• Archives of Reconstructive Microsurgery
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• 제4권1호
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• pp.1-8
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• 1995

장시간 허혈상태의 토끼 이개를 실험대상으로 하여 유로키나제와 헤파린을 병용 또는 단독으로 사용시와 또한 약물을 사용하지 않았을 때, 이들이 모세혈관의 개존성과 아울러 미세수술후의 조직 생존율에 미칠 수 있는 효과를 보기 위하여 허혈상태의 토끼 이개를 미세수술로 접합한 후 모세혈관으로의 혈류를 측정하기 위하여 레이저 초음파 혈류측정기(Laser doppler flowmetry)를 이용하였으며 방사선 구슬들(Cobalt-57 with plastic material with average diameter 15 micron)을 주입한 결과 유로키나제 조합에서 통계적으로 유효한 성적을 얻었다. 광학현미경 소견은 유로키나제와 헤파린을 병용한 조합에서 모세혈관내 내피세포의 배열이 유지되어 있었으며 헤파린을 사용한 조합에서도 유사한 소견을 보였으나 약물을 사용하지 않은 조합에서는 국소적인 내피세포의 배열이 결핍되어 있었다. 전자현미경 소견에서 유로키나제와 헤파린을 병용한 조합에서 내피세포가 혈관내벽에 배열되어 있었고 또 불규칙한 세포질이 돌출되어 있었다.

• 대한의용생체공학회:의공학회지
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• 제25권5호
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• pp.369-376
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• 2004

이명 재훈련 치료법은 백색 잡음을 이용하여 이명 환자를 치료하는 효과적인 방법이다. 이 치료법은 광대역의 청각 세포를 자극하기 위하여 백색 잡음을 이용한다. 본 논문에서는 열 잡음을 이용한 소형의 백색 잡음 발생기와 인간의 외이와 중이 시스템에 의해 변형된 주파수 응답을 보상할 수 있는 주파수 응답 조절부를 제안하였다. 실험 결과, 제안된 시스템이 기존의 백색 잡음 발생기에 비하여 이명 재훈련 치료의 목적에 보다 적합한 형태임을 알 수 있었다.

• 대한융합한의학회지
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• 제5권2호
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• pp.17-22
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• 2023

Objectives: This study aimed to investigate the anti-atopic properties of Glechoma hederacea var. longituba extracts (GHE) in murine atopic dermatitis model. Methods: BALB/c mouse ear stimulated with oxazolone (OX) for 4 weeks, then 1% GHE was topically applied every two days for 3 weeks to mouse. Ear thickness was measured by a digital thickness gauge. The ears tissues were collected and subjected to hematoxylin-eosin (H&E) and toluidine blue (TB) staining. Results: Treatment with GHE successfully alleviated the symptoms of atopic dermatitis, such as erythema, horny substance, and swelling. The infiltration of lymphocytes and mast cells were significantly reduced following GHE treatment. Conclusion: Taken together, our results showed that GHE possessed the potential to be a novel immunomodulatory drug against atopic dermatitis.

• Fibers and Polymers
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• 제6권3호
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• pp.219-223
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• 2005

Water-soluble chitin (WSC) was prepared by carefully deacetylating chitins to about $50\%$ of N-acetyl content. Topical formulations based on WSC were prepared and their effects on wound healing were evaluated on a rabbit ear model. Full-thickness, open skin wounds were made on the ears of rabbits and WSC ointments were embedded in the open wounds. The application of WSC ointments significantly accelerated wound healing and wound contraction. The areas of epithelial-ization and granulation tissues in WSC ointment group are remarkably larger than those in control group (no treatment) and in placebo group (treated with ointment-base materials). A large number of grown granulation tissues including dense fibroblast deposition were observed under the thickened epithelium of the wound treated with WSC ointments. The number of inflammatory cells in WSC ointment group was significantly decreased compared with those in control and placebo groups, indicating that WSC would give low stimuli to wounds and prevent excessive scar formation. Neovascularization was the most prominent in WSC ointment group. Wound contraction in WSC ointment group was much larger than those in control and placebo groups. Overall results demonstrate that the topical formulation based on WSC is considered to become an excellent dressing as a wound healing assistant.

