• 제목/요약/키워드: Environmental Research

검색결과 36,227건 처리시간 0.053초

ZGMMV와 CMV 동시 접종을 통한 바이러스 저항성 LM 식물의 잠재적 환경 위해성 연구 (Study on potential environmental risk of virus resistant LM plants using co-inoculation of Zucchini green mottle mosaic virus (ZGMMV) and Cucumber mosaic virus (CMV))

  • 송해룡;김태성;김선정;김용현;김기정;정현미;최희락;윤준헌
    • 환경영향평가
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    • 제22권2호
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    • pp.125-134
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    • 2013
  • Plant virus coat (CP) gene-mediated protection is one of the best known approaches to protect against virus resistant transgenic plants. Transgenic N. benthamiana plants containing the CP gene of Zucchini green mottle mosaic virus (ZGMMV) were used for the environmental risk assessment of the living modified (LM) plants with plant virus resistance. The most optimal co-infection method of both ZGMMV and CMV (Cucumber mosaic virus) on Non-LM and CP-expressing LM tobacco plants was established and co-infection of CMV and ZGMMV was confirmed by polymerase chain reaction (PCR). To address the effects of LM tobacco plants on the mutation of the virus, in-vitro transcripts of CP and Replicase (Rep) derived from CMV and/or ZGMMV were inoculated onto Non-LM or LM tobacco plants. Mutation frequency of CP and Rep from CMV and ZGMMV was examined through six serial passages in Non-LM and LM tobacco plants. Little actual frequency of mutation was estimated, probably due to the limited number of transgenic plants tested in this study. However, it does not suggest environmental safety of these CP-mediated LM plants. Further study at a larger scale is needed to evaluate the environmental risk associated with the CP-expressing LM plants.

생활화학제품 동시 사용현황 조사 - 방향제와 탈취제를 중심으로 - (An Investigation of Consumer Product Co-use Patterns - Focusing on air-fresheners and deodorizer -)

  • 이대엽;임현우;김주현;김탁수;황문영;석광설;서정관
    • 한국환경보건학회지
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    • 제44권3호
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    • pp.275-282
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    • 2018
  • Objective: Since certain chemicals are widely applied in a variety of consumer products, exposure via simultaneous use of multiple consumer products can put human health at risk. For this reason, use patterns of consumer products are an important factor inhuman health risk assessment. The aim of this study is to investigate co-use patterns of air-fresheners and deodorizers in Korea in order to estimate potential cumulative exposure. Methods: To collect the information on use patterns of air-fresheners and deodorizers, ten air-fresheners and 14 deodorizers were selected. A total of 3,000 participants were recruited and asked through on line questionnaires whether they had simultaneously used the selected air-fresheners and deodorant products in their daily lives. Results: Among the 3,000 participants, 45.6% had used more than two air fresheners and 46.3% used more than two deodorizers. The most common types of air fresheners used concurrently include liquid and candle types for indoor environments, while those of deodorizers were fabric trigger sprays and refrigerator gels. In addition, 73.7% used more than two products without product categories. Fabric trigger sprays were contained in all of the high-ranking product combinations. Conclusions: This study showed that many consumers concurrently used air-fresheners and deodorizers in their daily routines. For accurate exposure assessment, co-use patterns of consumer products should be further investigated.

여수 유류유출사고 방제작업자의 건강영향평가 (Health Effect Assessment on Cleanup Workers of an Oil Spill in Yeosu)

  • 김근배;강택신;윤미라;조혜정;주영경;유승도;이보은
    • 한국환경보건학회지
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    • 제42권6호
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    • pp.385-395
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    • 2016
  • Objectives: The aim of this study was to assess exposure to VOCs and PAHs and the health effects on volunteers who participated in an oil spill cleanup in Yeosu. Methods: Atmospheric VOCs were evaluated in the vicinity of the accident site and questionnaire surveys were conducted to identify personal characteristics and acute health symptoms of clean-up workers seven days after the accident. The levels of metabolites of VOCs (t,t-MA, HA, PGA, MA, MHA) and PAHs (2-NAP, 1-OHP, 2-HF, 1-HPH), oxidative stress markers (TABARS, 8-OHdG) in the urine of workers were analyzed. Their correlation was determined by multiple regression analysis with SAS ver. 9.4. Results: Although the concentration of atmospheric VOCs in the residential areas were low at the time of survey, the levels of VOCs and PAHs metabolites in clean-up workers were higher than those in the control group after clean-up activities. The levels of urinary VOC and PAH metabolites were significantly increased after clean-up compared to those measured before participation. The thiobarbituric acid reactive substance (TBARS) concentrations were also increased and showed significant correlations with those of metabolites of benzene. Conclusion: This study shows that oil spill clean-up activities affect exposure to VOCs and PAHs and the health of clean-up workers. The results suggest the need for check-ups of participants in oil spill cleaning work.

송사리 초기 생장단계에서의 비스페놀 A에 의한 내분비계장애 영향 (Endocrine Disrupting Effects of Bisphenol A on the Early Life - Stage of Medaka (Oryzias latipes))

  • 김은경;류지성;박수영;김현미;최광수;나진균;이철우
    • Environmental Analysis Health and Toxicology
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    • 제20권1호
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    • pp.13-21
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    • 2005
  • The purpose of this study was to determine the effects of bisphenol A (BPA), which is known to have estrogenic activity, on the early development of medaka fish (Oryzias latipes). The fertilized eggs of medaka were treated with BPA at different concentrations for 3 weeks. Embryonic growth, deformation, hatching success, and gonadal differentiation were determined to observe the effects of this chemical. Also we tried to measure the estrogenic activity of bisphenol A using ELISA and RT-PCR methods. By using this techniques, we evaluated the induction of vitellogenin, an estrogen-regulated gene from the whole body-homogenates of larvae. At results, a reduced blood circulation was seen in embryos and peritoneal edema and hindrance of yolk-sac absorption were observed in larvae of treated group. However, BPA at the concentrations tested (2~200 ㎍/L) did not have severe adverse effects on the early life-stages. According to the observation of gonadal histology, inter-sex or sex -reversal was not found in all test fish. After the exposure was ended, vitellogenin mRNA and protein levels were measured in larvae and then their levels were found to be increased in treated group with 200㎍/L. These results indicate that BPA can induce the expression of vitellogenin in early life-stages as well as in adult male fish.