• Reproductive and Developmental Biology
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• 제29권2호
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• pp.93-99
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• 2005

Human embryonic stem cells (hESCs) derived from the inner cell mass of blastocysts have the ability to renew themselves and to differentiate into cell types of all lineage. The present study was carried out to investigate whether the Wnt signaling pathway is related to maintaining self-renewal of hESCs. Glycogen Synthase Kinase 3 (GSK-3) inhibitor, BIO ((2'Z,3'E)-6-Bromoindirubin-3'-oxime) was treated to Miz-hES1 line for activation of Wnt signaling pathway. BIO-nontreated hESCs (control) and BID-treated hESCs were cultured for 5 days in the modified feeder-free system. During the culture of hESCs, differences were observed in the colony morphology between 2 groups. Controls were spread outwards whereas BIO-nontreated hESCs were clumped in the center and the differentiated cells were spreading outwards in the edges. The results of stem cell specific marker staining indicated that control were differentiated in large part whereas BIO-treated hESCs maintain self-renewal in the center of the colony. The results of lineage marker staining suggested that outer cells of the hESC colony were differentiated to the neuronal progenitor cells in both control and BIO-treated hESC. These results indicate that Wnt signaling is related to self-renewal in hESCs. In addition, control group showed higher composition of apoptotic cells $(23.76\%)$ than the BID-treated group $(5.59\%)$. These results indicate that BIO is effective on antapoptosis of hESCs.

• BMB Reports
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• 제54권9호
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• pp.464-469
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• 2021

Cardiomyocyte differentiation occurs through complex and finely regulated processes including cardiac lineage commitment and maturation from pluripotent stem cells (PSCs). To gain some insight into the genome-wide characteristics of cardiac lineage commitment, we performed transcriptome analysis on both mouse embryonic stem cells (mESCs) and human induced PSCs (hiPSCs) at specific stages of cardiomyocyte differentiation. Specifically, the gene expression profiles and the protein-protein interaction networks of the mESC-derived platelet-derived growth factor receptor-alpha (PDGFRα)⁺ cardiac lineage-committed cells (CLCs) and hiPSC-derived kinase insert domain receptor (KDR)⁺ and PDGFRα⁺ cardiac progenitor cells (CPCs) at cardiac lineage commitment were compared with those of mesodermal cells and differentiated cardiomyocytes. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that the genes significantly upregulated at cardiac lineage commitment were associated with responses to organic substances and external stimuli, extracellular and myocardial contractile components, receptor binding, gated channel activity, PI3K-AKT signaling, and cardiac hypertrophy and dilation pathways. Protein-protein interaction network analysis revealed that the expression levels of genes that regulate cardiac maturation, heart contraction, and calcium handling showed a consistent increase during cardiac differentiation; however, the expression levels of genes that regulate cell differentiation and multicellular organism development decreased at the cardiac maturation stage following lineage commitment. Additionally, we identified for the first time the protein-protein interaction network connecting cardiac development, the immune system, and metabolism during cardiac lineage commitment in both mESC-derived PDGFRα⁺ CLCs and hiPSC-derived KDR⁺PDGFRα⁺ CPCs. These findings shed light on the regulation of cardiac lineage commitment and the pathogenesis of cardiometabolic diseases.

• International Journal of Stem Cells
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• 제17권1호
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• pp.59-69
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• 2024

Human pluripotent stem cells (hPSCs) such as human embryonic stem cells (hESCs), induced pluripotent stem cells, and somatic cell nuclear transfer (SCNT)-hESCs can permanently self-renew while maintaining their capacity to differentiate into any type of somatic cells, thereby serving as an important cell source for cell therapy. However, there are persistent challenges in the application of hPSCs in clinical trials, where one of the most significant is graft rejection by the patient immune system in response to human leukocyte antigen (HLA) mismatch when transplants are obtained from an allogeneic (non-self) cell source. Homozygous SCNT-hESCs (homo-SCNT-hESCs) were used to simplify the clinical application and to reduce HLA mismatch. Here, we present a xeno-free protocol that confirms the efficient generation of neural precursor cells in hPSCs and also the differentiation of dopaminergic neurons. Additionally, there was no difference when comparing the HLA expression patterns of hESC, homo-SCNT-hESCs and hetero-SCNT-hESCs. We propose that there are no differences in the differentiation capacity and HLA expression among hPSCs that can be cultured in vitro. Thus, it is expected that homo-SCNT-hESCs will possess a wider range of applications when transplanted with neural precursor cells in the context of clinical trials.

• 한국유기농업학회지
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• 제9권4호
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• pp.53-69
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• 2001

The word \"permaculture\" is a contraction of \"permanent agriculture\" or \"permanent culture\". Permaculture principles are designed to support (or ensure) the survival of humanity, the earth and all on it, and, to improve our standard of living, Permaculture can be defined as : a design system for creating sustainable human environments that can be (relatively) easily constructed and maintained. The principles of permaculture designs are relative location every element (such as house , rice paddy, road, esc) is placed in relation to each other such that each assists the other : each element performs multiple functions ; each function is supported by many elements : energy efficient planning ; using biological resources rather than fossil fuels : energy cycling on site (both fuel and human energy) : Using and accelerating natural plant succession to establish favourable sites and soils ; poly-culture and diversity of beneficial species for a productive, interactive system ; use of edge and natural patterns for best effect. These principles can be used for any permaculture design, in any climate, and at any scale. There has been very little scientific examination of Permaculture, and as such, it must be viewed as a design philosophy.viewed as a design philosophy.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2004년도 추계학술대회 논문집
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• pp.1185-1192
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• 2004

