• Molecules and Cells
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• 제37권12호
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• pp.899-906
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• 2014

Prostaglandin $E_2$ ($PGE_2$) promotes tumor-persistent inflammation, frequently resulting in cancer. Curcumin is a diphenolic turmeric that inhibits carcinogenesis and induces apoptosis. $PGE_2$ inhibits curcumin-induced apoptosis; however, the underlying inhibitory mechanisms in colon cancer cells remain unknown. The aim of the present study is to investigate the survival role of $PGE_2$ and whether addition of exogenous $PGE_2$ affects curcumininduced cell death. HCT-15 cells were treated with curcumin and $PGE_2$, and protein expression levels were investigated via Western blot. Reactive oxygen species (ROS) generation, lipid peroxidation, and intracellular glutathione (GSH) levels were confirmed using specific dyes. The nuclear factor-kappa B ($NF-{\kappa}B$) DNA-binding was measured by electrophoretic mobility shift assay (EMSA). $PGE_2$ inhibited curcumin-induced apoptosis by suppressing oxidative stress and degradation of PARP and lamin B. However, exposure of cells to the EP2 receptor antagonist, AH6809, and the PKA inhibitor, H89, before treatment with $PGE_2$ or curcumin abolished the protective effect of $PGE_2$ and enhanced curcumin-induced cell death. $PGE_2$ activates PKA, which is required for cAMP-mediated transcriptional activation of CREB. $PGE_2$ also activated the Ras/Raf/Erk pathway, and pretreatment with PD98059 abolished the protective effect of $PGE_2$. Furthermore, curcumin treatment greatly reduced phosphorylation of CREB, followed by a concomitant reduction of $NF-{\kappa}B$ (p50 and p65) subunit activation. $PGE_2$ markedly activated nuclear translocation of $NF-{\kappa}B$. EMSA confirmed the DNA-binding activities of $NF-{\kappa}B$ subunits. These results suggest that inhibition of curcumin-induced apoptosis by $PGE_2$ through activation of PKA, Ras, and $NF-{\kappa}B$ signaling pathways may provide a molecular basis for the reversal of curcumin-induced colon carcinoma cell death.

• 한국환경보건학회지
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• 제42권1호
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• pp.53-60
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• 2016

Objectives: Preservatives are commonly used in pharmaceuticals, cosmetics and other products to extend the expiration date and prevent the growth of microorganisms. Preservatives are generally effective in controlling mold and inhibiting yeast growth, and against a wide range of bacterial attacks as well. They also adversely affect the quality of sperm and cause precocious puberty in children. This study was performed to analyze seven preservatives used in pharmaceuticals and personal care products. Methods: Five kinds of pharmaceuticals and personal care products (PPCPs) were examined for analysis with a high performance liquid chromatography-diode array detector. Each sample was homogenized and the targeted compounds were extracted with methanol. The suspended particulate was removed by syringe filter. Next, the sample was injected into an HPLC system. The separation of the seven preservatives was achieved with a C18 column and gradient mode. The accuracies were between 73% and 120% and precision was lower than 11.58% (RSD). Results: All of the calibration curves showed good linearity with a coefficient of determination ($r^2$) over 0.999. Among the PPCP samples, the detection rate of preservatives was 32.5% for pharmaceuticals, 44.8% for toothpaste, 76.9% for mouthwash, 40.0% for body lotion and 56.0% for wet tissues. The average concentrations of the preservatives in PPCPs were BA 1141.0 mg/kg, MP 709.8 mg/kg, EP 624.9 mg/kg, PP 216.9 mg/kg, BP 167.8 mg/kg, and TCS 538.2 mg/kg. The most frequently detected preservatives in pharmaceuticals and personal care products were BA, MP and PP. The concentrations of preservatives exceeded Korean regulatory standards in 11 samples of medicines, three of mouthwash and two of body lotion. Conclusion: We found that most of the PPCP samples contained various preservatives. It is necessary to identify which preservatives were used and to determine the level of preservatives in PPCPs and to assess the health risk to susceptible populations such as children.

• 한국환경보건학회지
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• 제38권6호
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• pp.561-567
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• 2012

Objectives: Parabens are widely used as antimicrobial agents in pharmaceuticals and cosmetics as well as by the food industry. Parabens have been reported to show weak estrogenic activity and be related to health effects such as allergic reactions and skin and breast cancer. We evaluated an online solid phase extraction (SPE) method coupled with LC-MS/MS technique using free and conjugated parent parabens in human urine for assessing human exposure to parabens. Methods: We employed LC/MS/MS through online solid phase extraction and column-switching techniques and analyzed free and conjugated parabens as biomarkers of human exposure. Four major parabens, methyl-paraben (MP), ethyl-paraben (EP), propyl-paraben (PP) and butyl-paraben (BP), were analyzed. Method validation was performed by sensitivity, accuracy, precision and comparison of the results of online SPE with offline SPE. Results: The limits of detection (LOD) were in the range of 0.2-2 ng/mL, and actual limits of quantification (LOQ) were in the range of 0.7-6 ng/mL urine, depending upon the compound. Accuracy was in the range of 98.3-106.4%, and precision was in the range of 1.3-8.7% (CV) depending upon the compound. We found a good correlation between the results of analysis by online SPE method and that by off-line SPE method. Conclusions: The online SPE method showed proper LOD and validated accuracy, precision and good correlation with the offline method for analyzing parabens in urine.

