• 제목/요약/키워드: Effective Production

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고추의 소포자 배양 시 전처리 후 배지의 교환, 배지의 첨가 및 2층배양 시 하층고체 배지의 양이 배의 생산에 미치는 영향 (The effect of medium change after pretreating microspores, medium addition, and volume of under solid medium in double layer culture on the production of embryos in isolated microspore culture of hot pepper (Capsicum annuum L.))

  • 박은준;이종숙;안동주;김문자
    • Journal of Plant Biotechnology
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    • 제37권4호
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    • pp.494-504
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    • 2010
  • 본 연구에서는 고추의 소포자 배양 시 전처리 후 배지의 교환 여부, 배양 후 새 배지의 첨가 및 2층배지 사용시 하층고체배지의 양이 배의 생산에 미치는 영향을 조사하였다. 고온 전처리 후 배양배지를 첨가하여 배양하는 것보다 사용한 전처리 배지를 새 배양 배지로 교환하여 배양 하는 것이 배의 생산에 효과적 이었으며, 고온전처리 기간은 1일이나 2일에 비해 3일이 효과적 이었다. 배양 후 새 배지의 첨가는 약전처리 시에는 효과가 없었으나 소포자 전처리 시에는 배의 유기와 발달 모두 크게 향상되었다. 새 배지의 첨가 시기는 배양 4일 후가, 첨가 횟수는 1회가 배의 생산에 가장 효과적이었다. 한편 2층배지 사용 시 첨가하는 새 배지의 양은 1.5 ml이 효과적이었으며 이보다 많은 양을 첨가하는 경우 배의 발생과 발달 모두 저하되었다. 액체배지 사용 시에 비해 2층배지 사용 시 배의 발달이 좋았다. 또 2층배지 사용 시 하층고체배지의 양이 3 ml 일 때 보다는 5 ml이나 7 ml일 때 배의 발생은 감소하였으나 배의 질이 향상되었다. 이와 같은 결과들은 고추에서 다수의 정상자엽배를 생산 할 수 있는 소포자 배양시스템을 확립하는데 중요한 기초자료가 될 것이다.

Nighttime Cooling Is an Effective Method for Improving Milk Production in Lactating Goats Exposed to Hot and Humid Environment

  • Sunagawa, Katsunori;Nagamine, Itsuki;Kamata, Yasuhiro;Niino, Noriko;Taniyama, Yoshihiko;Kinjo, Kazuhide;Matayoshi, Ayano
    • Asian-Australasian Journal of Animal Sciences
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    • 제28권7호
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    • pp.966-975
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    • 2015
  • Heat production in ruminants follows a diurnal pattern over the course of a day peaking 3 hours following afternoon feeding and then gradually declining to its lowest point prior to morning feeding. In order to clarify the cooling period most effective in reducing decreases in feed intake and milk production, experiments were carried out based on the diurnal rhythm of heat production and heat dissipation. In experiment 1, the effects of hot environment on milk production were investigated. The animals were kept first in a thermoneutral environment ($20.0^{\circ}C$, 80.0%) for 12 days, they were then transitioned to a hot environment ($32^{\circ}C$, 80.0%) for 13 days before being returned to second thermoneutral environment for a further 12 days. In experiment 2, the effectiveness of daytime cooling or nighttime cooling for improving milk production in hot environment was compared. While ten lactating Japanese Saanen goats (aged 2 years, weighing 41.0 kg) during early lactation were used in experiment 1, ten lactating goats (aged 2 years, weighing 47.5 kg) during mid-lactation were used in experiment 2. The animals were fed 300 g of concentrated feed and excessive amounts of crushed alfalfa hay cubes twice daily. Water was given ad libitum. The animals were milked twice daily. When exposed to a hot environment, milk yield and composition decreased significantly (p<0.05). Milk yield in the hot environment did not change with daytime cooling, but tended to increase with nighttime cooling. Compared to the daytime cooling, milk components percentages in the nighttime cooling were not significantly different but the milk components yields in the nighttime cooling were significantly higher (p<0.05). The results indicate that nighttime cooling is more effective than daytime cooling in the reduction of milk production declines in lactating goats exposed to a hot environment.

