• Title/Summary/Keyword: EB technology

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Fluorescence Enhancement of Ethidium Bromide by DNA Bases and Nucleosides

  • Pyun, Chong-Hong;Park, Su-Moon
    • Bulletin of the Korean Chemical Society
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    • v.10 no.2
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    • pp.142-147
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    • 1989
  • Fluorescence enhancements of ethidium bromide (EB) by solution species of low molecular weights such as DNA base molecules and nucleosides in water are reported. The degree of enhancements was determined by intensity as well as lifetime measurements for EB fluorescence. Experiments including solvent effects on absorbance and fluorescence spectra of EB, effects of protonation on the EB absorbance spectrum, and determination of equilibrium constants for EB-DNA bases have been performed to help explain the fluorescence enhancement. The results suggest that the excited state stabilization in the hydrophobic environment, the loss of torsional/vibrational energy of amino groups, and the change in the electronic transition characteristics are all responsible for the fluorescence enhancement.

Removal of EDCs from Industrial Sludge by Electron Beam

  • Han, Bumsoo;Kim, Jinkyu;Kim, Yuri;Jung, Seungtae;Park, Junhyung;Choi, Jangseung
    • Journal of Radiation Industry
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    • v.8 no.2
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    • pp.71-76
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    • 2014
  • Endocrine disrupting chemicals (EDCs) and potential EDCs are mostly man-made, found in various materials such as pesticides, additives or contaminants in food, and personal care products. The high energy ionizing radiation has the ability to remove the EDCs with a very high degree of reliability and in a clean and efficient manner. The ionizing radiation interacts with EDCs both directly and indirectly. Direct interaction takes place with EDCs and the structure of EDCs is destroyed or changed. During indirect interaction, radiolysis products of water result in the formation of highly reactive intermediates which then react with the target molecules, culminating in structural changes. To confirm the radiation reduction of EDCs in industrial sludge, a pilot scale experiment up to 50 kGy of electron beam (EB) was conducted with samples from the textile dyeing industries. The experimental result showed the over 90% of reduction of Nonylphenol (NP) and Di(2-ethylhexyl) phthalate (DEHP) at around 10 kGy of absorbed doses.

Strain interaction of steel stirrup and EB-FRP web strip in shear-strengthened semi-deep concrete beams

  • Javad Mokari Rahmdel;Erfan Shafei
    • Steel and Composite Structures
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    • v.47 no.3
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    • pp.383-393
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    • 2023
  • Conventional reinforced concrete design codes assume ideal strain evolution in semi-deep beams with externally bonded fiber-reinforced polymer (EB-FRP) web strips. However, there is a strain interaction between internal stirrups and web strips, leading to a notable difference between code-based and experimental shear strengths. Current study provides an experiment-verified detailed numerical framework to assess the potential strain interaction under quasi-static monotonic load. Based on the observations, steel stirrups are effective only for low EB-FRP amounts and the over-strengthening of semi-deep beams prevents the stirrups from yielding, reducing its shear strength contribution. A notable difference is detected between the code-based and the study-based EB-FRP strain values, which is a function of the normalized FRP stress parameter. Semi-analytical relations are proposed to estimate the effective strain and stress of the components considering the potential strain interaction. For the sake of simplification, a linearized correction factor is proposed for the EB-FRP web strip strain, assuming its restraining effect as constant for all steel stirrup amounts.

Isolation and characterization of an antifungal substance from Burkholderia cepacia, an endophytic bacteria obtained from roots of cucumber.

  • Park, J.H.;Park, G.J.;Lee, S.W;Jang, K.S.;Park, Y.H.;Chung, Y.R.;Cho, K.Y.;Kim, J.C.
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.95.2-96
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    • 2003
  • In order to develop a new microbial fungicide for the control of vegetable diseases using endophytic bacteria, a total of 260 bacterial strains were isolated from fresh tissues of 5 plant species. After they were cultured in broth media, their antifungal activities were screened by in vivo bioassays against Botrytis cinerea(tomato gray mold), Pythium ultimum(cucumber damping-off), Phytopkhora infestans(tomato late blight), Colletotrichum orbiculare(cucumber anthracnose), and Blumeria graminis f. sp. hordei(barley powdery mildew). As the results of screening, 38 bacterial strains showed potent antifungal activities against at least one of 5 plant pathogens. A bacterial strain EB072 displayed potent disease control activities against 3 plant diseases. Among the bacterial strains with a potent antifungal activity against cucunlber anthracnose, three bacterial strains, EB054, EB151 and EB215, also displayed a potent in vitro antifungal activity against C. acutatum, a fungal agent causing pepper anthracnose. A bacterial strain EB215 obtained from roots of cucumber was identified as Burkholderia cepacia based on its physiological and biochemical characteristics and 165 rRNA gene sequence. An antifungal substance was isolated from the liquid cultures of B. cepacia EB215 strain by ethyl acetate partitioning, repeated silica gel column chromatography, and invitro bioassay, Its structural determination is in progress by various instrumental analyses.

