• Journal of Environmental Science International
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• v.3 no.3
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• pp.229-239
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• 1994

By laying emphasis on the intermediate layer, water property distribution in the Northwest Pacific is studied using the hydrographic data obtained by Japan Meteorologica] Agency in the period from 1960 to 1986. The scattering of water type in T-S diagram is relatively small in the Kuroshio Region. Both the envelopes of saline side and of fresh side of the scattered data points shifts gradually from saline side to fresh side as the observation line moves from southwest to northeast. In the Mixed Water Region, the scattering of water type increases rapidly as the observation line moves north; The envelope of fresh cold side moves towards fresh cold side much faster than that of same side. The thermosteric anomaly value at the salinity minimum decreases as the observation line moves from north to south or southwest. This suggests that the water does not advect along the salinity minimum layer, but that the salinity minimun layer is understood as a boundary of two different waters aligned vertically. We defined the typical water masses for the Oyashio Water and the Kuroshio Water. The water mass below the salinity minimum layer may be created by isopycnal mixing of these two water masses with a fixed mixing rate. While, the water mass above the salinity minimum cannot be created simply by isopycnal mixing. The salinity minimum layer may be eroded from upper side due to active mixing processes in the surface layer, while the water of the salinity minimum layer moves gradually southward. This appears to give an explanation why the thermosteric anomaly value at salinity minimum decreases towards south.

• Culinary science and hospitality research
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• v.19 no.1
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• pp.204-214
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• 2013

The purpose of this study is to identify instant noodle intake and consumption patterns of adults in metropolitan areas of Korea. There was a total of 702 subjects, 18 years of age and above, and data from the self-administrated questionnaires were collected and analyzed using comparative statistical analysis, including frequency, chi-square, t-test, and one-way ANOVA. According to the results, preference level for instant noodles among the adults was indicated to be $4.84{\pm}1.32$ and 54% of subjects either "liked" or "more than liked" noodles. People in 20s liked them the most. Noodles in envelope type package with soup are highly preferred. The most common reason why they chose to eat instant noodles was "convenience." At the same time, people avoid them because of their bad nutrition and calories. Half of the subjects consume instant noodles at least once a week mainly for lunch and dinner as a substitute for their meal. In the case of eating-together-with-family was the largest. 72.4% of subjects consumed entire soup additive content and 20.6% of them eat almost without soup because of being full and worrying that it might be harmful to health. Significant difference was shown in the preference level and intake patterns depending on respondents' general characteristics. Limitations and future research directions are also discussed.

• The Journal of Engineering Geology
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• v.19 no.2
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• pp.153-163
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• 2009

In this study, a series of triaxial tests on Jeju basalt were carried out and then rock strength parameters were estimated by the Mohr-Coulomb failure criterion and the Hoek-Brown failure criterion using the test results. The characteristics of both failure criterions were investigated through comparing the estimated rock strength parameters. As the result of the Mohr-Coulomb criterion, the cohesions and the internal friction angles are determined as 5.35 MPa and $50.25^{\circ}$ of Pyoseonri basalt, 16.99 MPa and $60.66^{\circ}$ of Trachy-basalt, and 2.33 MPa and $37.05^{\circ}$ of Scoria, respectively. The cohesions and internal friction angles were estimated by the Hoek-Brown failure criterion in the basis of the results of regression analysis. The cohesions and the internal friction angles are determined as 4.77 MPa and $52.47^{\circ}$ of Pyoseonri basalt, 14.69 MPa and $60.70^{\circ}$ of Trachy-basalt, and 2.22 MPa and $47.60^{\circ}$ of Scoria, respectively. As the result of comparison between the Mohr-Coulomb failure criterion and the failure envelope predicted by the Hoek-Brown criterion, the cohesion estimated by the Hoek-Brown criterion is usually lower than that obtained from the Mohr-Coulomb criterion, whereas the friction angle estimated by the Hoek-Brown criterion is higher than that obtained from the Mohr-Coulomb criterion.

• Development and Reproduction
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• v.11 no.2
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• pp.97-104
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• 2007

This study describes the spermatogenesis and sperm ultrastructure of the spiny top shell, Batillus cornutus using light and electron microscopy. The spiny top shells were collected by divers in the coastal water of Wandogun, Cheollanamdo, Korea(N $34^{\circ}13'$, E $126^{\circ}47'$) at May 2003. Spiny top shells of $60.0{\sim}69.9\;mm$ in shell height were used in this study. The testis comprises many spermatogenic follicles which contains germ cells in different developmental stages. The primary spermatocytes in the pachytene stage were characterized by synaptonemal complexes. The early spermatids were characterized by appearance of Golgi complex, increased karyoplasmic electron density and tubular mitochondria. In early spermatid the mass of proacrosomal granules consists of numerous heterogeneous granules with high electron density. From the mid-stage of spermiogenesis the well-developed mitochondria aggregate posterior to the nucleus, and surround the proximal and distal centrioles. In this stage, proacrosomal granules are condensed and form a acrosome with thin envelope. During the late spermiogenesis, the acrosome begins to elongate and then became conical. The sperm consists of head, mid-piece and tail. The head comprises a round nucleus and a conical acrosome. Acrosomal rod of microfibrous is observed between nucleus and acrosome. Five mitochondria observed in mid-piece. And tail has the typical "9+2" microtubular system originates from the centrioles.

