• 한국미생물·생명공학회지
• /
• 제51권1호
• /
• pp.37-42
• /
• 2023

Neutral and non-essential amino acid, glutamine (Gln), plays an essential role in supplying nitrogen to all the amino acids and nucleotides in the mammalian body. Gln is also the most important carbon source that provides intermediates for gluconeogenesis and fatty acid synthesis and supplements the tricarboxylic acid cycle in fast-growing cancer cells. Among the known 14 Gln transporter genes, soluted carrier family 1 member 5 (SLC1A5) has been reported to be closely associated with cancer cell growth. Three variants (v1, v2, and v3) have been derived from SLC1A5. Here, we established a heterologous gene expression system for the active form of human SLC1A5 variant-2 (hSLC1A5v2) in Escherichia coli. v2 is the smallest variant that has not yet been studied. Four expression systems were investigated: pBAD, pCold, pET, and pQE. We also addressed the problem of codon usage bias. Although pCold and pET overexpressed hSLC1A5v2 in E. coli, they were functionally inactive. hSLC1A5v2 using the pBAD system was able to catalyze the successful transport of Gln, even if it was not highly expressed. Initial activity of hSLC1A5v2 for [¹⁴C] Gln uptake in E. coli reached up to 6.73 μmole·min^-1·gDW^-1 when the cell was induced with 80 mM L-arabinose. In this study, we demonstrated a heterologous expression system for the human membrane protein, SLC1A5, in E. coli. Our results can be used for the functional comparison of SLC1A5 variants (v1, v2, and v3) in future studies, to facilitae the developement of SLC1A5 inhibitors as effective anticancer drugs.

• 한국초등과학교육학회지:초등과학교육
• /
• 제32권3호
• /
• pp.250-259
• /
• 2013

This study was aimed at developing eco-STEAM educational programs based on smart learning, implementing the programs to verify their educational effectiveness, and exploring the possibilities for eco-education. The subjects of Science, Mathematics, Practical Arts, Arts, and Physical Education were analyzed to extract STEAM elements for the 5th and 6th grades at elementary school, and then 16 lessen plans were developed under 8 thematic strands. The programs were applied to classes of 5th and 6th graders, and then tested to see the effectiveness in terms of emotional experience, convergence, creative design and satisfaction. The average scores for post-test were statistically higher than those of pre-test(p<.001), showing positive effectiveness of the eco-STEAM programs developed. This study put out the following conclusions. First, the students got emotional experiences through inquiry and observation. Second, the programs helped students to learn about the environment in their contexts and provided a base for interdisciplinary approach. Third, the students in this study could have opportunities for improving their problem-solving abilities by using the creative design. Forth, the students' interests in the ecological topics were increased throughout regular curricula.

• 한국인쇄학회지
• /
• 제26권2호
• /
• pp.79-89
• /
• 2008

Eco printing offers the perfect balance between getting all our printing jobs done without endangering the environment. It is important to realize that the printing industry is no exception to this rampant destruction of the planet's natural resources. In fact, surprisingly enough, its effect on the environment is an extremely harmful one. Like most other production operations, printing houses also produce harmful waste products that pollute the air we breathe in. They also put a great drain on precious natural resources. Printing houses emit what are known as volatile organic compounds (VOCs), caused by the use of petroleum-based inks, laminates, varnishes and adhesives. Studies show that these compounds, when inhaled, greatly increase the risk of asthma attacks. Eco printing, however, has a larger scope than is apparent. Eco printing has a dual essence. In order to understand what it is really all about, the issue of conservation is no less important than the need to stop polluting the environment.

• Architectural research
• /
• 제17권4호
• /
• pp.147-152
• /
• 2015

Recently, there is an increasing criticism both inside and outside China toward the eco-migration projects. The author focuses on the eco-migration of Sanjiangyuan National Nature Reserve as a case study as it is one of the main eco-migration projects. At the end of 2010, government invested 4.47 billion CNY in eco-migration and relocated the immigration 55773 people in 10733 units. Benefits of eco-migration is obvious, however, it needs to be recognized that in the aspect of environment justice, it is unfair to sacrifice a group of people in the process of environmental protection because costs and benefits result from the environmental protection have to share among the people without discrimination. Local herdsmen received extra disadvantages in their level of material possessions, social and cultural values and the social inclusion after eco-migration project. As a part of the eco-social system established within several centuries, the local herdsmen are not only threateners to local ecology system, but also the guardians to the natural environment through religion and habits last for generations. The social and cultural values, traditional religion and Nomadic living habits, as important as the environment, are need to be cherished and protected.