수소생산을 위한 Enterobacter cloacae YJ-1의 배양조건 (Culture Conditions for Hydrogen Production of Enterobacter cloacae YJ-1)

  • 이기석;강창민;정선용
    • KSBB Journal
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    • 제19권6호
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    • pp.446-450
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    • 2004
  • 수소생산을 증진하기 위하여 탄소원의 농도가 수소생산에 미치는 영향을 조사하였다. 단일당으로 glucose를 이용하였을 때 최적 농도는 $2\%$로서 975.1 mL을 생산하였다. 혼합당을 이용하여 수소생산을 향상시킬 목적으로 그 혼합비를 조사한 결과 glucose와 sucrose의 비가 25 : 75이었을 때 가장 높은 수준을 보였다. 수소를 생산할 때 기질의 발효가 진행됨에 따라 배양액의 pH 저하가 생겨 세포성장에 영향을 주게 된다. 인산염의 첨가로 pH 저하에 따른 완충작용을 하여 생산능이 향상되었음을 확인할 수 있었다. 특히 회분식 배양시에는 배양액의 유출이 없기 때문에 수율을 향상하기 위하여 적절한 농도의 인산염의 첨가가 필수적이다. 미네랄의 영향을 시험하기 위하여 배지에 각종 미네랄원을 첨가하여 그 영향을 조사한 결과 $CuCl_2$$NiCl_2$을 제외하고 모두 높은 수소생산성을 나타내었다. 그 중에서도 Ferric citrate와 $Na_{2}MoO_4$에서 더 많은 수소가 생산됨이 확인되었다. 이는 수소생산에 있어 Ferric citrate와 $Na_{2}MoO_4$가 중요한 요소라고 생각된다. 반대로 $CuCl_2$$NiCl_2$는 필수적인 물질이 아님을 보여준다. 따라서 이들 Ferric Citrate와 $Na_{2}MoO_4$을 첨가하여 수소생산을 증가시키는 원인으로 앞으로 세밀히 관여한 효소의 생리적 역할에 많은 연구가 필요하다.

SWNTs-catalyzed solar hydrogen production

  • Kim, Young Kwang;Khan, Gulzar;Jeong, Hye Won;Park, Hyunwoong
    • Rapid Communication in Photoscience
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    • 제3권3호
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    • pp.56-58
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    • 2014
  • Single-walled carbon nanotubes (SWNTs) catalyzed hydrogen production from water containing various electron donors under visible light (${\lambda}$ > 420 nm). As-received SWNTs were effective for hydrogen production, yet the effect vanished when they underwent surface chemical treatments. Upon coupling with CdSe particles, however, the surface treated SWNTs were far superior to non-treated SWNTs by a factor of ~30 for hydrogen production.

Desktop program production

  • Enami, Kazumasa;Fukui, Kazuo;Yagi, Nobuyuki
    • 한국방송∙미디어공학회:학술대회논문집
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    • 한국방송공학회 1996년도 Proceedings International Workshop on New Video Media Technology
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    • pp.77-81
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    • 1996
  • In order to conform to the needs of effective program production in multimedia era, we are studying Desk Top Program Production system. With the DTPP, users can easily produce multimedia program including video, sound, and ancillary data, and freely handle video images synthesizing video components retrieved from video database. This paper describes the new program production system, DTPP and its key technologies such as cooperative program production via multimedia network, indexing and utilization of attribute information of images, and image segmentation and spatio-temporal editing.

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Simulation Based Production Using 3-D CAD in Shipbuilding

  • Okumoto, Yasuhisa;Hiyoku, Kentaro;Uesugi, Noritaka
    • International Journal of CAD/CAM
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    • 제6권1호
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    • pp.3-8
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    • 2006
  • The application of three-dimensional (3-D) CAD has been popularized for design and production and digital manufacturing has been spreading in many industrial fields. By simulation of the production process using 3-D digital models, which are the core of CIM (Computer Integrated Manufacturing) system, the efficiency and safety of production are improved at each stage of work, and optimization of manufacturing can be achieved. This paper firstly describes the concept of "simulation based production" in shipbuilding and also digital manufacturing; the 3-D CAD system is indispensable for effective simulation because ship structure is three dimensionally complex. By simulation, "computer optimized manufacturing" can be possible. The most effective fields of simulation in shipbuilding are in jobs where many parties have to cooperate, while existing two-dimensional drawings are hardly observed the whole structures due to interference between structures or equipment of complex shape. In this paper some examples of the successful application in IHIMU (IHI Marine United Inc.) are shown: assembly of a pipe unit, erection of a complex hull block, carriage of equipment, installation of a propeller, and access in an engine room.

