• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.2
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• pp.126-132
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• 2010

A high sugar rice cultivar 'Danmi' which has been recently developed and released in the Functional Crop Resource Development Division, Department of Functional Crop, National Institute of Crop Science, has high glucose and sucrose contents but has a poor seed development. This defect poses some problem in ensuring stable seedling establishment in the nursery bed. We examined the germination and seedling emergence characteristics of 'Danmi' and determined an optimum seeding rate for seedling establishment. 'Danmi' seeds has a light 1,000-grain weight of 18.2g compared with Ilmibyeo's 26.7g. The 97.4% of 'Danmi' seeds has specific gravity below 1.0 while only 2.0% of seeds was fully developed with specific gravity above 1.12. However, the reverse is true in the Ilmibyeo seed used as a control cultivar. Although the seed germination of Danmi was 89-91% regardless of seed specific gravity but at a slower rate than Ilmibyeo regardless of water soaking temperatures of $20-30^{\circ}C$ because of high seed amount of specific gravity below 1.0 in 'Danmi'. It took 2～4 days longer of seed soaking in the Danmi seeds than Ilmibyeo before germination. 'Danmi' has low normal seedling emergence rate than Ilmibyeo in the seedbed soil due to high percentage of abnormal seedlings like stunting and incomplete growth. Normal seedling emergence rate of 'Danmi' in the seedbed was 62.6～64.9% for 10-day old seedling and 83.5～86.7% for 30-day old seedling which is lower by 29.6～30.0% and 11.3～12%, respectively than Ilmibyeo. Although 'Danmi' has low normal seedling emergence rate, but it has greater seed number per weight basis. Therefore, based on the normal seedling number per unit area of Ilmibyeo(control cultivar) for seedling rate of 10-day and 30-day old seedlings, the recommended seeding rate of 'Danmi' for transplanting rice is 220g seeds for 10-day old seedling and 130g for 30-day old seedling per nursery box, which is equivalent to 1.5 times seed volumes of Ilmibyeo.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.2
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• pp.144-151
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• 2019

The establishment rate of foxtail millet seeds is greatly affected by soil environment conditions. To enhance germination viability and stable production of foxtail millet seeds, it is important to select seeds with a high density. Therefore, this study tested the selection of high-quality seeds using salt solution (specific gravity: 1.000, 1.005, 1.010, 1.015, 1.020, 1.025, 1.030, 1.035, and $1.040g\;L^{-1}$) and investigated their germination rates, percentages of emergence, and seeding quality. In this study, three varieties were tested: 'Hwanggeum', 'Samdachal', and 'Kyeongkwan1'. The thousand seed weight of all three varieties increased proportionally with specific gravity. The highest thousand seed weight was observed at the selection with $1.040g\;L^{-1}$ specific gravity, which also had the highest germination rate of 88.3%, 86.7%, and 90.6%, for 'Hwanggeum'; 'Samdachal'; and 'Kyeongkwan1', respectively. The results of seeding quality indicated that higher the specific gravity during selection, the higher were the values of plant growth (plant height, leaf length, stem diameter, root length, root weight, and stem weight). All the three varieties were found to be longer or heavier for seeds selected at the specific gravity of $1.040g\;L^{-1}$. The packing germination viability investigation found that higher the specific gravity for selection of seeds, the higher was the percentage of emergence (PE) and the emergence rate index (ERI). The PE was the highest for seeds selected at $1.040g\;L^{-1}$ specific gravity (85.3, 83.0, and 87.0%), and ERI was also as high as $2.82d^{-1}\;m^{-1}$ or more at $1.040g\;L^{-1}$. Selection of seeds with salt solution resulted in high germination viability of foxtail millet. Therefore, sowing seeds selected at $1.040g\;L^{-1}$ specific gravity is expected to help substantially in increased productivity.

• Journal of Environmental Health Sciences
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• v.44 no.2
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• pp.133-142
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• 2018

Objectives: The pathogenic Vibrios genus denotes halophilic bacteria that are distributed in aquatic environments, including both sea and freshwater. Vibrio cholerae, Vibrio vulnificus, and Vibrio parahaemolyticus are the most important species since they can be potent human pathogens and leading causes of septicemia, wound infections, and seafood borne gastroenteritis. The recent emergence of a potential pandemic clone, V. cholera serotype O1 and the cholera outbreak in South Korea in 2016 indicates the importance of consistent surveillance of pathogenic Vibrio genus within coastal areas. Methods: The present study was undertaken to determine where and how vibrios live in the aquatic environment adjacent to coastal areas of South Korea. For this survey, a total of 838 samples were obtained at 35 different sites in South Korean coastal areas during the period from January 2016 to December 2016. Pathogenic vibrios was determined using the real-time PCR method, and its clones were isolated using three selective plating media. We also monitored changes in seawater and atmospheric temperature, salinity, turbidity, and hydrogen ion concentration at the collection points. Results: The total isolation rates of V. vulnificus, V. cholera (non-pathogenic, non-O1, non-O139 serogroups), and V. parahaemolyticus from seawater specimens in 2016 were 14.2, 13.48, and 67.06%, respectively. Conclusions: The isolation rates of pathogenic vibrios genus showed a positive correlation with temperature of seawater and atmosphere but were negatively correlated with salinity and turbidity.

