• Korean Journal of Ichthyology
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• v.33 no.4
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• pp.226-239
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• 2021

Sebastes minor, Sebastes trivittatus, Sebastes owstoni, and Sebastes steindachneri are indigenous fish species inhabiting the central part of the East Sea, Korea. In order to understand the molecular evolution of these four rockfishes, we sequenced the complete mitochondrial genomes (mitogenomes) of S. minor and S. trivittatus. To further analyze the phylogeny of Sebastes species, the mitogenomes of 16 rockfishes were comparatively investigated. The complete mitochondrial DNA (mtDNA) nucleotide sequences of S. minor and S. trivittatus were 16,408 bp and 16,409 bp in length, respectively. A total of 37 genes were found in mtDNA of S. minor and S. trivittatus, including 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes, which exhibited similar characters with other Sebastes species in the East Sea, Korea. In addition, we detected a conserved motif "ATGTA" in the control region of the four Sebastes species, but no tandem repeat units. Comparative analyses of the congeneric mitochondrial genomes were performed, which showed that control regions were more variable than the concatenated protein-coding genes. As a result of analysing phylogenetic relationships of four Sebastes species by using concatenated nucleotide sequences of 13 protein-coding genes, S. minor, S. trivittatus, S. owstoni and S. steindachneri were clustered into three clades. The phylogenetic tree exhibited that S. minor and S. steindachneri shared a closer relationship, whereas S. trivittatus and S. vulpes formed another distinct clade. Our results contribute to a better understanding of evolutionary patterns of Sebastes species inhabiting the middle East Sea, Korea.

• Journal of Korean Society of Forest Science
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• v.112 no.1
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• pp.40-56
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• 2023

Tree species within the Populus genus grow rapidly and have an excellent capacity to absorb carbon, conferring substantial ability to effective purify the environment. Poplar breeding can be achieved rapidly and efficiently if a genetic linkage map is constructed and quantitative trait loci (QTLs) are identified. Here, a high-density genetic linkage map was constructed for the control pollinated progeny using the genotyping-by-sequencing (GBS) technique, which is a next-generation sequencing method. A search was also performed for the genes associated with quantitative traits located in the genetic linkage map by examining the variables of height and diameter at root collar, and resilience to insect damage. The height and diameter at root collar were measured directly, while the ability to recover from insect damage was scored in a 4-year-old breeding population of aspen hybrids (Odae19 × Bonghyeon4 F1) established in the research forest of Seoul National University. After DNA extraction, paternity was confirmed using five microsatellite markers, and only the individuals for which paternity was confirmed were used for the analysis. The DNA was cut using restriction enzymes and the obtained DNA fragments were prepared using a GBS library and sequenced. The analyzed results were sorted using Populus trichocarpa as a reference genome. Overall, 58,040 aligned single-nucleotide polymorphism (SNP) markers were identified, 17,755 of which were used for mapping genetic linkages. The genetic linkage map was divided into 19 linkage groups, with a total length of 2,129.54 cM. The analysis failed to identify any growth-related QTLs, but a gene assumed to be related to recovery from insect damage was identified on linkage group (chromosome) 4 through genome-wide association study.

• Korean Journal of Ecology and Environment
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• v.35 no.1 s.97
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• pp.52-61
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• 2002

