• 한국작물학회지
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• 제20권
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• pp.95-99
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• 1975

해바라기에 있어서 일장반응의 품종간차이를 알고자 1974년 7월부터 10월까지 조중만생종 3개 품종을 폿트에 파종하여 옥내인 온실과 옥외인 자연상태에서 단일 및 장일의 일장처리를 하여 각 품종의 개화반응을 조사한 결과를 요약하면 다음과 같다. 1. 조생종인 Peredovic의 개화기는 8시간 및 12시간구에서 비슷하고 16시간구보다 10일 단축되였으나 각 일장처리구 모두 실내가 옥외보다 11∼15일 빨랐다. 2. 중생종인 Hungary의 개화기는 옥내에서 각 일장처리구가 거의 비슷하였으나 옥외에서는 12시간구가 8시간구 및 16시간구보다 7∼11일 빨랐다. 3. 만생종인 군포종의 개화기는 제일 늦었으나 8시간 및 12시간구에서는 비슷하고 16시간구보다 14일 단축되였으나 옥내가 옥외보다 10∼21일 빨랐다. 4. 조생종인 Peredovic은 옥내외의 적산온도차가 적어 일장보다 온도가 그리고 중만생종인 Hungary와 군포종은 옥내외 적산온도차가 커 온실보다 일장에 더 예민한 것 같다. 5. 해바라기의 개화반응은 숙기의 조만에 거의 관계없이 8∼12시간의 단일하에서 개화일수가 단축되고 16시간구에서 연장되었으며 그 정도는 품종간 차이를 보였다.

• 한국환경농학회지
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• 제19권5호
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• pp.394-400
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• 2000

${\gamma}-Radiation$ at very low doses frequently has a stimulating or hormetic effect on the growth of organism. Effects of low dose ${\gamma}-ray$ irradiation on the root growth of soybean cultivars were investigated and hormetic effects by environmental conditions were compared with the occurrence of increased economic yield, seeds of cultivars were irradiated with the dose of $0.5{\sim}20Gy$ and cultivated in growth chamber controling temperature, humidity, light, greenhouse and field respectively. To understand hormetic effect on root growth of cultivars and the difference of hormetic effect by cultivation environment, harvested root of soybean cultivars were scanned with image file, and root surface area, root length, root average diameter etc. were examined by WinRhizo program. Also, dry weight of cultivars was examined. Root growth and dry weight of soybean cultivars showed apparently hormetic effect at cultivation of growth chamber condition. In field experiment executed for whole life cycle, yields of pea were not different significantly in each ${\gamma}-ray$ irradiated cultivars but weight of one hundred peas increased in whole ${\gamma}-ray$ irradiated cultivars. Increment of yield was assumed to be induced through shortening of maturing stage caused by ${\gamma}-ray$ hormesis in early growth stage.

• 대한한의학회지
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• 제18권2호
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• pp.273-282
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• 1997

To observe the effect of Heulbuchuketang on endometriosis, surgically induced endometriosis in rats was treated with Heulbuchuketang for l00days. The result are as follows: 1. Fragments of right uterine horn were implanted at the serosal wall of small intestine and its adjacent aterial suppling branch. After 4 weeks later endometriosis was observed at the area of implantation of small intestine. 2. After l00-day-treatment, the size of the endometriosis of treated group was much smaller compared to those of the untreated group. Especially the result of the later 60-day-treatment was much better than that of the early 40-day-treatment. 3. In case of inflammation or pustula induced from endometriosis, the size of abnormal endometriosis tissue showed no change after the total treatment and fibrotic change was often founded there, which was not responding to Heulbuchuketang. 4. In the ovary of untreated group, primordial follicle, primary follicle, maturing primary follicle were easily founded and there was no difference in shape and size between the ovary of Heulbuchuketang treated group. From the upper 1 to 4 laboratory result it can be suggested that Heulbuchuketang can be specially effective to mild endometriosis with conservating the ability of fertility.

