• Title/Summary/Keyword: Early region

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Transcriptomic analysis of 'Campbell Early' and 'Muscat Bailey A' grapevine shoots exposed to freezing cold stress (영하의 저온에 노출된 'Campbell Early'와 'Muscat Bailey A' 포도나무 신초의 전사체 비교)

  • Kim, Seon Ae;Yun, Hae Keun
    • Journal of Plant Biotechnology
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    • v.43 no.2
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    • pp.204-212
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    • 2016
  • To understand the responses of grapevines in response to cold stress causing the limited growth and development, differentially expressed genes (DEGs) were screened through transcriptome analysis of shoots from 2 grapevine cultivars ('Campbell Early' and 'Muscat Baily A') kept at -$2^{\circ}C$ for 4 days. In gene ontology analysis of DEGs from 'Campbell Early', there were 17,424 clones related with biological process, 28,954 with cellular component, and 6,972 with molecular function genes in response to freezing temperature. The major induced genes included dehydrin xero 1, K-box region and MADS-box transcription factor family protein, and MYB domain protein 36, and inhibited genes included light-harvesting chlorophyll B-binding protein 3, FASCICLIN-like arabinoogalactan 9, and pectin methylesterase 61 in 'Campbell Early' grapevines. In gene ontology analysis of DEGs from 'Muscat Baily A', there were 1,157 clones related with biological process, 1,350 with cellular component, and 431 with molecular function gene. The major induced genes of 'Muscat Baily A' included NB-ARC domain-containing disease resistance protein, fatty acid hydrozylase superfamily, and isopentenyltransferase 3, and inhibited genes included binding, IAP-like protein 1, and pentatricopeptide repeat superfamily protein. All major DEGs were shown to be expressed differentially by freezing temperature in real time-PCR analysis. Protein domain analysis using InterPro Scan revealed that ubiquitin-protein ligase was redundant in both tested grapevines. Transcriptome profile of shoots exposed to cold can provide new insights into the molecular basis of tolerance to low-temperature in grapevines, and can be used as resources for development new grapevines tolerant to coldness.

Deflection Analysis of Flexural Composite Members Considering Early-Age Concrete Properties (콘크리트의 초기재령특성을 고려한 합성형 휨 부재의 재령종속적 처짐해석)

  • 성원진;김정현;윤성욱;이용학
    • Proceedings of the Korea Concrete Institute Conference
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    • 2003.05a
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    • pp.427-432
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    • 2003
  • An analytical method to predict the flexural behavior of composite girder is presented in which the early-age properties of concrete are specified including maturing of elastic modulus, creep and shrinkage. The time dependent constitutive relation accounting for the early-age concrete properties is derived in an incremental format by expanding the total form of stress-strain relation by the first order Taylor series with respect to the reference time. The sectional analysis calculates the axial and curvature strains based on the force and moment equilibriums. The deflection curve of the box girder approximated by the quadratic polynomial function is calculated by applying to the proper boundary conditions in the consecutive segments. Numerical applications are made for the 3-span double composite steel box girders which is a composite bridge girder filled with concrete at the bottom of the steel box in the negative moment region. The one dimensional finite element analysis results are compared with those of the three dimensional finite element analysis and the analytical method based on the sectional analysis. Close agreement is observed among the three methods.

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Overexpression of a delayed early gene hlg1 of temperate mycobacteriophage L1 is lethal to both M. smegmatis and E. coli

  • Chattoraj, Partho;Ganguly, Tridib;Nandy, Ranjan Kumar;Sau, Subrata
    • BMB Reports
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    • v.41 no.5
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    • pp.363-368
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    • 2008
  • Two genes of temperate mycobacteriophage L5, namely, gp63 and gp64, were hypothesized to be toxic to M. smegmatis. An identical L5 gp64 ortholog (designated hlg1) was cloned from homoimmune mycobacteriophage L1 and characterized at length here. As expected, hlg1 affected the growth of M. smegmatis when overexpressed from a resident plasmid. HLG1 (the protein encoded by hlg1) in fact caused growth retardation of M. smegmatis and the region encompassing its 57-114 C-terminal amino acid residues was found indispensable for its growthretardation activity. Both nucleic acid and protein biosynthesis were severely impaired in M. smegmatis expressing HLG1. Interestingly, HLG1 also affected E. coli almost similarly. This putative delayed early lipoprotein did not participate in the lytic growth of L1.

