• BMB Reports
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• 제34권1호
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• pp.59-65
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• 2001

In subtractive hybridization, target sequences in the tester are enriched by hybridizing with an excess amount of driver, followed by removing the tester hybridized with the driver. All of existing subtractive cloning methods are designed to remove the tester/driver hybrid. The removal of hybrid, however, is often unsatisfactory For various reasons. In this study we developed a subtractive enrichment protocol in which the tester/driver can be completely removed by selecting only the tester/tester after hybridization. In this protocol both the tester and driver DNAs are ligated with same linker DNAs and amplified by polymerase chain reaction (PCR). The tester DNA is then digested with two different enzymes and used in subsequent hybridization with an excess driver. After hybridization, the DNA is ligated with the adaptor that is only compatible with the tester/tester. Since only the tester/tester can have the new adaptor, no tester/driver can be amplified by PCR in this protocol. Unlike other methods, a 100% subtraction efficiency can be achieved even though the enzymatic treatments used in the enrichment procedure are incomplete. Furthermore, only the hybridized tester DNA can have the new adaptor and be amplified by PCR, resulting in 100% denaturation in effect. The efficacy of this novel method was verified with the model system in which a known amount of the target sequence is included.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2017년도 춘계학술대회
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• pp.645-648
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• 2017

기존의 Embedded Tester는 Log 처리를 위해 TCP와 공유 파일 시스템을 이용한 Server - Client간 1-N 구조로 처리 되었다. 이러한 방식은 구현 난이도에 따른 시간적 손실과 예외처리에 따른 Tester의 리소스 낭비가 발생한다. 이에 메시징 시스템을 이용하여 분산처리가 가능한 Log 처리 메시지 레이어를 구현하고 기존의 TCP, 공유 파일 시스템 전송방식과 비교하였다. 비교 결과 메시지 레이어를 이용한 전송이 TCP 보다 더 높은 전송 대역폭을 보였다. CPU 사용량에서 메시지 레이어가 TCP 보다 낮은 효율을 보였으나 큰 차이를 보이지 않았다. 이를 통해 메시지 레이어를 이용한 Log 처리가 더 높은 효율을 보임을 알 수 있었다.

• 청정기술
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• 제11권3호
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• pp.153-164
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• 2005

This study found a reliable evaluation for four kinds of pb-Free plating solutions and it's layers, through pure Sn, SnAg, SnBi and SnCu. These four kind of solutions are widely used to pb-Free plating. Hull-cell, Harring-blum, coverage, throwing power, current efficiency, stability, life-time, composition, hardness, roughness, abrasion, scratch, solderability, corrosion, contact angle, morphology, SIR(Surface insulation resistance) and Whisker test were experimented. Also, Using ICP, XRF, FE-SEM, EDS, temperature/humidity chamber, solderability tester, hardness tester, roughness tester, abrasion tester, salt spray tester, contact angle tester, SIR tester, and microscope. In this paper could be shown the systematic and various analysis for reliability about four kinds of pb-Free plating solutions, processes and it's deposit surface.

• 전기학회논문지P
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• 제59권1호
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• pp.83-88
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• 2010

A new live-line insulator tester was developed to detect faulty insulators in 154kV power transmission lines. This paper is the first part of the two-part paper and deals with its tester development. The developed tester has the following characteristics. First, it automates parts of the insulator inspection process by using a sensor and an actuator, and secondly, it sustains its weight by itself, not by a lineman. Finally, it measures the insulation resistance of an insulator together with its distribution voltage to provide more information for its analysis and diagnosis. These leads to the improvement of its operation efficiency, measurement reliability and usability. Its effectiveness was validated by live-line field tests in actual power lines.

• 소음진동
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• 제10권5호
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• pp.873-879
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• 2000

Experimental investigation was conducted to study the effects of pitch angle maximum camber on the performance and noise of an axial-flow fan used in outdoor-unit of air -conditioner. For this study the axial-flow fan whose pitch angle can be varied was made and the Specific sound Pressure Level and other coefficients were measured using the anechoic fan tester. It is found that pitch angle affects more severly than the maximum camber on the fan performance. On the while the maximum camber affects much on the specific sound power level. Present results show that it is important to choose the optimum pitch angle and maximum camber to design the high-performance and low-noise axial-flow fan and specific noise measured in the anechoic fan tester can be sued effectively for the design of low-noise fan.

