• Journal of Radiopharmaceuticals and Molecular Probes
• /
• v.9 no.1
• /
• pp.9-16
• /
• 2023

Liposomes as drug delivery system have proved useful carrier for various disease, including cancer. In addition, perfluorocarbon cored microbubbles are utilized in conjunction with high-intensity focused-ultrasound (HIFU) to enable simultaneous diagnosis and treatment. However, microbubbles generally exhibit lower drug loading efficiency, so the need for the development of a novel liposome-based drug delivery material that can efficiently load and deliver drugs to targeted areas via HIFU. This study aims to develop a liposome-based drug delivery material by introducing a substance that can burst liposomes using ultrasound energy and confirm the ability to target tumors using PET imaging. Liposomes (Lipo-DOX, Lipo-DOX-Au, Lipo-DOX-Au-RGD) were synthesized with gold nanoparticles using an avidin-biotin bond, and doxorubicin was mounted inside by pH gradient method. The size distribution was measured by DLS, and encapsulation efficiency of doxorubicin was analyzed by UV-vis spectrometer. The target specificity and cytotoxicity of liposomes were assessed in vitro by glioblastoma U87mg cells to HIFU treatment and analyzed using CCK-8 assay, and fluorescence microscopy at 6-hour intervals for up to 24 hours. For the in vivo study, U87mg model mouse were injected intravenously with 1.48 MBq of ⁶⁴Cu-labeled Lipo-DOX-Au and Lipo-DOX-Au-RGD, and PET images were taken at 0, 2, 4, 8, and 24 hours. As a result, the size of liposomes was 108.3 ± 5.0 nm at Lipo-DOX-Au and 94.1 ± 12.2 nm at Lipo-DOX-Au-RGD, and it was observed that doxorubicin was mounted inside the liposome up to 52%. After 6 hours of HIFU treatment, the viability of U87mg cells treated with Lipo-DOX-Au decreased by around 20% compared to Lipo-DOX, and Lipo-DOX-Au-RGD had a higher uptake rate than Lipo-DOX. In vivo study using PET images, it was confirmed that ⁶⁴Cu-Lipo-DOX-Au-RGD was taken up into the tumor immediately after injection and maintained for up to 4 hours. In this study, drugs released from liposomes-gold nanoparticles via ultrasound and RGD targeting were confirmed by non-invasive imaging. In cell-level experiments, HIFU treatment of gold nanoparticle-coupled liposomes significantly decreased tumor survival, while RGD-liposomes exhibited high tumor targeting and rapid release in vivo imaging. It is expected that the combination of these models with ultrasound is served as an effective drug delivery material with therapeutic outcomes.

• Journal of Marine Life Science
• /
• v.1 no.2
• /
• pp.95-108
• /
• 2016

This study was performed to investigate the community structure of macrobenthic assemblages in the Environmental Conservation area, Korea. Benthic animals were collected by van Veen grab sampler at spring (May) and summer (August) 2009. The total species number and mean density were 195 species 5.6 m^-2 and 667 individuals m^-2, respectively. Polychaetes were the most dominant faunal group in species (96 species) and abundance (431 individuals m^-2). The major dominant species were the polychaetes Lumbrineris longifolia (76±224 individuals m^-2), Mediomastus californiensis (42±117 individuals m^-2), Tharyx sp.3 (26±110 individuals m^-2), the bivalvia Theora fragilis (54±78 individuals m^-2) and the amphipod Eriopisella schellensis (70±146 individuals m^-2). Based on the cluster and nMDS ordination analysis, macrobenthic communities were divided into three faunal groups. The first group was characterized by high abundance of the polychaeta Sternaspis scutata and the amphipod Ampelisca cyclops iyoensis, which is located by most stations of Hampyeong Bay and St. 4 of Deungnyang Bay. The second group was numerically dominated by the polychaeta Capitella capitata at St. 4 and St. 5 in Gamak Bay where was most pollutant area. Finally, the third group was dominated by the polychaetes Heteromastus filiformis, Tharyx sp.3 and the amphipod Sinocorophium sinensis. Therefore, geochemical characteristics such as the bay shape and pollution gradient may be important factors controlling of the macrobenthic community structure in Environment Conservation Area.

• Journal of the Korean Society for Nondestructive Testing
• /
• v.22 no.4
• /
• pp.333-346
• /
• 2002

The continuous indentation techniques are one of the most effective methods to nondestructively estimate mechanical properties. There are many applications in various dimensions of materials from macro-scale, through micro-scale, even to nano-scale range. The macro-range technology of kgf-load level is now focused on the evaluation of tensile properties and residual stress of bulk materials, for example, used in conventional load-bearing structures and in-use pipelines. The technology and the apparatus were successfully developed by a domestic research group. The micro-range technology of gf-load level can be applied to investigate some property-gradient materials such as weldment. Because it has better spatial resolution than the macro-range technology. The nano-range technology (called nanoindentation technique) of mgf-load level is basically used to evaluate hardness and modulus of micro- and nano-materials. Moreover, many researches are going on to measure tensile properties and residual stress. The nanoindentation technology is easy to be applied to the various fields, such as semiconductor devices, multiphase materials, and biomaterials, though other methods are too difficult to be applied due to dimensional or environmental limitations. On the basis of these accomplishments, the international and the domestic standards are being established.

