• BMB Reports
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• v.41 no.7
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• pp.529-536
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• 2008

Cinnamate-4-hydroxylase (C4H) is a key enzyme in the phenylpropanoid pathway, which is responsible for synthesizing a variety of secondary metabolites that participate in development and adaptation. In this study, we isolated a full-length cDNA of the C4H gene from the Korean black raspberry (Rubus sp.) and found that this gene existed as a single gene. By comparing the deduced amino acid sequence of Rubus sp. C4H with other sequences reported previously we determined that this sequence was highly conserved among widely divergent plant species. In addition, quantitative real time PCR studies indicated that the C4H gene had a differential expression pattern during fruit development, where gene expression was first detected in green fruit and was then remarkably reduced in yellow fruit, followed by an increase in red and black fruit. To investigate the two peaks in expression observed during fruit development and ripening, we measured the flavonoid content. The content of the major flavanol of Korean black raspberry fruits was determined to be highest at the beginning of fruit development, followed by a gradually decrease according to the developmental stages. In contrast, the content of anthocyanins during the progress of ripening was dramatically increased. Our results suggest that the C4H gene in Korean black raspberry plays a role during color development at the late stages of fruit ripening, whereas the expression of C4H gene during the early stages may be related to the accumulation of flavanols.

• Journal of Hydrogen and New Energy
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• v.25 no.4
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• pp.405-418
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• 2014

Fuel cells are suitable for a power plant of a unmanned aerial vehicle (UAV) as it is not only environmentally friendly and quiet but also more efficient than an internal combustion engine. A fuel cell hybrid UAV has better performance in endurance than a fuel cell only or battery only UAV. One of the key purposes of making fuel cell hybrid UAVs is having long endurance and now maximum 26 hours of flight is possible. Because optimal design and control methods for fuel cell hybrid UAVs are absolutely needed for their long endurance we have to check the methods. The aircraft made by using application-integrated design method has less BOP mass and better performances. The optimal design and control methods are generally based on computer simulations or Hardware-In-The-Loop simulations by using dynamic models for their design and control. The Hardware-In-The-Loop simulation (HILS) is to use a hardware device like a fuel cell stack as well as a simulation program and it allows for making optimally designed applications. This paper introduce efficient methods of design, control and evaluation for the fuel cell hybrid UAVs.

• Journal of Bio-Environment Control
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• v.21 no.4
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• pp.372-378
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• 2012

This study was carried out in order to determine the optimum length of a roll type PE pipe heat exchanger employed in the water-to-water heat pump system using the waste heat of the heated effluent flowed out from thermal power generation plants as a heat source. And the heat pump system of 30 RT for an experimental test was designed and manufactured. And also PE pipes were employed to recover the waste heat from the heated effluent. The inside diameter of PE pipe heat exchanger was 20 mm, the thickness was 2 mm and the diameter of a roll was 1,000 mm. And from the results of this study, we found that the optimum length of PE pipe heat exchanger was 75 m per the heat pump capacity of 1.0 RT (3.51 kW) and then the heating COP of heat pump system was 3.8.

• Journal of Radiation Industry
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• v.5 no.4
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• pp.347-352
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• 2011

In order to identify the genetic relationship analysis by acute and chronic gamma irradiation, Arabidopsis (Arabidopsis thaliana L.) were irradiated with 200 Gy of gamma-rays using gamma-irradiator (3,000 Ci; Nordion, Canada) and gamma-phytotron (400 Ci; Nordion, Canada) for acute and chronic irradiation, respectively. Genetic relationship among two acute gamma-irradiated plants (A1 and A24) and three chronic gamma-irradiated plants (C1W, C2W, C3W) were analyzed using the amplified fragment length polymorphism (AFLP) technique compared with each non-irradiated plant. A total of 28 EcoRI and MseI primer combinations were used to screen 8 treatments by the ABI3130 capillary electrophoresis system. Amplified products by 28 primer sets showed 1,679 bands with an average of 51 bands per primer combination. Out of the total bands scored, 1,164 fragments were polymorphic bands, with different alleles existing among the treatments. The cluster analysis was performed using the UPGMA (Unweighted Pair Group Method using Arithmetic) in the computer program NTSYS-pc. In clustery analysis, acute gamma-irradiation showed higher genetic variation compared with chronic gamma-irradiation.

• Journal of Radiation Industry
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• v.2 no.3
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• pp.121-128
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• 2008

Flavanone-3-hydroxylase (F3H) is one of the key enzymes for the biosynthesis of flavonals, anthocyanins, catechins and proanthocyanins. F3H catalyzes the $3{\beta}$-hydroxylation of (2S)-flavonones to form (2R, 3R)-dihydroflavonols. In this report, we isolated a full-length cDNA of RocF3H from black raspberry (Rubus occidentalis L.) using a reverse transcriptase-PCR and rapid amplification of the cDNA ends (RACE)-PCR. The full-length cDNA of RocF3H contains a 1,098 bp open reading frame (ORF) encoding a 365 amino acid protein with a calculated molecular weight of about 41.1 kDa and isoelectric point (pI) of 5.45. The genomic DNA analysis revealed that the RocF3H gene had three exons and two introns. Comparison of the deduced amino acid sequence of the RocF3H with other F3Hs revealed that the protein is highly homologous with various plant species. The conserved amino acids ligating the ferrous iron and the residues participating in the 2-oxoglutarate binding (R-X-S) were found in RocF3H at the similar positions to other F3Hs. Southern blot analysis indicated that RocF3H exist a multi-gene family. The isolation of RocF3H gene will be helpful to further study the role of F3H gene in the biosynthesis of flavonoids in R. occidnetalis.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.17 no.2
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• pp.139-165
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• 2019

