• Electronics and Telecommunications Trends
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• v.34 no.1
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• pp.75-85
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• 2019

Quantum technology is undergoing a revolution. Theoretically, strange phenomena of quantum mechanics, such as superposition and entanglement, can enable high-performance computing, unconditionally secure communication, and high-precision sensing. Such theoretical possibilities have been examined in the last few decades. The goal now is to apply these quantum advantages to daily life. Europe, where quantum mechanics was born a 100 years ago, is struggling to be placed at the front of this quantum revolution. Thus, the European Commission has decided to invest 1 billion EUR over 10 years and has initiated the ramp-up phase with 20 projects in the fields of communication, simulation, sensing and metrology, computing, and fundamental science. This program, approved by the European Commission, is called the "Quantum Technology Flagship" program. Its first objective is to consolidate and expand European scientific leadership and excellence in quantum research. Its second objective is to kick-start a competitive European industry in quantum technology and develop future global industrial leaders. Its final objective is to make Europe a dynamic and attractive region for innovative and collaborative research and business in quantum technology. This program also trains next-generation quantum engineers to achieve a world-leading position in quantum technology. However, the most important principle of this program is to realize quantum technology and introduce it to the market. To this end, the program emphasizes that academic institutes and industries in Europe have to collaborate to research and develop quantum technology. They believe that without commercialization, no technology can be developed to its full potential. In this study, we review the strategy of the Quantum Europe Flagship program and the 20 projects of the ramp-up phase.

• Journal of the Korea Computer Industry Society
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• v.7 no.3
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• pp.245-252
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• 2006

Estrangement hierarchical by bipolarization is deepened and time space that social welfare by graying corresponds great so. Specially, is real condition that indifference by patient's increase which is solitary life string is come to involve by social Voblem. Together, Jaetaek bone fracture patient's ratio is zooming. <중략>Supersonic waves operation frequency used on both end because do 1mHz, 1.3mHz, supersonic waves origination that have 1.5mHz's Piezo-ceramic crystal tranducer material each 4 premature senilitys in this research, and outside diameter according to impedance and Phase d used Gakgak4mm, 5.4mm, Dukke0.5mm, transformer deuce of length 70mm. Manufactured, and investigated supersonic waves distribution chart by capacity 50m W. Supersonic waves used by diagnosis mainly but is seen to become convenient medical treatment mounting in bone fracture patient's treatment if supplement clinically. If supplement system furthermore, is going to apply to osteoporosis patient, and this research tried to design poetic theme width directly and study rain standardization special qualify and approach basic form because do modelling.

• International conference on construction engineering and project management
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• 2020.12a
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• pp.201-206
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• 2020

Diversification of project delivery methods (PDM) under ever-changing construction business environment has significantly changed the role of project participants. Active efforts to effectively sharing the roles and responsibilities have been observed in the project management offices (PMOs) among owner/operator organizations as well as engineering, procurement, construction and maintenance (EPCM) firms. In order for being effective in a holistic way throughout the project life-cycle, a PMO needs to have 'adequate management skills' as well as 'essential technical capabilities' in cooperating with many different participants. One of the well-known examples of the PMO's tool to support these skills and capabilities is the effective 'work packaging (WP)' that serves as a common basis integrating all relevant information in a structured manner. In an attempt to enhance the construction productivity, the concept of 'advanced work packing (AWP)' has been introduced by Construction Industry Institute (CII). The AWP enables productivity to be improved by early planning of construction packages in the design phase "with the end in mind". The purpose of this study is to identify and evaluate the 'variables' of advanced work packing (AWP) for life-cycle information integration. Firstly, an extended concept of advanced WP based on the CII AWP was defined in order to comprehend many different issues of business functions (e.g. cost, schedule, quality, etc.). A structured list of major components and variables of AWP were then identified and examined for practical viability with real-world examples. Strategic fits and managerial effectiveness were stressed throughout the analyses. Findings, implications and lessons learned are briefly discussed as well.

• Journal of Animal Reproduction and Biotechnology
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• v.38 no.3
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• pp.99-108
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• 2023

Background: Efficient gene editing technology is needed for successful knock-in. Homologous recombination (HR) is a major double-strand break repair pathway that can be utilized for accurately inserting foreign genes into the genome. HR occurs during the S/G2 phase, and the DNA mismatch repair (MMR) pathway is inextricably linked to HR to maintain HR fidelity. This study was conducted to investigate the effect of inhibiting MMR-related genes using CdCl₂, an MMR-related gene inhibitor, on HR efficiency in HC11 cells. Methods: The mRNA and protein expression levels of MMR-related genes (Msh2, Msh3, Msh6, Mlh1, Pms2), the HR-related gene Rad51, and the NHEJ-related gene DNA Ligase IV were assessed in HC11 cells treated with 10 μM of CdCl₂ for 48 hours. In addition, HC11 cells were transfected with a CRISPR/sgRNA expression vector and a knock-in vector targeting Exon3 of the mouse-beta casein locus, and treated with 10 μM cadmium for 48 hours. The knock-in efficiency was monitored through PCR. Results: The treatment of HC11 cells with a high-dose of CdCl₂ decreased the mRNA expression of the HR-related gene Rad51 in HC11 cells. In addition, the inhibition of MMR-related genes through CdCl₂ treatment did not lead to an increase in knock-in efficiency. Conclusions: The inhibition of MMR-related gene expression through high-dose CdCl₂ treatment reduces the expression of the HR-related gene Rad51, which is active during recombination. Therefore, it was determined that CdCl₂ is an inappropriate compound for improving HR efficiency.

