• 제목/요약/키워드: EISA

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Monoclonal antibody-based enzyme immuno-slide assay (EISA) in the rapid diagnosis of Peste des petits ruminants of goats

  • Das, Kamol K.;Rahman, M.B.;Shil, N.K.;Rahman, Md Siddiqur;Jang, Hyung-Kwan;Song, Hee-Jong
    • Korean Journal of Veterinary Service
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    • v.33 no.1
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    • pp.1-6
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    • 2010
  • Monoclonal antibody (mAb)-based enzyme immune-slide assay (EISA) was used for the detection of Peste des petits ruminants (PPR) virus from field samples collected from a natural outbreak. The clinicopathological study was undertaken to diagnose the case primarily of PPR. Antigen was detected from discharges and faeces of infected goats and swabs of postmortem lesions prepared on glass slide or glass plate using acetone fixation. Nasal discharge collected at the early stage of disease course or lung is an appropriate ante- or postmortem sample for this technique, respectively. Convalescent polyclonal sera collected from recovered animals which were diagnosed as PPR by EISA showed high antibody titer against PPR by C-ELISA, demonstrating the satisfactory specificity of the test. Therefore, EISA is a sensitive and specific assay to confirm PPR infection both in field and laboratory conditions and especially suitable for developing country.

Derivation of Cubic and Hexagonal Mesoporous Silica Films by Spin-coating

  • Pan, Jia-Hong;Lee, Wan-In
    • Bulletin of the Korean Chemical Society
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    • v.26 no.3
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    • pp.418-422
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    • 2005
  • By introducing spin-coating method to the evaporation induced self-assembly (EISA) process, a simple and reproducible route in controlling the mesophase of silica thin films has been developed for the first time in this work. When a comparatively solvent-rich Si-sol (The atomic ratio of TEOS : F127 : HCl : $H_2O$ : EtOH = 1 : 0.006 : 0.2 : 9.2 : 30) was used as coating solution, the mesophase of resultant silica films was selectively controlled by adjusting the spin-on speed. The cubic mesophase has been obtained from the coating at a low rpm, such as 600 rpm, while the 2-D hexagonal mesophase is formed at a high rpm, such as 2,500 rpm. At a medium coating speed, a mixture of cubic and hexagonal mesophase has been found in the fabricated films. The present results confirm that the evaporation rate of volatile components at initial step is critical for the determination of mesopore structures during the EISA process.

Design of Traffic Data Acquisition System with Loop Defector and Piezo-Electric Sensor (루프검지기와 피에조 센서를 이용한 차량정보 수집 시스템 설계)

  • 한경호;양승훈
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.16 no.6
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    • pp.102-108
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    • 2002
  • This paper handles the design of a real time traffic data acquisition system using loop detector and piezo-electric sensor to acquire the vehicle information EISA compatible parallel I/O interface card is designed to sample 30 I/O channels at variable rates for raw traffic data acquisition. The control software is designed to generate the traffic data informations from the raw data. The traffic data information provides vehicle length, speed, number of axles, etc. Vehicle types are detected and categorized into eleven types from the vehicle length, axles positions and axle counts information. The traffic information is formed into packet and transferred to the remote hosts through serial communications for ITS applications.

MODIFIED NUMEROV METHOD FOR SOLVING SYSTEM OF SECOND-ORDER BOUNDARY-VALUE PROBLEMS

  • Al-Said, Eisa A.;Noor, Muhammad Aslam
    • Journal of applied mathematics & informatics
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    • v.8 no.1
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    • pp.129-136
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    • 2001
  • We introduce and discuss a new numerical method for solving system of second order boundary value problems, where the solution is required to satisfy some extra continuity conditions on the subintervals in addition to the usual boundary conditions. We show that the present method gives approximations which are better than that produced by other collocation, finite difference and spline methods. Numerical example is presented to illustrate the applicability of the new method. AMS Mathematics Subject Classification : 65L12, 49J40.

ALGORITHMS FOR NONLINEAR MIXED VARIATIONAL INEQUALITIES

  • Muhammad Aslam Noor;Eisa A. Al-Said
    • Journal of applied mathematics & informatics
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    • v.5 no.2
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    • pp.313-328
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    • 1998
  • In this paper we establish the equivalence between the generalized nonlinear mixed variational inequalities and the gener-alized resolvent equations. This equivalence is used to suggest and analyze a number of iterative algorithms for solving generalized vari-ational inequalities. We also discuss the convergence analysis of the propose algorithms. As special cases we obtain various known re-sults from our results.

