• Journal of Microbiology
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• v.34 no.1
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• pp.30-36
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• 1996

We have exmained the re-establishment of HIMRE mediated silencing function on the transcriptional activity of yeast heast shock gene HSP82. To test whether the onset of SIR repression can occur in growing cells in the rpesence of a potent inhibitor of DNA replication, HMRa/HSP82 strains with SIR4- and SIR4S$^{+}$ genetic backgrounds were arrested in S phase by incubation of a culture in 200 mM hydroxyurea for 120 min. It was clear that following a 20 minute heat shock, silencing of the HMRa/HSP82 allele in cells pretreated with hydroxyurea does occur in a SIR4-dependen fashion, even though the kinetics of repression appears to be substantially delayed. We also have tested whether re- establishement of silencing at the HMR/hsp82 locus can occur in G1-arrested cells. Cell cycle arrest at G1 phase was achieved by treatment of early log a cell cultures with .alpha.-factor mating pheromone, which induces G1 arrest. The result suggests that passage through S phase (and therefore DNA replication) is nor required for re-establishing silencer-mediated repression at the HMNRa/HSP82 locus. Finally, to test whether de nono protein synthesis is required for re-establishment of silencer-mediated repression, cells were pretreated with cycloheximide (500 /.mu.g/ml) 120 min. It was apparent that inhibiting protein synthesis delays, but does not prevent, re-establishment of silencer-mediated repression. Altogether, these results indicate that re-establishment of silencer-mediated repression is not dependent on the DNA replication and has no requirement for protein synthesis.s.

• Advances in pediatric surgery
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• v.8 no.1
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• pp.54-61
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• 2002

Hirschsprung's disease (HD) is usually diagnosed in the newborn period and early infancy. The common presentation of HD in newborns consists of a history of delayed passage of meconium within the first 48 hours of life. The differential diagnosis in newborns is one of the clinical challenges of this disorder. A number of medical conditions which cause functional obstruction of the intestines are easily excluded. Neonates with meconium ileus, meconium plug syndrome, distal ileal atresia and low imperforate anus often present in a manner similar to those with HD in the first few days of life. Abdominal radiographs may help to diagnose complete obstruction such as intestinal atresia. Microcolon on contrast enema can be shown in cases with total colonic aganglionosis, ileal atresia or meconium ileus. Suction rectal biopsy or frozen section biopsy at operation is essential for differential diagnosis in such cases. HD is also considered in any child who has a history of constipation regardless of age. Older children with functional constipation may have symptoms that resemble those of HD and contrast enema is usually diagnostic. However, children with other motility disorders generally referred to as chronic idiopathic intestinal pseudoobstruction present with very similar symptoms and radiographic findings. These disorders are classified according to their histologic characteristics.; visceral myopathy, visceral neuropathy, intestinal neuronal dysplasia (IND), hypoganglionosis, immature ganglia, internal sphincter achalasia. Therefore, the workup for motility disorders should include rectal biopsy not only to confirm the presence of ganglion cells but also evaluate the other pathologic conditions.

• Proceedings of the KIEE Conference
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• 1994.07b
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• pp.1559-1561
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• 1994

At present, the principle of puffer action in high current interruption is adopted in almost of the EHV(Extra High Voltage) and UHV(Ultra High Voltage) GCB(Gas Circuit Breakers). The thermal interruption capability of these GCBs critically depends on the pressure rise in the puffer cylinder at current zero. The pressure rise in the puffer cylinder depends on the puffer cylinder volume, flow passage and leakage area in the interrupter, stroke curve etc. Recently commercial CFD(Computational Fluid Dynamics ) packages have been widely adopted to calculate the pressure distribution in the interrupter. However, there are still several problems with it, e.g. very expensive price, moving boundary problem, computation time, difficulty in using the package etc. Thus, the calculation of the puffer cylinder pressure in simple and relatively correct method is essential in early stage of GCB design. In these paper, the model ing technique and computed results for EHV class GCB (HICO, 145kV 40kA and 362kV 40kA GCB) are presented and compared with available measured results.

