• 대한화장품학회지
• /
• 제34권2호
• /
• pp.101-107
• /
• 2008

신선초 추출물 및 그의 분획물의 미백 효과를 알아보기 위하여, B16 멜라노마와 정상 사람 표피 세포를 이용하여 멜라닌 생성에 관련된 다양한 실험을 실시하였다. 70% ethanol 용매로 추출한 신선초 추출물을 연속적으로 분획하여 hexane, ethyl acetate, n-butanol, 물 분획물을 얻었다. 버섯 티로시나제 활성 실험에서 신선초 추출물 및 그의 분획물은 저해 효과를 보이지 않았으나, B16 멜라노마를 이용한 멜라닌 생성 실험에서는 hexane 분획물과 ethyl acetate 분획물에서 억제 효과를 보였다. 세포내 멜라닌 생성의 초기 단계에 작용하는 티로시나제 단백질의 활성을 조사한 결과, hexane 분획($IC_{50}$ < $25{\mu}g/mL$)과 ethyl acetate분획($IC_{50}$ < $5{\mu}g/mL$)은 알부틴($IC_{50}{\leq}250{\mu}g/mL$)과 비교 시 더 낮은 농도에서 현저하게 티로시나제 활성을 저해시켰다. 따라서 신선초 추출물의 미백 작용 기전을 규명하기 위하여, 멜라노 사이트의 mitogen으로 작용하는 endothelin-1(ET-1)과 UVB에 의해 생성되어 염증을 매개하는 interleukin-$1{\alpha}$(IL-$1{\alpha})$의 생성량을 측정하였다. Hexane 및 ethyl acetate 분획물은 IL-$1{\alpha}$의 생성에 영향을 끼치지 못했으나, 표피세포에서 UVB에 의해 생성된 ET-1을 농도에 따라 감소시킴으로써 멜라노사이트의 melanogenesis를 저해하는 것으로 확인되었다. 따라서 신선초 추출물은 미백 효능을 갖는 화장품 소재로써 사용 가능하리라 판단된다.

• 대한핵의학회:학술대회논문집
• /
• 대한핵의학회 2003년도 제42차 춘계학술대회 및 총회
• /
• pp.5-5
• /
• 2003

• 대한생식의학회:학술대회논문집
• /
• 대한불임학회 2004년도 제47차 추계학술대회
• /
• pp.45-45
• /
• 2004

• 대한약학회:학술대회논문집
• /
• 대한약학회 2001년도 Proceedings of International Convention of the Pharmaceutical Society of Korea
• /
• pp.119-123
• /
• 2001

• 한국생물물리학회:학술대회논문집
• /
• 한국생물물리학회 1998년도 학술발표회
• /
• pp.37-37
• /
• 1998

Effects of endothelin-l (ET-l) on contraction, $Ca^{2＋}$ transient and L -type $Ca^{2＋}$ current ( $I_{Ca,L}$) were investigated in single ventricular myocytes isolated from guinea-pig hearts. ET-l at concentrations of 5 and 10 nM produced a biphasic pattern of inotropism： a first decrease in contraction by 34.4$\pm$2.5 ％ of the control followed by a sustained increase in contraction by 66.6$\pm$8.4% (mean$\pm$S.E.M., n=9).(omitted)

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2005년도 생물공학의 동향(XVII)
• /
• pp.879-879
• /
• 2005

• 한국자원식물학회지
• /
• 제35권6호
• /
• pp.713-719
• /
• 2022

Platycodon grandiflorum and Glycyrrhiza uralensis contain several bioactive compounds, such as saponin, oleanolic acid, and flavone. P. grandiflorum and G. uralensis have traditionally been used to treat disorders related to blood pressure, diabetes, and counteracting poison, and they have antinociceptive and antiinflammatory properties. However, the validity of complex saponin's vasodilatory effect has not been scientifically investigated. Therefore, this study explores the vasodilatory effect of complex saponin extracted from P. grandiflorum and G. uralensis mixture extract on rabbit carotid arteries. To this end, arterial rings with intact or damaged endothelium were used in an organ bath experiment and contracted by endothelin. Complex saponins, the major active constituents of P. grandiflorum and G. uralensis mixture extract, exhibited a moderate vasodilatory effect on the rabbit's basilar arteries. Therefore, treatment with complex saponin extracted from P. grandiflorum and G. uralensis mixture extract may selectively accelerate cerebral blood flow through basilar arterial dilation. Overall, the findings suggest that the extracted complex saponins can serve as vasodilator sources.

