• Title/Summary/Keyword: Environmental Research

Search Result 36,227, Processing Time 0.07 seconds

Variation of Lactate Dehydrogenase Isozymes in Angelfish (Pterophyllum scalare) according to Acute Environmental Change (급격한 환경변화에 대한 angelfish (Pterophyllum scalare) 젖산탈수소효소 동위효소의 변화)

  • An, Chang-Su;Cho, Sung-Kyu;Yum, Jung-Joo
    • Journal of Life Science
    • /
    • v.20 no.3
    • /
    • pp.416-423
    • /
    • 2010
  • In this study, the properties and gene expression of the lactate dehydrogenase (EC 1.1.1.27, LDH) isozyme were studied in angelfish (Pterophyllum scalare) - known for their adaptation to the low oxygen environment of the tropics - which were acclimated to acute temperature change ($27{\pm}0.5{\rightarrow}18{\pm}0.5^{\circ}C$) and dissolved oxygen (DO) change ($6{\pm}1{\rightarrow}18\;ppm$) for 2 hours. The properties of the LDH isozymes were confirmed in the native-polyacrylamide gel electrophoresis, Western blot analysis and enzyme activity measurement. Liver- and eye-specific Ldh-C gene were expressed in liver, eye and brain tissues. Through Western blot analysis, the LDH $A_4$ isozyme was shown to have a more cathodal mobility relative to the $B_4$ isozyme. In the liver tissue, the LDH $A_4$ isozyme increased with temperature drop while the $B_4$ isozyme decreased. The LDH $A_4$ and $C_4$ isozymes increased with DO increment, while the $B_4$ isozyme decreased. In the eye tissue, the LDH $A_4$ and B4 isozymse increased with temperature drop while the $B_4$ isozyme decreased. The LDH $A_4$ and $B_4$ isozymes increased with DO increment, but the $C_4$ isozyme and isozymes including the subunit C decreased. In the heart tissue, LDH activity increased with DO increment, as well as the LDH $B_4$ isozyme. In the brain tissue, the LDH $A_4$ and $B_4$ isozymes increased with temperature drop. The LDH $B_4$ isozyme increased with DO increment. Accordingly, since the liver- and eye-specific Ldh-C are influenced by changes in DO and the LDH $B_4$ and $C_4$ isozymes are relatively controlled in the liver and eye tissues, the $C_4$ isozyme can be considered to have a lactate oxidase function.

An Ecological Study on the Wetlands in Haman Area (함안지역 습지에 대한 생태학적 연구)

  • Cheong, Seon-Woo;Kim, In-Taek;Seo, Jeoung-Yoon;Park, Joong-Suk;Oh, Kyung-Hwan;Lee, Chan-Won
    • Journal of Wetlands Research
    • /
    • v.5 no.2
    • /
    • pp.15-32
    • /
    • 2003
  • The ecological study on seven wetlands of Haman area in Kyungsangnamdo, Korea, was carried out. In especial, the biological data of the sites were unknown. In this study, water quality including water temperature, pH, DO, COD, T-N, T-P, SS were tested. On the survey of plants and animals, vegetation and flora were investigated and the fauna of insects, fish, and amphibians were studied on each wetland. Water of wetland Oksu was heavily polluted and wetlands Pyungy and Dodulyangy were relatively clean. The water pollution was most severe in winter at all of the wetlands. Plant communities were classified into 9 natural communities and 1 artificial community. On the vegetation, wetland Sugok showed the highest plant taxa, and 41 families, 78 species and 16 varieties were classified. There was remarkable difference in number of plant taxa. The difference may be caused by the variances of wetland sizes, the influence from terrestrial environment. Wetland Sugok showed most rich insect fauna, and 10 orders, 76 families 224 species and 1082 individuals were identified. The species diversity was 2.05 and the species richness was 73.49. Wetland Ddun showed poor insect fauna, and 6 orders, 23 families, 29 species and 81 individuals were identified. Total collected fish were 4 orders, 7 families and 11 species. The fish fauna was most rich in wetlands Oksu and Pyungy, but poor in wetland Unan. Total collected amphibians were 2 orders, 3 families and 4 species.