• Journal of Animal Reproduction and Biotechnology
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• v.36 no.4
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• pp.314-322
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• 2021

Paired box protein, PAX7, is a key molecule for the specification, maintenance and skeletal muscle regeneration of muscle satellite cells. In this study, we identified and characterized the cDNA and amino acid sequences of PAX7 from black sea bream (Acanthopagrus schlegelii) via molecular cloning and sequence analysis. A. schlegelii PAX7 cDNA was comprised of 1,524 bp encoding 507 amino acids and multiple sequence alignment analysis of the translated amino acids showed that it contained three domains including paired DNA-binding domain, homeobox domain and OAR domain which were well conserved across various animal species investigated. Pairwise Sequence Alignment indicated that A. schlegelii PAX7 had the same amino acid sequences with that of yellowfin seabream (A. latus) and 99.8% identity and similarity with that of gilt-head bream (Sparus aurata). Molecular phylogenetic analysis confirmed that A. schlegelii PAX7 formed a monophyletic group with those of teleost and most closely related with those of the fish that belong to Sparidae family including A. latus and S. aurata. In the investigation of its tissue specific mRNA expression, the expression was specifically identified in skeletal muscle tissue and a weak expression was also shown in gonad tissue. The cultured cells derived from skeletal muscle tissues expressed PAX7 mRNA at early passage but the expression was not observed after several times of subculture.

• International Journal of Advanced Culture Technology
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• v.11 no.1
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• pp.263-269
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• 2023

Zhang Yu was a famous Taoist priest and poet in Yuan Dynasty, compared with the identity of Taoist and poet, his handwriting had greater influence in history. According to the relevant historical data, people at that time were mainly attracted by his Taoist identity and poetic talent. However, if we considered him as a poet,or a calligrapher to inspect,it would be often easier than facing a Taoist priest. Zhang Yu's poems and relationships can be used as some clues for our investigation of his calligraphy activities,not only would it not affect us to judge the value of his calligraphy, but also we will comprehensively and objectively analyze his calligraphy works. His early calligraphy closely followed Zhao Mengfu, in his later years, when he met Yang Weizhen, the style of writing changed greatly.During this period, what kind of changes and fundamental influence had on Zhang Yu's life and book learning thoughts experienced. Our contributions to this paper are as follows.To solve this issue,this passage will try to find clues from several representative figures and representative calligraphy works of Zhang Yu, and draw on the research results of relevant scholars to discuss the identity of Zhang Yu as a Taoist priest and the origin and style characteristics of his handwriting and probed into the status and influence of his calligraphy in history.

• The Bulletin of The Korean Astronomical Society
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• v.46 no.2
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• pp.81.1-81.1
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• 2021

Stellar kinematics is a useful tool to understand the formation and evolution of young stellar systems. Here, we present a kinematic study of the HII region, NGC 821, using the Gaia Early Data Release 3. NGC 281 contains the open cluster IC 1590. This cluster has a core and a low-stellar density halo. We detect a pattern of cluster expansion from the Gaia proper motion vectors. Most stars radially escaping from the cluster are distributed in the halo. We measure the 1-dimensional velocity dispersion of stars in the core. The velocity dispersion (1 km/s) is comparable to the expected virial velocity dispersion of this cluster, and therefore the core is at a virial state. The core has an initial mass function shallower than that of the halo, which is indicative of mass segregation. However, there is no significant correlation between stellar masses and tangential velocities. This result suggests that the mass segregation has a primordial origin. On the other hand, it has been believed that the formation of young stars in NGC 281 West was triggered by feedback from massive stars in IC 1590. We investigate the ages of stars in the two regions, but the age difference between the two regions is not comparable to the timescale of the passage of an ionization front. Also, the proper motion vectors of the NGC 281 West stars relative to IC 1590 do not show any systematic receding motion from the cluster. Our results suggest that stars in NGC 281 West might have been formed spontaneously. In conclusion, the formation of NGC 281 can be understood in the context of hierarchical star formation model.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.9
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• pp.5931-5941
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• 2015

It should be given the opportunity to acquire information about the health of infants for a new type of way of life with the passage of time, of which the internet is a lot of people are required to use the media in the modern world. So, we want to find out the status of kindergarten teachers' internet utilizing and how to utilize the internet health information on field. The purpose of this study is to explore the direction of improvement. We produce this research based on a precedent study about perception and actual condition of health information utilizing. The objects of this study are 180 kindergarten teachers working in Dae-jeon and most of them have experience of searching health information through internet. The collected data calculated frequency and percentage with SPSS 18.0 program. As a result, first, kindergarten teachers think the information of infants' health is relatively important, still they obtain it from the Internet. But they feel a difficulty in finding the proper information because it takes so much time. Second, they utilize the Internet whenever necessary to find health information and they could find it. However, health information obtained from the Internet, have not been unexpectedly useful. Therefore, training about the content of infant's health and utilizing method of internet health information is needed.