• Journal of Life Science
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• v.25 no.7
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• pp.780-785
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• 2015

Dietary intake and bioavailability of phorotannins in abalone was investigated after feeding with the phlorotannin-rich brown seaweed Ecklonia stolonifera after 4 days starvation. Reverse-phase high-performance liquid chromatography (RP-HPLC) affords isolation and quantification of the major phlorotannins of 7-phloroeckol and eckol, which were identified by mass spectrometry and nuclear magnetic resonance. Abalone growth and feed consumption rates were similar when fed either with the E. stolonifera or the common feed seaweed Saccharina japonica for 20 days. Throughout the feeding period, 7-phloroeckolol was accumulated in the abalone flesh tissue up to an average of 0.58±0.13 mg/g dry weight after 6 days. Eckol was reached to 0.25±0.05 mg/g dry tissue after 6 days, and maintained the level until end of feeding period. By feeding S. japonica as a control, no phlorotannins were detected in the abalone tissues. Both of the abalone, fed with E. stolonifera or S. japonica, had enzymes that decomposed 7-phloroeckol and eckol in muscle tissues, with similar degradation rates of −0.05 or less and −0.05 mg/ml/hr, respectively. Phlorotannins were reduced by constitutive enzymes in abalone tissues. Therefore, value-added abalone containing bioactive phlorotannins can be produced by simply changing the feed to the phlorotannin-rich brown seaweed E. stolonifera 6 days before harvest.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.4
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• pp.556-562
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• 2007

A total of 126 crossbred weanling pigs (average body weight of $6.3{\pm}0.3$ kg) were used to investigate the effect of chito-oligosaccharide (COS) on growth performance, nutrient digestibility, pH of gastro-intestinal tract (GI), intestinal and fecal microflora of young piglets. Pigs were allocated to three dietary treatments based on body weight and gender in a single factorial arrangement. Treatments were control (No COS), T1 (0.2% COS during starter (6-13 kg) and 0.1% COS during grower (13-30 kg) phases, and T2 (0.4% COS during starter (6-13 kg) and 0.3% COS during grower (13-30 kg) phases, respectively. Each treatment had 3 replicates and 14 pigs were raised in each pen. COS is a low molecular weight water-soluble chitosan that can be obtained from chitin of the crab shell after deacetylation with concentrated sodium hydroxide at high temperature and then further decomposition by chitosanase enzyme in the presence of ascorbic acid. For the starter and grower periods, there were no significant differences (p>0.05) in average daily gain (ADG) and feed to gain ratio among treatments. However, during the overall period (6-30 kg), T2 showed better (p<0.05) feed to gain ratio than other treatments. A digestibility study was conducted at the end of grower phase which showed improvement (p<0.05) in DM and crude fat digestibility in T2 over the control. At 25 kg body weight, 6 pigs per treatment (2 per replicate) were sacrificed to determine the effect of diets on pH and microbial count at different sections of the GI tract. The pH of the cecal contents in pigs fed 0.1% COS was higher (p<0.05) than in the other treatments. Total anaerobic bacterial number increased from cecum to rectum in all treatments. The weekly total bacterial counts showed higher (p<0.05) in feces of pigs fed COS than that of untreated pigs at the $8^{th}$ week. The number of fecal E. coli in untreated pigs at $4^{th}$ wk was 7.35 log CFU/g compared to 6.71 and 6.54 log CFU/g in 0.1 and 0.3% COS-treated pigs, respectively. Similarly, at $8^{th}$ wk, fecal clostridium spp. were lower in pigs fed 0.3% COS (5.43 log CFU/g) than in untreated pigs (6.26 log CFU/g). In conclusion, these results indicated that chito-oligosaccharide could improve feed efficiency in young pigs and inhibited the growth of harmful bacteria.

• Journal of Animal Science and Technology
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• v.64 no.1
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• pp.10-22
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• 2022

