• Interdisciplinary Bio Central
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• 제4권1호
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• pp.1.1-1.7
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• 2012

Background: The F-box proteins represent one of the largest families of proteins in eukaryotes. Apart from being a component of the ubiquitin (Ub)/26 S proteasome pathways, their regulatory roles in other cellular and developmental pathways have also been reported. One interesting feature of the genes encoding the proteins of this particular family is their variable selection patterns across different lineages. This resulted in the presence of lineage specific F-box proteins across different species. Findings: In this study, 48 non-redundant F-box proteins in E. siliculosus have been identified by a homology based approach and classified into three classes based on their variable C-terminal domains. A greater number of the F-box proteins have domains similar to the ones identified in other species. On the other hand, when the proteins having unknown or no C-terminal domain (as predicted by InterProScan) were analyzed, it was found that some of them have the polyglutamine repeats. To gain evolutionary insights on the genes encoding the F-box proteins, their selection patterns were analyzed and a strong positive selection was observed which indicated the adaptation potential of the members of this family. Moreover, four lineage specific F-box genes were found in E. siliculosus with no identified homolog in any other species. Conclusions: This study describes a genome wide in silico analysis of the F-box proteins in E. siliculosus which sheds light on their evolutionary patterns. The results presented in this study provide a strong foundation to select candidate sequences for future functional analysis.

• 한국산학기술학회논문지
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• 제13권11호
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• pp.5615-5622
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• 2012

광양만의 3개의 다른 지역에서 바이러스 개체수와 물리화학적 요인의 계절적 분포도를 분석하였다. 또한 바이러스의 형태적 구조를 투과전자현미경을 이용하여 관찰하였다. 대부분의 해양바이러스는 박테리오파지와 유사한 구조를 갖고 있었으나, 일부는 진핵 미세조류 바이러스인 Ectocarpus siliculosus virus (EsV)와 유사하였다. 그룹 1 (폐쇄된 부영양 지역)에서의 바이러스 개체수는 그룹 2 (폐쇄된 빈영양 지역) 및 그룹 3 (개방된 빈영양 지역)에서의 바이러스 개체수보다 높았다. 그룹 1 바이러스의 계절적 개체수는 크게 변화하였다. 바이러스 개체수는 8월에 가장 높은 $7.33{\times}10^8ml^{-1}$에서 12월에 가장 낮은 $0.3{\times}10^8ml^{-1}$로 변화하였고, 봄과 가을에는 중간값을 유지하였다. 질소원 및 인산원의 농도는 바이러스 개체수에 상관관계를 보였다. 특히 용존산소의 계절적 변화는 바이러스 개체수와 반대 경향을 보였다. 이러한 결과는 광양만에서의 해양바이러스 개체수가 물리화학적 환경과 밀접하게 연관되어 있음을 보여준다.

• ALGAE
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• 제27권1호
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• pp.21-32
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• 2012

We report an oxidative burst triggered by prostaglandin $A_2(PGA_2)$ in the brown algal kelp Laminaria digitata, constituting the first such discovery in an alga and the second finding of an oxidative burst triggered by a prostaglandin in a living organism. The response is more powerful than the oxidative burst triggered by most other chemical elicitors in Laminaria. Also, it is dose-dependent and cannot be inhibited by diphenylene iodonium, suggesting that another source than NAD(P)H oxidase is operational in the production of reactive oxygen species. Despite the very strong oxidative response, rather few effects at other levels of signal transduction pathways could be identified. $PGA_2$ does not increase lipolysis (free fatty acids) in Laminaria, and only one oxylipin (15-hydroxyeicosatetraenoic acid; 15-HETE) was found to be upregulated in Laminaria. In a subsequent set of experiments in the genome model Ectocarpus siliculosus, none of 5 selected candidate genes, all established participants in various stress responses, showed any significant differences in their expression profiles.