• Genomics & Informatics
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• v.4 no.3
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• pp.129-132
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• 2006

Toxicogenomics has recently emerged in the field of toxicology and the DNA microarray technique has become common strategy for predictive toxicology which studies molecular mechanism caused by exposure of chemical or environmental stress. Although microarray experiment offers extensive genomic information to the researchers, yet high dimensional characteristic of the data often makes it hard to extract meaningful result. Therefore we developed toxicant enrichment analysis similar to the common enrichment approach. We also developed web-based system graPT to enable considerable prediction of toxic endpoints of experimental chemical.

• Genomics & Informatics
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• v.2 no.1
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• pp.53-56
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• 2004

• Journal of Microbiology and Biotechnology
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• v.12 no.6
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• pp.890-894
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• 2002

A xylanase-producing Trichoderma strain was isolated from soil. Xylanase from Trichoderma strain SY was purified 21-fold to an apparent homogeneity, with a $17.4\%$ yield. The optimum pH and temperature were determined to be 5.5 and $50^{\circ}C$, respectively, and its molecular weight was 21-kDa by SDS-PAGE. The corresponding gene, named xyl, was cloned by RT-PCR. DNA blot analysis of xyl showed that this gene is present as a single copy. The amino acid sequence of the Xyl protein showed similarity to those of other xylanases derived from various fungi. mRNA of xyl was highly expressed when this fungus was grown on cellulose or xylan as a sole carbon source, but undetectable when grown on sucrose. Extracts of Escherichia coli cells expressing xyl were found to have xylanase activity. It was confirmed that xyl from this isolate encodes xylanase.

• ALGAE
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• v.36 no.3
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• pp.165-174
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• 2021

Radicilingua Papenfuss and Calonitophyllum Aregood are two small genera of the family Delesseriaceae that consist of only three and one taxonomically accepted species, respectively. The type species of these genera, Radicilingua thysanorhizans from England and Calonitophyllum medium from the Americas, are morphologically very similar, with the only recognized differences being vein size and procarp development. To date, only other two species were recognized inside the genus Radicilingua: R. adriatica and R. reptans. In this study, we analysed specimens of Radicilingua collected in the Adriatic and Ionian Sea (Mediterranean), including a syntype locality of R. adriatica (Trieste, northern Adriatic Sea), alongside material from near the type locality of R. thysanorhizans (Torpoint, Cornwall, UK). The sequences of the rbcL-5P gene fragment here produced represent the first molecular data available for the genus Radicilingua. Phylogenetic reconstruction showed that the specimens from the Adriatic and Ionian Seas were genetically distinct from the Atlantic R. thysanorhizans, even if morphologically overlapping with this species. A detailed morphological description of the Mediterranean specimens, together with an accurate literature search, suggested that they were distinct also from R. adriatica and R. reptans. For these reasons, a new species was here described to encompass the Mediterranean specimens investigated in this study: R. mediterranea Wolf, Sciuto & Sfriso. Moreover, in the rbcL-5P tree, sequences of the genera Radicilingua and Calonitophyllum grouped in a well-supported clade, distinct from the other genera of the subfamily Nitophylloideae, leading us to propose that Calonitophyllum medium should be transferred to Radicilingua.

• Journal of Microbiology and Biotechnology
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• v.16 no.7
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• pp.1090-1096
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• 2006

O-Methylation is a common modification reaction found in nature, and is mediated by an O-methyltransferase (OMT). OMTs have been mainly studied in plants, whereas only a few OMTs have been studied in microbes. When searching the Bacillus cereus genome, four putative small molecular OMTs were identified, among which BcOMT-1 was cloned and expressed in E. coli as a his-tag fusion protein. The whole cell expressing BcOMT-1 was used to methylate several flavonoids. Eriodictyol, luteolin, quercetin, and taxifolin, all of which contain 3' and 4' hydroxyl groups, served as methyl group acceptors for BcOMT-1, whereas naringenin, apigenin, 3,3'-dihydroxyflavone, and 3,4'-dihydroxyflavone did not function as substrates. Analysis of the reaction products using HPLC showed two different peaks, and NMR revealed that the methylation position was at the hydroxyl group of either carbon 3' or 4'. Therefore, this showed that BcOMT-1 used flavonoids containing ortho hydroxyl groups and transferred a methyl group to either of two hydroxyl groups.

