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Effect of Physical Control Technology on Aspergillus ochraceus Reduction (물리적 제어기술이 Aspergillus ochraceus 저감화에 미치는 영향)

  • Lee, Eun-Seon;Kim, Jong-Hui;Kim, Bu-Min;Oh, Mi-Hwa
    • Journal of Food Hygiene and Safety
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    • v.36 no.5
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    • pp.447-453
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    • 2021
  • In this study, the effectiveness of physical control technology, a combined light sterilization (LED, UV) and hot water treatment in reducing Aspergillus ochraceus for food production environment was investigated. In brief, 1 mL aliquot of A. ochraceus spore suspension (107-8 spore/mL) was inoculated onto stainless steel chips, which was then dried at 37℃, and each was subjected to different physical treatment. Treatments were performed for 0.5, 1, 2, 5, 8, and 11 hours to reduce the strains using a light-emitting diode, but no significant difference was confirmed among the treatments. However, a significant reduction was observed on the chips treated with UV-C exposure and hot water immersion. After being treated solely with 360 kJ/m2 of UV-C on stainless steel chip, the fungi were significantly reduced to 1.27 log CFU/cm2. Concerning the hot water treatment, the initial inoculum amount of 6.49 log CFU/cm2 was entirely killed by immersion in 83℃ water for 5 minutes. Maintaining a high temperature for 5 minutes at the site is difficult. Thus, considering economic feasibility and usability, we attempted to confirm the appropriate A. ochraceus reduction conditions by combining a relatively low temperature of 60℃ and UV rays. With the combined treatments, even in lukewarm water, A. ochraceus decreased significantly through the increases in the immersion time and the amount of UV-C irradiation, and the yield was below the detection limit. Based on these results, if work tools are immersed in 60℃ lukewarm water for 3 minutes and then placed in a UV sterilization device for more than 10 minutes, the possibility of A. ochraceus cross-contamination during work is expected to be reduced.

A Study on the Possibility of Short-term Monitoring of Coastal Topography Changes Using GOCI-II (GOCI-II를 활용한 단기 연안지형변화 모니터링 가능성 평가 연구)

  • Lee, Jingyo;Kim, Keunyong;Ryu, Joo-Hyung
    • Korean Journal of Remote Sensing
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    • v.37 no.5_2
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    • pp.1329-1340
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    • 2021
  • The intertidal zone, which is a transitional zone between the ocean and the land, requires continuous monitoring as various changes occur rapidly due to artificial activity and natural disturbance. Monitoring of coastal topography changes using remote sensing method is evaluated to be effective in overcoming the limitations of intertidal zone accessibility and observing long-term topographic changes in intertidal zone. Most of the existing coastal topographic monitoring studies using remote sensing were conducted through high spatial resolution images such as Landsat and Sentinel. This study extracted the waterline using the NDWI from the GOCI-II (Geostationary Ocean Color Satellite-II) data, identified the changes in the intertidal area in Gyeonggi Bay according to various tidal heights, and examined the utility of DEM generation and topography altitude change observation over a short period of time. GOCI-II (249 scenes), Sentinel-2A/B (39 scenes), Landsat 8 OLI (7 scenes) images were obtained around Gyeonggi Bay from October 8, 2020 to August 16, 2021. If generating intertidal area DEM, Sentinel and Landsat images required at least 3 months to 1 year of data collection, but the GOCI-II satellite was able to generate intertidal area DEM in Gyeonggi Bay using only one day of data according to tidal heights, and the topography altitude was also observed through exposure frequency. When observing coastal topography changes using the GOCI-II satellite, it would be a good idea to detect topography changes early through a short cycle and to accurately interpolate and utilize insufficient spatial resolutions using multi-remote sensing data of high resolution. Based on the above results, it is expected that it will be possible to quickly provide information necessary for the latest topographic map and coastal management of the Korean Peninsula by expanding the research area and developing technologies that can be automatically analyzed and detected.