The Technologies of Plowing and Hoeing in 17th Century (17세기의 이경법(犂耕法) 및 서지법(鋤地法))

  • Hwang, Youn Gi;Lee, Ho Chol
    • Current Research on Agriculture and Life Sciences
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    • v.5
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    • pp.193-204
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    • 1987
  • The main purpose of this study is to examine agricultural technologies through plowing and hoeing. One of the purposes on which this study is to analysis process of development and divergence of plough and hoe. The following facts are significant findings ; 1. In early Yi - dynasty, tilling implements used by ard plough and mould board plough did use in three south province, most region mainly used ard plough. Therefore, farming in early Yi - dynasty is extensive. 2. The mould board plough diffused in many fann house, spring farming and autumnfarming formed complet system in early 17th Century. But works of hoeing was continuing to save labor by long hand hoe. 3. Finally in 17th century, Small - scale independent farming ceaselessly tried to make his basis with transtortation intensive farming method. But Small - scale independent farming had not stafely base because extensive farming method continued in this peorid.

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Situation Analysis of Existing Facilities for Screening, Treatment and Prevention of Cervical Cancer in Hospitals/Primary health Centers of Delhi-NCR Region, India

  • Chawla, P. Cheena;Chawla, Anil Kumar;Shrivastava, Richa;Shrivastava, Anju;Chaudhary, Seema
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.13
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    • pp.5475-5482
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    • 2014
  • Cervical cancer, the second most common malignancy all over the world, is associated with HPV infection. In a developing country like India, lack of early detection and treatment facilities is the main cause for its high burden. Therefore, through our study we e tried to present the current scenario of existing facilities for the detection and treatment of cervical cancer in hospitals and primary health centers (PHCs) of Delhi-NCR region. Data were collected from 312 healthcare facilities including public and private hospitals and PHCs of all nine districts from Delhi-NCR region. Healthcare providers including gynecologists, medical officers, women health care providers and paramedical staff were interviewed, using a questionnaire; the facilities for screening, diagnosing, and treating cervical cancer in each institution were recorded, using a previously designed checklist. Our study has shown that the basic facilities for the detection and treatment of cervical cancer are abhorrently lacking in Public hospitals and PHCs as compared to the Private hospitals in Delhi-NCR region. This study demonstrates that there is an urgent need for more investment in the diagnosis and treatment of cervical cancer facilities in public and rural healthcare facilities of Delhi-NCR region.

Microcalcification Detection Based on Region Growing Method with Contrast and Edge Sharpness in Digital X-ray Mammographic Images (명암 대비와 에지 선예도를 이용하는 영역 성장법에 의한 디지털 X선 맘모그램 영상에서의 미세 석회화 검출)

  • Won, C.H.;Kang, S.W.;Cho, J.H.
    • Journal of Sensor Science and Technology
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    • v.13 no.1
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    • pp.56-65
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    • 2004
  • In this paper, we proposed the detection algorithm of microcalcification based on region growing method with contrast and edge sharpness in digital X-ray mammographic images. We extracted the local maximum pixel and watershed regions by using watershed algorithm. Then, we used the mean slope between local maximum and neighborhood pixels to extract microcalcification candidate pixels among local maximum pixels. During increasing threshold value to grow microcalcification region, at the maximum threshold value of the contrast and edge sharpness, the microcalcification area is decided. The regions of which area of grown candidate microcalfication region is larger than that of watershed region are excluded from microcalcifications. We showed the diagnosis algorithm can be used to aid diagnostic-radiologist in the early detection breast cancer.

