• Proceedings of the Korean Society of Life Science Conference
• /
• 2005.09a
• /
• pp.55-55
• /
• 2005

• Proceedings of the Korean Society of Life Science Conference
• /
• 2005.09a
• /
• pp.57-57
• /
• 2005

• Proceedings of the Korean Society of Life Science Conference
• /
• 2005.09a
• /
• pp.56-56
• /
• 2005

• Proceedings of the KTCVS Conference
• /
• 1993.06a
• /
• pp.122-122
• /
• 1993

• 한국생물공학회:학술대회논문집
• /
• 2000.11a
• /
• pp.47-47
• /
• 2000

• Journal of Microbiology and Biotechnology
• /
• v.31 no.2
• /
• pp.250-258
• /
• 2021

Among various species of marine bacteria, those belonging to the genus Halomonas have several promising applications and have been studied well. However, not much information has been available on their antibiotic resistance. In our efforts to learn about the antibiotic resistance of strain Halomonas socia CKY01, which showed production of various hydrolases and growth promotion by osmolytes in previous study, we found that it exhibited resistance to multiple antibiotics including kanamycin, ampicillin, oxacillin, carbenicillin, gentamicin, apramycin, tetracycline, and spectinomycin. However, the H. socia CKY01 resistance pattern to kanamycin, gentamicin, apramycin, tetracycline, and spectinomycin differed in the presence of 10% NaCl and 1% NaCl in the culture medium. To determine the mechanism underlying this NaCl concentration-dependent antibiotic resistance, we compared four aminoglycoside resistance genes under different salt conditions while also performing time-dependent reverse transcription PCR. We found that the aph2 gene encoding aminoglycoside phosphotransferase showed increased expression under the 10% rather than 1% NaCl conditions. When these genes were overexpressed in an Escherichia coli strain, pETDuet-1::aph2 showed a smaller inhibition zone in the presence of kanamycin, gentamicin, and apramycin than the respective control, suggesting aph2 was involved in aminoglycoside resistance. Our results demonstrated a more direct link between NaCl and aminoglycoside resistance exhibited by the H. socia CKY01 strain.

• Proceedings of the Optical Society of Korea Conference
• /
• 2004.07a
• /
• pp.4-5
• /
• 2004

• 한국정보디스플레이학회:학술대회논문집
• /
• 2003.07a
• /
• pp.639-641
• /
• 2003

The parasitic capacitance between the high voltage electrodes and the backlight housing causes lowering lamp current, electric power leakage, and leading to lower brightness and efficiency in a multi-lamps backlight. In this study a new center balance swing operation method is introduced to be minimizing those housing effects.

• Journal of Microbiology and Biotechnology
• /
• v.32 no.1
• /
• pp.27-36
• /
• 2022

Ever since bioplastics were globally introduced to a wide range of industries, the disposal of used products made with bioplastics has become an issue inseparable from their application. Unlike petroleum-based plastics, bioplastics can be completely decomposed into water and carbon dioxide by microorganisms in a relatively short time, which is an advantage. However, there is little information on the specific degraders and accelerating factors for biodegradation. To elucidate a new strain for biodegrading poly-3-hydroxybutyrate (PHB), we screened out one PHB-degrading bacterium, Microbulbifer sp. SOL03, which is the first reported strain from the Microbulbifer genus to show PHB degradation activity, although Microbulbifer species are known to be complex carbohydrate degraders found in high-salt environments. In this study, we evaluated its biodegradability using solid- and liquid-based methods in addition to examining the changes in physical properties throughout the biodegradation process. Furthermore, we established the optimal conditions for biodegradation with respect to temperature, salt concentration, and additional carbon and nitrogen sources; accordingly, a temperature of 37℃ with the addition of 3% NaCl without additional carbon sources, was determined to be optimal. In summary, we found that Microbulbifer sp. SOL03 showed a PHB degradation yield of almost 97% after 10 days. To the best of our knowledge, this is the first study to investigate the potent bioplastic degradation activity of Microbulbifer sp., and we believe that it can contribute to the development of bioplastics from application to disposal.

• Proceedings of the Korean Magnestics Society Conference
• /
• 2003.12a
• /
• pp.76-76
• /
• 2003