• Korean Journal of Food Science and Technology
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• v.52 no.5
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• pp.467-475
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• 2020

Ultraviolet radiation is a major cause of photoaging, characterized by wrinkle formation and skin dehydration. Mojaban (Sargassum fulvellum), belonging to the brown algae of the Sargassaceae family, exerts anti-inflammatory, antioxidant, and anti-atopic activities. Particularly, Mojaban extract alleviated ultraviolet B (UVB)-induced inflammatory responses in human keratinocytes and BALB/c mice. Here, the dorsal skin of hairless mice was exposed to UVB for photoaging. The mice were administrated 300 mg/kg Mojaban extract (S. fulvellum extract; SFE) daily. Phenotypically, SFE reduced wrinkle formation and epidermal thickness and increased skin elasticity. SFE increased hydroxyproline content by upregulating the expression of collagen-related genes and downregulating matrix metalloproteinase expression. Furthermore, SFE increased skin moisture content and inhibited transepidermal water loss. SFE upregulated the expression of involucrin, loricrin, and transglutaminase for cornified envelope formation as well as the expression of filaggrin and caspase-14 for natural moisturizing factors. Collectively, SFE may be employed as a nutraceutical ingredient for anti-photoaging.

• Korean Journal of Organic Agriculture
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• v.24 no.4
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• pp.945-955
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• 2016

To date, chemical managements of plant bacterial diseases are complicated by limitations on use of antibiotics in agriculture, antibiotic resistance development, and limited efficacy of alternative control agents. In this study, thus, we performed screening of eco-friendly farming material (notice no. 2-4-064) containing tannic acid as new antibacterial-activity against 7 plant bacterial pathogens: Pectobacterium carotovorum subsp. carotovorum (Pcc), Ralstonia solanacearum (Rs), Acidovorax avenae subsp. citrulli (Aac), Xanthomonas cirti pv. citri (Xcc), Erwinia pyrifoliae (Ep), Clavibacter michiganensis subsp. michiganensis (Cmm), and Streptomyces scabies (Sc), Initial screening of antibacterial effects of eco-friendly farming material was performed using the paper disk diffusion method and co-cultivation in broth culture. Tannic acid based eco-friendly farming material showed inhibitory effect against Pcc, Rs, Aac, Xcc, Cmm, and Ss, whereas it did not show inhibition zone against Ep. However, when it tested by co-cultivation in broth culture, it showed strong inhibition effect against all pathogens that declined growth curve compared to bacterial pathogen only. Interestingly, when we observed morphological changes on those pathogens by SEM, cell morphologies of 7 pathogens were slightly changed that seem to be malfunction in their cell envelope.

• Tunnel and Underground Space
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• v.10 no.2
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• pp.180-195
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• 2000

Grouting has been practiced as a reliable technique to improve the mechanical properties of rock mass. But, the study of ground improvement by greeting is rare especially in jointed rock mass. In this study, joint compression test and direct shear test were performed on pure rock joint and cement milk grouted rock joint to examine the grouting effect on the property of rock joint. In the pure rock joint compression test, joint closure varied non-linearly with normal stress. But after cement milk grouting, the normal deformation characteristics of the joint was linear at the low normal stress level. As normal stress increased. deformation of the sample rapidly increased due to the stress concentration at the joint asperities. Peak shear strength of the grouted joint in low normal stress was higher than that of non-grouted joint due to the cohesion, decreased exponetially as the grout thickness increased. Thus after cement milk grouting, the failure envelope modified to a curve that has cohesion due to grout material hydration with decreased friction angle. Shear stiffness and peak dilation angle of the grouted joint decreased as the grout thickness increased. The peak shear strength from the direct shear test on grouted rock joint was represented by an empirical equation as a fuction of grout thickness and roughness mean amplitude.

