• Geomechanics and Engineering
• /
• 제24권5호
• /
• pp.479-490
• /
• 2021

Pre-drainage of groundwater in the roof aquifer by boreholes is the main method for prevention of roof water disaster, and the drop in the water level during the drainage leads to the variation of the local stress in the overlying strata. Based on a multitude of boreholes for groundwater drainage from aquifer above the 1303 mining face of Longyun Coal Mine, theoretical analysis and numerical simulation are used to investigate the local stress variation in the process of borehole drainage. The results show that due to the drop in the water level of the roof aquifer during the drainage, the stress around the borehole gradually evolved. From the center of the borehole to the outside, a stress-relaxed zone, a stress-elevated zone, and a stress-recovered zone are sequentially formed. Along with the expansion of drainage influence, the stress peak in the stress-elevated zone also moves to the outside. When the radius of influence develops to the maximum, the stress peak position no longer moves outward. When the coal mining face advances to the drainage influence range, the abutment pressure in front of the mining face is superimposed with the high local stress around the borehole, which increases the risk of stress concentration. The present study provides a reference for the stress concentration caused by borehole drainage, which can be potentially utilized in the optimal arrangement of drainage boreholes in underground mining.

• The Korean Journal of Physiology and Pharmacology
• /
• 제25권4호
• /
• pp.307-319
• /
• 2021

Dysregulation of the Wnt pathway causes various diseases including cancer, Parkinson's disease, Alzheimer's disease, schizophrenia, osteoporosis, obesity and chronic kidney diseases. The modulation of dysregulated Wnt pathway is absolutely necessary. In the present study, we evaluated the anti-inflammatory effect and the mechanism of action of Wnt-C59, a Wnt signaling inhibitor, in lipopolysaccharide (LPS)-stimulated epithelial cells and macrophage cells. Wnt-C59 showed a dose-dependent anti-inflammatory effect by suppressing the expression of proinflammatory cytokines including IL6, CCL2, IL1A, IL1B, and TNF in LPS-stimulated cells. The dysregulation of the Wnt/β-catenin pathway in LPS stimulated cells was suppressed by WntC59 treatment. The level of β-catenin, the executor protein of Wnt/β-catenin pathway, was elevated by LPS and suppressed by Wnt-C59. Overexpression of β-catenin rescued the suppressive effect of Wnt-C59 on proinflammatory cytokine expression and nuclear factor-kappa B (NF-κB) activity. We found that the interaction between β-catenin and NF-κB, measured by co-immunoprecipitation assay, was elevated by LPS and suppressed by Wnt-C59 treatment. Both NF-κB activity for its target DNA binding and the reporter activity of NF-κB-responsive promoter showed identical patterns with the interaction between β-catenin and NF-κB. Altogether, our findings suggest that the anti-inflammatory effect of Wnt-C59 is mediated by the reduction of the cellular level of β-catenin and the interaction between β-catenin and NF-κB, which results in the suppressions of the NF-κB activity and proinflammatory cytokine expression.