CUBIC SPLINE METHOD FOR SOLVING TWO-POINT BOUNDARY-VALUE PROBLEMS

  • Al Said, Eisa-A.
    • Journal of applied mathematics & informatics
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    • v.5 no.3
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    • pp.759-770
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    • 1998
  • In this paper we use uniform cubic spline polynomials to derive some new consistency relations. These relations are then used to develop a numerical method for computing smooth approxi-mations to the solution and its first second as well as third derivatives for a second order boundary value problem. The proesent method out-performs other collocations finite-difference and splines methods of the same order. numerical illustratiosn are provided to demonstrate the practical use of our method.

QoS Implementation on a Clustering Web Server (클러스트링 웹서버에서의 QoS 구현에 관한 연구)

  • Park, Jong-Gyu;Lee, Do-Young;Chang, Whie;Kim, Hag-Bae
    • Proceedings of the KIEE Conference
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    • 2000.07d
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    • pp.2980-2982
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    • 2000
  • 본 연구에서는 리눅스 기반의 클러스터링 웹서버를 구성하고, 이 클러스터링 웹서버를 하나의 서버인 것처럼 관리하는 개발툴을 만들었다. 그리고 커널 패치를 통하여 로드밸런서가 다양한 시스템 정보를 밸런싱에 이용하도록 하였다. 각 리얼서버에는 응답 데이터의 양 혹은 각 패킷의 길이에 따라 전송의 순서를 결정하는 QoS를 구현하였다.

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Synthesis of certain N-Aryl-N'-(2-pyrimidinyl)guanidine derivatives as potential antimicrobial agents

  • Eisa, H.M.;Tayel, M.A.;Yousif, M.Y.;El-Kerdway, M.M.
    • Archives of Pharmacal Research
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    • v.13 no.1
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    • pp.78-81
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    • 1990
  • N-Aryl-N'(4-hydroxy-6-methyl-2-pyrimidinyl)guanidines (IIa-c) were prepared by cyclization of N-arybiguanides (Ia-c) with ethyl acetoacetate. Coupling of compounds (IIa-c) with the appropriate diazotized arylamine gave N-aryl-N'-(5-arylhydrazono-6-methyl-4-oxopyrimidin-2-yl) guanidines (IIIa-f). Whereas, their chlorination with phosphorus oxychloride followed by treatment of N-aryl-N'-(4-Chloro-6-methyl-2-pyrimidinyl)guanidimes (IVac) with the appropriate arylamine afforded the corresponding 4-arylamino derivatives (Vaf). Compounds (IIa-f) were also formed when compounds (1a-c) were treated with ethyl 2-arylhydrazono-3-oxobutyrates. The antimircobial testing of some of the prepared compounds against some pathogenic microorganisms revealed that only two have a marked effect against Escherichia coli.

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Synthesis of certain 2-aminoadmantane derivatives as potential antimicrobial agents

  • Eisa, Hassan M.;Tantawy, Atif S.;El-Kerdawy, Mohamed-M.
    • Archives of Pharmacal Research
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    • v.13 no.1
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    • pp.74-77
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    • 1990
  • N-(2-Amamantyl)-N-(5-arylhydrazono-6-methyl-4-oxopyrimidin-2-yl) guanidines (IIIa, b), 2-(2-admantyl-amino)-4-amino-s-triazine (IVa) and its 6-chloromethyl derivative (IVb) were prepared by cylization of 1-(2-admantyl) biguanide HCl (I) with ethyl 2-arylhydrazono-3-oxobutyrates (II), ethyl formate and ethyl chloroacetate, respectively. Where 1-(2-admantyl)-3-(4, 5-dioxo-2-imidazolidinylidene)guanidine (V) was used as intermediate for the synthesis of amides (VIIa, b), hydrazide (VIII) and azomethine derivatives (IX, b) of alkyl 2-(2-admantyl-amino)-4-amino-2-triazine-6-carboxylates (VI a, b). The antimicrobial testing of the prepared compounds proved that compound 1Xb was the most active. It showed a marked bacteriostatic effect against Staphylococcus aureus and Bacillus subtilis.

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