• Journal of Plant Biotechnology
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• v.46 no.1
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• pp.22-31
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• 2019

The objective of this study was to investigate the suitable parts for callus induction and optimal concentrations of growth regulators contained in the medium affecting shooting and rooting Echeveria laui and Echeveria elegans for in vitro mass production. To determine the suitable plant parts for callus induction, the leaves were divided into upper, medium and bottom parts and cultured on MS medium at different concentrations with $0{\sim}2mgL^{-1}\;NAA$ and $0{\sim}4 mgL^{-1}BA$. The upper and middle parts of leaves both showed 100% callus formation rate with $NAA\;1\;mgL^{-1}$ and $BA\;1\;mgL^{-1}$ treatment in E. laui. The middle parts of leaves showed 83.3% callus formation rate at $NAA\;2\;mgL^{-1}$ and BA 4 mgL-1 treatment in E. elegans. The shoot induction rate from callus was highest at $NAA\;0.1\;mgL^{-1}$ and $BA\;3\;mgL^{-1}$ treatment in E. laui and $NAA\;0.3\;mgL^{-1}$ in E. elegans. In addition, the number of shoots formation was 10.4 shoots high in $NAA\;1\;mgL^{-1}$ and $BA\;1\;mgL^{-1}$ treatment in E. laui and 12.0 shoots in most effective $NAA\;1\;mgL^{-1}$ and $BA\;0.1\;mgL^{-1}$ treatment in E. elegans. In the case of acclimatization of regenerated plant, growth characteristics did not show any significant difference (35 ~ 55%) shading with respect to the different ratio of substrate mixture, and it was determined that would be appropriate considered plant height and appearance preference of E. laui and E. elegans. It was established that the optimization of culture condition was responsible for the mass propagation in vitro cultures of E. laui and E. elegans.

• Journal of Bio-Environment Control
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• v.27 no.4
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• pp.363-370
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• 2018

The succulent plants of Echeveria genus are in increasing demand worldwide, but it is difficult to supply good quality young plants throughout the year because propagation efficiencies are depend on cultivar and environmental factors. This study was carried out to investigate the propagation efficiencies of leaf cutting in Echeveria cultivars at different LED light qualities in a closed-type plant factory system. Leaf cuttings cut from stock plants of six difficult-to-propagated cultivars 'Afterglow (AG)', 'Berkeley Light (BL)', 'Mason (MS)', 'Subsessilis Light (SL)', 'Cream Tea (CT)', and 'Ben Badis (BB)' were put into cutting media in the plant factory system maintained at a temperature of $24{\pm}2^{\circ}C$ and relative humidity of $60{\pm}10%$, and watered with over-head irrigation twice a week. Cuttings were irradiated with sole or mixed red (R, 660 nm), blue (B, 450 nm), green (G, 530 nm), and far-red (FR, 730 nm) LEDs as follows: R10, R8+B2, R5+B5, R7+B2+FR1, and R7+B2+G1. PPFD just above the cuttings was $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and photoperiod was 16/8 (light/dark) hours. As a result, propagation efficiencies were dependent on cultivar. Rooting and shooting were relatively easy in 'SL' but shoot formation in 'AG' was very difficult. Light qualities from LEDs also affected plant regeneration. Light conditions with a higher ratio of B, R5+B5, R7+B2+FR1, and R7+B2+G1, promoted shoot formation and growth but inhibited rooting and root growth. R10 and R8+B2 with a higher ratio of R promoted rooting and root growth and inhibited shoot formation and growth of cuttings. In addition, the treatment with FR increased leaf size and biomass of the all plants. Therefore, further studies are needed to investigate the optimum compositions of LED light quality for the improvement of leaf cutting efficiency in difficultto-propagated Echeveria cultivars.

• Journal of Practical Agriculture & Fisheries Research
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• v.8 no.1
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• pp.30-42
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• 2006

This experiment was conducted to investigate current situation and plant grouping by light and watering of mingled several cacti and succulents in a pot. The main 11 cacti are consist of Myrtillocactus geometrizans (Mart.) Cons., Gymnocalycium mihanovichii var. friedrichii Werd., Marginatocereus marginatus(DC.) Backbg., Eriocactus leninghausii (Hge. Jr.) Backbg, Notocactus scopa (Spreng.) Berg., Lobivia nealeana Backbg., Mammillaria elongata var. intertexta (DC.) SD., Pseudoespostoa (Vp1.) Backbg., Mammillaria albilanata Backbg., Mammillaria klissingiana Bod. and Espostoa lanata (HBK.) Br. & R. The main 10 succulents are Kalanchoe thyrsifolia Harv., Portulacaria afra var. foliis-variegatis Jacobs., Crassula perforata Thunbg., Echeveria pulvinata Rose, Aeonium arboreum cv. atropurpureum., Echeveria lauii Moran & J. Meyran, Pachypodium lamerei Drake, Sedum rubrotinctum cv. Aurora Boom., Aloe mitriformis Mill., and Euphorbia coerulescens Haw. The cacti were native to Mexico, Brazil, Peru, Paraguay, Ecuador, etc. The sucullents were native to Mexico, Africa, Gran Canaria, Madagascar, etc. Most of all the cacti and sucullents in the experiment are growing well under full sun or light shade, however Portulacaria afra var. foliis-variegatis Jacobs, and Sedum rubrotinctum cv. Aurora Boom. did not influenced by light. And all the plants require a little or moderate watering. Thus, planting by similar required water and light, when growing in a pot of several cacti and succulents, might be decreased the rate of death causing by their different plant physiology.