• Korean Journal of Veterinary Research
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• v.37 no.4
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• pp.883-897
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• 1997

Present study was undertaken in order to find out the most significant laboratory test for the early diagnosis of ethylene glycol poisoning in dogs. Seven dogs weighing from 5.0kg to 19.3kg were administered orally 6ml or l0ml of ethylene glycol per kilogram of body weight. In addition to clinical observations, blood samples were taken from the jugular vein on 0, 1, 3, 6, 12, 24 and 48 hours or until death after the adminisration and examined for the hematological, blood chemical, venous blood gas and electrolyte changes. Urine samples were simultaneously taken and examined for pH, protein, glucose and calcium oxalate crystals in the sediments. The results were as follows : 1. All dogs showed marked hypothermia in addition to the characteristic signs of ethylene glycol poisoning until death. 2. No significant hematological changes were observable after the administration of ethylene glycol except the secondary dehydration and stress leukogram. 3. Chemical values related to the liver function(serum glutamic pyruvic transaminase, total serum protein, serum albumin, serum total bilirubin) showed no significant changes during the experimental period in all dogs. 4. Chemical values related to the renal function(BUN and creatinine) showed significant(p<0.0l, p<0.001) increase from 24 hours to death in all dogs. 5. All dogs showed significant(p<0.01, p<0.001) decrease in blood pH from 3 hours to death after the ethylene glycol administration. 6. All dogs showed significant(p<0.05, p<0.01, p<0.001) increaes in anion gap from 1 hour to death after the ethylene glycol administration. 7. All dogs showed significant(p<0.05, p<0.01) decrease in urine pH from 1 hour to 6 hours after the administration of ethylene glycol. 8. Characteristic envelope form of calcium oxalate crystals were found in the urinary sediment from 6 hours after the administration of ethylene glycol in all dogs. 9. Histologically calcium oxalate crystals were found in the renal rubles in all dogs. 10. From these results, it may be concluded that the examinations of urinary sediments for the calcium oxalate crystals, blood or urinary pH and blood anion gap are most reliable tests for the early diagnosis of ethylene glycol poisoning in dogs.

• Journal of Microbiology
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• v.42 no.1
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• pp.25-31
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• 2004

Sf9 cells have obvious advantages for the conventional production technology of vaccine. They are useful tools for high concentration and large-scale cultures. Sf9 cells were grown to maximal concentration, 8${\times}$l0$\^$6/ cells/$m\ell$ in a 500$m\ell$ spinner flask, with a doubling time at the exponentially growing phase of 24.5 hours, using serum-free media. To explore the ability of Sf9 cells to be infected by the Japanese encephalitis (JE) virus Beijing-l strain, Sf9 cells were infected with the virus. By 4-5 days post-infection, 10-15 % of the Sf9 cells showed cytopathic effect (CPE), from granularity to the formation of syncytia and multinucleated giant cells continuously observed over a period of 35 days. Positive fluorescent reactions were detected in 30-40% of cells infected with the JE virus Beijing-l strain, and the uninfected Sf9 cells were completely negative. Virus particles, propagated in Sf9 and Vero cells, were concentrated by sedimentation on 40% trehalose cushions by ultracentrifugation, and showed identical patterns of viral morphogenesis. Complete virus particles, 40 to 50 nm in diameter, were observed, and JE virus envelope (E) proteins, at 53 kDa, were found in the western blot analysis to the anti-JE virus E protein monoclonal antibody and reacted as a magenta band in the same position to the glycoprotein staining. To evaluate whether the infectious virus was produced in Sf9 cells inoculated with the JE virus Beijing-l stain, Sf9 cells were inoculated with the virus, and sample harvested every 5 days. The titers of the JE virus Beijing-l strain rose from 1.0${\times}$l0$\^$5/ to 1.5${\times}$l0$\^$6/ pfu/$m\ell$. The infected Sf9 cells could be subcultured in serum-free medium, with no change in the plaque sizes formed by the JE virus Beijing-l strain in the plaque assay. It is suggested that the ability of the JE virus Beijing-l strain to infect Sf9 cells in serum-free media will provide a useful insect cell system, where the JE virus replication, cytopathogenicity and vaccine immunogen can be studied.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.19 no.6
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• pp.1-8
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• 2005

The quantity of light available for a space can be translated in term of the amount of energy savings through a process of a building energy simulation. To get significant energy savings in general illumination, the electric lighting system must be incorporated with a daylight - activated dimmer control. A prototype configuration of an once interior has been established and the integration between the building envelope and lighting and HVAC systems is evaluated based on computer modeling of a lighting control facility. First of all, an energy-efficient luminaire system is designed and the lighting analysis program, Lumen-Micro 2000 predicts the optimal layout of a conventional fluorescent lighting future to meet the designed lighting level and calculates unit power density, which translates the demanded met of electric lighting energy. A dimming control system integrated with the contribution of daylighting has been applied to the operating of the artificial lighting. Annual cooling load due to lighting and the projecting saving amount of cooling load due to daylighting under overcast diffuse sky m evaluated by computer software ENER-Win. In brief, the results from building energy simulation with measured daylight illumination levels and the performance of lighting control system indicate that daylighting can save over 70 percent of the required energy for general illumination in the perimeter zones through the year A 25[%] of electric energy for cooling and almost all off heating energy may be saved by dimming and turning off the luminaires in the perimeter zones.