• 지구물리와물리탐사
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• 제18권4호
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• pp.181-196
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• 2015

3.5 kHz 또는 첩(chirp) 천부해저 지층탐사는 해양지질 조사나 엔지니어링 탐사에 널리 사용되고 있다. 그러나 해상에서의 너울은 탐사자료의 품질을 저하시킨다. 이와 같은 너울의 영향을 보정함으로써 연속성이 향상된 탐사자료를 얻을 수 있다. 정확한 해저면의 위치 선정은 너울영향 보정에 매우 중요하다. 이 연구에서는 원자료와 이를 엔벨로프 또는 에너지비율자료로 변형시킨 자료들에 대해 최대 진폭값의 일정 기준을 초과하는 지점을 선정하는 방법으로 해저면 위치 선정을 시도하였다. 그러나 파도의 잡음으로 인하여 해저면 신호가 분명하지 않은 품질이 낮은 자료에서는 개별 트레이스에서의 자동적인 해저면 위치 선정이 어려웠다. 이 연구에서는 이전 트레이스에서 구한 해저면 평균값을 고려하여 해저면 선정범위 내에서 해저면을 선정하는 방법과, 선정 결과의 신뢰도가 낮은 경우에는 이를 보정에서 제외하는 방법을 사용함으로써 품질이 낮은 자료의 해저면 선정에서도 만족스러운 결과를 얻었다. 개별 트레이스에서 해저면을 선정할 때에는 에너지비율자료를 사용한 경우에 오류가 가장 적었으며, 이전 트레이스 해저면 평균값을 고려하는 방법에서는 원자료를 직접 사용한 경우에 보정결과가 비교적 양호하였다.

• BMB Reports
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• 제40권5호
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• pp.611-616
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• 2007

$E^{rns}$ is an envelope glycoprotein of classical swine fever virus (CSFV) and has an unusual feature of RNase activity. In the present study, we demonstrate that $E^{rns}$ counteracts Newcastle disease virus (NDV)-mediated induction of IFN-$\beta$. For this purpose, $E^{rns}$ fused to the enhanced green fluorescent protein (EGFP) was transiently expressed in porcine kidney 15 (PK15) cells. In luciferase activity assay, $E^{rns}$-EGFP was found to prevent IFN-$\beta$ promoter-driven luciferase expression and block the induction of IFN-$\beta$ promoter mediated by NDV in a dose-dependent manner. Through IFN-specific semi-quantitative RT-PCR detection, obvious decrease of IFN-$\beta$ mRNA in NDV-infected PK15 cells was observed in the presence of $E^{rns}$-EGFP. In contrast, EGFP alone showed none of this block capacity. In addition, $E^{rns}$-EGFP mutations with RNase inactivation were also found to block NDV-mediated induction of IFN-$\beta$. These evidences establish a novel function for CSFV $E^{rns}$ glycoprotein in counteraction of the IFN-$\beta$ induction pathway.

• Annals of Clinical Neurophysiology
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• 제6권2호
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• pp.65-74
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• 2004

Limb-girdle muscular dystrophy (LGMD) is a heterogeneous group of inherited muscle disorders caused by the mutations of different genes encoding muscle proteins. In the past, when the molecular diagnostic techniques were not available, the subtypes of muscular dystrophies were classified by the pattern of muscle weakness and the mode of inheritance, and LGMD had been considered as a 'waste basket' of muscular dystrophy because many unrelated heterogeneous cases with 'limb-girdle' weakness were put into the category of LGMD. With the advent of molecular genetics at the end of the last century, it has been known that there are many subtypes of LGMD caused by the mutation of different genes, and now, LGMD is classified according to the results of the linkage analysis and the genes or proteins affected. Only small proportion (probably less than 10%) of LGMD is dominantly inherited, and autosomal dominant LGMD (AD-LGMD) consists of six subtypes (LGMD1A to 1F) so far. In autosomal recessive LGMD (AR-LGMD), more than 10 subtypes (LGMD2A to 2J) have been linked and most of the causative genes have been identified. Among AR-LGMDs, LGMD2A (calpain 3 deficiency), 2B (dysferlin deficiency), and sarcoglycanopathy (LGMD2C-2F) are major subtypes. The defective proteins in LGMDs are components of nuclear envelope, cytosol, sarcomere, or sarcolemma, and seem to play a different role in the pathogenesis of muscular dystrophy. It is notable that many causative genes of LGMDs are also responsible for other categories of muscular dystrophy or diseases affecting other tissue. However, by which mechanism they produce such a broad phenotypic variability is still unknown. The identification of mutation in the relevant gene is confirmative for the diagnosis, and is essential for genetic counseling and antenatal diagnosis of LGMD. Because many different genes are responsible for LGMD, differentiation of subtypes using immunohistochemistry and western blotting is the essential step toward the detection of mutation. For the effective research and medical care of the patients with muscular dystrophy in Korea, a research center with a medical facility supported by the government seems to be needed.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.215-220
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• 2000