• 대한유전성대사질환학회지
• /
• 제20권2호
• /
• pp.55-62
• /
• 2020

HT-1은 FAH의 돌연변이에 의한 FAH의 결함으로 타이로신 대사의 중간산물인 FAA, SA가 간세포, 신세뇨관 세포에 축적되어 증상을 일으키는 대사이상 질환의 일종이다. 생후 6개월 내에 증상이 발현되는 급성형의 경우 간기능 저하에 따른 간부전, 응고장애, Fanconi 증후군, FAA 축적에 의한 델타-아미노레부릭산 탈수효소의 기능 저하에 의한 포르피린증 유사 증상이 발생한다. 증상이 생후 12개월 이후 발현되는 만성형의 경우 간경화, 간세포암종, 저인산혈증 구루병, 인지기능 저하와 같은 증상이 발생할 수 있다. 본 증례는 생후 20개월 경 간비장비대, 내반슬, 대상산증으로 대사이상질환 감별을 위하여 시행한 탠덤매스 분석에서 특이 소견이 보고되지 않았으나, 57개월에 시행한 복부 초음파 검사에서 간경화, 소변 유기산 분석에서 SA 상승, NGS에서 FAH 돌연변이 c.107T>C (p.Ile 36Thr), c.614T>C (pPhe205Ser)가 확인되어 HT-1이 진단된 증례로, 환아는 현재까지 NTBC 및 단백제한식이를 유지하며 특이 합병증 없이 외래 추적관찰 중이다. HT-1은 혈장 타이로신 농도가 증가하지 않는 경우도 있으며, 만성형의 경우 임상양상이 생후 12개월 이후에 나타나므로 탠덤매스 검사에서 음성 소견이 보고되어도 임상적으로 의심되는 경우 소변 유기산 분석과 같은 추가적 검사가 필요하다. 또한 기존의 탠덤매스 분석을 통하여는 HT-1이 진단되지 않는 경우가 있을 수 있어, 신생아 선별검사에서 혈장 타이로신 농도뿐 아니라 SA 농도도 추가로 확인하는 것의 비용 편익에 대한 추가적 연구가 필요하다.

• Journal of Chest Surgery
• /
• 제35권10호
• /
• pp.730-735
• /
• 2002

횡격막은 횡격막 신경과 동측의 하 흉벽 늑간신경의 지배를 받는다. 상완신경총의 적출 손상을 가진 환자에서 신경총의 일부 신경의 신경이식술에 횡경막 신경의 신경 이식편이 종종 이용된다. 이와 같이 횡격막 신경이 신경이식편으로 사용된 환자에서 횡격막 신경의 탈신경으로 인하여 발생하는 횡격막의 위치 및 운동의 변화를 연구하였다. 대상 및 방법 : 어께의 둔상으로 상완신경총의 적출 손상 때문에 동측의 횡격막 신경으로 근피부신경에 신경이식술을 시행한 13예를 대상으로 하였다. 흉강경 수술방법으로 흉강 내 횡격막 신경을 박리하고 횡격막 바로 위에서 횡격막 신경을 절단하여 경부 절개창을 통해서 외부로 끌어낸 다음 피하 터널을 통해서 동측의 근피부신경에 이식하였다. 엑스선 투시검사와 흉부 엑스선 촬영으로 수술 전후의 횡격막 위치 및 운동 상태를 조사하였다. 결과 : 흉강경을 이용하여 횡격막 신경을 박리 절단하는데 기술적 어려움이나 경미한 합병증도 없었다. 횡격막 신경의 절단 직후에는 횡격막이 평균 1.7 늑간 정도 올라가 있었으나, 엑스선 투시검사에서 흡기시 횡격막의 역행성 운동은 보이지 않았다. 1.5개월 이후의 엑스선 검사에서 횡격막의 위치는 수술 전에 비해 유의한 차이 없을 정도(평균 0.9 늑간 차이; p＝NS로 복원되었다. 횡격막 신경의 절단으로 횡격막의 운동 범위는 탈신경 전에 비해 유의한 차이가 없었다. 결론 : 횡격막 신경의 차단으로 인한 탈신경 후 횡격막의 운동기능은 남아 있었으며 횡격막의 위치는 시간이 경과함에 따라 어느 정도 회복되는 현상을 보였다. 그러나 폐활량이 계속 감소된 소견은 횡격막의 흡기력이 완전히 회복되지 않았음을 추정할 수 있다.