• Journal of Korean Society on Water Environment
• /
• v.28 no.1
• /
• pp.63-70
• /
• 2012

This study was carried out to investigate change in influent concentration of domestic wastewater flowed from a newly constructed separate sewer system (SSS) and biological nutrients removal efficiency of a full scale Air-vent sequential batch reactor (SBR, $600m^3/d$). The average concentration of $BOD_5$, SS, T-N and T-P from SSS were 246.5 mg/L, 231.6 mg/L, 42.974 mg/L, 5.360 mg/L, respectively which corresponds to 2.2times, 1.2times, 1.8times and 2.1times higher than those from the conventional combined sewer system (CSS). The removal efficiency of $BOD_5$, SS, T-N, and T-P for the Air-vent SBR operated with influent from SSS averaged 99.1%, 99.0%, 91.2%, and 93.5%, respectively. Especially the respective nitrogen and phosphorus removal was 15% greater than that of the SBR operated with influent from CSS. Simultaneous nitrification and denitrification (SND) was observed in an aerobic reactor(II) as a result of DO concentration gradient developed along the depth by the Air-vent system. In order to achieve T-N removal greater than 90%, the C/N ratio should be over 6.0 and the difference between $BOD_5$ loading and nitrogen loading rate be over 100 kg/day (0.130 kg $T-N/m^3{\cdot}d$). Even with high influent T-P concentration of 5.360 mg/L from SSS (compared with 2.465 mg/L from CSS) T-P removal achieved 93.5% which was 15.5% higher than that of the SBR with influent from CSS. This is probably due to high influent $BOD_5$ concentration from SSS that could provide soluble carbon source to release phosphorus at anaerobic condition. In order to achieve T-P removal greater than 90%, the difference between $BOD_5$ loading and phosphorus loading rate should be over 100 kg /day (0.130 kg $T-N/m^3{\cdot}d$).

• Journal of the Korean Wood Science and Technology
• /
• v.45 no.1
• /
• pp.107-115
• /
• 2017

In this study, the surface check reduction effect of preservative-treated Korean larch round-wood was investigated by applying three physical treatments, such as incising, kerfing, and kiln-drying. Moreover, the possibility of long-term service life was also checked by comparing the depth of surface check and the penetration depth of preservative. A rapid gradient of wood moisture content between surface and center causes a surface check. Thus, the effect of reducing surface check was carried out at the lowest equilibrium moisture content (EMC) condition, temperature ($2^{\circ}C$), humidity (44.6%), in outdoor locations in Korea until the test specimens reach to the 8.6% EMC. As a result, the preservative-treated specimens without incising, kerfing, kiln-drying (Type C) could not ensure the long-term service life due to a large surface check. Because the surface check depth of all specimens was deeper than the penetration depth of preservative. In case of the incising treated specimens (Type I), 80% of them, the depth of surface check was not deeper than the penetration depth of preservative. However, when a kerfing was additionally treated (Type B), a possibility of happening deeper surface check than penetration depth of preservative was increased rather than Type I.

• The Journal of Engineering Geology
• /
• v.21 no.3
• /
• pp.231-237
• /
• 2011

An active apatite drainage system has been developed at the Goro abandoned mine, comprising a grit cell, a reaction cell, and a precipitation pond. Leachate from an abandoned adit and tailing ponds is collected in a pipeline and is transported to the apatite drainage system under the influence of the hydraulic gradient. The results of a laboratory experiment performed in 2004 indicate that the reaction cell requires 38.8 ton/year of apatite and that precipitate will have to be removed from the precipitation pond every 3 months. The purpose of this study is to evaluate a laboratory test on the efficiency of limestone and apatite in removing arsenic from ARD (acid rock drainage), and to evaluate the suitability of materials for use as a precipitant for the leachate treatment disposal system. The laboratory tests show that the arsenic removal ratios of limestone and apatite are 67.4%-98.3%, and the arsenic removal ratio of apatite is inversely proportional to its grain size. The arsenic compounds are assumed to be Johnbaumnite and Ca-arsenic hydrate. Therefore, apatite and phosphorous limestone can be used as a precipitant for the removal of arsenic, although it is difficult to remove arsenic from ARD when it occurs in low concentrations.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.14 no.3
• /
• pp.403-414
• /
• 1994

Nonlinear analysis of RC containment structure under thermal load and pressure is presented to trace the behaviour after an assumed LOCA. The temperature distribution varying with time through the wall thickness is determined by transient finite element analysis with the two time level scheme in time domain. The layered shell finite elements are used to represent the containment structures in nuclear power plants. Both geometric and material nonlinearities are taken into account in the finite element formulation. The constitutive relation of concrete is modeled according to Drucker-Prager yield criteria in compression. Tension stiffening model is used to represent the tensile behaviour of concrete including bond effect. The reinforcing bars are modeled by smeared layer at the location of reinforcements accounting elasto-plastic axial behaviors. The steel liner model under Von Mises yield criteria is adopted to represent elastic-perfect plastic behaviour. Geometric nonlinearity is formulated to consider the large displacement effect. Thermal stress components are determined by the initial strain concept during each time step. The temperature differential between any two consecutive time steps is considered as a load incremental. The numerical results from this study reveal that nonlinear temperature gradient based on transient thermal analysis will produces excessive large displacement. Nonlinear behavior of containment structures up to ultimate stage can be traced reallistically. The present study allows more realistic analysis of concrete containment structures in nuclear power plants.