  • PDF

Genotypic and Phenotypic Characteristics of Staphylococcus aureus Isolates from Lettuces and Raw Milk (상추와 원유에서 분리한 황색 포도상구균의 유전형 및 표현형 특징)

  • Jung, Hye-Jin;Cho, Joon-Il;Park, Sung-Hee;Ha, Sang-Do;Lee, Kyu-Ho;Kim, Cheol-Ho;Song, Eun-Seop;Chung, Duck-Hwa;Kim, Min-Gon;Kim, Kwang-Yup;Kim, Keun-Sung
    • Korean Journal of Food Science and Technology
    • /
    • v.37 no.1
    • /
    • pp.134-141
    • /
    • 2005
  • To characterize genotypic and phenotypic traits of Staphylococcus aureus isolates (n = 86) from lettuces and raw milk, major virulence-associated genes and antibiotic susceptibility were detected using PCR-based methods and disk diffusion method, respectively. All isolates possessed coagulase gene and showed five polymorphism types [500 bp (2.4%), 580 bp (17.4%), 660 bp (61.6%), 740 bp (17.4%), and 820 bp (1.2%)] due to variable numbers of tandem repeats present within the gene. Two or three different loci of hemolysin gene family were dominant in isolates, 47 of which (55%) possessed combination of hla/hld/hlg-2 genes as the most prevalent types. Among enterotoxin-encoding genes, sea was detected from 32 isolates (37%), sed from 1 isolate (1%), and sea and sed genes were co-detected from 4 isolates (5%), whereas seb, sec, and tsst-1 genes were not detected. All isolates were susceptible to ciprofloxacin, trimethoprim/sulfamethoxazole, oxacillin, and vancomycin, 85 isolates (99%) to penicillin G, 54 isolates (63%) to chloramphenicol, 51 isolates (59%) to erythromycin, and 7 isolates (8%) to clindamycin. Among resistant isolates, seven displayed multiantibiotic-resistance against two different antibiotics.

Classification of Seismic Stations Based on the Simultaneous Inversion Result of the Ground-motion Model Parameters (지진동모델 파라미터 동시역산을 이용한 지진관측소 분류)

  • Yun, Kwan-Hee;Suh, Jung-Hee
    • Geophysics and Geophysical Exploration
    • /
    • v.10 no.3
    • /
    • pp.183-190
    • /
    • 2007
  • The site effects of seismic stations were evaluated by conducting a simultaneous inversion of the stochastic point-source ground-motion model (STGM model; Boore, 2003) parameters based on the accumulated dataset of horizontal shear-wave Fourier spectra. A model parameter $K_0$ and frequency-dependent site amplification function A(f) were used to express the site effects. Once after a H/V ratio of the Fourier spectra was used as an initial estimate of A(f) for the inversion, the final A(f) which is considered to be the result of combined effect of the crustal amplification and loca lsite effects was calculated by averaging the log residuals at the site from the inversion and adding the mean log residual to the H/V ratio. The seismic stations were classified into five classes according to $logA_{1-10}^{max}$(f), the maximum level of the site amplification function in the range of 1 Hz < f < 10 Hz, i.e., A: $logA_{1-10}^{max}$(f) < 0.2, B: 0.2 $\leq$ $logA_{1-10}^{max}$(f) < 0.4, C: 0.4 $\leq$ $logA_{1-10}^{max}$(f) < 0.6, D: 0.6 $\leq$ $logA_{1-10}^{max}$(f) < 0.8, E: 0.8 $\leq$ $logA_{1-10}^{max}$(f). Implication of the classified result was supported by observing a shift of the dominant frequency of average A(f) for each classified stations as the class changes. Change of site classes after moving seismic stations to a better site condition was successfully described by the result of the station classification. In addition, the observed PGA (Peak Ground Acceleration)-values for two recent moderate earthquakes were well classified according to the proposed station classes.