• Journal of Applied Biological Chemistry
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• v.48 no.3
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• pp.113-119
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• 2005

O-methylation mediated by O-methyltransferases (OMTs) is a common modification in natural product biosynthesis and contributes to diversity of secondary metabolites. OMTs use phenylpropanoids, flavonoids, other phenolics and alkaloids as substrates, and share common domains for S-adenosyl-L-methionine (AdoMet) and substrate binding. We searched Arabiposis genome and found 17 OMTs genes (AtOMTs). AdoMet- and substrate-binding sites were predicted. AdoMet binding domain of AtOMTs is highly conserved, while substrate-binding domain is diverse, indicating use of different substrates. In addition, expressions of six AtOMT genes in response to UV and in different tissues were investigated using real-time quantitative reverse transcriptase-polymerase chain reaction. All the AtOMTs investigated were expressed under normal growth condition and most, except AtOMT10, were induced after UV illumination. AtOMT1 and AtOMT8 were expressed in all the tissues, whereas AtOMT10 showed flower-specific expression. Analysis of these AtOMT gene expressions could provide some clues on AtOMT involvement in the cellular processes.

• The Journal of Korean Association of Computer Education
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• v.17 no.3
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• pp.41-51
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• 2014

Informatics gifted education institutions have made multi-faceted efforts to encourage students to do creative activities which can be extended lasting. However, research about gifted student's creative activities is yet incomplete, and most of the research has only focused on their cognitive abilities. Therefore, there is a limit to providing creative activity curricula that reflect the characteristics of informatics gifted students. This study assessed the creative products achievement of 56 informatics gifted elementary school students' and comprehensively analyzed the results with affective and environmental variables. The result is that the achievement of creative products was higher when the intrinsic motivation was found to be higher. Parents' support or extrinsic motivation did not differ significantly.

• Proceedings of the Korean Society of Toxicology Conference
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• 2005.11a
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• pp.47-75
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• 2005

• Journal of Fisheries and Marine Sciences Education
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• v.16 no.2
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• pp.227-237
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• 2004

Recently development trends in information technology expand the activity boundaries for human living beyond coastal zones. These rapid changes of paradigm are stimulating scientists and strategists to be encouraged in making adequate model for marine geographic information system (MGIS) and their applications. Coastal zones are places which are affected by many factors, such as water quality, tide, wave, atmosphere, population, industry, etc. Therefore information related to the coast has to be integrated and analyzed for managing it efficiently. GIS-based Ocean Informatics is suited for those purposes and used in the study for establishing and managing coastal geographic and environmental information system. This paper reviews the properties of ocean and coastal spaces, then defines some MGIS related terminologies for clarifying the scope of MGIS boundaries. In this study, coastal geographic information is established by digital geographic information and digital terrain information. Digital coast chart having information on digital chart and map can merge and analyze various coastal information and can be a useful tool for the coastal integrated management.

• Structural Engineering and Mechanics
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• v.12 no.2
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• pp.157-168
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• 2001

In this paper, experimental investigation into the behavior of reinforced concrete (RC) columns tested under large lateral displacement with four different types of loading arrangements is presented. Each loading arrangement has a different system for controlling the consistency of the loading condition. One of the loading arrangements used three units of link mechanism to control the parallelism of the top and bottom stub of column during testing, and the remaining employed eight hydraulic jacks for the same purpose. The loading systems condition used in this investigation were similar to the actual case in a moment-resisting frame where the tested column was displaced in a double curvature. Ten model column specimens, divided into four series were prepared. Two columns were tested monotonically until collapse, and unless failure took place at an earlier stage of loading, the remaining eight columns were tested under cyclic loading. Test results indicated that the proposed system to keep the top and bottom stubs parallel during testing performed well.