We studied the effects of Saccharomyces cerevisiae boulardii CNCM I-1079 (LSB) supplemented to lactating sows on reproductive traits and farrowing duration and to piglets from day 7 of life on post-weaning performance and IgG concentration. Ninety-six Landrace × Yorkshire sows started the trial 5 days before the expected farrowing date. Sows were distributed into 2 groups according to parity number and backfat thickness: control (CON: regular lactation diet) and LSB (CON + LSB at 2 × 10⁹ colony forming units [CFU]/kg of feed). Seven days after birth, litters were randomly selected from each group and supplemented creep feed with or without LSB at 2 × 10⁹ CFU/kg. At weaning, piglets from CON sows were shifted to a commercial farm and allocated to 14 pens in groups of 25 piglets/pen according to the creep feed supplemented during lactation. Piglets followed a 3-phase feeding program: creep, pre-starter and starter, with or without LSB at 2 × 10⁹ CFU/kg LSB in creep and pre-starter, and 1 × 10⁹ CFU/kg LSB in starter. The piglets were vaccinated against classical swine fever on days 41 and 72 of life. One day before each vaccination and at the end of the trial, blood samples were collected from 15 randomly selected piglets per treatment and assessed for total IgG. Supplemented sows with non-supplemented litters displayed the lowest backfat thickness loss during lactation (p < 0.05). The LSB supplementation shortened farrowing duration (p < 0.05) and increased feed intake (p < 0.05) during the first week of lactation. The LSB-fed piglets were heavier at the end of creep (p < 0.05), pre-starter (p < 0.05), and the trial (p < 0.05); grew faster during creep (p < 0.05), starter (p < 0.05), and overall (p < 0.05); and displayed an improved feed conversion ratio during creep (p < 0.05). Total IgG content was higher at days 40 (p < 0.05) and 71 (p < 0.05) in LSB-fed piglets. We conclude that supplementing sows with Saccharomyces cerevisiae boulardii CNCM I-1079 from late gestation until weaning shortens farrowing duration, increases feed intake, and minimizes backfat losses during lactation. When supplemented to piglet diet, post-weaning performance is improved. This improvement observed could be linked to a better immune status, as suggested by the higher IgG.

• Journal of Life Science
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• v.17 no.2 s.82
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• pp.272-278
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• 2007

Proteases are enzymes that break peptide bonds between amino acids of other proteins and occupy a crucial position with respect to their applications in both physiological and commercial fields. In order to screen new source of protease, bacteria producing extracellular proteases at low temperature were isolated from deep sea water of East Sea, Korea. A bacterium showing the best growth rate and production of an extracellular protease at low temperature was designated HJ 47. The DNA sequence analysis of the 16S rRNA gene, phenotypic tests and morphology led to the placement of this organism in the genus Pseudoalteromonas. Although maximal growth was observed at $37^{\circ}C$, enzyme production per culture time was maximum at $20^{\circ}C$. At this temperature, extracellluar protease production was detected from the end of the exponential phage to stationary phase, and maximal at 15 hours after initial production. The optimum temperature and pH of the protease were found to be $35^{\circ}C$ and 8.

• Journal of Microbiology and Biotechnology
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• v.26 no.4
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• pp.790-798
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• 2016

Chlamydiae, obligate intracellular bacteria, are associated with a variety of human diseases. The chlamydial life cycle undergoes a biphasic development: replicative reticulate bodies (RBs) phase and infectious elementary bodies (EBs) phase. At the end of the chlamydial intracellular life cycle, EBs have to be released to the surrounded cells. Therefore, the interactions between Chlamydiae and cell death pathways could greatly influence the outcomes of Chlamydia infection. However, the underlying molecular mechanisms remain elusive. Here, we investigated host cell death after Chlamydia infection in vitro, in L929 cells, and showed that Chlamydia infection induces cell necrosis, as detected by the propidium iodide (PI)-Annexin V double-staining flow-cytometric assay and Lactate dehydrogenase (LDH) release assay. The production of reactive oxygen species (ROS), an important factor in induction of necrosis, was increased after Chlamydia infection, and inhibition of ROS with specific pharmacological inhibitors, diphenylene iodonium (DPI) or butylated hydroxyanisole (BHA), led to significant suppression of necrosis. Interestingly, live-cell imaging revealed that Chlamydia infection induced lysosome membrane permeabilization (LMP). When an inhibitor upstream of LMP, CA-074-Me, was added to cells, the production of ROS was reduced with concomitant inhibition of necrosis. Taken together, our results indicate that Chlamydia infection elicits the production of ROS, which is dependent on LMP at least partially, followed by induction of host-cell necrosis. To our best knowledge, this is the first live-cell-imaging observation of LMP post Chlamydia infection and report on the link of LMP to ROS to necrosis during Chlamydia infection.

• Korean Journal of Construction Engineering and Management
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• v.15 no.4
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• pp.119-127
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• 2014

This study aims to determine whether or not the input output life cycle assessment (I-O LCA) model can be used to assess the carbon dioxide (CO2) emission of buildings in initial planning phase. To ensure this end, this study proposed I-O LCA model which is the simplified LCA model and Hybrid LCA model which is the detailed LCA model, and then assessed and compared the CO2 emission of six case projects (three apartment complexes and three educational facilities) using the two LCA model. The results of the case study showed that the CO2 emissions assessed by the I-O LCA is significantly similar to the CO2 emission assessed by the Hybrid LCA model. The similarity of results from both LCA models was 78.2-86.3% in apartment complexes and 59.9-84.8% in educational facilities. However, the CO2 emissions from I-O LCA model were smaller than the CO2 emissions from Hybrid LCA model in case study. Nevertheless, the case study showed that the I-O LCA model was capable of assessing the CO2 emission of buildings quite appropriately although the I-O LCA model is the simplified LCA model which considers only the construction cost. The I-O LCA model is expected to be a useful tool for assessing the CO2 emission of buildings in initial planning phase.