• 한국프랜차이즈경영연구
• /
• 제13권2호
• /
• pp.33-51
• /
• 2022

Purpose: The online market is growing the most in history due to the expansion of non-face-to-face commerce. In addition, as consumers' interest in health, food safety, and environment increases, interest in and consumption of eco-friendly agricultural products is also increasing. Therefore, in the case of a specialty store that sells eco-friendly organic agricultural products, a marketing strategy that can increase customer loyalty by reflecting these consumer needs is necessary. In this study, the store attributes of eco-friendly food stores are classified into location, assortment, price, quality, and employee service, and the effect of each store attribute on utilitarian and hedonic value is investigated. Research design, data, and methodology: The subjects of this study were customers who visited an eco-friendly food store. Of the 511 survey responses, 311 were used for statistical verification, excluding 200 who had not visited within the last 3 months. For statistical analysis, Smart PLS 3.0 was used, and after checking the validity and reliability of the items, hypothesis testing was performed. Result: As a result of the study, it was found that assortment, quality, and employee service among store attributes had a positive (+) effect on utilitarian and hedonic value. Second, location had no significant effect on utilitarian and hedonic value. Third, price did not appear to have a positive (+) effect on the utilitarian value, and it was found to have a positive (+) effect on the hedonic value. Fourth, It was investigated whether the presence or absence of delivery service had an effect on store attributes between utilitarian and hedonic value, and it was found that there was a significant effect between employee service and hedonic value. Conclusions: Among eco-friendly food store environment management will be required in order to provide food that meets the tastes and needs of consumers by diversifying the taste, standard, and quality grade of food, and to maintain or improve the quality. In order to unlike other stores, eco-friendly food stores have high price resistance from the point of view of consumers, so it is necessary to diversify promotional media such as YouTube and SNS to raise awareness of eco-friendly organic food.

• 한국생활과학회지
• /
• 제16권3호
• /
• pp.505-522
• /
• 2007

This study examined the effects of eco-system factors on adolescents' suicidal ideation and whether self-esteem and depression mediate in the process to provide theoretical basis for prevention and treatment for problems of adolescents who have suicidal ideation. 2,088 high school students were asked to complete self-administering questionnaires on eco-system factors and adolescents' suicidal ideation. Data were analyzed with frequencies, t-test, ANOVA with Scheffe ad-hoc test, correlation analysis using SPSS 12.0 program and AMOS 5.0 program. Study results were ; First, adolescents' suicidal ideation showed significant differences according to their gender, socio-economic financial status, family structure, and the type of school they attended. Second, self-esteem and depression were proved to have significant mediating effect. Child abuse victimization, subjective school achievement and influence of media coverage of celebrity suicide were most important variables which influenced adolescents' suicidal ideation. These results reinforce importance of understanding adolescents' suicidal ideation with eco-system perspective. Moreover, prevention programs for adolescents' suicide require differentiated strategies and tactics according to the target group characteristics.

• 교육시설 논문지
• /
• 제18권2호
• /
• pp.3-14
• /
• 2011

This study aims to identify differences and lessons in eco-school planning techniques and sustainable design methods by analyzing comparatively green building certification system and the cases of sustainable schools in US, Korea and Japan. As a result of the comparative analysis, green building certification system for school facilities, both domestic and international, is categorized into external environment, energy, materials and resources, and indoor environment. From the case study, it is common that roof garden and biotopes are installed for external environment, while energy saving, passive energy utilization methods for natural lighting and ventilation such as arrangement planning, courtyard, top-light, shading devices, solar panel and insulation by roof garden are most frequently used. Also, storm water uses, water saving equipment and sustainable materials are often introduced for resource savings. Concerns for indoor environment is frequently addressed by introducing natural light and ventilation in the buildings, which makes ultimately a comfortable space.

• 한국경영과학회지
• /
• 제40권1호
• /
• pp.91-116
• /
• 2015

This study aims to provide public sectors with eco-efficiency information. To implement the purposes of the study, environmental and economic variables of Eco-Efficiency were identified through decision tree model, then the relative Eco-Efficiencies of 243 public sectors were evaluated through input-oriented DEA (Data Envelopment Analysis) model. Specifically, the amount of public purchasing per a staff and the amount of energy use per a staff were considered as input factors. Sales per a staff was considered as output factor. The result shows that most of the public sectors (94.2%) were evaluated as "inefficient" taking into consideration of average value, 0.501 from market-based public corporations, 0.288 from local public corporations, 0.28 from quasi-market-based public corporations, 0.269 from fund-management-based quasi-governmental institutions, 0.09 from non-classified public institutions, and 0.078 from commissioned-service-based quasi-governmental institutions. Furthermore, it is possible to establish a plan for internal Eco-Efficiency improvement based on information of the reference set. In order to improve the Eco-Efficiency in the public sectors in the long term, environmental impacts of the overall public sectors' operations (e.g., energy saving, water saving, waste reduction, and purchasing of green products) needs to be properly proposed in consideration of BSC (Balanced Scorecard) indicators of public sectors.

