• 한국어류학회지
• /
• 제12권3호
• /
• pp.208-214
• /
• 2000

성숙한 말쥐치 어미를 이용하여 인공수정에 의한 난발생과정과 성장에 따른 부화자어의 외부형태 발달을 관찰한 결과는 다음과 같다. 1. 수정란은 무색 투명한 구형의 분리침성점착란이다. 난경은 0.63~0.67 mm (평균 0.65 mm, n =50) 이고, 난황내에는 약 20~30개 정도의 크고 작은 유구들이 존재하였다. 2. 수온 $21.0\sim23.0^{\circ}C$에서 수정 후 약 53 시간만에 부화하였다. 3. 부화 직후 자어의 전장은 2.07~2.20 mm로 입과 항문이 열려있지 않았고, 근절은 5~6+17~18=22~24개이며, 체색은 대부분 투명하였다. 4. 부화 후 3~4일의 자어는 천장 2.62~2.77 mm로 입과 항문이 열려있었으며, 소형 rotifer를 섭이하기 시작하였다. 5. 부화 후 5일의 자어는 전장 2.74~3.04 mm로 머리 뒤쪽부분의 등쪽에 제1등지느러미 원 기인 등지느러미 가시가 새롭게 돌출하고 있었다. 6. 부화 후 12~13일의 자어는 전장 3.78~3.99 mm로 등지느러미 및 뒷지느러미의 원기 가 형성되고 있었다. 7. 부화 후 20일의 자어는 전장 6.04~6.17 mm였고, 이 시기에 등지느러미와 뒷지느러미의 지느러미 줄기수는 각각 19~20개, 18~19개, 꼬리지느러미에는 약 9~10개가 관찰되었다.

• 한국어류학회지
• /
• 제34권2호
• /
• pp.86-95
• /
• 2022

줄가자미의 난발생 및 자치어 형태발달을 관찰하여 종자생산 및 분류학적 연구의 기초자료로 이용하고자 한다. 2017~2018년까지 동해안에서 어미를 채집하여 원형수조(Ø 6 ×1 m) 수온 12.8±1.9℃에서 사육하였다. 수정란의 크기는 1.42~1.59 mm (평균±SD, 1.51±0.04 mm, n=50)였고, 난의 형태는 구형의 무색 투명한 분리부성란이었다. 난발생 과정은 수정 60분 후 난황과 난막이 분리되었고, 62시간 후 배체가 형성되었다. 수정 144시간 후 전체의 50% 이상이 부화가 완료되었다. 부화 직후의 자어는 전장 4.22~4.64 mm (4.53±0.16 mm, n=10)로 입과 항문은 열리지 않았고, 부화 후 10일째 전기자어는 전장 5.88~6.62 mm (6.31±0.33 mm, n=10)로 난황 흡수가 완료되고, 입이 열리기 시작하였다. 부화 후 55일째 중기자어는 전장 10.4~13.3 mm (12.7±1.3 mm, n=10)로 척추말단 끝부분은 휘어졌다. 부화 후 120일째는 전장 35.3~40.5 mm (39.5±2.4 mm, n=10)였고, 각 지느러미 기조 수는 등지느러미 79~94개, 뒷지느러미 63~75개로 정수에 달하면서 치어기로 이행하였다.

• 한국어류학회지
• /
• 제33권2호
• /
• pp.57-63
• /
• 2021

본 연구는 경북 낙동강에 서식하는 떡붕어의 초기생활사를 관찰하여 근연종과 차이점을 확인하기 위해 분류학적 기초자료를 확보하고자 하였다. 떡붕어 암컷의 산란량은 30,400~44,900개(37,650±7,250개) 정도였다. 난의 형태는 원형으로 크기는 1.24~1.37 mm (1.31±0.04 mm, n=30)였다. 수정란은 수온 21℃에서 부화까지 110시간이 소요되었다. 부화 직후의 난황자어는 전장 4.69~5.65 mm (5.15±0.31 mm, n=10)로 입과 항문은 열려있지 않았고 난황을 가지고 있었다. 부화 후 3일째 전기자어는 전장 6.27~6.70 mm (6.59±0.08 mm, n=10)로 난황 흡수가 완료되면서 먹이를 섭취하였다. 부화 후 10일째 후기자어는 전장 8.71~8.92 mm (8.81±0.07 mm, n=10)로 꼬리지느러미 말단 끝부분이 45°로 휘어졌다. 부화 후 42일째는 전장 15.1~16.8 mm (15.8±0.57 mm, n=10) 각 부위별 지느러미 기조 수(등지느러미 iii17개, 뒷지느러미 iii4개)가 정수로 도달하면서 치어기로 이행하였다.