Abstract White spot disease (WSD), resulting in more than 90% mortality of aquacultured penaeid shrimp, has been reported off the southern and western coasts of Korea since 1993. The pafuogen of WSD has been identified as being a virion wifu an envelope around a central nucleocapsid, and with an average size of 167 nm in diameter and 375 nm in length. In the present study, an in situ hybridization technique was developed as a rapid. sensitive, and specific diagnostic assay for the WSD viros infection in shrimp. Furthermore. the pathological changes ofWSD, in shrimp experimentally infected with WSD viroses. were investigated. Using a biotinylated 643 bp probe obtained from a peR using primers specific to the rod-shaped virus of Penaeus japonicus (RV-PJ), positive signals were detected in both naturally and experimentally infected shrimps. The in situ hybridization revealed positive reactions in the nuclei of the stromal matrix cells in the lymphoid organ, epithelia of the gills, foregut. epidermis, and hematopoietic cells of the interstitial tissues, suggesting the presence of WSD virus. Tills result indicates that the in situ hybridization method can be useful for a rapid and sensitive detection of WSD viruses in shrimp.shrimp.

• 한국전자통신학회논문지
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• 제12권1호
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• pp.53-60
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• 2017

현재 대부분의 초음파를 이용한 거리 측정 기술은 송신부로부터 수신기까지 초음파가 진행하는 시간 즉, TOF(: Time of Flight)를 측정하여 그 동안 음파가 진행한 거리를 산출하는 방법을 활용한다. 이 경우 거리 측정 정확도를 높이기 위해서는 정확한 TOF의 측정이 필요하다. 본 논문은 정확한 TOF를 산출하기 위해 초음파 송수신기의 수학적 모델을 이용하여 수신된 파형의 수학적 모델을 얻어내고 이를 바탕으로 TOF를 산출하는 방식을 제안한다. 이 방식은 트리거링 후부터 수신된 파형를 이미 얻어진 수학적 모델과 최소자승법을 이용하여 비교하여 초음파 신호가 수신기에 도달한 시점을 역으로 추정하는 방식이다. 제안된 방법을 사용할 경우 트리거링 시점의 변동에 의한 영향을 줄일 수 있음을 실험을 통하여 확인하였다.

• 천문학회지
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• 제47권6호
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• pp.293-302
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• 2014

Simultaneous time monitoring observations of $H_2O$ $6_{16}-5_{23}$, SiO J = 1-0, 2-1, 3-2, and $^{29}SiO$ ${\upsilon}=0$, J = 1-0 lines are carried out in the direction of the Mira variable star TX Cam with the Korean VLBI Network single dish radio telescopes. For the first time, the $H_2O$ maser emission from TX Cam is detected near the stellar velocity at five epochs from April 10, 2013 (${\phi}=3.13$) to June 4, 2014 (${\phi}=3.89$) including minimum optical phases. The intensities of $H_2O$ masers are very weak compared to SiO masers. The variation of peak antenna temperature ratios among SiO ${\upsilon}=1$, J = 1-0, J = 2-1, and J = 3-2 masers is investigated according to their phases. The shift of peak velocities of $H_2O$ and SiO masers with respect to the stellar velocity is also investigated according to observed optical phases. The $H_2O$ maser emission occurs around the stellar velocity during our monitoring interval. On the other hand, the peak velocities of SiO masers show a spread compared to the stellar velocity. The peak velocities of SiO J = 2-1, and J = 3-2 masers show a smaller spread with respect to the stellar velocity than those of SiO J = 1-0 masers. These simultaneous observations of multi-frequencies will provide a good constraint for maser pumping models and a good probe for investigating the stellar atmosphere and envelope according to their different excitation conditions.