• 한국가축번식학회지
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• 제15권3호
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• pp.207-220
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• 1991

This study was carried out to investigate the ultrastructural changes of ooplasm and follicular membrane of oocytes, obtained from 150 of 3-year-old female rainbow trout(Oncorhynchus mykiss). All data were collected from March in 1989 to February in 1990, and from August to October in 1991. The size of the nucleoli and number of the yolk granules increased as the oocyte growed. Yolk granules were deposited in the oocyte as crystalline granules. Due to the presence of large early and late maturing oocytes, their ovaires were enlarged, transparent and granular. The lattice was broken down at hydration, leaving the egg transparent. As thepercentages of fish in LPO and EMO stage increased from September to October, Mean GSI values increased. Follicle cells such as granulosa cell and thecal cell change a squamous into cuboid shape in LPO and EMO stage. Seasonal changes in the microscopic appearance of the ovaries were well correlated with those in bothgonadosomatic index and macroscopic apearance. Under the natural conditions,t he ovarian follicle influences the histological development and periodical secretion of the hormones, sufficient for a oogenesis and gonadal steroid production. The electrophoretic pattern of major band in mature stage was much thicker(70∼110k dalton) than that in previtellogenic phase.

• Reproductive and Developmental Biology
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• 제29권4호
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• pp.241-245
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• 2005

Porcine oviduct epithelial cells (POEC) are widely used in co-culture experiments to improve early embryonic development, in vitro fertilization in embryo transfer programs for domestic animals and in vitro maturation of immature germ cells. POEC were mechanically isolated and cultured in tissue culture medium 199. Cells grew continuously, and confluent monolayers were formed after 7 days. After forming confluent monolayer of epithelial cells, supernatant was collected as the condition medium for maturing round spermatids in vitro. Round spermatids were also separated mechanically and cultured in the POEC condition medium. In this study we observed that $20\%$ of round spermatid cultured were matured into elongating spermatid after 24 h, and about $10\%$ of round spermatid cultured showed complete elongation (elongated spermatid) within $24\~48$ h of in vitro culture. No further development was observed within $50\~72$ h and transformed cells lost their viability after 72 h. These preliminary findings suggest that the condition medium from POEC may be possible to overcome the round spermatid block by improving the milieu of culture system.

• 한국가축번식학회지
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• 제16권3호
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• pp.247-260
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• 1992

This study was carried out to investigate the histomorphological changes of ovaries obtained from 100 of 1-year-old female Korean loach(misgurnus anguillicaudatus). The light microscopic and ultrastructural changes ofooplasm and follicular membranes of oocytes, were observed by lightand transmission electron microscope during the reproductive cycle. All data were collected from November in 1991 to May in 1992. The results obtained in this study were as follows: The size of the nucleoli and number of the yolk granules increased as the oocytes grown. Yolk granules were loosely deposited in the oocytes as crystalline granules. Due to the presence of large early and late maturing oocytes, their ovaries were enlarged, transparent, granular and yellowish in color. The lattice was broken down at hydration, leaving the egg transparent. As the percentages of fish in LMO and RO stage increased from March to April, mean gonadosomatic index(GSI) values(18.49%) increased. Zona radiata change a squamous into cuboid shape in EMO stage. Processes from the granulosa cells and from the oocyte, microvilli grow and make contact with other in the pore canals of the zona radiata during vitellogenesis, but are withdrawn as the zona radiata becomes more compact and devoid of pore canals during oocyte maturation. Seasonal changes in the microscopic appearance of the ovaries were well correlated with those in both GSI and macroscopic appearance.

• Plant Resources
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• 제3권3호
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• pp.194-199
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• 2000

The result of this experiment which are conducted, to improve the cultivation technology of Alisma plantago, to increase its quantity and to contribute for stable production with Yongiun local group by examining the optimal planting density and transplanting period of double cropping of Alisma plantago in the southern region. The characters of plant height, leaf width and length tend to be reduced as the seeding period is later by the order of the 10th, 20th and 30th of July. The period required for flowering is reduced as the transplanting period is later and dense planting is applied. Plant height, the number of leaves and yield of dry root have much quantity at the dense planting density of 20$\times$ 15cm as they are transplanted later in the 30th of August or the 10th of September, but they are rather less in sparse planting density of 20$\times$25cm or 20$\times$35cm.

