• Journal of Microbiology and Biotechnology
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• 제16권3호
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• pp.450-456
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• 2006

Pseudomonas fluorescens MC07 is a growth-promoting rhizobacterium that suppresses mycelial growth in fungi such as Rhizoctonia solani, Pythium ultimum, Fusarium oxysporum, and Phytophthora capsici. To determine the role of the bacterium's antifungal activity in disease suppression, we screened 2,500 colonies generated by Tn5lacZ insertions, and isolated a mutant 157 that had lost antifungal activity. The EcoRI fragment carrying Tn5lacZ was cloned into pBluescript II SK(+) and used as a probe to isolate wild-type clones from a genomic library of the parent strain, MC07. Two overlapping cosmid clones, pEH4 and pEH5, that had hybridized with the mutant clone were isolated. pEH4 conferred antifungal activity to the heterologous host P.fluorescens strain 1855.344, whereas pEH5 did not. Through transposon mutagenesis of pEH4 and complementation analyses, we delineated the 14.7-kb DNA region that is responsible for the biosynthesis of an antifungal compound. DNA sequence analysis of the region identified 11 possible open reading frames (ORF), ORF1 through ORF11. A BLAST search of each putative protein implied that the proteins may be involved in an antifungal activity similar to polyketides.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권5호
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• pp.393-399
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• 2004

Useful genes can be Screened from various environments by construction of metagenomic DNA libraries. In this study, water samples were collected from several lakes in mid Korea, and analyzed by T-RFLP to examine diversities of the microbial communities. The crude DNAs r were extracted by the SDS-based freezing-thawing method, and then further purified using an $UltraClean^{TM}$ kit (MoBio, USA). The metagenomic libraries were constructed with the DNAs partially digested with EcoR I, BamH I, and Sac II in Escherichia coli DH 10B using the pBACe3.6 vector. About 44.0 Mb of metagenomic libraries were obtained with average inserts 13-15 kb in size. The bphC genes responsible for degradation of aromatic hydrocarbons via mets-cleavage were identified from the metagenomic libraries by colony hybridization using the bphC specific sequence as a probe. The 2,3-dihydroxybiphenyl (2, 3-DHBP) dioxygenase gene (bphC ), capable of degradation of 2,3-DHBP, was cloned and its nucleotide Sequences analyzed. The genes consisted of 966 and 897 base pairs with an ATG initiation codon and a TGA termination codon. The activity of the 2,3-DHBP dioxygenase was highly expressed to 2,3-DHBP and Showed a broad substrate range to 2,3-DHBP, catechol, 3-methylcatechol and 4-methylcatechol. These results in-dicated that the bphC gene identified from the metagenomes derived from lake water might be useful in the development of a potent strain for degradation of aromatic pollutants.

• KIEAE Journal
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• 제13권5호
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• pp.89-96
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• 2013

Green roofs can reduce surface water runoff, provide a habitat for wildlife moderate the urban heat island effect, improve building insulation and energy efficiency, improve the air quality, create aesthetic and amenity value, and preserve the roof's waterproofing. Green roofs are mainly divided into three types : intensive, simple-intensive, and extensive. Especially, extensive roof environment is a harsh one for plant growth; limited water availability, wide temperature fluctuations, high exposure to wind and solar radiation create highly stressed environment. This study, aimed at extensive green roof, was carried out on the rooftop of the library at Seoul Women's Univ. from October to November, 2012 and from March to August, 2013. To suggest the most effective vegetation model for biodiversity and heat island mitigation, surface temperatures were monitored by each vegetation model. We found that herbaceous plants of Aster sphathulifolius, Aceriphyllum rossii and Belamcanda chinensis, shrub of Syringa patula 'Miss Kim', Thymus quinquecostatus var. japonica, Sedum species can mixing each other. Among them, the vegetation models including Sedum takesimense, Aster sphathulifolius, Thymus quinquecostatus var. japonica was more effective on the surface temperature mitigation, because the species have the tolerance and high ratio of covering, and also in water. Especially, in the treatment of bark mulching, they helped to increase the temperature of vegetation models. In the case of summer, temperature mitigation of vegetation models were no significant difference among vegetation types. Compared to surface temperature of June, July and August were apparent impact of temperature mitigation, it shows that temperature mitigation are strongly influenced by substrate water content.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.525-531
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• 2005

From a genomic library of Lactobacillus reuteri ATCC 55739, one clone, NE347, carrying a pyrH gene encoding UMP kinase, was identified. pNE347 carried a 1.88 kb EcoRI fragment and the pyrH was located in the middle of the insert. pyrH ORF was 723 bp in size and capable of encoding UMP kinase composed of 240 amino acid residues. tsf encoding an elongation factor-Ts and frr encoding a ribosomal recycling factor were present upstream and downstream of pyrH, respectively. When introduced into E. coli KUR1244, a pyrH-negative strain, pNE347 restored the ability to grow at $42^{\circ}C$, indicating that pyrH from L. reuteri synthesized functional UMP kinase in E. coli. Northern blot experiment showed that pyrH and frr were cotranscribed as a 1.4 kb single transcript. pyrH was overexpressed in E. coli by using a pET26b(+) vector, and a major 25 kDa protein band appeared on SDS-polyacrylamide gel.

• 농업과학연구
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• 제38권4호
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• pp.641-649
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• 2011

This study was to analyze the soil environmental characteristics and vegetation status of green roof in Daejeon Metropolitan City. The investigated floras of vascular plants are 17 families, 26 genera, 28 species in Seo-Gu Daejeon District Office Building (SG), 25 families, 49 genera, 56 species in Galma Public Library (GP), and 34 families, 57 genera, 60 species in Daejeon City Hall (DC) respectively. Although the larger area shows the more numbers of species in introduced plants and naturalized plant, the naturalized plant ratios were similar with each other. They were 10.71%, 10.71%, and 11.67% at SG, GP, and DC respectively. As a result of analysis on soil physical property, soil depths including vegetation soil and drainage soil of 3 green roofs were 30cm. The depths of vegetation soil at SG, GP, and DC were 0~8cm, 0~10cm, 0~10cm respectively. As a results of soil chemical properties of our study, soil pH of vegetation soil and drainage soil were a range of 6.42 and 7.43, and a range of 6.55 and 7.43 on the average respectively. Available-P contents of vegetation soil and drainage soil were a range of 153.33 and 366.33mg/kg, and a range of 136.67 and 242.67 mg/kg which is very high, respectively. Carbon contents in soil at vegetation soil and drainage soil were a range of 3.16 and 6.38%, and a range of 1.63 and 2.47% respectively. Carbon storage per square meter within 30 cm were 2.76 kg, 2.99 kg, and 3.66 kg at SG, GP, and DC respectively.