• Weed & Turfgrass Science
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• v.4 no.3
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• pp.159-175
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• 2015

Surveys of weed species on upland fields were conducted in Korea to investigate the occurrence of weed flora from April to May 2014 for winter crop fields and from July to August 2014 for summer crop fields. From the nation-wide survey, 375 weed species in 50 families were identified and classified to 162 annuals, 78 biennials and 135 perennials. Based on the occurrence ratio, the most weed species belonged to Compositae (73 species). 44 and 25 weed species belonged to Poaceae and Polygonaceae, respectively, and these 183 weed species in the most five families accounted for 49% of total weed occurrence. While 287 weed species in 45 families occurred in the winter crop fields, 339 weed species in 47 families occurred in summer crop fields. The most dominant weed species in Korean upland fields were Digitaria ciliaris, followed by Portulaca oleracea, Acalypha australis, Chenopodium album, Rorippa palustris etc. 129 weed species in 25 families were considered as exotic weeds. Based on the importance analysis, the highest value was C. album followed by Amaranthus lividus, Conyza canadensis etc. This information could be useful for estimation of future weed occurrence, weed population dynamics and establishment of weed control methods in upland fields of Korea.

• Korean Journal of Ecology and Environment
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• v.41 no.spc
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• pp.77-85
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• 2008

Grazing rates (GR) and pseudofaeces production (PFP) of native snail, Chinese mystery snail (Cipangopaludina chinensis malleata Reeve) on natural colonial morphs of Microcystis aeruginosa was measured. C. chinensis was collected from the upstream of the Geum River (Boryeong, Korea), where they co-habituated with Unio douglasiae and Lanceolaria acrorhyncha. The experiments were performed to evaluate the GR and PFP at different conditions such as; incubation time (1, 3, 5, 7, 9 and 11 hr), body size (3 to 6.1 cm, n=28), snail density (0.5, 1, 1.5 and 2.0 ind. $L^{-1}$) and prey concentration (168.3, 336.7, 505.0 and $673.0{\mu}g\;Chl-{\alpha}L^{-1}$). All experiments were triplicated, and conducted in transparent acrylic vessel (3L in volume). Regarding feeding time, a highest GR (0.538L $gAFDW^{-1}h^{-1}$) and PFP $(7.18mgAFDW^{-1})$ appeared at 1hr and 7hr after snail stocking, respectively. Interestingly, the snail, smaller than 4.5cm in body size, showed a wide range of GR ($-4.173{\sim}1.087L\;gAFDW^{-1}h^{-1}$) for the initial period (1 and 4hrs of stocking), compared to those greater than 4.5cm, which showed a stable FR, higher than 0.5L $gAFDW^{-1}h^{-1}$. Upon density effect, the density of 1.5 ind. $L^{-1}$ induced the most effective inhibition on Microcystis biomass with highest PFP. On the prey concentration, highest GR (0.897L $gAFDW^{-1}h^{-1}$) and PFP (3.67 mg $gAFDW^{-1}h^{-1}$) were induced at the level of $168.3{\mu}g\;Chl-{\alpha}L^{-1}$ and $673{\mu}g\;Chl-{\alpha}L^{-1}$, respectively. GR and PFP of this freshwater snail on the cyanobacterial bloom (M. aeruginosa) varied with the feeding conditions, and they were comparatively high for a short period of time less than 7hrs regardless of the stocking condition. Our results suggest that this freshwater snail has a potential to control cyanobacterial bloom when provided with suitable condition.

• Korean Journal of Ecology and Environment
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• v.41 no.spc
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• pp.68-76
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• 2008

This study examined the inhibition effects of a freshwater bivalve (Unio douglasiae) and a submerged plant (Potamogeton crispus) on the cyanobacterial bloom (Oscillatoria sp.). The experiment were conducted in aquarium $(50cm{\times}65cm{\times}120cm)$ with lake sediments in the bottom of the aquarium in 10 cm thick. Before the experiments, artificial cyanobacterial bloom was induced with the addition of lake sediment and CB medium. Total 12 transparent acrylic cylinders (${\Phi}19cm$, height 40 cm) were placed in the aquarium, and within which bivalves and plants were placed in various conditions such as the control (C), plant addition (P:5 stems), mussel addition (U:2 individuals), and both mussel and plant addition (PU: the same quantity as used in each treatment). The experiment was conducted in triplicate during 7 days. pH, dissolved oxygen (DO), electric conductivity (EC), salinity, cyanobacterial cell density, chlorophyll-${\alpha}$ concentration, and mussel filtering rate were monitored daily. At the end of the experiment, total phosphorus (TP), total nitrogen (TN), and plant height and weight were measured. Overall, a large degree of cyanobacterial growth inhibition appeared in both P and U treatments, and the effect was highest in the U treatment, followed by P and PU. The combined treatment of both U and P did not show any synergic effects compared to the effect in separated treatment. In all enclosures of the treatments chlorophyll-${alpha}$ (Chl-${alpha}$) concentration decreased until 36 hours after the additions of the plants and mussels. In contrast, Chl-${alpha}$ concentrations increased in PU enclosures after 36 hours. The same trend was shown in the cell density of Oscillatoria. pH and DO gradually decreased until 120 and 144 hours, respectively, in the P and PU enclosures. TP concentration increased in the mussel enclosures (U and PU), while TN concentration largely decreased in the plant enclosures (P and PU). Our results suggest that applied bivalve (Unio) and submerged plant (Potamogeton) seemed to have a potential effect on the growth inhibition of cyanobacteria, but their combined application may have an antagonistic effect to diminish the degree of the inhibition.