Recently heavy traffic is getting worse because increasing population of transportation in urban area. In order to solve this problem. subway is realized with high speed, high density, highly efficiency. When fire accident happened in Dae-Gu subway in February 2003, there happened loss of people and lots of damage because of not being able to control even though fire alarm which was set up in the station rang. This thesis has constructed a simulation integrating operation system using a Database construction, operating program analysis in order to build up the most efficient train operation system. The result of simulation integrating operation system with emergency virtual situation like station and train fire, train failure, power line failure, all trains running on the rail were secured safety by train operation control system. With integrating operation of each train control system, train system, power system, machine facility system, the most efficient integrating operation system should have been constructed at the time the subway fire and power failure broke out.

• 한국자동차공학회논문집
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• 제24권4호
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• pp.439-446
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• 2016

An in-wheel motor vehicle is a type of car that is equipped with an electric motor for each wheel. It is possible to acquire vehicle stability through a seperate driving torque control per wheel, since it directly generates the driving torque via the wheel motors. However, the vehicle ride comfort and road holding performance worsen depending on the increase of the wheel weights. In order to compensate for the impaired performance, an integrated chassis control system of the rear in-wheel motor vehicle is proposed. The proposed integrated chassis control system is composed of a driving torque control system, a semi-active suspension system, and an ESC system. According to the vehicle dynamic simulation of an in-wheel motor vehicle equipped with the integrated chassis control system, it is found that the system can improve the driving stability, ride comfort, and driving efficiency of the in-wheel motor vehicle.

• 제어로봇시스템학회논문지
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• 제16권7호
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• pp.646-654
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• 2010

This paper describes an integrated chassis control for a maneuverability, a lateral stability and a rollover prevention of a vehicle by the using of the ESC and AFS. The integrated chassis control system consists of a supervisor, control algorithms and a coordinator. From the measured and estimation signals, the supervisor determines the vehicle driving situation about the lateral stability and rollover prevention. The control algorithms determine a desired yaw moment for lateral stability and a desired longitudinal force for the rollover prevention. In order to apply the control inputs, the coordinator determines a brake and active front steering inputs optimally based on the current status of the subject vehicle. To improve the reliability and to reduce the operating load of the proposed control algorithms, a multi-core ECU platform is used in this system. For the evaluation of this system, a closed loop simulations with driver-vehicle-controller system were conducted to investigate the performance of the proposed control strategy.

• 한국음향학회:학술대회논문집
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• 한국음향학회 2000년도 하계학술발표대회 논문집 제19권 1호
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• pp.95-98
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• 2000

음성 인식 시스템의 개발을 위해서는 음성 데이터베이스 구축이 중요한 과제의 하나로써, 많은 시간과 노력이 요구된다. 본 논문은 ARS 주식거래 시스템에서 사용되는 주식의 매수, 매도, 증시 현황에 관련된 문장과 숫자음에 대하여 DB 구축한다. 이 DB 구축을 위하여 Dialogic 사의 D/41ESC보드를 장착하고, Window NT4.0 플렛폼에서 음성을 수집하였다. 본 논문에서는 음성 수집을 위해 전국의 20대에서 50대까지의 남녀에 대해 1명당 50개의 문장 또는 숫자음에 대하여, 유선 및 무선을 통하여 데이터를 수집하였다. 또한 화자 독립 음성 인식을 위하여 1200명의 화자로 구성되어 있다. 지역별로 보면, 서울 및 경기, 강원 지역과 영호남, 충청 지역으로 나누었으며, 일반폰, 휴대폰, 공중전화의 환경에서, 그리고 실내와 실외환경에서 각각 수집하였다.

• Reproductive and Developmental Biology
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• 제38권4호
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• pp.137-142
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• 2014

A tremendous increase in the human population has put poultry industry under an increased pressure to meet steep increase in the demand. Poultry is contributing 25% of the total world's meat production and lesser cost of investment per bird makes it more suitable for the further breeding programmes. Major poultry diseases frequently lead to cardiac damage and cause huge economic losses to poultry industry due to mortality. The in vitro embryonic stem cell (ESC) technology has a futuristic approach for homogeneous populace of differentiated cells, for their further transplantations. During in vitro conditions the differentiated cell populace can be used in grafting and transplantation processes to regenerate damaged tissues. Therefore, the current study targeted the use of spermatogonial stem cells (SSCs) in the poultry production system through cardiac regeneration. The current study will also open new boulevard for the similar kind of research in other livestock species for the management of heart diseases.

• 한국자동차공학회논문집
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• 제24권3호
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• pp.310-318
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• 2016

The demands for vehicle safety systems such as ABS and ESC have been increased. Accurate vehicle state estimation is required to realized the abovementioned systems and tire-friction coefficient is crucial information. Estimation of lateral tire-road friction coefficient using pneumatic trail information is mainly dealt in this paper. Pneumatic trail shows unique characteristics according to the wheel side slip angle and these property is highly sensitive to vehicle lateral motion. The proposed algorithm minimizes the use of conventional tire models such as magic formula, brushed tire model and Dugoff tire model. The pure side slip maneuver, which means no longitudinal dynamics, is assumed to achieve the ultimate goal of this paper. A simulation verification using Carsim and Simulink is performed and the results show the feasibility of the proposed algorithms.