Derivation of Green Infrastructure Planning Factors for Reducing Particulate Matter - Using Text Mining - (미세먼지 저감을 위한 그린인프라 계획요소 도출 - 텍스트 마이닝을 활용하여 -)

  • Seok, Youngsun;Song, Kihwan;Han, Hyojoo;Lee, Junga
    • Journal of the Korean Institute of Landscape Architecture
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    • v.49 no.5
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    • pp.79-96
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    • 2021
  • Green infrastructure planning represents landscape planning measures to reduce particulate matter. This study aimed to derive factors that may be used in planning green infrastructure for particulate matter reduction using text mining techniques. A range of analyses were carried out by focusing on keywords such as 'particulate matter reduction plan' and 'green infrastructure planning elements'. The analyses included Term Frequency-Inverse Document Frequency (TF-IDF) analysis, centrality analysis, related word analysis, and topic modeling analysis. These analyses were carried out via text mining by collecting information on previous related research, policy reports, and laws. Initially, TF-IDF analysis results were used to classify major keywords relating to particulate matter and green infrastructure into three groups: (1) environmental issues (e.g., particulate matter, environment, carbon, and atmosphere), target spaces (e.g., urban, park, and local green space), and application methods (e.g., analysis, planning, evaluation, development, ecological aspect, policy management, technology, and resilience). Second, the centrality analysis results were found to be similar to those of TF-IDF; it was confirmed that the central connectors to the major keywords were 'Green New Deal' and 'Vacant land'. The results from the analysis of related words verified that planning green infrastructure for particulate matter reduction required planning forests and ventilation corridors. Additionally, moisture must be considered for microclimate control. It was also confirmed that utilizing vacant space, establishing mixed forests, introducing particulate matter reduction technology, and understanding the system may be important for the effective planning of green infrastructure. Topic analysis was used to classify the planning elements of green infrastructure based on ecological, technological, and social functions. The planning elements of ecological function were classified into morphological (e.g., urban forest, green space, wall greening) and functional aspects (e.g., climate control, carbon storage and absorption, provision of habitats, and biodiversity for wildlife). The planning elements of technical function were classified into various themes, including the disaster prevention functions of green infrastructure, buffer effects, stormwater management, water purification, and energy reduction. The planning elements of the social function were classified into themes such as community function, improving the health of users, and scenery improvement. These results suggest that green infrastructure planning for particulate matter reduction requires approaches related to key concepts, such as resilience and sustainability. In particular, there is a need to apply green infrastructure planning elements in order to reduce exposure to particulate matter.

Characterizations of Water Quality, and Potential Relationships of Nitrogen Components and Microbes in the Mulgol Pond on Dokdo, Korea (독도 물골의 수질 특성 및 질소화합물과 미생물간의 잠재적 관계)

  • WOO, SANG YOON;LEE, HYEON BEEN;JEONG, DONG HYUK;AN, JE BAK;YOUN, JIN SUK;PAK, JAE-HONG;PARK, JONG SOO
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.26 no.2
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    • pp.124-134
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    • 2021
  • Water in the Mulgol pond on Dokdo (island), Korea, was historically used for drinking water, but now it has been no longer used for this purpose due to regionally low water quality. Since 2007, this pond has been covered with a metal lid to protect from pollutants of seabirds, indicating limited light penetration into the Mulgol pond. Here, we investigated water quality in the pond and potential relationships of nitrogen components and microbes in May, June, August, and November 2020. The source salinity ranged from 1.39 to 1.57 psu. Suspended solids (0.8~5.1 mg L-1) and chlorophyll-a (<0.01~0.49 ㎍ L-1) remained low. The concentration of dissolved inorganic nitrogen (DIN) was between 35.9 and 47.2 mg L-1. Thus, water in the Mulgol pond proves to be brackish water with low chlorophyll-a and high nutrients. This unique environment may be established by limited light intensity, sea fog (or seawater), and fecal pellets from many seabirds. Although the light source (800~8000 lux) was exposed to the four subsamples, chlorophyll-a concentrations were below <0.5 ㎍ L-1 during the incubation periods. This result suggests that the biomass of phytoplankton does not increase along with an increase in light intensity. Furthermore, the content of nitrate constituted more than 90% of DIN, and a significant negative correlation between nitrate concentration and bacterial abundance was shown in May and June 2020 during the light exposure experiments (R=-0.762, p<0.05). Thus, it is possible that bacteria may be a significant agent to reduce nitrate concentration in the Mulgol pond, the relationship between nitrate concentration and bacterial abundance may vary seasonally.