• Korean Journal of Ecology and Environment
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• v.41 no.spc
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• pp.35-41
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• 2008

Nutrient Enrichment Bioassays (NEBs) were conducted in the laboratory during June $22{\sim}28$, 2006 in order to determine primary limiting factor on the phytoplankton growth. For the NEBs, the water was sampled using a 10L polyethylene-lined container and dispensed into 2.5L container in the laboratory. The algal growths response in the control (C) and three treatments of phosphorus (P), 2-fold phosphorus (2P), and nitrate nitrogen $(NO_3-N)$ were monitored during 7 days. In the cubitainers which were spiked as P (T1) and 2P (T2) Chl-${\alpha}$ concentrations were decreased during the test period and the final concentrations was low than initial values. However, Chl-${\alpha}$ in the cubitainers which were spiked as $NO_3$(T3) and $P+NO_3$(T4) showed significant increases compared to the initial values, indicating that in the short-term experiments, nitrogen seemed to be a primary limiting nutrient during the periods of NEBs experiment. Long-term ambient nutrient data of TP and TN, and TN:TP mass ratios, however, showed a potential phosphorus limitation on phytoplankton growth and previous other researchers showed a variations of limiting nutrients by nitrogen or phosphorus depending on the seasons sampled and locations. In this study nitrogen as primary limiting nutrient in the NEBs seem to be an seasonal effect rather than the consistent nitrogen limitation.

• Korean Journal of Ecology and Environment
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• v.41 no.spc
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• pp.50-60
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• 2008

We performed the experiment to evaluate the effect of different DO concentrations (0.5, 4.5 and 9.0 $mgO_2L^{-1}$) and water depths (20, 50 and 80 cm) on the filtering rate, mortality, and pseudifeces production of Unio douglasiae against the cyanobacterial bloom (mainly Microcystis aeruginosa). A solitary-living bivalve U. douglasiae was collected in the upstream region of the North Han River (Korea). The harvested mussels were carefully transferred to the laboratory artificial management system, which was controlled temperature $(18{\pm}2^{\circ}C)$, flow rate (10L $h^{-1}$), food $(Chlorella^{TM})$, sediment (pebble and clay), light intensity (ca. $20{\mu}mol$ photons), and photocycle (12 L : 12 D). In the field observation, the mussel mortality was significantly correlated with water temperature, pH and DO concentration (P<0.05). The mortality was decreased with water depth; 65, 90, 80% of mortality at 20, 50, 80 cm water-depth, respectively. Filtering rate (FR) showed the highest value at 50 cm water depth, and thereby the concentration of chlorophyll-${\alpha}$ decreased continuously by 94% of the control at the end of the experiment. In contrast, FR decreased by 34% of the initial concentration at 20 cm water depth. Over the given water-depth range, the mussel FR ranged from $0.15{\sim}0.20L\;gAFDW^{-1}hr^{-1}$ during the 18hrs of experiment, and thereafter, they appeared to be approximately 0.11, 0.26 and 0.30 L $gAFDW^{-1}hr^{-1}$ at 20, 50 and 80cm water depth, respectively. FR was highest with the value of 0.46L $gAFDW^{-1}hr^{-1}\;at\;0.5mgO_2 L^{-1}$ at the early stage of the experiment, while it increased with DO concentration. Maximum pseudofaeces production was 11.2 mg $gAFDW^{-1}hr^{-1}\;at\;9.0mgO_2L^{-1}$. Our results conclude that U. douglasiae has a potential to enhance water quality in eutrophic lake by removing dominant cyanobacteria, but their effects vary with environmental parameters and the water depth at which they are located.

