ranging from 200 to 400 m in diameter. Microbodies had crystalline inclusion(s) which consisted of parallel arrays of fine tubules in their matrices. Microbodies and lipid globules were frequently placed in close association with each other, forming microbody-lipid globule complexes in hyphae. The cytochemical activities of catalase and malate synthase were localized in matrices of microbodies, showing intense electron-density of the organelle. In addition, the immunogold labeling detected the presence of catalase in multivesicular bodies and hyphal cell walls as well as in matrices and crystalline inclusions of microbodies, supporting the enzyme secretion through cell walls. Meanwhile, isocitrate Iyase was localized only in matrices of microbodies. These results suggest that microbodies, particularly complexed with lipid globules, in the fungal hyphae are functionally defined as glyoxysomes, where glyoxysomal enzymes are biochemically active for the glyoxylate cycle to be a metabolic pathway in gluconeogenesis. (Mycology and Fugus Diseases)

• Mass Spectrometry Letters
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• v.4 no.1
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• pp.1-9
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• 2013

Fungal biotechnology has been well established in food and healthcare sector, and now being explored for lignocellulosic biorefinery due to their great potential to produce a wide array of extracellular enzymes for nutrient recycling. Due to global warming, environmental pollution, green house gases emission and depleting fossil fuel, fungal enzymes for lignocellulosic biomass refinery become a major focus for utilizing renewal bioresources. Proteomic technologies tender better biological understanding and exposition of cellular mechanism of cell or microbes under particular physiological condition and are very useful in characterizing fungal secretome. Hence, in addition to traditional colorimetric enzyme assay, mass-spectrometry-based quantification methods for profiling lignocellulolytic enzymes have gained increasing popularity over the past five years. Majority of these methods include two dimensional gel electrophoresis coupled to mass spectrometry, differential stable isotope labeling and label free quantitation. Therefore, in this review, we reviewed more commonly used different proteomic techniques for profiling fungal secretome with a major focus on two dimensional gel electrophoresis, liquid chromatography-based quantitative mass spectrometry for global protein identification and quantification. We also discussed weaknesses and strengths of these methodologies for comprehensive identification and quantification of extracellular proteome.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2010.10a
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• pp.281-284
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• 2010

In this paper, we present Hand-gesture recognition for actual computing into color images from camera. Color images are binarization and labeling by using the YCbCr Color model. Respectively label area seeks the center point of the hand from to search Maximum Inscribed Circle which applies Voronoi-Diagram. This time, searched maximum circle and will analyze the elliptic ingredient which is contiguous so a hand territory will be able to extract. we present the presentation contral system using elliptic element and Maximum Inscribed Circle. This algorithm is to recognize the various environmental problems in the hand gesture recognition in the background objects with similar colors has the advantage that can be effectively eliminated.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2016.05a
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• pp.259-260
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• 2016

Recently, world-wide focusing on the interest for the reduction of greenhouse gas emissions associated with climate change and global warming, South Korea also has set up a national greenhouse gas reduction target and action plans seeking to achieve them. In particular, in the construction area, to encourage green building certification of the building and carbon labeling acquisition of building products, in order to reduce the environmental impact caused by the industrial activities have been in steady efforts. Therefore, this study estimates the life cycle carbon footprint of building construction materials applied to carbon emissions reduction technology and analyzes the results. Through the CO₂ emissions analysis in construction phase and maintenance phase of the building, it provides basic resource for future research expansion and establishes a step-by-step whole life cycle carbon emissions reduction plan in new construction and existing buildings.

• Journal of the East Asian Society of Dietary Life
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• v.22 no.2
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• pp.305-314
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• 2012

This study examined awareness and information acquisition behavior regarding food hygiene and safety and information search behavior of college students. The sample was obtained from 284 students in Yeungnam region through a self-administered questionnaire. To describe characteristics of the respondents, frequency distributions were used. In addition, $t$ tests, analysis of variance and Duncan multiple range tests were conducted. Data were analyzed by SPSS Windows V.18.0. The results of the study were as follows: (1) most respondents were concerned about food hygiene and safety. They were mostly interested in the expiration date label on breads, milk/dairy products, fish products and meat products, and price of cookies. Many respondents answered that foods produced and distributed in Korea were not safe. The respondents were most worried about endocrine disruptors among various food risk factors. The respondents received information on food hygiene and safety from blogs, mini-homepages, or Kin-searches on the Internet, electronic media, and food labeling in decreasing order.