• Archives of Pharmacal Research
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• 제29권6호
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• pp.503-507
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• 2006

Flavonoids are known as natural anti-inflammatory agents. In this investigation, an anti-inflammatory potential of new topical preparation (SK Ato $Formula^{\circledR}$) containing flavonoid mixtures from Scutellaria baicalensis Georgi roots and Ginkgo biloba L. leaves with an extract of Gentiana scabra Bunge roots was evaluated in an animal model of chronic skin inflammation. Multiple 12-O-tetradecanoylphorbol-13-acetate treatments for 7 consecutive days on ICR mouse ear provoked a chronic type of skin inflammation: dermal edema, epidermal hyperplasia and infiltration of inflammatory cells. When topically applied in this model, this row formulation $(5-20\;{\mu}L/ear/treatment)$ reduced these responses. Furthermore, it inhibited prostaglandin $E_2$ generation (17.1-33.3%) and suppressed the expression of proinflammatory genes, cyclooxygenase-2 and $interleulin-1{\beta}$ in the skin lesion. Although the potency of inhibition was lower than that of prednisolone, all these results suggest that Ato $Formula^{\circledR}$ may be beneficial for treating chronic skin inflammatory disorders such as atopic dermatitis.

• Parasites, Hosts and Diseases
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• 제51권1호
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• pp.69-74
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• 2013

Leishmania tropica is one of the causative agents of leishmaniasis in humans. Routes of infection have been reported to be an important variable for some species of Leishmania parasites. The role of this variable is not clear for L. tropica infection. The aim of this study was to explore the effects of route of L. tropica infection on the disease outcome and immunologic parameters in BALB/c mice. Two routes were used; subcutaneous in the footpad and intradermal in the ear. Mice were challenged by Leishmani major, after establishment of the L. tropica infection, to evaluate the level of protective immunity. Immune responses were assayed at week 1 and week 4 after challenge. The subcutaneous route in the footpad in comparison to the intradermal route in the ear induced significantly more protective immunity against L. major challenge, including higher delayed-type hypersensitivity responses, more rapid lesion resolution, lower parasite loads, and lower levels of IL-10. Our data showed that the route of infection in BALB/c model of L. tropica infection is an important variable and should be considered in developing an appropriate experimental model for L. tropica infections.

• 운동영양학회지
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• 제24권4호
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• pp.7-14
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• 2020

[Purpose] This study evaluated the anti-atopic dermatitis (AD) properties of Cordyceps militaris (CM) aqueous extract in keratinocytes in vitro and in vivo. We investigated the nutraceutical composition of the CM extract, including its protein, carbohydrate, and selected phytochemical content. [Methods] The expression of pathogenic cytokines in keratinocytes was assayed using an in vitro model. The CM extract downregulated extracellular signalregulated kinase 1/2 (ERK1/2) and p38 kinase expression in TNFα/IFNγ-stimulated HaCaT cells. We also established an in vivo AD model by repeatedly exposing the ears of mice to local Dermatophagoides farinae extract (DFE; house dust mite extract) and 2,4-dinitrochlorobenzene (DNCB). The epidermal and dermal ear thickness, mast cell infiltration, and serum immunoglobulin levels were measured following a 4-week oral administration of the CM extract. [Results] Histopathological examination showed reduced epidermal/dermal thickness and mast cell infiltration in mouse ears. The CM extract also suppressed serum immunoglobulin levels and gene expression of T helper (Th)1/Th2 cytokines in mouse ear tissue. [Conclusion] These results suggest that the CM extract may be useful for the treatment of AD-like skin lesions.