• Advances in environmental research
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• 제2권1호
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• pp.9-17
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• 2013

Urine is a widely used matrix in biomonitoring studies on the assessment of human exposure to environmental chemicals such as phthalate esters and bisphenol A (BPA). In addition to the need to apply valid analytical techniques, assurance of specimen integrity during collection and storage is an important prerequisite for the presentation of accurate and precise analytical data. One of the common issues encountered in the analysis of non-persistent contaminants is whether shipping and storage temperature and time since collection have an effect on sample integrity. In this study, we investigated the stability of phthalate metabolites and BPA in spiked and unspiked urine samples stored at room temperature ($20^{\circ}C$) or at $-80^{\circ}C$ for up to 8 weeks. Concentrations of phthalate metabolites declined, on average, by 3% to 15%, depending on the compounds, and BPA declined by ~30% after 4 weeks of storage of spiked urine samples at $20^{\circ}C$. In a test of 30 unspiked urine samples stored at $20^{\circ}C$ and at $-80^{\circ}C$ for 8 weeks, the concentrations of phthalate metabolites and BPA decreased by up to 15% to 44%, depending on the compound and on the samples. It was found that the small reduction in phthalate concentrations observed in urine, varied depending on the samples. In a few urine samples, concentrations of phthalate metabolites and BPA did not decline even after storage at $20^{\circ}C$ for 8 weeks. We found a significant relationship between concentrations of target analytes in urine stored at $20^{\circ}C$ and at $-80^{\circ}C$ for 8 weeks. We estimated the half-lives of phthalate metabolites and BPA in urine stored at $20^{\circ}C$. The estimated half-life of monoethyl phthalate (mEP) and mono (2-ethyl-5-carboxyphentyl) phthalate (mECPP) in urine stored at $20^{\circ}C$ was over two years, of mono (2-ethyl-5-oxohexyl) phthalate (mEOHP) and monobenzyl phthalate (mBzP) was approximately one year, and of other phthalate metabolites was approximately 6 months. The estimated half-life of BPA in urine stored at $20^{\circ}C$ was approximately 3 months, which is much longer than that reported for aquatic ecosystems.

• Asian-Australasian Journal of Animal Sciences
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• 제18권5호
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• pp.671-676
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• 2005

A total of 50 individual catfish, the Tra (Pangasius hypophthalmus) cultivated in either floating cages (Tra-c) or in ponds (Tra-p) and the Basa (Pangasius bocourti) raised in three floating cages, were collected in two of the Mekong Delta provinces. The caudal fin of each individual fish was used for protein electrophoresis employing the SDS-PAGE method. The one fillet sides were used as a representative sample to determine the dry matter (DM), crude protein (CP), ether extract (EE) and amino acids (AAs). The catfish oil was extracted from the belly fats, and the fatty acid (FA) composition was analyzed. There were 21 bands of the Tra and the Basa. Protein bands of the two varieties were 28.6-33.3% polymorphic, while polymorphic individuals of the Tra ranged from 80.0 to 100.0%, and the Basa was 90.0% polymorphic. The phenotypic diversity (Ho) of the Tra ranged from 1.71 to 1.80, while the Basa ranged as high as 2.14%. Diversity values (H$_{EP}$) for genetic diversity markers were equal in the Tra and the Basa. The sum of the effective number of alleles (SENA) of both varieties ranged from 3.40 to 3.83 for the Basa and the Tra, respectively. The lower values of Ho and SENA, as compared with those of the fresh water prawn (Macrobrachium equidens) in the area, would suggest that the species with the low values will become extinct due to inbreeding; the gene pools of each observed population were below a suitable threshold. Many of the differences in the nutritional values of the Tra-c, the Tra-p and the Basa were measured; their nutrient values were comparable to fishmeal or fish oil. Most of the DM, CP, and EE were higher in the Tra, especially in the Tra-c. The essential AA content, especially that of lysine, was highest in the Tra-c, next highest in the Tra-p, and lowest in the Basa. Therefore, the amino acid patterns were closer to the ideal patterns in the same sequences. In contrast, the essential FAs were concentrated in the Basa fish oil. It was found that suitable selection of parents for seed production is required to avoid inbreeding. Catfish may be valuable sources of nutrition for both humans and animals, and the differences in their nutritional values by variety and/or management must be taken into account.