• Journal of the Korean Institute of Gas
• /
• v.15 no.3
• /
• pp.53-57
• /
• 2011

The present study has been carried out to analyze the flow characteristics of a heat recovery steam generator with the change of inlet flow conditions by using numerical flow analysis. The inlet of HRSG corresponds the outlet of gas turbine exit and the flow after gas turbine has strong swirl flow and turbulence. The inlet flow condition of HRSG should be included the exit flow characteristics of gas turbine. The present numerical analysis adopted the flow analysis result of gas turbine exit flow as a inlet flow condition of HRSG analysis. The computational flow analysis result of gas turbine exit shows that the maximum axial velocity appears near circular duct wall and the maximum turbulent kinetic energy and dissipation rate exist relatively higher gradient region of axial velocity. The comparison of flow analysis will be executed with change of inlet turbulent flow condition. The first case is using the inlet turbulent properties from the result of computational analysis of gas turbine exit flow, and the second case is using the assumed turbulent intensity with the magnitude proportional to the velocity magnitude and length scale. The computational results of flow characteristics for two cases show great difference especially in the velocity field and turbulent properties. The main conclusion of the present study is that the flow inlet condition of HRSG should be included the turbulent properties for the accurate computational result of flow analysis.

• Ocean and Polar Research
• /
• v.33 no.spc3
• /
• pp.349-358
• /
• 2011

In order to ascertain latitudinal variation of lipid contents and compositions in copepods, we collected warm water copepod species (Euchaeta sp. and Pleuromamma spp.) from four different regions from low (sub-tropical) to mid (temperate) latitudes in the Northwest Pacific Ocean. Total lipid contents of Pleuromamma spp. were about 11 $ug{\cdot}ind^{-1}$ with little latitudinal variation, whereas Euchaeta sp. showed slightly higher lipid content (20 $ug{\cdot}ind^{-1}$) than Pleuromamma spp. with latitudinal gradient (low at subtropic and high at temperate). Wax esters, known as the major storage lipid classes, were found to be the dominant lipid classes (accounting for more than 35% of total lipids) in Euchaeta sp., whereas in Pleuromamma spp., phospholipids, known as cellular membrane components, were the dominant lipid classes. However, the exception was specimens from warm pool region exhibiting dominance in storage of lipids as a form of triacylglycerols. Among fatty acids, polyunsaturated fatty acids (PUFA), especially docosahexaenoic acid (DHA : 22:6(n-3)) (about 35% of total fatty acids), were most abundant in Euchaeta sp., while saturated fatty acids (SAFA), specially hexadecanoic acid (16:0) (about 30% of total fatty acids), were most abundant in Pleuromamma spp.. Among the neutral fraction of lipids, phytol, originated from the side chain of chlorophyll, was found in all samples which generally indicate active copepods feeding on algae. While only trace amounts of short-chain fatty alcohols were found in Pleuromamma spp., significant amounts of fatty alcohols were found in Euchaeta sp.. Particularly, significant amounts of long chain monounsaturated fatty alcohols (20:1 and 22:1), generally found in cold water species, were found in Euchaeta sp. from low latitudes. The latitudinal variation of trophic lipid markers in these copepods could be significantly related with in-situ food availability and species-specific diet preference. The result of this study suggests that the lipid contents and compositions in copepods may not only indicate their nutritional condition and feeding ecology but also provide insight into species-specific living strategies under different environmental conditions (i.e. water temperature, food availability).

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.7
• /
• pp.275-282
• /
• 2018

Mountain trams are an environmental-friendly transportation system that run wirelessly on an embedded track constructed on previous mountain roads, and can run despite the frozen road. On the other hand, there is some difficulty on sharp and steep tracks. In this study, after possible technical problems were defined in mountain trams running on a sharp and steep track, the design solutions for the interface structure of bogie and carbody were proposed. In addition, a prototype was made and its performance was tested to verify the solutions. Because the difference in the distance of the inner and outer rails on a sharp curve is severe enough to interrupt running, independent rotating wheels with different angular speeds were developed and applied. To prevent derailment due to the large attack angle and lateral force caused by the previous vehicle of 2bogie-and-1carbody on the sharp curve, a vehicle with 1bogie-and-1carbody was designed and applied. A prototype vehicle of 1bogie-and-1carbody with independent rotating wheels was made to improve the performance during the test running on a small track. A coupler was designed to absorb the large rotations of 3 degrees-of-freedom between the carbodies of a mountain tram running on the steep curved track. After a small scale prototype was made, the performance was verified by a function test.