• Animal Bioscience
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• v.35 no.2
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• pp.260-271
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• 2022

Objective: We aimed to investigate the effect of the differing amino acid (AA) release dynamics of two protein sources on the growth performance, nitrogen deposition, plasma biochemical parameters, and muscle synthesis and degradation of piglets when included in their diets at normal and low concentrations. Methods: Forty-eight piglets (Duroc×Landrace×Large White) with initial body weight of 7.45±0.58 kg were assigned to six groups and fed one of 6 diets. The 6 dietary treatments were arranged by 3×2 factorial with 3 protein sources and 2 dietary protein levels. They are NCAS (a normal protein content with casein), NBlend (a normal protein content with blend of casein and corn gluten meal), NCGM (a normal protein content with corn gluten meal), LCAS (a low protein content with casein), LBlend (a low protein content with blend of casein and corn gluten meal), LCGM (a low protein content with corn gluten meal). The release dynamics of AA in these diets were determined by in vitro digestion. The digestibility, utilization and biological value of nitrogen in piglets were determined by micro Kjeldahl method. Plasma insulin was measured by enzyme-linked immunosorbent assay kits. The protein expression of mediators of muscle synthesis and degradation was determined by western blotting. Results: Although the consumption of a low-protein diet supplemented with crystalline AA was associated with greater nitrogen digestion and utilization (p<0.05), the final body weight, growth performance, nitrogen deposition, and phosphorylation of ribosomal protein S6 kinase 1 and eIF4E binding protein 1 in the muscle of pigs in the low-protein diet-fed groups were lower than those of the normal-protein diet-fed groups (p<0.05) because of the absence of non-essential AA. Because of the more balanced release of AA, the casein (CAS) and Blend-fed groups showed superior growth performance, final body weight and nitrogen deposition, and lower expression of muscle ring finger 1 and muscle atrophy F-box than the CGM-fed groups (p<0.05). Conclusion: We conclude that the balanced release of AA from CAS containing diets and mixed diets could reduce muscle degradation, favor nitrogen retention, % intake and improve growth performance in pigs consuming either a normal- or low-protein diet.

• International Journal of Fluid Machinery and Systems
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• v.5 no.3
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• pp.100-108
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• 2012

Design parameters for suction channels of process centrifugal compressors were investigated, and an optimization method to enhance stage efficiency by using the new design parameters was proposed. From results of computational fluid dynamics, the passage sectional area ratios $A_c/A_e$, $A_e/A_s$ and $A_c/A_s$ were found to be the dominant parameters for the pressure loss and circumferential flow distortion, where $A_c$, $A_e$ and $A_s$ are passage sectional areas for the casing upstream side, casing entrance and impeller eye, respectively. The Base suction channel was optimized using the new design parameters, and the Base and Optimized types were tested. Test results showed that the Optimized suction channel achieved 3.8% higher stage efficiency than the Base suction channel while maintaining the same operating range.

• Journal of Life Science
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• v.33 no.11
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• pp.851-858
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• 2023

Unique cartilage matrix-associated protein (UCMA) is an extrahepatic vitamin K-dependent protein rich in γ-carboxylated (Gla) residues. UCMA has been recognized for its ability to promote osteoblast differentiation and enhance bone formation; however, its impact on osteoblasts under hyperglycemic stress remains unknown. In this paper, we investigated the effect of UCMA on MC3T3-E1 osteoblastic cells under hyperglycemic conditions. After exposure to high glucose, the MC3T3-E1 cells were treated with recombinant UCMA proteins. CellROX and MitoSOX staining showed that the production of reactive oxygen species (ROS), which initially increased under high-glucose conditions in MC3T3-E1 cells, decreased after UCMA treatment. Additionally, quantitative polymerase chain reaction revealed increased expression of antioxidant genes, nuclear factor erythroid 2-related factor 2 and superoxide dismutase 1, in the MC3T3-E1 cells exposed to both high glucose and UCMA. UCMA treatment downregulated the expression of heme oxygenase-1, which reduced its translocation from the cytosol to the nucleus. Moreover, the expression of dynamin-related protein 1, a mitochondrial fission marker, was upregulated, and AKT signaling was inhibited after UCMA treatment. Overall, UCMA appears to mitigate ROS production, increase antioxidant gene expression, impact mitochondrial dynamics, and modulate AKT signaling in osteoblasts exposed to high-glucose conditions. This study advances our understanding of the cellular mechanism of UCMA and suggests its potential use as a novel therapeutic agent for bone complications related to metabolic disorders.

