• 생명과학회지
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• 제18권9호
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• pp.1284-1289
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• 2008

본 연구에서는 EVOP-factorial system을 활용하여 매실식초의 최적 발효 조건을 찾고자 하였다. 3-7% 사이의 에탄올 농도(r=-0.5166)와 glucose 농도(r=-0.5061)는 10% 유의수준에서 산도에 영향을 미치는 것으로 확인되었다. 24-$33^{\circ}C$의 범위 내에서 발효온도는 매실식초의 산도 증가에 유의적인 영향을 미치지 못하는 것으로 확인되었다(r=0.1082). EVOP- factorial system을 활용하여 얻은 매실식초의 최적 발효조건은 발효 온도: $30^{\circ}C$, 에탄올 농도: 4%, 포도당 농도: 0.2%로 결정되었으며, 최적 조건에서의 산도값은 6.365%로 set 1의 중심점에서 나타난 산도값 5.4%에 비해 1.0%정도 높아졌다. 본 연구결과는 EVOP-factorial design을 이용하여 매실식초의 최적 발효 조건을 확인한 최초의 시도이다.

• Food Science and Biotechnology
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• 제17권5호
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• pp.899-903
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• 2008

This study was conducted in order to elucidate the optimum conditions for the extraction of clove that can be used to elicit antibacterial activity against Streptococcus mutans using the evolutionary operation (EVOP)-factorial design technique. Higher antibacterial activity was achieved in a higher extraction temperature of $80^{\circ}C$ ($r=0.7983^{**}$) and in a longer extraction time of 26 hr ($r=0.6867^*$). Antibacterial activity was not effected by differentiation of ethanol concentration in the extraction solvent (r=-0.0683). The maximum antibacterial activity of clove against S. mutans as determined by the EVOP-factorial design technique was obtained at an extraction temperature of $80^{\circ}C$, an extraction time of 26 hr and a 50% ethanol concentration. Furthermore, the population of S. mutans decreased from an initial concentration of 6.850 to 4.195 log CFU/mL in the third set that is more than 2.6 log cycles by EVOP-factorial design technique.

• Journal of Microbiology and Biotechnology
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• 제17권11호
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• pp.1880-1884
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• 2007

This study was conducted to find the optimum extraction condition of Gold-Thread for antibacterial activity against Streptococcus mutans using The evolutionary operation-factorial design technique. Higher antibacterial activity was achieved in a higher extraction temperature ($R^2=-0.79$) and in a longer extraction time ($R^2=-0.71$). Antibacterial activity was not affected by differentiation of the ethanol concentration in the extraction solvent ($R^2=-0.12$). The maximum antibacterial activity of clove against S. mutans determined by the EVOP-factorial technique was obtained at $80^{\circ}C$ extraction temperature, 26 h extraction time, and 50% ethanol concentration. The population of S. mutans decreased from 6.110 logCFU/ml in the initial set to 4.125 logCFU/ml in the third set.