The Discussion on Treatment Based on Pattern Identification in Guidelines for Traditional Chinese Medical Treatment of COVID-19 in China (COVID-19 중국 진료방안의 변증론치에 대한 고찰)

  • Sanghyun, Kim
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.36 no.5
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    • pp.163-168
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    • 2022
  • After the outbreak of COVID-19 in China, the national health commission of the people's republic of China distributed guidelines for the diagnosis and treatment of COVID-19. Based on that, each region of China made guidelines for traditional Chinese medical treatment of COVID-19 applicable to clinical field. Under the hypothesis that each region's guideline contains regional characteristics, a comparison was made on pattern identification among each region's guidelines and central guidelines. At the beginning of the analysis of the cases, opinions on pattern identification vary from region to region, and the diversity is mainly reflected in the early stage of the disease. When the guideline is organized to a certain level due to the accumulation of clinical cases, there is a strong tendency to enumerate various types of pattern identification. It means that as a specific infectious disease progresses, it can appear in various cases due to variables. In some guidelines, disease stages were analyzed by only a limited pathological mechanism, but no regional characteristics were found here. Rather, it may mean that unique characteristics for disease can be derived.

ATOMIZATION PROCESS OF DIESEL FUEL SPRAY IN THE INITIAL STAGE OF INJECTION

  • KO K. N.;LEE C. S.;HUH J. C.
    • International Journal of Automotive Technology
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    • v.6 no.1
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    • pp.9-14
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    • 2005
  • An experimental investigation has been carried out to reveal the atomization process of the diesel fuel spray. The spray injected through a single hole nozzle was taken by a camera on the opposite side of a stroboscope for macroscopic observation or a nanolite for microscopic observation. The effect of nozzle aspect ratio was analyzed with disintegration phenomena of the diesel spray. Based on the enlarged spray photograph, atomization process was observed in detail and further the spray cone angle was measured under various ambient pressures. The result shows that atomization of diesel spray in early stage of injection is mainly progressed in the vicinity of spray periphery region except the region close to the nozzle exit and spray head region. The spray cone angle is nearly constant under the pressurized condition, while it decreases with elapsing time under the atmospheric condition.

NO OPEN CLUSTER IN THE RUPRECHT 93 REGION

  • Cheon, So-Ra;Sung, Hwan-Kyung;Bessell, M.S.
    • Journal of The Korean Astronomical Society
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    • v.43 no.4
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    • pp.115-121
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    • 2010
  • UBVI CCD photometry is obtained for the Ruprecht 93 (Ru 93)region. We are unable to confirm the existence of an intermediate-age open cluster in Ru 93 from the spatial distribution of blue stars. On the other hand, we find two young star groups in the observed field: the nearer one (Ru 93 group) comprises the field young stars in the Sgr-Car arm at $d{\approx}2.1$ kpc, while the farther one (WR 37 group) is the young stars around WR 37 at $d{\approx}4.8$ kpc. We derive an abnormal extinction law ($R_V$ = 3.5) in the Ruprecht 93 region.

Association of a Single Nucleotide Polymorphism in the 5'-Flanking Region of Porcine HSP70.2 with Backfat Thickness in Duroc Breed

  • Chen, Ming-Yu;Huang, San-Yuan;Lin, En-Chung;Hseu, Tzong-Hsiung;Lee, Wen-Chuan
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.1
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    • pp.100-103
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    • 2003
  • Higher environmental temperature affects the economic performance of pigs. Heat shock protein 70 has been shown to play an important role in thermoresistance. The purpose of this study was to assess the effect of a single nucleotide polymorphism in the 5'-flanking region of porcine HSP70.2 on growth performance in Taiwanese Duroc. The genotype of this nt 393 polymorphic site could be verified by digestion with Bsa WI restriction enzyme of a PCR product. Pigs with TT and TC genotypes have thinner backfats than those with CC type (p<0.05). The result suggested that the polymorphic Bsa WI site in the 5'flanking region of porcine HSP70.2 may be used as a marker for the early selection of ultrasonic backfat thickness in Duroc pigs.