Correlation Analysis between Damage of Expansion Joints and Response of Deck in RC Slab Bridges (RC 슬래브교의 신축이음 손상과 바닥판 응답과의 상관관계 분석)

  • Jung, Hyun-Jin;An, Hyo-Joon;Park, Ki-Tae;Jung, Kyu-San;Kim, Yu-Hee;Lee, Jong-Han
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.25 no.6
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    • pp.245-253
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    • 2021
  • RC slab bridges account for the largest portion of deteriorated bridges in Korea. However, most RC slabs are not included in the first and second classes of bridges, which are subject to bridge safety management and maintenance. The highest damaged components in highway bridges are the subsidiary facilities including expansion joints and bearings. In particular, leakage through expansion joints causes deterioration and cracks of concrete and exposure of reinforced bars. Therefore, this study analyzed the effect of adhesion damage at expansion joints on the response of the deck in RC slab bridges. When the spacing between the expansion joints at both ends was closely adhered, cracks occurred in the concrete at both ends of the deck due to the resistance rigidity at the expansion joints. Based on the response results, the correlation analysis between displacements in the longitudinal direction of the expansion joint and concrete stress at both ends of the deck for each damage scenario was performed to investigate the effect of the occurrence of damage on the bridge behavior. When expansion joint devices at both sides were damaged, the correlation between displacement and stress showed a low correlation of 0.18 when the vehicles proceeded along all the lanes. Compared with those in the intact state, the deflections of the deck in the damaged case at both sides showed a low correlation of 0.34 to 0.53 while the vehicle passed and 0.17 to 0.43 after the vehicle passed. This means that the occurrence of cracks in the ends of concrete changed the behavior of the deck. Therefore, data-deriven damage detection could be developed to manage the damage to expansion joints that cause damage and deterioration of the deck.

Effect of 17β-estradiol on Ecdysteroid Pathway Related Genes in the Brackish Water Flea Diaphanosoma celebensis (17β-estradiol이 기수산 물벼룩의 Ecdysteroid 경로에 미치는 영향)

  • In, Soyeon;Yoo, Jewon;Cho, Hayoung;Lee, Young-Mi
    • Journal of Marine Life Science
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    • v.5 no.2
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    • pp.35-42
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    • 2020
  • 17β-estradiol (E2) is a natural hormone secreted by ovary, and continuously discharged from household and livestock wastewater into aquatic environment. Due to its strong estrogenic activity, it has adverse effects on development and reproduction in crustacean as an endocrine disrupting chemical. Although ecdysteroid signaling pathway play a key role in development in crustacean, little information on transcriptional modulation of ecdysteroid-related genes in response to E2 is available in small crustacean. Here, we investigated the acute toxicity of E2 to obtain 24-h LCx values in the brackish water flea Diaphanosoma celebensis. Time-dependent expression patterns of seven ecdysteroid pathway - related genes (CYP314a1, EcRA, EcRB, USP, ERR, Vtg, VtgR) were further examined using quantitative real time reverse transcriptase polymerase chain reaction (qRT-PCR). As results, 24-h LC50 and LC10 values were 9.581 mg/l and 4.842 mg/l, respectively. The mRNA expression of CYP314a1, EcRA, USP, VtgR was significantly up-regulated at 12 or 24 h after exposure to E2. These findings indicate that E2 can affect their molting and reproduction by modulating the expression of ecdysteroid pathway - related in D. celebensis. This study will be useful for better understanding of molecular mode of action of endocrine disrupting chemicals on molting process in small crustacean.