• The Korean Journal of Physiology and Pharmacology
• /
• 제7권6호
• /
• pp.311-316
• /
• 2003

Endothelins secreted from keratinocytes are intrinsic mitogens and melanogens of human melanocytes in UVB-induced hyperpigmentation. To elucidate the cellular mechanism of antimelanogenic activity of bamboo extract, the effects of three ingredients of bamboo extract on endothelin 1 (ET-1)-induced $Ca^{2+}$ mobilization were investigated in cultured human melanocytes. ET-1 receptors in human melanocytes were characterized by using specific antagonist, and ET-1 was found to increase intracellular $Ca^{2+}$ concentration ($[Ca^{2+}]_i$) by activating ET-B receptor. SM709 (1,2-O-diferulyl-glycerol), an ingredient of bamboo extract, inhibited ET-1-induced $[Ca^{2+}]_i$ increase in a concentration- and time-dependent manner, although another ingredients SM707 and SM708 had no effect on ET-1-induced $[Ca^{2+}]_i$ increase in human melanocytes. SM709 ($100{\mu}M$), however, did not affect $[Ca^{2+}]_i$ increase induced by thapsigargin and caffeine, suggesting that SM709 has no effect on the $Ca^{2+}$ store in melanocytes. Furthermore, SM709 did not affect $[Ca^{2+}]_i$ increase induced by LPA or ATP, known as G protein-mediated PLC activators like ET-1. Taken together, it is suggested that SM709 antagonizes ET-1-induced transmembrane signaling through ET-B receptor, which maybe a possible underlying mechanism of antimelanogenic activity of bamboo extract in human melanocytes.

• The Korean Journal of Physiology and Pharmacology
• /
• 제1권5호
• /
• pp.565-572
• /
• 1997

In the freshly isolated rat nephron, the effect of endothelin-1, -2 and -3 (ET-1, -2 and -3) on cytosolic free calcium concentration ($[Ca^{2+}]_i$) was determined using the fluorescent indicator Fura-2/AM. $[Ca^{2+}]_i$ increase was investigated in 9 parts of the single nephron including glomerulus (Glm), $S_1,\;S_2,\;S_3$, cortical and medullary thick ascending limb and cortical (CCT) and outer medullary collecting tubule (OMCT). Endothelins increased $[Ca^{2+}]_i$ in Glm (ET-1; $127{\pm}17%$, ET-2; $93{\pm}5%$, ET-3; $169{\pm}17%$), CCT (ET-1; $30{\pm}6%$, ET-2; $38{\pm}19%$, ET-3; $158{\pm}18%$) and OMCT (ET-1; $197{\pm}11%$, ET-2; $195{\pm}11%$, ET-3; $215{\pm}37%$) at 10-7 M. In OMCT, ET-1 and ET-2 increased $[Ca^{2+}]_i$ in a dose-dependent manner ($10^{-10}{\sim}10^{-6}$ M). To the contrary, ET-3-induced $[Ca^{2+}]_i$ rise was begun from $10^{-12}$ M. BQ-123Na, an antagonist of ETA receptor, at $10^{-4}$ M inhibited about 30% of $[Ca^{2+}]_i$ rise induced by ET-1 and -3. Binding experiments using $[^{125}I]ET-3$ showed the existence of $ET_B$ receptor in OMCT. This binding was replaced by ET-1, ET-2 or ET-3 by the almost same degree but not by angiotensin II or vasopressin.

• Animal cells and systems
• /
• 제4권1호
• /
• pp.63-70
• /
• 2000

The distribution of receptor subtypes for endothelin (ET) in the kidney of the freshwater turtle, Amyda japonica, was examined by quantitative in vitro receptor autoradiography using iodinatd mammalian type ET-1 ($^125$/I-ET-1)as a radiolabeled ligand. Specific $^125$/I-ET-1 bindings were localized to renal tubules, renal arteries and ureter with binding densities of 111.21 $\pm$ 19.14, 182.13$\pm$10.57 and 219.46$\pm$12.83 amol/$mm^2$. respectively. Binding dissociation constants in renal tubules, renal arteries and ureter were 1.05 $\pm$ 0.63, 2.03 $\pm$0.56 and 1.70$\pm$0.47nM, respectively. Receptor subtypes for ET in the kidney were characterized by competition with BQ 123 and BQ 788 as specific antagonists for ET receptors, type A (ET$_A$ ), and type B (ET$_B$) subtypes, respectively. Specific $^125$/I-ET-1 bindings in renal arteries and ureter were potently inhibited by BQ 123 in a dose-dependent manner, whereas BQ 788 was not in competing for specific $^125$/I-ET-1 bindings in this structure. However, specific $^125$/I-ET-1 bindings in renal tubules were inhibited more potently by BQ 788. Therefore, these results indicate that specific ET receptors are localized in renal tubules, renal arteries and the ureter of the freshwater turtle. Results also suggest that the predominant ET receptor subtypes are like the ETA receptor in renal arteries and ureter, and like the ET/$_A$ receptor in the renal tubule.