This research was conducted to evaluate the effect of forest management practices on pH and electrical conductivity to get fundamental information on water purification capacity after forest operation. Rainfall, throughfall and stemflow　were　sampled at the study sites which consist of Abies holophylla and Pinus koraiensis　in Gwangreung Experimental Forest for S months from May to November 1999. Mean pH of the throughfall of the beginning of the event was higher in management (thinning and pruning) sites of Abies holophylla and Pinus koraiensis stands than nonmanagement site of Abies holophylla and Pinus koraiensis stands. In addition, pH of the throughfall of the total amount of the event showed similar trends which are higher pH in the management sites compared with the non- management sites. This result indicates that managements such as thinning and pruning improve tree butler capacity of rainfall pH. According to the linear regression results, pH of the throughfall of the total amount of the event in non-management sites = 0.735${\times}$pH of the throughfall of the beginning of the event in non-management sites+1.849 ($R^2\;=\;0.82$) and pH of the throughfall of the total amount of the event in management sites= 0.863${\times}$pH of the throughfall of the beginning of the event in management sites +1.0242 ($R^2\;=\;0.87$). In case of stemflow pH, pH of the sternflow of the total amount of　the event in non-management sites = 0.53${\times}$pH of the stemflow of the beginning of　the event in non- management sites+2.7709 ($R^2\;=\;0.64$) and pH of the stemflow of the total amount of the event in management sites = 0.5854${\times}$pH of the stemflow of the beginning of the event in management sites+2.7045 ($R^2\;=\;0.65$). Electrical conductivity (EC) of the throughfall of the beginning and total amount of the event was highest in non- management site in Abies holophylla, followed by management sites in fsies Abies holophylla, non-management site in Pinus koraiensis, and management sites in Pinus koraiensis stands, respectively. According to the linear regression results, EC of the throughfall of the total amount of the event in non-managementsites = 0.4045${\times}$EC of the throughfall of the beginning of the event in non-management sites+26.766 ($R^2\;=\;0.69$) and EC of the throughfall of the total amount of the event in management sites = 0.6002${\times}$EC of the throughfall of the beginning of the event in management sites+8.0184 ($R^2\;=\;0.54$). In case of stemflow EC, EC of thestemflow of the total amount of the event in non-management sites = 0.6298${\times}$EC of the stemflow of the beginning of the event in non-management sites+11.582 ($R^2\;=\;0.72$) and pH of the stemflow of the total amount of the event in management sites =0.602${\times}$pH of the stemflow of the beginning of the event in management sites+20.783($R^2\;=\;0.49$).

• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.1
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• pp.1-9
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• 2006

Regardless of the recent observed warmer winters in Korea, more freeze injuries and associated economic losses are reported in fruit industry than ever before. Existing freeze-frost forecasting systems employ only daily minimum temperature for judging the potential damage on dormant flowering buds but cannot accommodate potential biological responses such as short-term acclimation of plants to severe weather episodes as well as annual variation in climate. We introduce 'dormancy depth', in addition to daily minimum temperature, as a complementary criterion for judging the potential damage of freezing temperatures on dormant flowering buds of grape vines. Dormancy depth can be estimated by a phonology model driven by daily maximum and minimum temperature and is expected to make a reasonable proxy for physiological tolerance of buds to low temperature. Dormancy depth at a selected site was estimated for a climatological normal year by this model, and we found a close similarity in time course change pattern between the estimated dormancy depth and the known cold tolerance of fruit trees. Inter-annual and spatial variation in dormancy depth were identified by this method, showing the feasibility of using dormancy depth as a proxy indicator for tolerance to low temperature during the winter season. The model was applied to 10 vineyards which were recently damaged by a cold spell, and a temperature-dormancy depth-freeze injury relationship was formulated into an exponential-saturation model which can be used for judging freeze risk under a given set of temperature and dormancy depth. Based on this model and the expected lowest temperature with a 10-year recurrence interval, a freeze risk probability map was produced for Hwaseong County, Korea. The results seemed to explain why the vineyards in the warmer part of Hwaseong County have been hit by more freeBe damage than those in the cooler part of the county. A dormancy depth-minimum temperature dual engine freeze warning system was designed for vineyards in major production counties in Korea by combining the site-specific dormancy depth and minimum temperature forecasts with the freeze risk model. In this system, daily accumulation of thermal time since last fall leads to the dormancy state (depth) for today. The regional minimum temperature forecast for tomorrow by the Korea Meteorological Administration is converted to the site specific forecast at a 30m resolution. These data are input to the freeze risk model and the percent damage probability is calculated for each grid cell and mapped for the entire county. Similar approaches may be used to develop freeze warning systems for other deciduous fruit trees.