• 한국발생생물학회지:발생과생식
• /
• 제18권4호
• /
• pp.241-249
• /
• 2014

Hyphessobrycon eques is a famous fish for ornamental fish market and aquarium. They are inhabit in regions of Amazon and Paraguay River basin. Serpae fishs were investigated 2-3 males are chased to female, and then males attempted to simulate the females abdomen. After fertilization, eggs were kept in incubators at $28^{\circ}C$. The fertilized eggs had adhesive and demesal characteristics and had a mean diameter of $0.92{\pm}0.01mm$. Larvae hatched at 16 hrs post fertilization. The hatched larvae averaged $2.90{\pm}0.16mm$ in total length ($L_T$). Complete yolk sac resorption and mouth opening occurred on the third day post hatching. At 45 days post hatching, the larvae were $12.5{\pm}1.60mm$ $L_T$ and had reached the juvenile stage.

• 한국응용곤충학회지
• /
• 제30권1호
• /
• pp.37-41
• /
• 1991

토양수분함량과 큰검정풍뎅이의 밀도 변화와의 관계를 파악하는데 필요한 기초 자료를 얻기 위해 토양수분함량이 큰 검정풍뎅이의 난과 유충의 생존에 미치는 영향을 조사하였다. 사양토와 식양토의 수분함량 15%와 25%에서는 난과 1, 2, 3령 유충 모두 79% 이상의 생존율을 보였으나 5%와 35%에서는 현저히 떨어졌다. 이 극한 수분함량에서는 토성에 따라 난과 유충의 생존율에 차이가 있는 것으로 보였다. 큰검정풍뎅이의 난은 생존할 수 있는 낮은 수분함량의 한계에 가까워질수록 발육기간이 길어졌다. 식양토에서 33-36%의 높은 수분함량에 난을 일정 기간 처리했을 때 배자 발육을 진행된 난일수록 잘 견뎠고 처리기간이 길어짐에 따라서 전란기간이 길어졌다. 또한 높은 토양수분함량에서는 3령 유충의 섭식 활동이 뚜렷이 억제되었다.

• 한국패류학회지
• /
• 제9권2호
• /
• pp.1-15
• /
• 1993

The reproductive ecology of the purple shell, Rapana venosa was investigated by the histological observations on depositions of the egg capsules, and hatching of larvae in the laboratory and the subtidal zone of the vicinity of piung-do, Chollabud-do, west coast of korea, for one year from June 1992 to May 1993. The results are summarized as follows:1. Rapana venosa is dioecious in sex. The ovary is composed of a number of ovarian lobules, and the testis comprises a number of ovarian lobules, and the testis comprises of gonads could be classified into 4 stages in males and 5 stages in females: 1) growing stage(in female subdivided into 2 stages of early and late growing stage). 2)mature stage. 3)spent stage or copulationstage. 4)rdcovering stage. The early growing stage in females of the purple shell was in September through February, late gorwing stage was in October to March, mature stage was in September to January, mature stage was in September to July, copulation stage was in Februaty to June and recovering stage in April to October.3. Spawning occurred 3-4 times at intervals of 1-3 days, and completed within 10 days from the beginning of spawning during the spawning season of the year.4. From the results of laboratory and field observations, egg masses are composed of a number of egg capsules, egg masses are occurred from May to late August, and in mid August depositions of egg mass in composed of 90-113 egg capsules, fecundity in an egg capsule was ranged 984 to 1,241 eggs(average 1,096 egg). Therefore, fecundity in total egg capsules spawned per individual during the spawning season is estimated as approximately 320,000 to 450,000 egges.5. The incubation period during deposition of an egg capsule to hatching larvad tood 17 days at 18.3-20.4%C(water temperature)and 1.021 (specific gravity fo sea water).

• Ocean Science Journal
• /
• 제40권1호
• /
• pp.11-16
• /
• 2005

Correction factors based on the catch ratios of egg and larval densities in the southern waters of Korea were estimated for anchovy Engraulis japonica. This was undertaken in order to adjust ichthyoplankton data from different sampling methods, gear types and time. Samples were collected during ichthyop1ankton surveys in Korean waters from 1983 to 1994. The ratios for egg densities obtained in vertical tows with a NORPAC net (ring $\Phi$, 45 cm) compared to those obtained in oblique tows with a KOB net (ring $\Phi$, 80 cm) were 0.86 (CV = 0.65), 1.22 (CV = 0.36), and 0.93 (CV = 0.42) for early, middle, and later developmental stages, respectively. The ratios for larval densities for vertical and oblique tows varied depending on size. For yolk-sac and small larvae (< 4 mm), the ratios were 3.08 (CV = 0.45) and 1.98 (CV = 1.34), while those of 4-6 mm, 6-8 mm, and 8-10 mm larvae were 0.44 (CV = 1.31), 0.45 (CV = 1.70), and 0.56 (CV = 2.50), respectively. Ratios of day/night densities for larvae of 4-10 mm lengths were lower (0.01-0.06) in offshore catches than values obtained in coastal areas (0.440.46) and similar values (0.16-0.04) for vertical and oblique tows. Our results indicated that vertical towing is more efficient for sampling early life stages (from eggs to larvae less than 4 mm long), while oblique towing is more efficient for larvae longer than 4 mm due to depth preferences for each developmental stage (e.g., changes in egg buoyancy and vertical migration oflarvae).

