• Title/Summary/Keyword: Engineering Center

Search Result 30,154, Processing Time 0.054 seconds

Microcontact Printing of Biotin for Selective Immobilization of Streptavidin-fused Proteins and SPR Analysis

  • Lee, Sang-Yup;Park, Jong-Pil;Lee, Seok-Jae;Park, Tae-Jung;Lee, Kyung-Bok;Park, Insung S.;Kim, Min-Gon;Chung, Bong-Hyun
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • v.9 no.2
    • /
    • pp.137-142
    • /
    • 2004

  • In this study, a simple procedure is described for patterning biotin on a glass substrate and then selectively immobilizing proteins of interest onto the biotin-patterned surface. Microcontact printing (CP) was used to generate the micropattern of biotin and to demonstrate the selective immobilization of proteins by using enhanced green fluorescent protein (EGFP) as a model protein, of which the C-terminus was fused to a core streptavidin (cSA) gene of Streptomyces avidinii. Confocal fluorescence microscopy was used to visualize the pattern of the immobilized protein (EGFP-cSA), and surface plasmon resonance was used to characterize biological activity of the immobilized EGFP-cSA. The results suggest that this strategy, which consists of a combination of $\mu$CP and cSA-fused proteins. is an effective way for fabricating biologically active substrates that are suitable for a wide variety of applications. one such being the use in protein-protein assays.

  • PDF KSCI

High voltage MOSFET fabricated by using a standard CMOS logic process to drive the top emission OLEDs in silicon-based OELDs

  • Lee, Cheon-An;Kwon, Hyuck-In;Jin, Sung-Hun;Lee, Chang-Ju;Lee, Myung-Won;Kyung, Jae-Woo;Cho, Il-Whan;Lee, Jong-Duk;Park, Byung-Gook
    • 한국정보디스플레이학회:학술대회논문집
    • /
    • 2003.07a
    • /
    • pp.981-983
    • /
    • 2003

  • Using the conventional standard CMOS logic process, the high voltage MOSFET to drive top emission OLEDs was fabricated for the silicon-based organic electroluminescent display. The drift region of the conventional high voltage MOSFET was implemented by the n-well of the logic process. The measurement result shows a good saturation characteristic up to 50 V without breakdown phenomena.

  • PDF

Swing Phase Dynamic Simulation of Pneumatic Prosthesis (공압식 대퇴의지의 유각기 동역학 시뮬레이션)

  • Cho, H.S.;Ryu, J.C.;Mun, M.S.;Kim, G.S.;Kim, K.H.;Kim, S.K.;Cheon, M.S.
    • Proceedings of the KOSOMBE Conference
    • /
    • v.1997 no.11
    • /
    • pp.267-271
    • /
    • 1997

  • In this study, swing phase dynamic simulation of above-knee prosthesis is performed. The prosthesis consists of a single axis knee mechanism and pneumatic cylinder. The numerical modelling of the prosthesis is analyzed in two dimensions. The governing equation of thermodynamical pneumatic cylinder model is applied to construct the control of lower limb during swing phase. Knee flexion angle with respect to the orifice diameter of the pneumatic cylinder is produced. This analysis will be very useful to the design of pneumatic cylinder in prosthesis.

  • PDF