• Mycobiology
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• v.39 no.3
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• pp.206-218
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• 2011

The seed-borne mycoflora of sorghum and foxtail millet collected from different growing areas in South Korea were isolated and taxonomically identified using dry inspection, standard blotter and the agar plate method. We investigated the in vitro and in vitro germination rates of disinfected and non-disinfected seeds of sorghum and foxtail millet using sterilized and unsterilized soil. The percent recovery of seed-borne mycoflora from the seed components of sorghum and foxtail millet seeds was determined and an infection experiment using the dominant species was evaluated for seedling emergence and mortality. A higher number of seed-borne fungi was observed in sorghum compared to that of foxtail millet. Eighteen fungal genera with 34 fungal species were identified from the seeds of sorghum and 13 genera with 22 species were identified from the seeds of foxtail millet. Five dominant species such as Alternaria alternata, Aspergillus flavus, Curvularia lunata, Fusarium moniliforme and Phoma sp. were recorded as seed-borne mycoflora in sorghum and 4 dominant species (Alternaria alternata, Aspergillus flavus, Curvularia lunata, Fusarium moniliforme) were observed in foxtail millet. The in vitro and in vitro germination rates were higher using disinfected seeds and sterilized soil. More seed-borne fungi were recovered from the pericarp compared to the endosperm and seed embryo. The percent recovery of seed-borne fungi ranged from 2.22% to 60.0%, and Alternaria alternata, Curvularia lunata and 4 species of Fusarium were isolated from the endosperm and embryo of sorghum and foxtail millet. Inoculation of the dominant seed-borne fungi showed considerable mortality of seedlings. All the transmitted seed-borne fungi might well be a primary source of infection of sorghum and foxtail millet crops.

• Journal of Radiation Industry
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• v.8 no.2
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• pp.111-121
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• 2014

This study was aimed to select useful mutants of rapeseed (Brassica napus L.) and leaf mustard (Brassica juncea L.), the seeds of three lines S-14, S-27, and S-28 were treated with gamma-ray and EMS. The optimum ranges of gamma-ray dose and EMS concentration to enlarge the characteristic morphological variations were also separately investigated. The survival rates of S-28 only linearly decreased with increasing the gamma-ray dose. The overall growth parameters decreased of gamma-ray dose in all three lines of S-14, S-27, and S-28. The reduction dosage 50 of gamma-ray was identified as 1,200 Gy for S-14 leaf mustard, while those of S-27 and S-28 rapeseed lines were appeared as same 1,000 Gy. The emergence rates of S-14 and S-27 showed no significant differences by EMS treatment, while the growth of all three lines were significantly decreased. The reduction concentration 50 in S-14 could not be determined, demonstrating that this leaf mustard line is presumably insensitive to mutagenic EMS, while those in S-27 and S-28 were identified as 3.0 and 2.5%, respectively, showing that these rapeseed lines possess higher sensitivity to EMS than S-14. Various morphological characteristics of $M_1$ generation obtained from mutagen treatment were elaborately investigated for further maintenance of $M_2$ generation. In $M_2$ generation variants showing short stem, yellow color in seed coat, chlorophyll deficiencies in leaf or pod, abnormal flower color were selected as potentially useful mutants for breeding.

• Annals of Laboratory Medicine
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• v.38 no.6
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• pp.545-554
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• 2018

Background: The increasing morbidity and mortality rates associated with Acinetobacter baumannii are due to the emergence of drug resistance and the limited treatment options. We compared characteristics of colistin-resistant Acinetobacter baumannii (CR-AB) clinical isolates recovered from patients with and without prior colistin treatment. We assessed whether prior colistin treatment affects the resistance mechanism of CR-AB isolates, mortality rates, and clinical characteristics. Additionally, a proper method for identifying CR-AB was determined. Methods: We collected 36 non-duplicate CR-AB clinical isolates resistant to colistin. Antimicrobial susceptibility testing, Sanger sequencing analysis, molecular typing, lipid A structure analysis, and in vitro synergy testing were performed. Eleven colistin-susceptible AB isolates were used as controls. Results: Despite no differences in clinical characteristics between patients with and without prior colistin treatment, resistance-causing genetic mutations were more frequent in isolates from colistin-treated patients. Distinct mutations were overlooked via the Sanger sequencing method, perhaps because of a masking effect by the colistin-susceptible AB subpopulation of CR-AB isolates lacking genetic mutations. However, modified lipid A analysis revealed colistin resistance peaks, despite the population heterogeneity, and peak levels were significantly different between the groups. Conclusions: Although prior colistin use did not induce clinical or susceptibility differences, we demonstrated that identification of CR-AB by sequencing is insufficient. We propose that population heterogeneity has a masking effect, especially in colistin non-treated patients; therefore, accurate testing methods reflecting physiological alterations of the bacteria, such as phosphoethanolamine-modified lipid A identification by matrix-assisted laser desorption ionization-time of flight, should be employed.