• Nutrition Research and Practice
• /
• 제8권1호
• /
• pp.54-58
• /
• 2014

The liver is vulnerable to alcohol-related injury because it is the primary site of alcohol metabolism. Additionally, a number of potentially dangerous by-products are generated as alcohol is broken down in the liver. However, dietary supplements may prevent or relieve some of alcohol's deleterious effects. Therefore, this study was conducted to evaluate the prophylactic effect of aqueous extract of Sesamum indicum (SI) on ethanol induced toxicity in rats. Male Wistar albino rats were divided into control, ethanol, pre-treatment, simultaneous and post-treatment groups. In the prophylactic experiment, Sesamum indicum, (200 mg/kg body weight) was administered by oral gavage for 28 days; two hours before, simultaneously with or two hours after ethanol exposure. Toxicity was induced by administering 45% ethanol (4.8 g/kg bw) by oral gavage. Lipid peroxidation (TBARS) and reduced glutathione (GSH) levels and catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and gluthathione-S-transferase (GST) activities were then determined in the liver, serum triglyceride (TG) levels, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were monitored and histological examination was carried out. The results revealed that ethanol administration led to significant elevation of TBARS level while depleting in the level of GSH as well as CAT, GPx, SOD and GST activities. Similarly, TG level and ALT and AST activities were elevated. The SI pre-treated group significantly inhibited TBARS, restored GSH level, enhanced CAT, GPx, SOD and GST activities and significantly decreased the elevated level of serum TG, ALT and AST activities. SI treatment (simultaneously with ethanol) exhibited similar effects to those of the SI pre-treated groups, while the SI post-treated group did not show the same protection as the Pre-treated group. S. indicum possesses antioxidant and hepatoprotective properties, that eliminate the deleterious effects of toxic metabolites of ethanol.

• Korean Journal of Radiology
• /
• 제20권5호
• /
• pp.709-718
• /
• 2019

Objective: To investigate the association of myocardial blood flow (MBF) quantified by dynamic computed tomography (CT) myocardial perfusion imaging (MPI) with troponin level and left ventricle (LV) function in patients with ST-segment elevated myocardial infarction (STEMI). Materials and Methods: Thirty-five STEMI patients who successfully had undergone reperfusion treatment within 1 week of their infarction were consecutively enrolled. All patients were referred for dynamic CT-MPI. Serial high-sensitivity troponin T (hs-TnT) levels and left ventricular ejection fraction (LVEF) measured by echocardiography were recorded. Twenty-six patients with 427 segments were included for analysis. Various quantitative parameters derived from dynamic CT-MPI were analyzed to determine if there was a correlation between hs-TnT levels and LVEF on admission and again at the 6-month mark. Results: The mean radiation dose for dynamic CT-MPI was 3.2 ± 1.1 mSv. Infarcted territories had significantly lower MBF (30.5 ± 7.4 mL/min/100 mL versus 73.4 ± 8.1 mL/min/100 mL, p < 0.001) and myocardial blood volume (MBV) (2.8 ± 0.9 mL/100 mL versus 4.2 ± 1.1 mL/100 mL, p = 0.044) compared with those of reference territories. MBF showed the best correlation with the level of peak hs-TnT (r = -0.682, p < 0.001), and MBV showed a moderate correlation with the level of peak hs-TnT (r = -0.437, p = 0.026); however, the other parameters did not show any significant correlation with hs-TnT levels. As for the association with LV function, only MBF was significantly correlated with LVEF at the time of admission (r = 0.469, p = 0.016) and at 6 months (r = 0.585, p = 0.001). Conclusion: MBF quantified by dynamic CT-MPI is significantly inversely correlated with the level of peak hs-TnT. In addition, patients with lower MBF tended to have impaired LV function at the time of their admission and at 6 months.

• 한국공간구조학회논문집
• /
• 제20권2호
• /
• pp.59-68
• /
• 2020

In this paper, the dynamic snapping of the 3-free-nodes spatial truss model was studied. A governing equation was derived considering geometric nonlinearity, and a model with various conditions was analyzed using the fourth order Runge-Kutta method. The dynamic buckling phenomenon was observed in consideration of sensitive changes to the force mode and the initial condition. In addition, the critical load level was analyzed. According to the results of the study, the level of critical buckling load elevated when the shape parameter was high. Parallelly, the same result was caused by the damping term. The sensitive asymmetrical changes showed complex orbits in the phase space, and the critical load level was also becoming lowly. In addition, as the value of damping constant was high, the level of critical load also increases. In particular, the larger the damping constant, the faster it converges to the equilibrium point, and the occurrence of snapping was suppressed.