Numerical Test for the 2D Q Tomography Inversion Based on the Stochastic Ground-motion Model (추계학적 지진동모델에 기반한 2D Q 토모그래피 수치모델 역산)

  • Yun, Kwan-Hee;Suh, Jung-Hee
    • Geophysics and Geophysical Exploration
    • /
    • v.10 no.3
    • /
    • pp.191-202
    • /
    • 2007
  • To identify the detailed attenuation structure in the southern Korean Peninsula, a numerical test was conducted for the Q tomography inversion to be applied to the accumulated dataset until 2005. In particular, the stochastic pointsource ground-motion model (STGM model; Boore, 2003) was adopted for the 2D Q tomography inversion for direct application to simulating the strong ground-motion. Simultaneous inversion of the STGM model parameters with a regional single Q model was performed to evaluate the source and site effects which were necessary to generate an artificial dataset for the numerical test. The artificial dataset consists of simulated Fourier spectra that resemble the real data in the magnitude-distance-frequency-error distribution except replacement of the regional single Q model with a checkerboard type of high and low values of laterally varying Q models. The total number of Q blocks used for the checkerboard test was 75 (grid size of $35{\times}44km^2$ for Q blocks); Q functional form of $Q_0f^{\eta}$ ($Q_0$=100 or 500, 0.0 < ${\eta}$ < 1.0) was assigned to each Q block for the checkerboard test. The checkerboard test has been implemented in three steps. At the first step, the initial values of Q-values for 75 blocks were estimated. At the second step, the site amplification function was estimated by using the initial guess of A(f) which is the mean site amplification functions (Yun and Suh, 2007) for the site class. The last step is to invert the tomographic Q-values of 75 blocks based on the results of the first and second steps. As a result of the checkerboard test, it was demonstrated that Q-values could be robustly estimated by using the 2D Q tomography inversion method even in the presence of perturbed source and site effects from the true input model.

Actual Vegetation and Structure of Plant Community in Daegwallyeong Ranch, Gangwon-do (Province) (강원도 대관령 목장 현존식생 및 식물군집구조)

  • Noh, Tai-Hwan;Han, Bong-Ho;Kim, Jong-Yup;Lee, Min-Young;Yoo, Ki-Joon
    • Korean Journal of Environment and Ecology
    • /
    • v.27 no.5
    • /
    • pp.579-591
    • /
    • 2013
  • This study was carried out to investigate the actual vegetation, the structure of plant community, and ecological succession sere of forest ecosystem in temperate northern climate zone, Daegwallyeong Ranch, Gangwon-do (Province) and to offer the basic data for planning of the forest managemant. As a result of analysis of actual vegetation, vegetation types divided into 56types and the area of survey site was $19,397,361m^2$. The ratio of vegetation type dominated by Quercus mongolica forest was 39.1%, primary grassland was 24.7%, Quercus mongolica-Deciduous broad-leaved forest was 11.3%. Twenty eight plots (size is $20m{\times}20m$) were set up and the results analyzed by DCA which in one of the ordination technique showed that the plant communities were divided into six groups which area community I (Pinus densiflora-Quercus mongolica community), community II (Quercus mongolica-Pinus densiflora community), community III (Quercus mongolica community), community IV (Quercus mongolica-Deciduous broad-leaved community), community V (Deciduous broad-leaved community), community VI (Sorbus alnifolia community). The age of community Iwas ranged from 57 to 62 years old, that of community IIwas ranged from 41 to 77 years old, community III was ranged from 47 to 108 years old, community IV was ranged from 47 to 82 years old, community V was 47 years old, community VI was 55 years old, thus we supposed that the age of the study site is about from 41 to 108 years old. The Ecological succession is predicted from Pinus densiflora community to Quercus mongolica community and Deciduous broad-leaved were distributed in the center of the valley in Daegwallyeong Ranch. According to the index of Shannon's diversity (unit: $400m^2$), community IV was ranged from 0.8203 to 1.1439, community III was ranged from 0.8019 to 1.1375, community V was 1.0993, community I was ranged from 0.9475 to 1.0797, community II was ranged from 0.6896 to 1.0324, community VI was 0.9909.