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Inhibition of Food-derived Lactic Acid Bacterial Biofilm Formation Using Eisenia bicyclis-derived Nanoparticles (식품 유래 Biofilm 형성 유산균에 대한 대황(Eisenia bicyclis) 유래 Nanoparticle의 Biofilm 형성 저해)

  • Do Kyung Oh;Fazlurrahman Khan;Seul-Ki Park;Du-Min Jo;Kyung-Jin Cho;Geum-Jae Jeong;Yeon-Ju Sim;Jeong Mi Choi;Jae-Ho Woon;Young-Mog Kim
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.57 no.2
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    • pp.129-136
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    • 2024
  • Lactic acid bacteria (LAB) growth in processed meat products produces slime. In this study, 10 different biofilm-forming LAB, including Leuconostoc mesenteroides, Lacticaseibacillus paracasei, Levilactobacillus brevis, Lactiplantibacillus plantarum, Leuconostoc citreum, Weissella viridescens, and Latilactobacillus sakei, were isolated from various meat products and identified based on 16S rRNA gene analysis. To inhibit biofilm formation by LABs, Eisenia bicycles methanolic extract (EB) and ethyl acetate soluble fraction (EA) were used as antibacterial and antibiofilm agents, respectively. Furthermore, EA and EB were employed to synthesize gold nanoparticles (AuNPs) such as EB-AuNPs and EA-AuNPs, which could serve as antibiofilm agents against the isolated LAB. These findings demonstrate that EA, EB-AuNPs, and EA-AuNPs exhibit significant antibacterial activity against the isolated LAB. Furthermore, EB-AuNPs reduced L. citreum biofilm production, whereas EA-AuNPs inhibited L. mesenteroides and L. brevis biofilm formation. The current results suggest that EB-AuNPs and EA-AuNPs can be used as nanomaterials to inhibit LAB that form biofilms on meat products.

End to End Business Process Management System using ebXML and Web service

  • Choi S.W.;Hwang J.G.
    • Proceedings of the KSRS Conference
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    • 2004.10a
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    • pp.355-357
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    • 2004
  • With the introduction to ebXML and web service related standards, enterprises struggle to adapt to the rapidly evolving technology to meet the complex needs of the enterprise customer. The ability to integrate and interoperate individual services within enterprise and with other enterprise's information technology infrastructure using standard-based business processes is an important element of business process management system. For over 25 years EDI has established VAN based solution of exchanging business information in electronic form. However EDI solutions are only accessible to large organizations due to the cost factor. Moreover lack of well accepted B2B business process standards is hindering the success of promoting interoperability between organizations of any size. ebXML work is focused on defining the standard B2B business process context and its runtime semantics in order to remove these hindrances. However ebXML framework does not recommend any particular implementation model to interact with enterprise internal system. This paper propose a end to end business process management architecture by applying ebXML in the front end of the enterprise system and using BPEL to integrate front end services into related services within enterprise.

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GRAIN SIZE AND TOUGHNESS OF TI-6AL-4V ELECTRON BEAM AND TIG WELD DEPOSITS

  • Kivineva, Esa;Hannerz, Niis-Erik
    • Proceedings of the KWS Conference
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    • 2002.10a
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    • pp.632-638
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    • 2002
  • Electron beam (EB) and Gas tungsten arc (TIG) welds were performed on 12.7 mm thick Ti-6Al-4V plate (ASTM Titanium Grade 5). Charpy-V toughness and hardness, as well as, microstructure of the welds and penetration from the macrostructure were studied. It appears that by EB welding rather smaller $\beta$-grains than with TIG welding can be obtained. Next to the fusion line the $\beta$-grain size in the HAZ was 50 ${\mu}{\textrm}{m}$l while in the weld metal it was 150 ${\mu}{\textrm}{m}$. Charpy-V toughness of the EB weld metal was equal or even better to that of base metal, which shows that the $\alpha$-martensite per se is not particularly brittle if only the grain size is fine enough. This is similar to behavior of low carbon martensite in steel. The grain size was studied with light optical and scanning electron (SEM) microscopes. Thus for products, for products which can be manufactured automatically with very narrow fit, the EB welding of Ti-6Al-4V appears to yield satisfactory toughness without any complex post weld heat treatment. ill this study as in earlier studies the TIG welds gave lower toughness than that of the base material due to the higher heat input and slower cooling as compared to EB welding.