Protective effect of lycopene against cytokine-induced β-cell apoptosis in INS-1 cells (라이코펜이 사이토카인에 의해 유도된 베타세포 사멸에 미치는 효과 및 기전 연구)

  • Kim, Kyong;Jang, Se-Eun;Bae, Gong Deuk;Jun, Hee-Sook;Oh, Yoon Sin
    • Journal of Nutrition and Health
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    • v.51 no.6
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    • pp.498-506
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    • 2018
  • Purpose: Lycopene, a carotenoid with anti-oxidant properties, occurs naturally in tomatoes and pink grapefruit. Although the beneficial effects of lycopene on various disorders have been established, little attention has been paid to the possible anti-diabetic effects of lycopene focusing on ${\beta}$-cells. Therefore, this study investigated the potential of lycopene to protect ${\beta}$-cells against apoptosis induced by a cytokine mixture. Methods: For toxicity experiments, the cells were treated with 0.1 ~ 10 nM of lycopene, and the cell viability in INS-1 cells (a rat ${\beta}$-cell line) was measured using a MTT assay. To induce cytokine toxicity, the cells were treated with a cytokine mixture (20 ng/mL of $TNF{\alpha}$ + 20 ng/mL of IL-$1{\beta}$) for 24 h, and the effects of lycopene (0.1 nM) on the cytokine toxicity were measured using the MTT assay. The expression levels of the apoptotic proteins were analyzed by Western blotting, and the level of intracellular reactive oxidative stress (ROS) was monitored using a DCFDA fluorescent probe. The intracellular ATP levels were determined using a luminescence kit, and mRNA expression of the genes coding for anti-oxidative stress response and mitochondrial function were analyzed by quantitative reverse-transcriptase PCR. Results: Exposure of INS-1 cells to 0.1 nM of lycopene increased the cell viability significantly, and protected the cells from cytokine-induced death. Lycopene upregulated the mRNA and protein expression of B-cell lymphoma-2 (Bcl-2) and reduced the expression of the Bcl-2 associated X (Bax) protein. Lycopene inhibited apoptotic signaling via a reduction of the ROS, and this effect correlated with the upregulation of anti-oxidative stress response genes, such as GCLC, NQO1, and HO-1. Lycopene increased the mRNA expression of mitochondrial function-related genes and increased the cellular ATP level. Conclusion: These results suggest that lycopene reduces the level of oxidative stress and improves the mitochondrial function, contributing to the prevention of cytokine-induced ${\beta}$-cell apoptosis. Therefore, lycopene could potentially serve as a preventive and therapeutic agent for the treatment of type 2 diabetes.

Suitability of Migration Testing for Food Packaging Materials Using Tenax® (Tenax®를 이용한 식품포장재의 용출 실험의 적합성)

  • Kim, Hyeong-Jun;Bang, Dae Young;Kim, Min Ho;Lee, Keun Taik
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.24 no.3
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    • pp.97-106
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    • 2018
  • This study aimed at examining the suitability of $Tenax^{(R)}$ for the migration testing of food packaging materials, which is currently approved in the EU as a dry food simulant. The results are used as a basis to examine the feasibility of introducing $Tenax^{(R)}$ to Korean regulation. The OMVs of test specimen into various solvents (diethyl ether, ethanol, pentane, and acetone) after exposure to $100^{\circ}C$ for 1 hr were compared. Diethyl ether showed the highest OMV ($1.33mg/dm^2$) among the solvents tested. When the tests were conducted with different amounts of $Tenax^{(R)}$ of 2, 4, or 8 g per specimen, the OMVs were 0.75, 1.33 and $1.40mg/dm^2$, respectively. The OMV obtained with a closed system after wrapping with aluminum foil showed a significantly higher OMV ($1.61mg/dm^2$) than that without aluminum wrapping ($1.318mg/dm^2w$) and an open system without lid ($1.06mg/dm^2$). The specific migration rates of surrogates spiked in the polyethylene test film and paper samples into $Tenax^{(R)}$ were compared with those into liquid food simulants including 95% ethanol and n-heptane, and actual foods such as starch, skim milk, and sugar. In general, the specific migration levels of surrogates into $Tenax^{(R)}$ were similar compared with n-heptane, however those were significantly higher than into actual foods. These results suggest that $Tenax^{(R)}$ may be used as a food simulant for the long-term preservation of dried foods and paper products. However, more studies need to be conducted to investigate the factors influencing the migration into $Tenax^{(R)}$, such as the types of foods and packaging materials tested, migration conditions, and surrogates properties etc.