• 동의신경정신과학회지
• /
• 제19권3호
• /
• pp.101-115
• /
• 2008

Objective: The purpose of this study was to investigate the protective effects of Sam-Jeong-Hwan(SJH) on the animal model of depression induced immobilization stress. Method: The subject were divided into 4 groups(l. normal 2. saline solution administered during immobilization stress treatment 3. SJH of 100mg/kg administered 4. BKJ of 400mg/kg administered). Immobilization stress was treated for 1 hours on day. During 2 days of immobilization stress treatment, they were executed forced swimming test, passive avoidance test, elevated plus maze test. Corticosterone and ACTH in blood were measured. Results: In forced swimming test, SJH of 400mg/kg group showed decreased immobilization. In passive avoidance test, SJH of 400mg/kg group showed increased learning execution. In EPM test, SJH of 400mg/kg group showed decreased anxiety. In locomotor activity test, SJH groups showed significantly increased locomotor activity. Stress group showed significantly increase in serum level of corticosterone, SJH of 400mg/kg group showed decreased serum level of corticosterone. Stress group showed significantly increase in serum level of ACTH, SJH of 400mg/kg group showed decreased serum level of ACTH. Conclusion: These results suggest that Sam-Jeong-Hwan(SJH) is effective in the treatment of depression.

• The Korean Journal of Physiology and Pharmacology
• /
• 제4권4호
• /
• pp.291-299
• /
• 2000

Morphine or butorphanol was continuously infused into cerebroventricle (i.c.v.) with the rate of $26\;nmol/{\mu}l/h$ for 3 days, and the withdrawal from opioid was rendered 7 hrs after the stopping of infusion. The expression of physical dependence produced by these opioids was evaluated by measuring the naloxone-precipitated withdrawal signs. The withdrawal signs produced in animals dependent on butorphanol (kappa opioid receptor agonist) were similar to those of morphine (mu opioid receptor agonist). Besides the behavioral modifications, opioid withdrawal affected G protein expression in the central nervous system. The G-protein ${\alpha}-subunit$ has been implicated in opioid tolerance and withdrawal. The effects of continuous infusion of morphine or butorphanol on the modulation of G protein ${\alpha}-subunit$ mRNA were investigated by using in situ hybridization study. In situ hybridization showed that the levels of $G\;{\alpha}s$ and $G\;{\alpha}i$ were changed during opioid withdrawal. Specifically, the level of $G\;{\alpha}s$ mRNA was decreased in the cortex and cerebellar granule layer during the morphine and butorphanol withdrawal. The level of $G\;{\alpha}i$ mRNA was decreased in the dentate gyrus and cerebellar granule layer during the morphine withdrawal. However, the level of $G\;{\alpha}i$ mRNA was significantly elevated during the butorphanol withdrawal. These results suggest that region-specific changes of G protein ${\alpha}-subunit$ mRNA were involved in the withdrawal from morphine and butorphanol.

• 한국산학기술학회논문지
• /
• 제6권2호
• /
• pp.183-188
• /
• 2005

포유동물에서는 두가지의 receptor가 보고되었다. 각 sample을 RNA extraction, RT-PCR, Realtime-PCR을 실시하여 melatonin 1A, 1B의 발현 양을 분석하였다. MT1A는 cerebellum에서는 3 hours에서 약 1/8배로 감소를 보이고 6 hours에서는 정상치 9 hours에서는 16배정도로 많은 양의 증가율 보였다. 나머지 hippocampus, thalamus, hypothalamus에서는 공통적으로 3 hours에서 많게는 10배에서, 적게는 대조군과 거의 비슷한 1.5배정도의 증가율을 보이고 있으며, 6 hours에서는 모두 감소하는 것을 알 수 있다. MT1B에서는 4 group 모두 3 hours, 6 hours에서 receptor의 양이 확연히 줄어들었다. 9 hours의 경우에는 4 group모두에서 적게는 8배, 많게는 거의 1000배 가까이의 발현 양의 차이가 나타났다.