• Korean Journal of Medicinal Crop Science
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• v.27 no.3
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• pp.167-172
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• 2019

Background: Angelica acutiloba Kitagawa belongs to the flowering plant family Apiaceae. Its young leaves are consumed as a vegetable, and its roots have medicinal properties. This study was conducted to analyze the seed germination characteristics of A. acutiloba according to its ripening stages. Methods and Results: The seeds were harvested from a research farm managed by the Department of Herbal Crop Research, Rural Development Administration in 2018 and were divided into six groups according to their specific gravities. In particular, we studied the effect of harvesting periods and umbel orders on seed characteristics. The results showed that the rates of germination, emergence, and early growth improved as the specific gravity of the seeds increased. In addition, the germination rates of the seeds harvested in mid July and early August were significantly higher than those harvested in the other seasons, and the seeds obtained from the first floret had the greatest mass and weight. Conclusions: In this study, we demonstrated that it is possible to improve the germination rate by appropriate selection of seeds and harvesting period both of which are closely related to seed maturity.

• Structural Engineering and Mechanics
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• v.89 no.6
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• pp.617-626
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• 2024

The study investigated the behavior of plain and fibered Ultra-High Performance Concrete (UHPC) beams under varying loading conditions using integrated analysis of the flexure and acoustic emission tests. The loading rate of testing is -0.25 -2 mm/min. It is observed that on increasing loading rate, flexural strength increases, and toughness decreases. The acoustic emission testing revealed that higher loading rates accelerate crack propagation. Fiber effect and matrix cracking are identified as significant contributors to the release of acoustic emission energy, with fiber rupture/failure and matrix cracking showing rate-dependent behavior. Crack classification analysis indicated that the rise angle (RA) value decreased under quasi-static loading. The average frequency (AF) value increased with the loading rate, but this trend reversed under rate-dependent conditions. K-means analysis identified distinct clusters of crack types with unique frequency and duration characteristics at different loading rates. Furthermore, the historic index and signal strength decreased with increasing loading rate after peak capacity, while the severity index increased in the post-peak zone, indicating more severe damage. The sudden rise in the historic index and cumulative signal strength indicates the possibility of several occurrences, such as the emergence of a significant crack, shifts in cracking modes, abrupt failure, or notable fiber debonding/pull-out. Moreover, there is a distinct rise in the number of AE knees corresponding to the increase in loading rate. The crack mapping from acoustic emission testing aligned with observed failure patterns, validating its use in structural health monitoring.

• Weed & Turfgrass Science
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• v.7 no.2
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• pp.111-119
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• 2018

This study was carried out to investigate the differences in germination and growth of Quamoclit coccinea Moench under various temperatures, seeding depths, and levels of shading and soil moisture for effective weed management. Seed dormancy of Q. coccinea Moench was over 1 year; best results were obtained when seeds were soaked in sulfuric acid for 15 minutes in order to break the dormancy. Germination rates of Q. coccinea Moench ranged from 69 to 73% at $25-35^{\circ}C$ and 26% at $15^{\circ}C$. The germination rates ranged from 70-84% at 2, 3, 5, 7, and 8 cm of seeding depths. In addition, the germination rates were 7% and 13% at 12 cm and 15 cm of seeding depths, respectively, and showed normal growth at the both seeding depths. Q. coccinea Moench showed a high germination rate regardless of shading levels, but shoot fresh weight varied depending on the level of shading as follows: 20%>no shading=shading 35%>shading 50%>shading 75%=shading 90%. Q. coccinea Moench did not germinate when soil had a saturation rate of either 30% or 100%. However, 60-83% of seeds germinated with optimal growth when soil had saturation rates of 60% and 80%.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.49-49
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• 2015

Over the past decade, clubroot has emerged as a major constraint to canola (Brassica napus) production in central Alberta, Canada. The number of fields with confirmed P. brassicae infestations in Alberta has increased steadily from 12 in 2003 to nearly 2,000 in 2014. Management of clubroot on canola has focused on sanitization of field equipment, soil amendments to reduce viable pathogen populations, long rotations out of susceptible crops and cropping of resistant cultivars. Clubroot resistance is the most effective and economical method of disease mitigation, but the recent identification of isolated P. brassicae populations with novel virulence phenotypes capable of overcoming resistance in most canola cultivars highlights the variable nature and adaptability of the pathogen. Recent studies have shown slight reductions in pathogen populations through crop rotations, but much more substantial reductions in spore populations in heavily infested areas near field entrances using fumigants such as Vapam (metam-sodium) or Basamid (dazomet). Greenhouse trials showed that seedling emergence, plant height and root weight increased, while primary and secondary infection and disease severity decreased with increased Basamid dosage. However, field trials showed some phytotoxicity. Application of Vapam at rates of 0.4 to $1.6mL\;L^{-1}$ soil resulted in 12-16 fold reductions in clubroot severity and primary and secondary infection. Vapam also was effective in reducing clubroot severity and improving canola seed yield under field conditions. These studies underscore the need for good resistance stewardship and for the integration of multiple products and practices for successful management of clubroot on canola.