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The Effect of the Types of Learning Material and Epistemological Beliefs in an Ill-structured Problem Solving

  • OH, Suna;KIM, Yeonsoon;KANG, Sungkwan
    • Educational Technology International
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    • v.16 no.2
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    • pp.183-200
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    • 2015
  • This study investigated the effect of learning achievements and cognitive load according to different types of presenting learning materials and epistemological beliefs (EB). Learning achievements in this study were composed by retention and transfer of ill-structured problem. A total of 80 college students participated in the study. Prior to the learning, students were guided to fill out a questionnaire regarding epistemological beliefs and a prior knowledge test. The students of each group studied with a different type of reading material: full text (FT), full text including key questions (KeyFT) and full text including a concept map (CmFT). After a session of study was finished, they were asked to complete the posttest: retention and transfer. The results showed that there was a significant difference in transfer achievements. CmFT outperformed higher scores than the other types. There was no significant difference in retention among the groups. It is strongly believed that the types of presenting learning materials may have affected the understanding of ill-structured problem solving skills. Students with sophisticated EB showed higher achievements on retention and transfer than naive-EB and mixed-EB. Even though the data showed decrease of the cognitive load on the type of materials and EB, there were no significant differences on the cognitive load. We should consider a positive effect of types of presenting learning materials and EB enhancing capabilities of solving ill-structured problems in real life.

A Study on the Thermal Shock Resistance of Sintered Zirconia for Electron Beam Deposition (전자빔 증착을 위한 소결체 지르코니아의 열충격 저항성 연구)

  • Oh, Yoonsuk;Han, Yoonsoo;Chae, Jungmin;Kim, Seongwon;Lee, Sungmin;Kim, Hyungtae;Ahn, Jongkee;Kim, Taehyung;Kim, Donghoon
    • Journal of the Korean Society of Propulsion Engineers
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    • v.19 no.3
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    • pp.83-88
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    • 2015
  • Coating materials used in the electron beam (EB) deposition method, which is being studied as one of the fabrication methods of thermal barrier coating, are exposed to high power electron beam at focused area during the EB deposition. Therefore the coating source for EB process is needed to form as ingot with appropriate density and microstructure to sustain their shape and stable melts status during EB deposition. In this study, we tried to find the optimum powder condition for fabrication of ingot of 8 wt% yttria stabilized zirconia which can be used for EB irradiation. It seems that the ingot, which is fabricated through bi-modal type initial powder mixture which consists of tens of micro and nano size particles, was shown better performance than the ingot which is fabricated using monolithic nanoscale powder when exposed to high power EB.

Characterization of the 2.5 MeV ELV electron accelerator electron source angular distribution using 3-D dose measurement and Monte Carlo simulations

  • Chang M. Kang;Seung-Tae Jung;Seong-Hwan Pyo;Youjung Seo;Won-Gu Kang;Jin-Kyu Kim;Young-Chang Nho;Jong-Seok Park;Jae-Hak Choi
    • Nuclear Engineering and Technology
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    • v.55 no.12
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    • pp.4678-4684
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    • 2023
  • Using the Monte Carlo method, the impact of the angular distribution of the electron source on the dose distribution for the 2.5 MeV ELV electron accelerator was explored. The experiment measured the 3-D dose distribution in the irradiation chamber for electron energies of 1.0 MeV and 2.5 MeV. The simulation used the MCNP6.2 code to evaluate three angular distribution models of the source: a mono-directional beam, a cone shape, and a triangular shape. Of the three models, the triangular shape with angles θ = 30°, φ = 0° best represents the angle of the scan hood through which the electron beam exits. The MCNP6.2 simulation results demonstrated that the triangular model is the most accurate representation of the angular distribution of the electron source for the 2.5 MeV ELV electron accelerator.