Health Condition Assessment Using the Riparian Vegetation Index and Vegetation Analysis of Geumgang mainstream and Mihocheon (수변식생지수를 이용한 금강본류와 미호천의 건강성 평가 및 식생분석)

  • Lee, Seung-Yeon;Jang, Rae-Ha;Han, Young-Sub;Jung, Young-Ho;Lee, Soo-In;Lee, Eung-Pill;You, Young-Han
    • Korean Journal of Environment and Ecology
    • /
    • v.32 no.1
    • /
    • pp.105-117
    • /
    • 2018
  • This study conducted health assessment and multivariate vegetation analysis using the riparian vegetation index in 30 sites of the Geumgang mainstream and Mihocheon to obtain practical data on the river management of the Geumgang. The result showed that the number of plant communities was 54. The flora was 75 families, 185 genera, 243 species, 2 subspecies, 21 varieties, 2 varieties, and 268 taxa. The riparian vegetation index was 38.3 (3.3; G-D1 ~ 66.7; G-U2, G-U4, and G-M3), and the health of the rivers in this area was evaluated as normal (grade C). The health of rivers was the highest in the upper stream of Geumgang mainstream and lowest in the downstream of Geumgang mainstream. The relationship between riparian vegetation index and chlorophyll-a content was low. The riparian vegetation was divided into five groups of Digitaria ciliaris colony group, Salix gracilistyla colony group, Erigeron annuus colony group, the group dominated by Humulus japonicus, Salix koreensis, Miscanthus sacchariflorus, and Phragmites japonica colonies, and the group dominated by Conyza canadensis and Echinochloa crusgalli var. echinata colonies. They had the similar health conditions. The CCA analysis showed that the environmental factors affecting the distribution of vegetation were physical factors such as vegetation area, artificial structure area, waterway area, branch width, channel width, and bank height and the biological factors such as the number of species. As such, it is necessary to maintain the health condition through continuous monitoring where the health condition is high and to apply active measures such as ecological restoration where the health condition is low.

Crime Incident Prediction Model based on Bayesian Probability (베이지안 확률 기반 범죄위험지역 예측 모델 개발)

  • HEO, Sun-Young;KIM, Ju-Young;MOON, Tae-Heon
    • Journal of the Korean Association of Geographic Information Studies
    • /
    • v.20 no.4
    • /
    • pp.89-101
    • /
    • 2017
  • Crime occurs differently based on not only place locations and building uses but also the characteristics of the people who use the place and the spatial structures of the buildings and locations. Therefore, if spatial big data, which contain spatial and regional properties, can be utilized, proper crime prevention measures can be enacted. Recently, with the advent of big data and the revolutionary intelligent information era, predictive policing has emerged as a new paradigm for police activities. Based on 7420 actual crime incidents occurring over three years in a typical provincial city, "J city," this study identified the areas in which crimes occurred and predicted risky areas. Spatial regression analysis was performed using spatial big data about only physical and environmental variables. Based on the results, using the street width, average number of building floors, building coverage ratio, the type of use of the first floor (Type II neighborhood living facility, commercial facility, pleasure use, or residential use), this study established a Crime Incident Prediction Model (CIPM) based on Bayesian probability theory. As a result, it was found that the model was suitable for crime prediction because the overlap analysis with the actual crime areas and the receiver operating characteristic curve (Roc curve), which evaluated the accuracy of the model, showed an area under the curve (AUC) value of 0.8. It was also found that a block where the commercial and entertainment facilities were concentrated, a block where the number of building floors is high, and a block where the commercial, entertainment, residential facilities are mixed are high-risk areas. This study provides a meaningful step forward to the development of a crime prediction model, unlike previous studies that explored the spatial distribution of crime and the factors influencing crime occurrence.