• 한국토양비료학회지
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• 제14권3호
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• pp.146-156
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• 1981

볏짚 시용량(施用量) 차이(差異)가 수도분벽(水稻分蘗)에 미치는 영향(影響)을 알아보기 위(爲)하여 볏짚 시용량(施用量), 질소량(窒素量), 시용위치(施用位置) 및 배수(排水) 등(等)을 달리하여 시험(試驗)한 결과(結果)는 다음과 같다. 1. 볏짚 시용량(施用量) 증가(增加)는 초기분벽(初期分蘗)을 억제(抑制)하며, 최고분벽기출수기(最高分蘗期出穗期)를 지연(遲延)시키고 등숙율을 저하(低下)시켰다. 2. 볏짚 시용(施用)에 의(依)한 초기분벽기(初期分蘗期) 및 출수기(出穗期) 지연(遲延)을 단축(短縮)시켜 주지 못하였다. 3. 초기분벽(初期分蘗) 억제(抑制) 방지(防止)로서는 배수(排水)와 심층(深層) 시용(施用)이 효과적(效果的)이였다. 4. 볏짚 시용(施用)으로 토양(土壤)의 유기물(有機物) 및 칼륨의 증가(增加)가 현저(顯著)하였다.

• 한국작물학회지
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• 제7권1호
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• pp.117-121
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• 1969

춘파과맥 ${\ulcorner}$완주봄보리${\lrcorner}$의 종자를 5~100ppm의 지벨레린의 용액에서 백체출현기까지 최아하여 발아 및 포장시험을 실시한바 그 성적을 요약하면 다음과 같다. 1. 지벨레린최아에 으하여 발아율의 감퇴없이 2 (상온구)~3일(저온구)의 발아촉진을 보였다. 2. 지벨레린최아를 하면 초기생육촉진이 일면으로는 주장적현상이 현저하였는데 그경향은 고농도 일수록 심하다. 3. 지벨레린최아로 근생장의 억제작용은 전연 인정되지 않았다. 4. 포장조건에는 발아촉진의 효과가 별로없었고 고농도처리에서는 발아율의 감퇴를 초래하여 수수감소 및 수량감소를 초래하였다. 5. 고농도일수록 초기의 주장화경향이 심하고 회복도 늦으나 생육중기에 가서는 거의 모두 정상으로 회복되며, 따라서 출수 성숙 및 간장이 처리에 의한 변이를 보이지 않았다. 6. 5~10ppm의 농도에서는 발아저해가 적고 초기의 주장화경향이 적으며 수수감퇴도 적은 반면에 수장이나 천립중은 증대경향이어서 결과적으로 중수경향을 보였으나 재 검토가 필요하다. 7. 지벨레린 최아만으로는 발아촉진은 기대되나 조숙화는 기대되지 않는다.

• Asian-Australasian Journal of Animal Sciences
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• 제12권3호
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• pp.336-340
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• 1999

Age at maturity was studied in 661 Nili Ravi buffaloes maintained at six dairy farms in Pakistan. The mean age at maturity in the overall data from the six farms was $976.49{\pm}9.2$ days. Significantly lower mean age at maturity $(957.93{\pm}10.68\;days)$ was observed at Military Dairy Farm, Khyber Okara, Military Daiiy Farm, Punjnad and Livestock Research Station, National Agricultural Research Centre, Islamabad (Group I) compared to $(1015.26{\pm}17.39\;days)$ other three Military Dairy Farms, Peshawar, Nowshera and Rawalpindi (Group II). The advantages associated to early age at maturity were as following. Male and female calves were heavier $(38.35{\pm}0.17\;and\;31.84{\pm}15kg,\;respectively)$ in Group I as compared to $(29.27{\pm}0.26\;and\;26.27{\pm}0.26kg)$ in Group II. Milk yield per lactation was significantly higher in Group I $(1912{\pm}12\;lit.)$ as compared to $(1833.36{\pm}16.56\;lit.)$ in Group II. Lactation length was significantly longer $(284.41{\pm}1.23\;days)$ in Group I as compared to $(277.77{\pm}2.02\;days)$ in Group II. Dry period and service period were significantly shorter $(241.59{\pm}4.18\;and\;217.05{\pm}4.95\;days,\;respectively)$ in Group I as compared to $(306.39{\pm}78\;and\;280.95{\pm}9.32\;days)$ in Group II. The mean age at first calving and sex ratio were low ($1282.75{\pm}10.14$ days and 100 ♀ ♀:130.7 ♂ ♂) in Group I as compared to ($1308.7{\pm}16.44$ days and 100 ♀ ♀:152.15 ♂ ♂) in Group II but the differences were non significant.