The current status and prospect of decommissioning technology development at KAERI are reviewed here. Specifically, this review focuses on four key technologies: decontamination, remote dismantling, decommissioning waste treatments, and site remediation. The decontamination technologies described are component decontamination and system decontamination. A cutting method and a remote handling method together with a decommissioning simulation are described as remote dismantling technologies. Although there are various types of radioactive waste generated by decommissioning activities, this review focuses on the major types of waste, such as metal waste, concrete waste, and soil waste together with certain special types, such as high-level and high-salt liquid waste, organic mixed waste, and uranium complex waste, which are known to be difficult to treat. Finally, in a site remediation technology review, a measurement and safety evaluation related to site reuse and a site remediation technique are described.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.25 no.10
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• pp.1302-1309
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• 2021

Recently, the Korean Government announced the Korean New Deal as a national development strategy to overcome the economic recession from the pandemic crisis and lead the global action against structural changes. In the Korean New Deal, the Green New Deal related with the energy aims to achieve net-zero emissions and accelerates the transition towards a low-carbon and green economy. To this end, the government plans to promote an increased use of renewable energy in the society at large. This paper introduces a binary power generation using unused low-grade thermal energy to accelerate the transition towards a low-carbon and green economy and examines a control system based on Neural Network which is capable maintenance at low-cost by an unmanned automated operation in actual power generation environment. It is expected that the realization of binary power generation accelerates introduction of renewable energy along with solar and wind power.

• Nuclear Engineering and Technology
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• v.55 no.6
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• pp.1974-1987
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• 2023

The load-following operation of small modular reactors (SMRs) requires accurate prediction of transient behaviors that can occur in the balance of plants (BOP) and the nuclear steam supply system (NSSS). However, 1-D thermal-hydraulics analysis codes developed for safety and performance analysis have conventionally excluded the BOP from the simulation by assuming ideal boundary conditions for the main steam and feed water (MS/FW) systems, i.e., an open loop. In this study, we introduced a lumped model of BOP fluid system and coupled it with NSSS without any ideal boundary conditions, i.e., in a closed loop. Various methods for coupling boundary conditions at MS/FW were tested to validate their combination in terms of minimizing numerical instability, which mainly arises from the coupled boundaries. The method exhibiting the best performance was selected and applied to a transient simulation of an integrated NSSS and BOP system of a SMART. For a transient event with core power change of 100-20-100%, the simulation exhibited numerical stability throughout the system without any significant perturbation of thermal-hydraulic parameters. Thus, the introduced boundary-condition coupling method and BOP fluid system model can expectedly be employed for the transient simulation and performance analysis of SMRs requiring daily load-following operations.

• Nuclear Engineering and Technology
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• v.27 no.4
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• pp.571-581
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• 1995

The generation, verification and validation of Emergency Procedures for Nuclear Power Plant is a difficult and complex process. Atomic Energy Control Board(AECB) requires that emergency procedure and plan be produced before obtaining the Operating License, that is, detailed plans and procedures to handle emergency situations for both on-site actions and off-site actions be developed. In this report Emergency Operating Procedures Standard for Canadian Nuclear Utilities which makes reference to U. S. practices and the current direction of emergency procedures for CAN-DU reactors are reviewed and compared based on scope(events covered), methodology (event-oriented or symptom-oriented or hybrid) and format(method of presentation) preponderantly, and an attempt is made to integrate these procedures and as a result the recommended strategy for Wolsong unit 2, 3, & 4 is presented as event-specific procedures, generic procedures(when event is not diagnosed) and whose format is combination of logic diagram and text.

• Clean Technology
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• v.19 no.2
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• pp.156-164
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• 2013

In this study, we analyzed the operational characteristics of a 0.25 MW methanation pilot plant. Isothermal reactor controled the heat released from methanation reaction by saturated water in shell side. Methanation process consisting of isothermal reactor and adiabatic reactor had advantages with no recycle compressor and more less reactors compared with methanation process with only adiabatic reactors. In case that $H_2$/CO ratio of syngas was under 3, carbon deposition occurred on catalyst in tube side of isothermal reactor and the pressure of reactors increased. In case that $H_2$/CO ratio was maintained around 3, no carbon deposition on catalyst in tube side of isothermal reactor was found by monitoring the differential pressure of reactors and by measuring the differential pressure of several of tubes filled with catalyst before and after operating. It was shown that CO conversion and $CH_4$selectivity were over 99, 97%, respectively, and the maximum $CH_4$productivity was $695ml/h{\cdot}g-cat$.