• Journal of Nutrition and Health
• /
• 제48권1호
• /
• pp.105-112
• /
• 2015

본 연구는 친환경 식재료를 이용하여 학교급식을 운영하고 있는 제주지역 초등학교의 학부모 530명을 대상으로 친환경급식에 대한 인식도 및 만족도를 조사 분석함으로써 친환경급식에 대한 학부모의 인식을 제고시키고 친환경급식의 신뢰성 및 급식효과를 높일 수 있는 방안을 모색하기 위한 기초자료를 제공하고자 하였다. 조사대상의 친환경 식재료에 대한 인식도는 5점 만점에 대해 평균 3.52점으로 나타났고 친환경농산물과 일반농산물의 차이 (3.76), 친환경농산물의 종류 (3.51), 식품 구입할 때 농산물 품질마크나 원산지 등 확인 여부 (3.45), 친환경농산물 인증기준 및 표시 등의 인지도 (3.31)의 순으로 나타났으며 친환경농산물과 일반농산물의 차이 인지도에서 동지역 학부모의 인지도 (3.94)가 읍 면지역 학부모의 인지도 (3.79)보다 유의적으로 높게 나타났다 (p < .05). 조사대상의 친환경 식재료 사용에 대한 신뢰도는 '믿는 편이다'가 71.2%로 가장 높게 나타났고 친환경급식 실시 여부 인지도에 있어서는 조사대상 학부모의 75.0%가 '알고 있다'고 응답하여 대부분의 학부모가 인지하고 있음을 알 수 있었으며 친환경급식비 일부를 지방자치단체와 교육청에서 지원받고 있는 것을 알고 있는지에 대한 인지도는 조사대상의 53.5%만이 알고 있는 것으로 나타났다. 조사대상의 친환경급식 실시에 대한 동의 여부에 있어서는 조사대상의 98.3%가 동의하는 것으로 나타나 거의 모든 학부모가 친환경 학교급식을 실시하는 것에 동의하고 있음을 알 수 있었고 친환경급식의 찬성 이유에 대해서는 '건강 안전성의 확보'가 87.0%로 가장 높게 나타났다. 조사대상의 친환경급식 만족도는 5점 만점에 대해 평균 3.88점으로 나타나 비교적 만족도가 높음을 알 수 있었고 식재료의 안전성 (3.98), 알맞은 영양 공급 (3.94), 몸에 좋은 식재료 (3.91), 음식의 품질 (3.89), 음식의 맛 (3.70)의 순으로 나타나 식재료의 안전성과 영양적인 측면에 대한 만족도가 높음을 알 수 있었다. 조사대상 학부모들의 친환경급식 이후 학교급식 만족도변화에 있어서는 만족도 증가 (55.0%), 만족도 변화없음 (42.3%), 만족도 감소 (2.7%)의 순으로 나타나 절반 정도의 학부모들이 친환경급식 이후 학교급식에 대한 만족도가 증가되었음을 알 수 있었다. 친환경 급식 이후 조사대상자의 만족도가 높아진 이유에 대해서는 안전한 음식 제공 (70.5%), 급식의 질 향상 (26.3%), 음식의 맛 향상 (2.6%), 기타 (0.5%)의 순으로 나타났다. 조사대상의 친환경 식재료에 대한 인식도, 친환경급식에 대한 인식도 및 친환경급식 만족도간의 상관관계를 분석한 결과 친환경 식재료에 대한 인식도는 친환경급식에 대한 인식도와 약한 정 (+)의 상관관계 (r = 0.234) (p < .01), 친환경 식재료에 대한 인식도와 친환경급식에 대한 인식도는 친환경급식에 대한 만족도와 약한 정 (+)의 상관관계 (r = 0.287, r = 0.254)를 나타내 (p < .01), 친환경 식재료 및 친환경급식에 대한 인식도는 친환경급식에 대한 만족도에 유의한 영향을 미치고 있음을 알 수 있었다.

• 한국환경복원기술학회지
• /
• 제4권4호
• /
• pp.36-45
• /
• 2001

Natural disturbances and human development can cause habitat fragmentation. Plant and wildlife can become isolated, and habitat fragmentation and shrinkage have been recognized as a key issue facing the conservation of biological diversity. However, eco-corridors can alleviate the problem by providing linkages between isolated patches. The purpose of this study is to plan the eco-corridor for connecting urban forest via the urban stream with low cost and to restore the ecosystem. The results were as follows (1) Falco subbuteo, and Dryocopus martius inhabit in the study site. They are protected species designated by Korean Ministry of Environment. Thus the study site should be preserved as urban wildlife habitat species biodiversity (2) If the biodiversity of the study site is maintained properly, the eco-corridor can be constructed with low cost.