Zoogloea ramigera의 회분식, 유가배양, 연속배양에 의한 생물고분자 생산 (Biopolyrner Production of Zoogloea ramigera in Batch, Fed-Batch and Continuous Culture Processes)

  • 안대희;정윤철
    • 한국미생물·생명공학회지
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    • 제20권2호
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    • pp.196-202
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    • 1992
  • Zooloea ramigera 115를 사용하여 생물응집제로서 사용되는 생물고분자생산 실험을 하였다. 생물고분자 생산을 높이기 위하여 회분식, 유가배양, 연속배양방법을 사용하였다. 탄소원으로는 포도당, 유당, 당밀, 유청을 사용하였다. 기질이 포도당의 경우에는 C/N배 98일 때 생물고분자 생산 효과가 좋았으며, 유당의 경우에는 C/N비 30, 당밀과 유청의 경우에는 C/N비 60일 때 생물고분자 생산이 가장 좋았다. 유가배양 방법이 회분식 배양방법 보다 최종 생물고분자 생산이 우수하였다. C/N비를 달리한 2단계 연속 배양방법으로 생산성을 향상시켰다. 당밀의 경우 0.048$hr^{-1}$의 희석속도에서, 유청의 경우 0.096$hr^{-1}$에서 생산성이 가장 좋았다.

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High-Level Production of Astaxanthin by Fed-Batch Culture of Mutant Strain Phaffia rhodozyma AJ-6-1

  • KIM, SU-JIN;GEUN-JOONG KIM;DON-HEE PARK;YEON-WOO RYU
    • Journal of Microbiology and Biotechnology
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    • 제13권2호
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    • pp.175-181
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    • 2003
  • The production of a carotenoid astaxanthin, a growth-associated principal pigment, is limited in a batch cultivation, because a high glucose concentration severely inhibits the cell growth and also influences the carotenoid production. Therefore, a fermentation strategy including effective chemicals for the high-level production of cells and astaxanthin by a mutant strain Phaffia rhodozyma AJ-6-1 was developed in a fed-batch culture. First, a production medium for maximizing the cell and astaxanthin yields was formulated and optimized. Using this optimized medium, the highest cell and astaxanthin concentrations obtained were about 38.25 g/1 and 34.77 mg/1, respectively. In addition, an attempt was made to increase the amount of astaxanthin using effective chemicals such as ethanol and acetic acid, which are known at an inducer and/or precursor of carotenoid synthesis. When either 10g/1 ethanol or 5 g/1 acetic acid was added to investigate the resulting astaxanthin content, a relatively high astaxanthin concentration or 45.62 mg/l and 43.87 mg/1, respectively, was obtained, and the cell concentrations also increased slightly under these conditions. Therefore, these results imply that a fed-batch culture of the mutant strain P. rhodozyma AJ-6-1 could be effectively employed in the commercial production of astaxanthin, although the factors affecting the productivity remain to be elucidated.

최적해저지형정보 추출을 위한 보간법 선정 (Selecting of Interpolation for Extraction of Optimal Submarine Topographic Information.)

  • 이종출;김희규;김남식;김성호
    • 한국측량학회:학술대회논문집
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    • 한국측량학회 2004년도 춘계학술발표회논문집
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    • pp.571-576
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    • 2004
  • In this study, we selected the most effective interpolation method using both the data of sounding the depth of submarine topography and volume-production computer program. After processing the data by volume-production program, and then we selected the most effective interpolation method which nearest actual value of dredged soil quantity. Thus, these interpolation could be used for describing the optimal submarine topography.

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The Effectiveness of New Power Generation and Energy Demand Reduction to Achieve Greenhouse Gas Reduction Goals in Building Area

  • Park, Seong-Cheol;Kim, Hwan-Yong;Song, Young-Hak
    • Architectural research
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    • 제18권2호
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    • pp.59-64
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    • 2016
  • Since the massive power outages that hit across the nation in September 2011, a growing imbalance between energy supply and demand has led to a severe backup power shortage. To overcome the energy crisis which is annually repeated, a policy change for deriving energy supply from renewable energy sources and a demand reduction strategy has become essential. Buildings account for 18% of total energy consumption and have great potential for energy efficiency improvements; it is an area considered to be a highly effective target for reducing energy demand by improving buildings' energy efficiency. In this regard, retrofitting buildings to promoting environmental conservation and energy reduction through the reuse of existing buildings can be very effective and essential for reducing maintenance costs and increasing economic output through energy savings. In this study, we compared the energy reduction efficiency of national power energy consumption by unit production volume based on thermal power generation, renewable energy power generation, and initial and operating costs for a building retrofit. The unit production was found to be 13,181GWh/trillion won for bituminous coal-fired power generation, and 5,395GWh/trillion won for LNG power generation, implying that LNG power generation seemed to be disadvantageous in terms of unit production compared to bituminous coal-fired power generation, which was attributable to a difference in unit production price. The unit production from green retrofitting increased to 38,121GWh/trillion won due to the reduced energy consumption and benefits of greenhouse gas reduction costs. Renewable energy producing no greenhouse gas emissions during power generation and showed the highest unit production of 75,638GWh/trillion won, about 5.74 times more effective than bituminous coal-fired power generation.