Experimental Study on Structural Behavior of Precast PSC Curved Girder Bridge (프리캐스트 PSC 곡선 거더교의 구조거동에 대한 실험적 연구)

  • Kim, Sung Jae;Kim, Sung Bae;Uhm, Ki Ha;Kim, Jang Ho Jay
    • KSCE Journal of Civil and Environmental Engineering Research
    • /
    • v.34 no.6
    • /
    • pp.1731-1741
    • /
    • 2014
  • Recently, many overpasses, highway, and advanced transit systems have been constructed to distribute the traffic congestion, thus small size of curved bridges with small curvature such as ramp structures have been increasing. Many of early curved bridges had been constructed by using straight beams with curved slabs, but curved steel beams have replaced them due to the cost, aesthetic and the advantage in building the section form and manipulating the curvature of beams, thereby large portion of curved bridges were applied with steel box girders. However, steel box girder bridges needs comparatively high initial costs and continuous maintenance such as repainting, which is the one of the reason for increasing the cost. Moreover, I-type steel plate girder which is being studied by many researchers recently, seem to have problems in stability due to the low torsional stiffness, resulting from the section characteristics with thin plate used for web and open section forms. Therefore, in recent studies, researchers have proposed curved precast PSC girders with low cost and could secured safety which could replace the curved steel girder type bridges. Hence, this study developed a Smart Mold system to manufacture efficient curved precast PSC girders. And by using this mold system a 40 m 2-girder bridge was constructed for a static flexural test, to evaluate the safety and performance under ultimate load. At the manufacturing stage, each single girder showed problems in the stability due to the torsional moment, but after the girders were connected by cross beams and decks, the bridge successfully distributed the stress, thereby the stability was confirmed. The static loading test results show that the initial crack was observed at 1,400 kN when the design load was 450 kN, and the load at the allowable deflection by code was 1,800 kN, which shows that the safety and usability of the curved precast PSC bridge manufactured by Smart Mold system is secured.

The Strength Properties Activated Granulated Ground Blast Furnace Slag with Aluminum Potassium Sulfate and Sodium Hydroxide (칼륨명반과 수산화나트륨으로 활성화된 고로슬래그 미분말의 강도 특성)

  • Kim, Taw-Wan;Hahm, Hyung-Gil
    • Journal of the Korea Concrete Institute
    • /
    • v.27 no.2
    • /
    • pp.95-102
    • /
    • 2015
  • In this paper, the effects of sodium hydroxide (NaOH) and aluminum potassium sulfate ($AlK(SO_4)_2{\cdot}12H_2O$) dosage on strength properties were investigated. For evaluating the property related to the dosage of alkali activator, sodium hydroxide (NaOH) of 4% (N1 series) and 8% (N2 series) was added to 1~5% (K1~K5) dosage of aluminum potassium sulfate ($AlK(SO_4)_2{\cdot}12H_2O$) and 1% (C1) and 2% (C2) dosage of calcium oxide (CaO). W/B ratio was 0.5 and binder/ fine aggregate ratio was 0.5, respectively. Test result clearly showed that the compressive strength development of alkali-activated slag cement (AASC) mortars were significantly dependent on the dosage of NaOH and $AlK(SO_4)_2{\cdot}12H_2O$. The result of XRD analysis indicated that the main hydration product of $NaOH+AlK (SO_4)_2{\cdot}12H_2O$ activated slag was ettringite and CSH. But at early ages, ettringite and sulfate coated the surface of unhydrated slag grains and inhibited the hydration reaction of slag in high dosage of $NaOH+AlK(SO_4)_2{\cdot}12H_2O$. The $SO_4{^{-2}}$ ions from $AlK(SO_4)_2{\cdot}12H_2O$ reacts with CaO in blast furnace slag or added CaO to form gypsum ($CaSO_4{\cdot}2H_2O$), which reacts with CaO and $Al_2O_3$ to from ettringite in $NaOH+AlK(SO_4)_2{\cdot}12H_2O$ activated slag cement system. Therefore, blast furnace slag can be activated by $NaOH+AlK(SO_4)_2{\cdot}12H_2O$.