• Korean Journal of Ecology and Environment
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• v.41 no.2
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• pp.237-246
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• 2008

Filtration rates and fecal production of freshwater bivalve, Unio douglasiae on two kinds of hibernal diatom communities were measured simultaneously in a laboratory. One community is the Han River (HAN), which dominated by Asterionella Formosa. Stephanodiscus hantzschii (ca. 98% of total phytoplankton). The other community is the Ilgam Lake (IL), which dominated by Synedra ulna, Scenedesmus sp. Microcystis aeruginosa (ca. 82%). The HAN water has higher concentrations of nutrient (TN and TP) and chlorophyll $\alpha$ (Chl-$\alpha$), lower turbidity and conductivity than the IL water. Water sampling for the feeding experiment was conducted in the same day (Jan 15, 2008) and similar time (AM 10:00 for HAN, AM 11:00 for IL). Mussels with the similar size ($0.0{\pm}0.5\;cm$) were collected from the Gunsan and Okgu district (Jeonbuk), and starved in a laboratory for 2 days before the experiment. The experiment comprised CON (no addition of mussel), LOW (addition of mussel at 0.3 indiv. $L^{-1}$), MID (1.0 indiv. $L^{-1}$) and HIGH (2.0 indiv. $L^{-1}$), respectively. With the increment of mussel density and time, the concentration of Chl-$\alpha$ in two diatom communities were clearly decreased; Chl-$\alpha$ of HAN gradually decreased after 1 hour of mussel treatment, while that of IL decreased as soon as mussel introduction. In 7 hours of treatment, the former was removed finally up to about 90% of control, while the later was remained as about 50%. Under the presence of mussel, total phytoplankton density was shifted as the similar patterns to that of Chl-$\alpha$ (r=0.705, P<0.0001), however, there showed the drastic differences following a species. Based on the concentration of Chl-$\alpha$, filtration rate of U. douglasiae averaged 0.266 $L\;g^{-1}\;h^{-1}$ (0.115 to 0.442) on HAN and $0.577\;L\;g^{-1}\;h^{-1}$ (0.146 to 1.428) on IL water, respectively. There were no differences in feces production among the mussel density in the HAH water (ANOVA, P>0.5), while in IL water, including lots of seston, the HIGH mussel produced the higher fecal materials, over one hundred times of LOW. These results suggest that freshwater bivalve Unio douglasiae have the alternative potential, as a filter-feeder of seston in turbid lake, and a biological controller of diatom bloom in cold stream.

• Restorative Dentistry and Endodontics
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• v.36 no.5
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• pp.425-430
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• 2011

Objectives: This in vitro study investigated whether short-term application of calcium hydroxide in the root canal system for 1 and 4 wk affects the fracture strength of human permanent teeth. Materials and Methods: Thirty two mature human single rooted mandibular premolars in similar size and dentin thickness without decay or restorations were hand and rotary instrumented and 16 teeth vertically packed with calcium hydroxide paste and sealed coronally with caviton to imitate the endodontic procedure and the other 16 teeth was left empty as a control group. The apicies of all the samples were sealed with resin, submerged in normal saline and put in a storage box at $37^{\circ}C$ to mimic the oral environment. After 1 and 4 wk, 8 samples out of 16 samples from each group were removed from the storage box and fracture strength test was performed. The maximum load required to fracture the samples was recorded and data were analysed statistically by the two way ANOVA test at 5% significance level. Results: The mean fracture strengths of two groups after 1 wk and 4 wk were similar. The intracanal placement of calcium hydroxide weakened the fracture strength of teeth by 8.2% after 4 wk: an average of 39.23 MPa for no treatment group and 36.01 MPa for CH group. However there was no statistically significant difference between experimental groups and between time intervals. Conclusions: These results suggest that short term calcium hydroxide application is available during endodontic treatment.

• Economic and Environmental Geology
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• v.35 no.2
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• pp.103-112
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• 2002