• 자원리싸이클링
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• 제25권2호
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• pp.17-24
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• 2016

본 연구에서는 폐소형가전 재활용을 위한 파쇄, 선별 및 회수 공정에 대해 전과정평가(Life Cycle Assessment, LCA) 기법을 활용하여 환경영향을 분석하였다. 폐소형가전 파쇄, 선별 및 회수 공정의 지구온난화(Global Warming Potential, GWP)에 대한 환경영향은 $1.29E+02kg\;CO_2-eq./kg$으로 분석되었으며, 가중치 결과 1.17E-02Pt로 지구온난화(GWP)에 대한 환경영향의 경우 전체 환경영향의 약 57.1%, 자원고갈(ADP) 약 35.4%, 광화학산화물 형성(POCP) 약 4.8% 등이었다. 유용자원 1톤을 선별/회수하는 공정의 지구온난화(GWP)에 대한 환경영향은 플라스틱류 즉, ABS 33.7%, PP와 HIPS가 각각 약 16.8%, 철금속류 약 9.4% 등으로 플라스틱류의 선별/회수공정의 경우 지구온난화에 대한 환경영향이 가장 큰 것으로 분석되었다. 또한, 유용자원을 회수하여 재활용하므로서 신재 생산보다 지구온난화(GWP) 환경영향에 대하여 약 $2.73E+04ton\;CO_2-eq./ton$의 환경회피 효과가 발생하였으며, 이는 연간 약 48,236.4천원의 경제적 효과가 발생하는 것으로 조사되었다. 폐소형가전으로부터 유용자원을 효율적으로 재활용할 경우 지구온난화(GWP)에 대한 환경영향을 줄여 기후변화 대응에 효과적일 것으로 사료된다.

• 한국산학기술학회논문지
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• 제18권12호
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• pp.57-64
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• 2017

오늘날 EU환경규제로 포장용 완충재로 사용되는 EPS(스티로폼) 대체용 공기 충진 완충재는 출시 이후 점진적으로 시장이 확대되는 포장업계 블루칩이다. 공기충진형 완충재는 뛰어난 완충능력과 친환경성으로 EPS를 대체하여 빠르게 시장을 확장하고 있다. 기존 공기 충진 완충재에 대비하여 원료절감과 공정향상, 직관적 미관 향상을 모두 충족하는 새로운 선택 충진형 공기 충진재 제조기술이다. 본 연구는 다단 에어셀 충진 밸브성형기술은 선택충전 기술을 바탕으로, 밸브형성 구조기술을 적용하여 선택적으로 다양한 형태로 충진이 가능한 기술이다. 다단 에어셀 충진 밸브성형기술의 구조도는 밸브단이 구분된 제1주입필름, 밸브필름, 제2주입필름 등 3겹의 필름을 적층하여 복수의 주입구를 형성한 기술이다. 기존의 기술은 연결된 다수의 공기주머니에 공기를 주입하기 위한 별도의 외부 공기주입로가 필수적으로 구성되어야 하나, 본 연구는 공기 주머니의 내부에 외부 공기주입로가 형성되어 공기를 주입하기 위한 외부 공기주입로가 외견상 없어짐으로써 그에 따른 원재료 및 공정이 감소하고, 공기가 주입되어 원형 튜브로 팽창하는 과정에서 단면의 길이가 63~66%로 축소되는 공기주머니와 다르게 공기주입 후 내부 공기가 배출되고 단면이 원래의 길이를 유지하여 불필요하게 접힌 채 붙어 있는 외부 공기주입로를 내부에 일체화 하여 완충재의 미관이 향상되는 기술적 특징과 다수의 연속된 공기주머니에 선택적으로 공기를 충전할 수 있는 선택충전에 관한 것으로 기존 기술대비 뛰어난 차별성을 갖는 스마트한 공기충전형 완충재 제조 기술이다.