• Fisheries and Aquatic Sciences
• /
• 제24권11호
• /
• pp.375-382
• /
• 2021

In this study, the reproductive behavior and embryonic and larval development of the short ninespine stickleback Pungitius kaibarae was described and illustrated based on observations during spawning, hatching, and larval rearing trials. Adult P. kaibarae were collected downstream in Jinhae during the reproductive season (April-May). Males had nuptial coloration on their entire black bodies, with blue dorsal spines and yellow eyes, whereas females had a brown spotted pattern on their bodies. Males built nests on the stems of water weeds and attracted females. Fertilization occurred in the nest immediately after spawning, and males guarded the eggs until hatching. The fertilized eggs of P. kaibarae were spherical, demersal, adhesive, and transparent, and each egg measured 1.43 ± 0.07 mm in diameter. The morula, blastula, and gastrula stages, as well as hatching began at 5, 18.5, 21.5, and 96 post fertilization (HPF), respectively, at 20.0 ± 0.5℃. The newly hatched larvae had a total length (TL) of 5.67 ± 0.50 mm, with a yolk volume of 0.583 ± 0.059 mm³. Their mouths and anuses had not yet opened. At 2 days posthatching (days post hatching, DPH), the yolk was completely absorbed and the larvae began to feed exogenously. Pigmentation was observed in freshly hatched larvae 4 h after hatching, with the presence of eight areas with a dotted pattern on the dorsal surface of the larvae and dispersed spots on the head and yolk sac. At 30 DPH, the TL of the juveniles was 21.34 ± 1.70 mm. The nest area and number of eggs were 259.56 ± 101.39 mm² (75.18-506.04) and 155.33 ± 114.12 (0-437), respectively.

• 한국어류학회지
• /
• 제22권4호
• /
• pp.217-224
• /
• 2010

참홍어(Raja pulchra)는 서해지역에서 산업적으로 활용도가 높고 개체군 크기의 축소가 예상되므로 인공증식 기술 개발과 보존을 위해 일련의 연구를 수행하였다. 이 연구는 연중 대형 난각의 형태로 다회 산란하는 특성과 완전한 형태로 부화하는 치어의 특징을 기록하였다. 난소 내 난은 평균 179.8 (54~247)개로 직경이 5가지 무리로 구분되어 순차적인 산란에 기여하는 특성을 보였다. 난각은 다른 홍어류의 종과 구분되는 특징적 구조를 보였으며, 하나의 난각내에 포함되는 배의 수는 2~6개 범위이었다. 아울러 난각내에서 발생하는 자어와 부출 직전에 해당하는 치어의 형태적 특징 및 변화를 분석하여 기술하였다.

• 한국수산과학회지
• /
• 제44권5호
• /
• pp.516-523
• /
• 2011

To compare four populations of sea squirt Halocynthia roretzi adults, their egg quality and larval development were investigated in the hatchery. The populations were: south sea wild (SSW), south sea cultured (SSC), east sea wild (ESW) and Iwate Japan cultured (IJC). Egg quality and larval development were compared using 13 factors (fertilization rate and diameter, proximate composition, amino acid and fatty acid contents of eggs, hatching rate and various sizes of tadpole larvae) which were obtained from each population. Fertilized egg diameter, hatching rate and size of tadpole larvae were significantly different among the four populations (P<0.05). Eggs were produced with higher crude protein ($5.20{\pm}0.00%$ SSW, $4.71{\pm}0.01%$ ESW, $4.66{\pm}0.01%$ SSC and $3.96{\pm}0.01%$ IJC) and lipid ($1.22{\pm}0.01%$ ESW, $1.01{\pm}0.00%$ SSW, $0.77{\pm}0.01%$ SSC and $0.69{\pm}0.00%$ IJC,) contents from domestic wild populations than from Japanese or cultured populations. Also amino acid and fatty acid contents were different. The extent of similarity between domestic and Japanese populations (30.5% IJC:SSW, 34.3% IJC:SSC and 40.7% IJC:ESW) was relatively low but was very high between SSW and SSC (73.9%). These results may have been due to differences in the abundance of food types and environmental conditions in the four localities and consequent differences in the diets of the sea squirts.