• Clinical and Experimental Pediatrics
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• v.49 no.5
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• pp.482-488
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• 2006

Purpose : To evaluate the factors which contribute to the time of the first stool and the first urine passage. Methods : We retrospectively reviewed a chart of 1,221 infants ${\geq}34$ weeks of gestational age admitted to the normal newborn nursery of Il Sin Christian Hospital, Busan, from November 2004 to April 2005. We compared the time to first stool and urine according to maternal factors(maternal age, parity, mode of delivery, meconium-stained amniotic fluid, and maternal diabetes) and infant factors (gender, Apgar score, gestational age, type of feeding during first 24 hours, age at the first feeding, number of feeds during the first 12 hours and age at discharge). Results : In total, 95.3 percent of our infants had passed their first stool by 24 hours and 99.8 percent of them had a stool by 36 hours. A total of 95.8 percent of our infants had passed urine by 24 hours of age and 98.3 percent of them by 36 hours. Comparing preterm and term infants, the time to first urine is $6.5{\pm}5.8$ hours and $12.1{\pm}6.6$ hours, respectively(P=0.000). The time to first stool is $20.7{\pm}13.5$ hours and $10.0{\pm}6.3$ hours, respectively(P=0.000) Early-fed infants were significantly earlier in time to first urine(P=0.023) and first stool(P=0.012). There was no statistically significant relationship between the number of feeds in 0-12 hours, mode of delivery, Apgar score, parity, gender, type of feeding, maternal diabetes and the time of the first urine and first stool. Conclusion : Gestational age, birth weight and age at first feeding were significantly related to the time of the first urine and first stool passage. When there is delayed passage of the first urine and first stool, we should consider close observation of other associated symptoms and other factors previously mentioned, to avoid extensive evaluation and intervention.

• Food Science of Animal Resources
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• v.22 no.2
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• pp.137-144
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• 2002

Pork loins that retailed in market were used as experimental samples. Some pork samples in raw state were packaged with PVDC in either aerobic or vacuum condition. The other pork samples were cooked until core temperature reached at 70$\^{C}$ and then packaged immediately in the same way with the raw samples. After these samples were irradiated by electron beam 6 kGy, the samples were stored in a refrigerator (2∼4$\^{C}$). Identification and quantification of cholesterol oxides were performed at 0 and 7 days. The results were following. During the early stage of storage, cholesterol oxides were not produced from the raw meat samples, but with the passage of storage time,7 $\alpha$-hydroxycholesterol, 7$\beta$-hydroxycholesterol, 7-ketocholesterol, 20 $\alpha$-hydroxycholesterol, $\beta$-epoxide and $\alpha$-epoxide, which were not produced during the early stage of storage, were produced. The production of cholesterol and lipid oxidation products were significantly higher (P<0.05) in the meats with aerobic packaging than those with vacuum packaging, Cooked meat after irradiation showed 7 $\alpha$-hydroxycholesterol, 7 $\beta$-hydroxycholesterol, $\alpha$-epoxide and cholestanetriol on the 7th day of storage, although those chemicals were not produced during the early stage of storage. Production of cholesterol oxides was significantly increased (P<0.05) with the passage of storage time for all treatments, and showed significantly lower value (P<0.05) with the vacuum packaging than these for aerobic packaging. Species of cholesterol oxides from irradiated meat after cooking were similar to those from cooked meat after irradiation. Collectively, it was found that the production of cholesterol oxides was more easily affected by packaging condition than irradiation.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.5
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• pp.711-717
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• 2007

In this study, two cell types from porcine females, namely fetal fibroblasts (pFFs) and adult ear fibroblasts (pAEFs) and two passages (3-4 and 7-8) were investigated by evaluating the development rate, blastocyst cell number and the incidence of apoptosis. No significant differences were observed in the cleavage rates of cloned and IVF embryos. The blastocyst rates between the embryos cloned with pFFs ($15.1{\pm}3.2$) and pAEFs ($10.4{\pm}2.6$) did not differ significantly but was significantly (p<0.05) lower in pAEFs than that in IVF ($22.5{\pm}4.5$) embryos. Total cell number in pFFs ($28.4{\pm}4.3$) and pAEFs cloned blastocysts ($24.2{\pm}5.1$) was significantly (p<0.05) lesser than IVF control ($35.4{\pm}3.2$). Apoptosis rates between cloned blastocysts differed significantly (p<0.05) and were significantly (p<0.05) higher than IVF embryos. The blastocyst rates between the cloned embryos cloned with different cell passages did not differ significantly but in embryos cloned with 7-8 cell passage was significantly (p<0.05) lower than the IVF control. Apoptosis signals were detected in IVF and cloned embryos as early as day 3 and the rates of apoptosis increased concurrently with the embryo development. In conclusion, high apoptosis during in vitro preimplantation development resulted in low development rate and total cell number of cloned embryos. Moreover, based on the apoptotic incidence in cloned blastocysts, fetal fibroblasts are more suitable for production of cloned embryos in porcine.