• 암석학회지
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• 제3권3호
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• pp.185-195
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• 1994

순수하게 제조한 합성광물을 시작 물질로 하여 석류석과 녹염석간의 철.알루미늄 교환반응에 대한 고온.고압 실험 결과, 분배계수 $K_D$와 온도 및 압력과의 상관관계가 밝혀졌다. 이는 석류석과 녹염석의 공존관계가 지질 온도계로서 역할을 할 수 있다는 것을 뒷받침해 주는 주요한 의미를 갖게 되는 것이다. 지금까지 녹염석이 관여 되어 있는 화학 반응의 실험적 연구는 녹염석 고용체의 순수한 단성분이나 그 고용체의 합성이 불가능하였고 자연산 녹염석을 사용하였거나, 다른 여러 광물상에 부수적으로 생성된 합성 녹염석을 단편적으로 사용하였기 때문에 석류석.녹염석 지질온도계가 수립될 수 없었다. 2Kb, 4Kb, 10Kb에서 석류석.녹염석간의 철.알루미늄 분배계수($K_D$)와 온도와의 관계를 각각 도식화 하였으며 온도는 압력에 비하여 $K_D$값에 매우 큰 영향을 미친다. 즉 온도가 증가함에 따라 $Fe^{+3}$는 석류석에서 이동하여 녹염석으로 재 배치되며, 반대로 $Al^{+3}$는 녹염석에서 나와 석류석으로 들어간다. 이는 온도가 증가함으로써 금속원소인 알루미늄이 전기적으로 양성이 되어 수화광물이며 단사정계에 속한 녹염석에서 일출되어 무수광물이며 등축정계에 속하는 석류석으로 재 배치되는 것이다. 압력은 온도에 비하여 석류석.녹염석 지질온도계에 심대한 영향을 주지는 못 한다. 그러나 $500^{\circ}C$에서 압력이 증가함에 따라 $K_D$의 값이 약간 감소하는 경향을 보여 준다. 이는 압력의 증가가 미미하지만, 온도 증가의 경우와는 반대로, $Fe^{+3}$이 녹염석에서 일출되어 석류석으로 재 배치 된다는 것을 의미한다.

• 한국균학회지
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• 제37권1호
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• pp.91-95
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• 2009

자연에서 채집한 치마버섯 자실체로부터 분리한 Schizophyllum commune 균을 배양하여 얻은 균사체 배양물로부터 추출한 다당류의 물리화학적 특성에 대하여 조사하였다. 액체배양 균사체로부터 추출된 다당류는 방법에 따라 $0.19{\sim}1.63%$의 수율을 나타내었다. 이들 다당류의 총 당은 $48.9{\sim}72.0%$이고, 단백질은 $15.3{\sim}32.0%$를 함유하고 있는 것으로 나타났다. 추출한 SC-EP 다당류의 구성 당은 glucose가 69.6%로 주를 이루고, fructose가 26.1%, 소량의 xylose로 구성된 것으로 나타나는 것으로 보아 hetero 다당류인 것으로 추정되었다. 또한, 산성 아미노산인 aspartic acid, glycine 및 glutamic acid 등은 각각 12.5%, 18.1% 및 15.2%, 중성아미노산인 alanine은 9.9%, 당과 단백질의 O${\beta}-glycosidic$ 결합에 관여하고 있는 threonine과 serine의 경우는 2.9% 및 2.7%로 나타났다. IR 분석결과 이들은 ${\alpha}$ 와 ${\beta}$-glucan이 혼재된 다당류인 것으로 나타났다. 한외여과장치로 제조한 다당류(SC-UP)의 0.5% 용액의 점도는 20 rpm 시 70 cps로 나타났다. 이상의 치마버섯 액체배양 균사체를 열수추출하여 에탄올 침전시켜 얻은 다당류는 당과 단백질이 결합된 단백다당류의 결합 형태를 이루고, 당은 2가지 이상의 당이 혼재하고 있으며, 아미노산은 산성아미노산의 함량이 높은 것으로 나타났다. 다당류의 결합 양식은 ${\alpha}$ 와 ${\beta}$-glucan이 혼재하고 있으며, 강한 점성을 나타내었다.

• 한국지반공학회논문집
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• 제29권11호
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• pp.61-72
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• 2013

Sabkha층 탄산질 모래의 입자파쇄발생시 이차압축특성을 파악하기 위하여 압밀시험을 수행하여 입자파쇄응력을 파악하였다. 입자파쇄 항복응력에서 순간적으로 발생한 입자파쇄 압축침하를 해석하기 위하여 이차압축률 대신 이차 파쇄압축률 $C_{{\alpha}{\epsilon}}{^*}$를 도입하였다. 입자파쇄 발생시의 간극비 $e_p{^*}$와 침하량 $H_p{^*}$가 이차압축거동의 기준점으로 사용되었다. 일차압축지수와 이차파쇄압축률을 비교한 결과 현장 Sabkha층의 경우 $C_{{\alpha}{\epsilon}}{^*}/C_c$ 값이 0.0105~0.0187로 나타났으며 일반적인 석영질 사질토에 비해 입자파쇄 발생으로 인한 이차압축거동이 매우 크게 나타났다. 동일한 재하응력에서는 Sabkha층의 심도가 깊을수록 입자파쇄침하량과 이차파쇄압축률이 증가함을 확인할 수 있었다.