• Title/Summary/Keyword: Engineering College

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RNA viruses in Pleurotus ostreatus

  • Lee, Jung-Sun;Lee, Nam-Joo;Ha, Si-Jung;Kim, So-Yeon;Kang, Eun-Joo;Kang, Dong-Suk;Rho, Hyun-Su;Chang, Moo-Ung;Lee, Jong-Kyu;Lee, Kyung-Hee;Lee, Hyun-Sook
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2004.10a
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    • pp.46-46
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    • 2004
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Automatic payment system for public transportation using beacon (비콘 기술을 이용한 교통 요금 자동결제 시스템)

  • Park, Kyu-chan;Park, Ji-Hoon;LEE, Jung-hyun;Na, Su-In;Kyuong, Mee-Lim;Jo, Jung-Seok;Chae, Hwi-Byeong;Oh, Seung-Hyun;Seo, Dan-Bi
    • Proceedings of the KIEE Conference
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    • 2015.07a
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    • pp.113-114
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    • 2015
  • 본 논문에서는 Beacon을 이용한 교통 요금 자동결제 시스템을 제안한다. 제안된 시스템은 특별한 행위 없이 사용자의 대중교통 이용 여부를 정확히 판단하여 자동으로 결제를 처리하기 때문에 편리하게 서비스를 이용할 수 있다. 특히, 승하차 시스템을 설계하기 위하여 필요한 알고리즘과 데이터 처리 과정을 제안하고 그 파급효과를 분석한다.

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Increased Tolerance to Furfural by Introduction of Polyhydroxybutyrate Synthetic Genes to Escherichia coli

  • Jung, Hye-Rim;Lee, Ju-Hee;Moon, Yu-Mi;Choi, Tae-Rim;Yang, Soo-Yeon;Song, Hun-Suk;Park, Jun Young;Park, Ye Lim;Bhatia, Shashi Kant;Gurav, Ranjit;Ko, Byoung Joon;Yang, Yung-Hun
    • Journal of Microbiology and Biotechnology
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    • v.29 no.5
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    • pp.776-784
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    • 2019
  • Polyhydroxybutyrate (PHB), the most well-known polyhydroxyalkanoate, is a bio-based, biodegradable polymer that has the potential to replace petroleum-based plastics. Lignocellulose hydrolysate, a non-edible resource, is a promising substrate for the sustainable, fermentative production of PHB. However, its application is limited by the generation of inhibitors during the pretreatment processes. In this study, we investigated the feasibility of PHB production in E. coli in the presence of inhibitors found in lignocellulose hydrolysates. Our results show that the introduction of PHB synthetic genes (bktB, phaB, and phaC from Ralstonia eutropha H16) improved cell growth in the presence of the inhibitors such as furfural, 4-hydroxybenzaldehyde, and vanillin, suggesting that PHB synthetic genes confer resistance to these inhibitors. In addition, increased PHB production was observed in the presence of furfural as opposed to the absence of furfural, suggesting that this compound could be used to stimulate PHB production. Our findings indicate that PHB production using lignocellulose hydrolysates in recombinant E. coli could be an innovative strategy for cost-effective PHB production, and PHB could be a good target product from lignocellulose hydrolysates, especially glucose.

Experimental Approach for the Estimation of Cardiac Output of Left Ventricular Assist Device Using Multi-dimensional Interpolation Technique

  • Om, K.S.;Choi, W.W.;An, J.M.;Park, S.K.;Jo, Y.H.;Choi, J.S.;Lee, J.J.;Kim, H.C.;Min, B.G.
    • Proceedings of the KOSOMBE Conference
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    • v.1996 no.05
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    • pp.232-234
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    • 1996
  • Cadiac output estimation scheme of LVAD using multi-dimensional interpolation technique was introduced in this paper. This paper also show appropriate input -output data for estimation. Experimental results show our approach is a good one for the estimation of nonlinear hemodynamics.

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Increased Antibiotic Resistance of Methicillin-Resistant Staphylococcus aureus USA300 Δpsm Mutants and a Complementation Study of Δpsm Mutants Using Synthetic Phenol-Soluble Modulins

  • Song, Hun-Suk;Bhatia, Shashi Kant;Choi, Tae-Rim;Gurav, Ranjit;Kim, Hyun Joong;Lee, Sun Mi;Park, Sol Lee;Lee, Hye Soo;Joo, Hwang-Soo;Kim, Wooseong;Seo, Seung-Oh;Yang, Yung-Hun
    • Journal of Microbiology and Biotechnology
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    • v.31 no.1
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    • pp.115-122
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    • 2021
  • Phenol-soluble modulins (PSMs) are responsible for regulating biofilm formation, persister cell formation, pmtR expression, host cell lysis, and anti-bacterial effects. To determine the effect of psm deletion on methicillin-resistant Staphylococcus aureus, we investigated psm deletion mutants including Δpsmα, Δpsmβ, and Δpsmαβ. These mutants exhibited increased β-lactam antibiotic resistance to ampicillin and oxacillin that was shown to be caused by increased N-acetylmannosamine kinase (nanK) mRNA expression, which regulates persister cell formation, leading to changes in the pattern of phospholipid fatty acids resulting in increased anteiso-C15:0, and increased membrane hydrophobicity with the deletion of PSMs. When synthetic PSMs were applied to Δpsmα and Δpsmβ mutants, treatment of Δpsmα with PSMα1-4 and Δpsmβ with PSMβ1-2 restored the sensitivity to oxacillin and slightly reduced the biofilm formation. Addition of a single fragment showed that α1, α2, α3, and β2 had an inhibiting effect on biofilms in Δpsmα; however, β1 showed an enhancing effect on biofilms in Δpsmβ. This study demonstrates a possible reason for the increased antibiotic resistance in psm mutants and the effect of PSMs on biofilm formation.