A Study on Vulnerability Assessment and Prioritizing Sectors to Support Adaptation Strategy to Climate Change - Case Study of Gangwon Province - (기후변화 적응대책 수립 지원을 위한 취약성 평가 및 부문별 우선순위 선정 방안 연구 - 강원도 사례를 중심으로 -)

  • Oh, Suhyun;Lee, Woo-Kyun;Yoo, Seongjin;Byun, Jungyeon;Park, Sunmin;Kwak, Hanbin;Cui, Guishan;Kim, Moonil;Jung, Raesun;Nam, Kijun;Shin, Donghee
    • Journal of Climate Change Research
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    • v.3 no.4
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    • pp.245-257
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    • 2012
  • Vulnerability assessment has been required for establish climate adaptation plan to prevent damage from climate change. In this study we assessed vulnerability with 1 km resolution and determined which sectors have the highest priority in each municipality of Gangwon province based on the result of vulnerability assessment. All sectors of vulnerability assessment are composed of three criteria; sensitivity, exposure and adaptation capacity. And suitable indicators of each sector were selected and spatial data set was prepared using GIS. Priority of vulnerability was classified with the degree of vulnerability in present and variation in vulnerability between present and future. The results of vulnerability assessment were different among municipalities due to the contribution of indicators. Present and future trends in vulnerability showed similar results but high vulnerable area was predicted to expand in the future. In addition increase in temperature led whole area to be more vulnerable generally. The result of prioritizing sectors of vulnerability indicated the most considerable sectors within a municipality. Also, the municipalities which have similar geographic, climatic and social conditions tended to be classified as the same priority class. The method of vulnerability assessment and determining priorities suggested in this study could be used to support decision makers to establish adaptation plan of local area.

A Study on Risk Factor Identification by Specialty Construction Industry Sector through Construction Accident Cases : Focused on the Insurance Data of Specialty Construction Worker (건설재해사례 분석에 의한 전문건설업종별 위험요인 탐색 : 전문건설업 근로자 공제자료를 중심으로)

  • Lee, Young Jai;Kang, Seong Kyung;Yu, Hwan
    • Journal of Korea Society of Industrial Information Systems
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    • v.24 no.1
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    • pp.45-63
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    • 2019
  • The number of domestic construction company is expanding every year while the construction workers' exposure to disaster risk is increasing due to technological advancements and popularity of high-rise buildings. In particular, the industry faces greater fatalities and severe large scale accidents because of construction industry characteristics including influx of foreign workers with different language and culture, large number of aged workers, outsourcing, high place work, heavy machine construction. The construction industry is labor-intensive, which is to be completed under given timeline and consists of unique working environment with a lot of night shifts. In addition, when a fixed construction budget is not secured, there is less investment in safety management resulting in poor risk management at the construction site. Taking account that the construction industry has higher accident risk rate and fatality rate, risky and unique working environment, and various labor pool from foreign to aged workers, preemptive safety management through risk factor identification is a mandatory requirement for the construction industry and site. The study analyzes about 8,500 cases of construction accidents that occurred over the past 10 years and identified risk factor by construction industry sector to secure a systematic insight for risk management. Based on interrelation analysis between accident types, work types, original cause materials and assailing materials, there is correlation between each analysis factor and work industry. Especially for work types, there is great correlation between work tasks and industry type. For reinforced concrete and earthwork are among the most frequent types of accidents, and they are not only high in frequency of accidents, but also have a high risk in categories of occurrence.