• 센서학회지
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• 제22권2호
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• pp.150-155
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• 2013

Recently, various hearing aids are developed to overcome hearing loss. There are available hearing aids, such as air conduction hearing aid, implantable middle ear hearing aid and so on. But air conduction hearing aid is inconvenience caused by howling, and ossicle chain driving type implantable middle ear hearing aid has some week point due to problem of possible nercobiosis of coupling spot along incus long process. In recent years, in order to improve these shortcomings round window (RW) driving hearing aid has been paying attention. In this paper, the physical cochlear model is proposed for a performance evaluation of the RW driving hearing aids of a transducer. In order to verify an experiment proposed on a performance of physical cochlear model, the transducer which has ossicles characteristics is used. By measuring and comparing the frequency characteristics of transducer with ossicles and human temporal bone, performance of physical cochlear model was verified. As from the result of experiment, it is expected that an implemented cochlear model is useful for evaluating characteristics of RW transducer.

• 동의생리병리학회지
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• 제20권1호
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• pp.196-201
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• 2006

Electroacupuncture(EA) is commonly used in various diseases. In the present study, the effect of EA in the allergic mouse model was examined. Allergy is generated via immunological mechanism and non-immunological mechanism. Mast cells activated dy those mechanisms get to release various substances such as histamine, leukotrienes, prostaglandin, TNF-$\alpha$, IL-4, IL-6, etc. which induce allergic reactions and the following inflammatory responses. To evaluate the anti-allergic effects of EA, mortality, ear swelling response, vascular permeability and cytokine secretion were investigated in EA group and non-EA group of which mice were compound 48/80-induced allergy model or PCA model. Compound 48/80 induces allergic reaction via non-immunological mechanism and PCA model is generated through the same mechanism with immediate-type(Type1) allergic reaction, one of immunological allergic reactions. EA inhibited compound 48/80-induced ear swelling response but did not inhibit the systemic anaphylaxis. EA also inhibited passive cutaneous anaphylaxis(PCA) activated dy anti-dinitrophenol IgE. In addition, EA inhibited IL-6 and TNF-$\alpha$ secretion from 48 h PCA in mice. These results indicate that EA may be used for the treatment of mast cell-mediated allergic diseases, especially immediate-type(Type 1) allergy and non-immunologically mediated allergy.

• 재활복지공학회논문지
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• 제5권1호
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• pp.59-64
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• 2011

인공 중이 (IMEHD, implantable middle ear hearing device)용 플로팅 매스 트랜스듀서는 제작 과정상에서 발생하는 구조적 오차 및 제작 결합시의 문제점들로 인해 설계 시 의도와 다른 진동 특성을 가질 수 있게 되고, 이렇게 제작된 진동 트랜스듀서는 향후 실제 환자에게 이식되었을 때의 진동체 진동 성능을 예측하기 어려운 문제점이 있다. 본 연구에서는 제작된 플로팅 매스형 트랜스듀서에 대해 설계값과의 비교 평가 및 향후 컴퓨터 모델화된 청각 중이 집중 소자 모델에 적용이 가능하도록 기 제작된 진동체의 집중 소자 모델 파라미터들을 추정할 수 있는 방법을 제안하였으며, 제안된 방법을 LabVIEW 기반의 그래픽 유저 인터페이스 소프트웨어로 구현하였다. 제안된 방법은 실제 제작된 플로팅 매스형 진동체의 진동 변위 및 위상 데이터를 이용하여 해당 진동체의 집중 모델 파라미터들을 순차적 이차 프로그래밍 (SQP, sequential quadratic programming) 방법으로 추정하는 방식이다. 구현된 방법을 이용하여 측정 데이터에 가해지는 잡음의 양에 따른 플로팅 매스형 진동 트랜스듀서의 집중 모델 파라미터 추정 시의 발생 오차 변화를 컴퓨터 시뮬레이션을 통해 조사하여 본 방법의 파라미터 추정 정확도를 확인하였다. 또한, 실제로 제작한 인공중이용 진동 트랜스듀서에 대해 측정된 진동 변위 및 위상 데이터를 이용하여 진동체의 집중 모델 파라미터들을 추정하였으며, 기존 설계값들과 비교를 통해 제안된 방법의 유용성을 평가하였다.