• 인터넷정보학회논문지
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• 제11권5호
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• pp.71-80
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• 2010

이 시스템의 개발 목적은 상업화의 화두인 교육과 엔터테인먼트분야에서 새롭게 제작한 교육용 콘텐츠를 이용하여 현재 보급이 활성화중인 IPTV 상용화에 일조하는 것이다. 교육적 활용을 위하여 반응시험기(Stimulus $Tester^{TM}$)를 이용하였다. 반응시험기는 리모컨 또는 제 3의 도구로 학습 한 후 이에 대한 피드백으로서 반응 속도를 부여하는 시스템을 말한다. 이 시스템에서는 주어진 문제 해결 시간 동안에 학습자가 반응을 하면 정오반응 여부 및 그에 문제 해결에 소요된 시간에 대한 피드백이 제시된다. 이 과정에서 정오반응 여부와 더불어 반응시간 피드백을 통하여 학습자가 스스로의 학습능률 정도를 확인하게 되고 이후 학습 증진에 도움이 되도록 하였다. 이 피드백 과정은 개인 PC에서 연결된 서버를 통하여 대상자에게 다시 PC로 포인트를 배분하는 과정을 통하도록 하였다. 이 시스템의 보급을 통하여 IPTV의 활성화와 더불어 교육 콘텐츠 개발로 인한 점진적인 산업의 활성화를 기대할 수 있을 것으로 보인다. 이 시스템을 통한 학습능률을 증진여부를 검증하기위하여 일반 남녀대학생을 대상으로 인터넷기반과 페이퍼기반의 학습증진여부를 측정하였으며, 그 결과 우수한 경우 최대 지속적인 7일 반응으로 2.47분에서 1.27분으로 학습효과가 51% 증가하였다.

• 한국화재소방학회:학술대회논문집
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• 한국화재소방학회 2008년도 춘계학술논문발표회 논문집
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• pp.159-162
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• 2008

A standard fire extinguishing tester, with which we could conduct the fire extinguishing ability rating of a water-based fire extinguishing agent such as water and loaded stream, has been developed in this work. It could help us enhancing the efficiency and reliability of the fire extinguishing test of loaded streams, and at last increasing the productivity of fire protection related firms. Furthermore, our country could take the lead in making a new standard for the fire extinguishing test of loaded streams. As a result, it is expected that loaded streams could be improved by using it. In addition the standard fire extinguishing tester could be made moderately in our industry, and supplied at home and worldwide.

• 한국정보통신학회논문지
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• 제19권5호
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• pp.1228-1234
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• 2015

본 논문에서는 열전소자를 이용한 온도 환경 시험기에 적용할 수 있는 퍼지제어기를 개발하였다. 온도환경 시험기에 퍼지제어기를 구현하기 위해 마이컴기반의 온도제어시스템을 제작하였다. 안정적인 온도 유지 및 온도 변화 속도를 향상시키기 위하여 퍼지제어기를 내장한 온도환경 시험기를 개발하였다. 제안한 방법의 유용성을 입증하기 위하여, 일반적으로 널리 사용되고 있는 비례-적분-미분 제어기와 비교하였다. 실험결과 제안한 퍼지제어기가 기존의 비례-적분-미분 제어기보다 상승시간, 오버슈트 면에서 좋은 제어성능을 보임을 알 수 있었다.

• Tribology and Lubricants
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• 제25권6호
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• pp.387-398
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• 2009

The operation condition of recently designed pistons for high power and high speed diesel engine become more severe due to the increment of combustion pressure and temperature. So, in order to overcome high temperature, the application of the mono-metal cast aluminum alloy piston featuring an enclosed cast-in open cooling gallery has increased. In this research, it is developed a PCJ (piston cooling jet) rig tester, described the test procedure and validated the performance of sample piston cooling gallery design. Then the test rig will be used for developing the design technology of piston cooling gallery. The test rig is composed with oil reservoir and pumping system, oil jet system, piston fixing and moving system, collecting oil measuring system, and data measuring and recording system. It will be measured collecting efficiencies under conditions of a few piston positions, oil jet pressures and oil viscosities for a piston cooling gallery. Furthermore, the PCJ rig tester will be used for the optimum design of the oil cooling gallery which being applied to increase the cooling efficiency of pistons in diesel engines satisfying the EURO V emission regulation and the more.

• 한국기계가공학회지
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• 제19권3호
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• pp.99-104
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• 2020

Modern people spend a lot of time indoors. Oil mist has a very fatal effect on the cook's health. Range Hood's filter plays a very important role in removing oil mist from kitchen. In this study, we applied coanda effect to increase the collection efficiency of filter. Original, Bottom, Slide Three shapes were simulated, and the two shapes were measured and compared using a wind tunnel tester. Pressure loss is measured below 2.0mmAq for all three filters, making them easy to use indoors. The simulation results were 15% for the original filter, 22% for the bottom filter, and 30% for the slide filter. The wind tunnel tester obtained 26% of the original filter and 30% of the slide filter. Research is needed to increase the collection efficiency by 50%.