• BMB Reports
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• 제31권1호
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• pp.58-63
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• 1998

In this study, a mouse monoclonal antibody (mAb) against gp41(584-618), the immunodominant epitope protein, was generated. For this purpose, BALB/c mice were immunized with double branched multiple antigenic peptides derived from the HIV-1 gp41(584-618) sequence, and antibody-secreting hybridoma were produced by fusion of mice splenocytes with SP2/0 myeloma cells. One clone producing an antigen specific mAb, termed KI-41(isotype IgG1) was identified, whose specific reactivity against gp41(584-618) could be confirmed by ELISA and Western blot analysis. Epitope mapping revealed the recognition site of the mAb KI-41 to be located around the sequence RILAVERYLKDQQLLG, which comprises the N-terminal region within the immunized gp41(584-618) peptied. Since this mAb recognizes this specific epitope within the HIV-1 gp41 without any cross-reactivity to other immunodominant regions in the HIV-2 gp35, KI-41 will provide some alternative possibilities in further applications such as the development of indirect or competitive ELISA for specific antibody detection in HIV-1 infection or for other basic researches regarding the role and function of HIV-1 gp41.

• 한국소음진동공학회논문집
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• 제24권7호
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• pp.555-561
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• 2014

Acoustic Emission technique is widely applied to develop the early fault detection system, and the problem about a signal processing method for AE signal is mainly focused on. In the signal processing method, envelope analysis is a useful method to evaluate the bearing problems and wavelet transform is a powerful method to detect faults occurred on rotating machinery. However, exact method for AE signal is not developed yet for the rotating machinery diagnosis. Therefore, in this paper two methods which are processed by Hilbert transform and DET for feature extraction. In addition, we evaluate the classification performance with varying the parameter from 2 to 15 for feature selection DET, 0.01 to 1.0 for the RBF kernel function of SVR, and the proposed algorithm achieved 94 % classification of averaged accuracy with the parameter of the RBF 0.08, 12 feature selection.

• 한국음향학회지
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• 제32권2호
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• pp.138-146
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• 2013

수동 소나는 데몬 처리를 통해 수중 표적의 방사 소음으로부터 프로펠러 정보를 분석한다. 기존 데몬 처리 기법은 신호대 잡음비(Signal to Noise Ratio)를 높이기 위해 시간 영역에 대해 음향 신호를 분할하여 중첩 처리하는 방법을 사용하였다. 다시 말해 일정 시간동안 음향 신호를 수집 및 분할 처리 후 평균을 취해 잡음의 분산(variance)을 감소시켜 신호대 잡음비를 향상시켰다. 그러나 이러한 방법은 각 처리 구간의 음향 신호가 서로 독립적이지 않아 높은 성능 향상을 위해서는 많은 시간과 연산량이 필요하다. 따라서 본 논문에서는 대역 분할 처리를 통한 데몬 처리 성능 향상 기법을 제안한다. 제안된 기법은 주파수 영역에서 음향 신호의 구간을 분할하여 데몬 처리를 수행하므로 기존 중첩 기법에 비해 짧은 시간안에 서로 독립적 음향 신호 수집이 가능하다. 따라서 기존 기법에 비해 적은 시간과 연산량으로 동일한 성능을 발휘할 수 있다. 제안된 기법은 수학적 분석 및 시뮬레이션을 통해 기존 기법에 비해 성능이 우수함을 검증하였다.

• 천문학회보
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• 제39권1호
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• pp.63.2-63.2
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• 2014

As a part of the "Dust, Ice, and Gas In Time" (DIGIT) key program on Herschel, we observed GSS30-IRS1, a Class I protostar located in Ophiuchus (d =125 pc), with Herschel/Photodetector Array Camera and Spectrometer (PACS). More than 70 lines were detected within a wavelength range from 50 ${\mu}m$ to 200 ${\mu}m$: CO lines from J = 14-13 to 41-40, several $H_2O$ lines of Eup = 100 K to 1500 K, 16 transitions of OH rotational lines, and two atomic [O I] lines at 63 and 145 ${\mu}m$. The [C II] line, known as a tracer of externally heated gas by the interstellar radiation field, is also detected at 158 ${\mu}m$. All lines, except [O I] and [C II], are detected only at the central spaxel of $9^{\prime\prime}.4{\times}9^{\prime\prime}.4$. The [O I] emission is extended along a NE-SW orientation, which is consistent with the known outflow direction, while the [C II] line is detected over all spaxels. One possible explanation of the detection of the [C II] line and no correlation of its spatial distribution with any other molecular emission is the existence of the enhanced ISRF nearby GSS30-IRS1. One interesting feature of GSS30-IRS1 is that the continuum emission is extended beyond the point-spread function (PSF), unlike the molecular line emission, indicative of significant external heating. The best-fit continuum model of GSS30-IRS1 with the physical structure including flared disk, envelope, and outflow shows that the internal luminosity is 11 $L_{\odot}$, and the region is also externally heated by a radiation field enhanced by a factor of 25 compared to the local standard interstellar field.