• Korean Journal of Ichthyology
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• v.9 no.1
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• pp.141-152
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• 1997

The dynamics of fish community was investigated in the Lake Paldang from September 1995 to August 1996. The collected fishes were classified into forty two species involving in eleven families. Dominant species was Erythroculter erythropterus (relative abundance : 27.96%) and subdominant species was Lepomis macrochirus (20.69%). Also, Squalidus japonicus coreanus (19.25%), Opsariichthys bidens (4.07%), Hemibarbus labeo (3.42%), Acheilognathus yamatsutae (2.68%) and Micropterus salmoides (2.68%) were numerous. Dominant species was L. macrochirus in spring and autumn and S. japonacus coreanus in summer. E. erythropterus was subdominated in all seasons. The still-water fishes increased after construction of Paldang Dam. Ten were endemic species in Korea. Four species were exotic, and they occupied 24.75% in relative abundance. Dominance index was high at St. 2, diversity and evenness at St. 5, and richness at St. 4. In these stations, collection was made with casting net and scoop net. In the stations where collection was made with fixed shore net, dominance index was high at St. 8, diversity and evenness at St. 14, and richness at St. 7. E. erythropterus showed a stable age structure, while L. macrochirus was composed of mainly 3~4 year classes.

• International Journal of Naval Architecture and Ocean Engineering
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• v.2 no.3
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• pp.139-145
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• 2010

In the present study, we conducted resistance test, propeller open water test and self-propulsion test for a ship's resistance and propulsion performance, using computational fluid dynamics techniques, where a Reynolds-averaged Navier-Stokes equations solver was employed. For convenience of mesh generation, unstructured meshes were used in the bow and stern region of a ship, where the hull shape is formed of delicate curved surfaces. On the other hand, structured meshes were generated for the middle part of the hull and the rest of the domain, i.e., the region of relatively simple geometry. To facilitate the rotating propeller for propeller open water test and self-propulsion test, a sliding mesh technique was adopted. Free-surface effects were included by employing the volume of fluid method for multi-phase flows. The computational results were validated by comparing with the existing experimental data.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.18 no.3
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• pp.363-372
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• 2020

Anionic radionuclides pose one of the highest risks to the long-term safety assessments of disposal repositories. Therefore, techniques to immobilize and separate such anionic radionuclides are of crucial importance from the viewpoints of safety and waste volume reduction. The main objective of this study is to design a separator with minimum pressure disturbance, based on the concept of a conventional cyclone separator. We hypothesize that the anionic radionuclides can be immobilized onto a nanomaterial-based substrate and that the particles generated in the process can flow via water. These particles are denser than water; hence, they can be trapped within the cyclone-type separator because of its design. We conducted particle tracking analysis using computational fluid dynamics (CFD) for the conventional cyclone separator and studied the effects due to the morphology of the separator. The proposed sandglass-like design of the separator shows promising results (i.e., only one out of 10,000 particles escaped to the outlet from the separation zone). To validate the design, we manufactured a laboratory-scale prototype separator and tested it for iron particles; the efficiency was ca. 99%. Furthermore, using an additional magnetic effect with the separator, we could effectively separate particles with ~100% efficiency. The proposed sandglass-like separator can thus be used for effective separation and recovery of immobilized anionic radionuclides.

• Journal of the Korean Society of Marine Environment & Safety
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• v.28 no.3
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• pp.441-450
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• 2022

The introduction of autonomous underwater gliders (AUGs) specifically addresses the reduction of operational costs that were previously prohibited with conventional autonomous underwater vehicles (AUVs) using a "scaling-down" design philosophy by utilizing the characteristics of autonomous drifters to far extend operation duration and coverage. Long-duration, wide-area missions raise the cost and complexity of in-water testing for novel approaches to autonomous mission planning. As a result, a simulator that supports the rapid design, development, and testing of autonomy solutions across a wide range using software-in-the-loop simulation at faster-than-real-time speeds becomes critical. This paper describes a faster-than-real-time AUG simulator that can support high-resolution bathymetry for a wide variety of ocean environments, including ocean currents, various sensors, and vehicle dynamics. On top of the de facto standard ROS-Gazebo framework and open-sourced underwater vehicle simulation packages, features specific to AUGs for ocean mapping are developed. For vehicle dynamics, the next-generation hybrid autonomous underwater gliders (Hybrid-AUGs) operate with both the buoyancy engine and the thrusters to improve navigation for bathymetry mappings, e.g., line trajectory, are is implemented since because it can also describe conventional AUGs without the thrusters. The simulation results are validated with experiments while operating at 120 times faster than the real-time.