Despite the ecological importance of potentially phytotoxic $Al_{13}$-tridecamer and its formation in the simulated condition, it was not recognized in the natural soil environment. Here we performed thermodynamic calculations to examine the stability condition of $Al_{13}$-tridecamer based on the solubility of AI in the Bo horizon of Andisols, Jeju Island, dominantly composed of AI-containing solid phases such as $Al(OH)_{3}$, proto-imogolite and/or imogolite. We have found that $Al(OH)_{3}$, proto-imogolite and/or imogolite may control Al solubility in the moderate acid condition. It means that AI total activity of the soil solution equilibrated with these solid phases ranges from $10^{-6}$ ~ $10^{-8}$M in the pH 5 to 7. Calculations based on the thermodynamic data strongly indicate that the formation of $Al_{13}$-tridecamer closely related to the total activity of AI in the system. For example, for the formation of $Al_{13}$-tridecamer of $10^{-5}$M, Al total activity of $3{\times}10^{-3}$M are needed at pH 4, and $2{\times}10^{-5}$M in the pH 5 to 7. Therefore, this research and the thermodynamic consideration suggest strongly that $Al_{13}$-tridecamer should be negligible in natural soils, especially Andisols and Spodosols, These mainly contain $Al(OH)_{3}$, proto-imogolite and/or imogolite, which could prevent the formation of $Al_{13}$-tridecamer by controlling the AI total activity low. It means that the toxicity of $Al_{13}$-tridecamer with the increase of soil acidification may be considered to be definitely low.

• Korean Journal of Environmental Agriculture
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• v.18 no.3
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• pp.196-203
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• 1999

To establish the best rice cultivating system in the aspects of environment-loving agriculture, the amounts and patterns of nitrogen leached in the paddy soil were investigated with 7 treatments; Recommendation(R), Farmer's usual practice(FUP), Straw compost+chemical fertilizers reduced(SCF), Fresh straw+recommendation(FSC), Fresh cow manure(FCM), Cow manure compost(CMC), and no fertilization as Control(C). And SCF, FCM and CMC were applied with same amounts of total nitrogen to R. The infiltrated water samples were collected in ceramic porous cups which were buried at 60cm depth from the top. Concentrations of nitrate-N in irrigated water were $1.3mg\;l^{-1}$ on rice transplanting season when nutrients began to elute from paddy soil, and $0.4mg\;l^{-1}$ after breaking off irrigation. But it was $4-6mg\;l^{-1}$ in rice growing period. The maximum concentration of nitrate-N in leachate was not more than $7mg\;l^{-1}$ during rice cultivation. The amounts of nitrogen leached in R, FUP, SCF, FSR, FCM, CMC and C were 59, 63, 25, 41, 24, 27, $17kg\;ha^{-1}y^{-1}$ respectively. Nitrogen leaching was decreased to about 30% by supplement of fresh rice straw(FSC) to R. Furthermore, it was possible to reduce to over 50% of nitrogen leaching by reducing chemical fertilizer application(CF), or by substituting of chemical fertilizers with fresh cow manure(FCM) or cow manure compost(CMC). In added organic fertilizer treatments, the amounts of infiltrated nitrogen were less $13-46kg\;ha^{-1}y^{-1}$ than that of input by irrigation. This experiment showed that nutrients leaching was minimized by substitution of chemical fertilizers with organic fertilizer or by application of straw with chemical fertilizers in rice paddy soil and rice cultivation with suitable fertilizer management can work as a purifier rather than contaminator of water.

• Journal of Life Science
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• v.21 no.3
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• pp.385-392
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• 2011

The otter (Lutra lutra) in Korea is classified as a first grade endangered species and is managed under state control. We performed a phylogenetic analysis of the otter that inhabits the Changnyeong, Jinju, and Geoje areas in Gyeongsangnamdo, Korea using mtDNA and microsatellite (MS) markers. As a result of the analysis using the 676-bp D-loop sequence of mtDNA, six haplotypes were estimated from five single nucleotide polymorphisms. The genetic distance between the Jinju and Geoje areas was greater than distances within the areas, and the distance between Jinju and Geoje was especially clear. From the phylogenetic tree estimated using the Bayesian Markov chain Monte Carlo analysis by the MrBays program, two subgroups, one containing samples from Jinju and the other containing samples from the Changnyeong and Geoje areas were clearly identified. The result of a parsimonious median-joining network analysis also showed two clear subgroups, supporting the result of the phylogenetic analysis. On the other hand, in the consensus tree estimated using the genetic distances estimated from the genotypes of 13 MS markers, there were clear two subgroups, one containing samples from the Jinju, Geoje and Changnyeong areas and the other containing samples from only the Jinju area. The samples were not identically classified into each subgroup defined by mtDNA and MS markers. It could be inferred that the differential classification of samples by the two different marker systems was because of the different characteristics of the marker systems used, that is, the mtDNA was for detecting maternal lineage and the MS markers were for estimating autosomal genetic distances. Nonetheless, the results from the two marker systems showed that there has been a progressive genetic fixation according to the habitats of the otters. Further analyses using not only newly developed MS markers that will possess more analytical power but also the whole mtDNA are needed. Expansion of the phylogenetic analysis using otter samples collected from the major habitats in Korea should be helpful in scientifically and efficiently maintaining and preserving them.