• Journal of the Mineralogical Society of Korea
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• v.29 no.4
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• pp.209-220
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• 2016

Clay minerals are a major player to determine geochemical cycles of trace metals and carbon in the critical zone which covers the atmosphere down to groundwater aquifers. Molecular dynamics (MD) simulations can examine the Earth materials at an atomic level and, therefore, provide detailed fundamental-level insights related to physicochemical properties of clay minerals. In the current study, we have applied classical MD simulations with clayFF force field to dioctahedral clay minerals (i.e., gibbsite, kaolinite, and pyrophyllite) to analyze and compare structural parameters (lattice parameter, atomic pair distance) with experiments. We further calculated vibrational power spectra for the hydroxyls of the minerals by using the MD simulations results. The MD simulations predicted lattice parameters and interatomic distances respectively deviated less than 0.1~3.7% and 5% from the experimental results. The stretching vibrational wavenumber of the hydroxyl groups were calculated $200-300cm^{-1}$ higher than experiment. However, the trends in the frequencies among different surface hydroxyl groups of each mineral was consistent with experimental results. The angle formed by the surface hydroxyl group with the (001) plane and hydrogen bond distances of the surface hydroxyls were consistent with experimental result trends. The inner hydroxyls, however, showed results somewhat deviated from reported data in the literature. These results indicate that molecular dynamics simulations with clayFF can be a useful method in elucidating the roles of surface hydroxyl groups in the adsorption of metal ions to clay minerals.

• Journal of the Korean Solar Energy Society
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• v.27 no.3
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• pp.117-125
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• 2007

This study proposes a CFD(Computational Fluid Dynamics) analysis as a method of verification of the designed-data and a supplement of the insufficient experiences in geothermal system, which shows a rapid growth among the renewable energies. The followings are the results. FLUENT 6.2.12 is used as a CFD tool on this study, with the equations of continuity, motion, energy for unsteady flow through pipes and k-epsilon turbulent model. S-type model which has one borehole with diameter 12m by depth 206m and T-type model which has 3 boreholes with $12m{\times}20m{\times}206m$ are proposed, and also the boundary conditions are described. The temperature differences between temperatures by CFD analysis and by on-site measurement are less than 1.5%, this shows a high reliability of CFD analysis process which this study proposes. After 11 days simulation operated 12 hours interval On/Off mode, it is clearly predicted that the outlet temperatures of geothermal pipes are increased by $1.2^{\circ}C$, and $2.2^{\circ}C$ after 4 months. And the outlet temperatures of geothermal pipes increased with increase of the mass flow rates through the pipes. T-type model shows that the 4m distance between boreholes are reasonable because the temperatures at 2m and 6m from boreholes are nearly same.

• Journal of Soil and Groundwater Environment
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• v.17 no.6
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• pp.23-32
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• 2012

This study was conducted to monitor microbial species dynamics within the aquifer due to long term operation of geothermal heat pump system. The species were identified by molecular biological methods of 16S rDNA. Groundwater sample was collected from both open (S region) and closed geothermal recovery system (J region) along with the control. J measured and control as well as S measured found Ralstonia pickettii as dominant species at year 2010. In contrast, Rhodoferax ferrireducens was dominantly observed for the control of S. In 2011, Sediminibacterium sp. was universely identified as the dominant species regardless of the monitoring places and type of sample, i.e., measured or control. The difference in the dynamics between the measured and the control was not critically observed, but annual variation was more strikingly found. It reveals that possible environmental changes (e.g. ORP and DO) due to the operation of geothermal heat recovery system in aquifer could be more exceedingly preceded to differentiate annual variation of microbial species rather than positional differences.