• Title/Summary/Keyword: E2HE2H

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Field Emission Stability of Carbon Nanotubes Grown by Thermal Chemical Vapor Deposition

  • Kim, B.K.;Kong, B.Y.;Seon, J.Y.;Lee, N.S.;Kim, H.J.;Han, I.T.;Choi, J.H.;Jung, J.E.;Kim, J.M.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2003.07a
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    • pp.863-866
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    • 2003
  • Multi-walled carbon nanotubes (CNTs) were synthesized on glass substrates in the different ramp-up heating ambient of vacuum, He, Ar, and $N_{2}$ by thermal chemical vapor deposition. CNTs with higher crystallinity were developed in the buffer gases with faster growth rates than in vacuum. Field emission characteristics were strongly related to the relative position of CNT emitters to the cathode electrodes. The areal-spread emission and instability were overcome by locating the emitters far away from the edges of cathode electrodes. The electrical conditioning of emitters improved their emission uniformity over a large area although it decreased the emission current. This study also discussed the long-term stability of CNT emitters.

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Hypothermia Effect on Apoptotic Neuronal Death in Traumatic Brain Injury Model

  • Yoo, Do-Sung;Lee, Soon-Kyu;Huh, Pil-Woo;Han, Young-Min;Rha, Hyung-Kyun;Kim, Dal-Soo
    • Journal of Korean Neurosurgical Society
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    • v.38 no.3
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    • pp.215-220
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    • 2005
  • Objective : Many researchers believe that the hypothermia shows neuro-protective effect on brain injury. To understand the molecular mechanism of the hypothermic treatment, this study investigated its effects on the expression of cell death or survival related proteins such as p53, Bcl-2 and Bax in the rat traumatic brain injury[TBI] model. Methods : Twenty rats [Spraque Dawley, $200{\sim}250g$] were subjected to the brain injury of moderate severity [$2.4{\sim}2.6atm$] using the fluid percussion injury device and five rats were received only same surgery as controls. During 30minutes after the brain injury, the hypothermia group was maintained the body temperature around $34^{\circ}C$ while the control group were maintained that of $36^{\circ}C$. Five rats in each group were sacrificed 12h or 24h after brain injury and their brain sections was analyzed for physical damages by H-E stains and the extent of apoptosis by TUNEL assay and immunohistochemical stains. The tissue damage after TBI was mainly observed in the ipsilateral cortex and partly in the hippocampus. Results : Apoptosis was observed by TUNEL assay and the Bax protein was detected in both sample which harvested 12h and 24h after TBI. In the hypothermia treatment group, tissue damage and apoptosis were reduced in HE stains and TUNEL assay. In hypothermia treatment group rat shows more expression of the Bcl-2 protein and shows less expression of the Bax protein, at both 12h and 24h after TBI. Conclusion : These results show that the hypothermia treatment is an effective treatment after TBI, by reducing the apoptotic process. Therefore, it could be suggested that hypothermia has a high therapeutic value for treating tissue damages after TBI.

Effects of Solvent-extracts Extracted from Coptis chinensis on Seed Germination and Seedling Growth (종자발아 및 유묘생장에 미치는 황련(Coptis chinensis) 추출물의 영향)

  • Lee Seung-Yeob;Kweon Tae-Oh;Bae Jong-Hyang
    • Journal of Bio-Environment Control
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    • v.15 no.3
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    • pp.277-282
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    • 2006
  • For searching the natural herbicide-components, the seed germination and seedling growth of receptor plant species (Brassica campestris, Sesamum indicum, Perilla frutescens and Echinochloa crus-galli) were investigated in four solvent-extracts extracted from Coptis chinensis Franch. The seed germination of receptor plant species was largely inhibited in 2,000 ppm of ethyl acetate compared to the control, and it was inhibited in order of P. frutescens, B. campestris, E. crux-galli, and S. indicum. In seedling growth, the shoot and root elongations of receptor plant species were inhibited in order of S. indicum, P. frutescens, B. campostris, and E. crus-galli. Root elongation was remarkably reduced in order of $H_2O$, butyl alcohol, and hexane, ethyl acetate extracts. Of four receptor plant species, seed germination and seedling growth of B. campestris and S. indicum showed the species-specific reaction to the solvent-extracts extracted from C. chinemis. $H_2O$ extract had a natural herbicide potential to the seed germination or root elongation in B. campestris and S. indicum. The result can be provided a basic data f3r the development of natural herbicide.

Characteristics of Chromium, Copper, and Arsenic Leaching from CCA-Treated Wood (CCA 방부처리 목재로부터 크롬, 구리 및 비소의 용탈 특성)

  • Kim, He-Kap;Kim, Dong-Jin
    • Environmental Analysis Health and Toxicology
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    • v.22 no.4
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    • pp.339-348
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    • 2007
  • A laboratory experiment was conducted to study the characteristics of leaching of Cr, Cu, and As from chromated topper arsenate (CCA)-treated wood. The wood species tested was hemlock spruce ($10\;cm\;{\times}\;10\;cm\;{\times}\;10\;cm\;tube$). The leaching experiment was conducted over 60 days using I L of leachants whose pHs were adjusted to 2.0, 3.7, and 1.6, respectively with nitric acid, and also using lake water, according to the OECD guideline. Each leachate was analyzed for Cr and Cu using flame-AAS, and for As using vapor generation-AAS. Three metals were loathed at the highest levels at pH 2.0 but almost at similar levels at the other conditions. Cumulative quantifies over 60 days of a leaching period were in order of As>Cu>Cr. As was predicted to leach with an increase in flux over a 10 year period, while Cr and Cu fluxes were predicted to decrease with time. This result suggest that arsenic can pose a health risk to humans over a long period of time, when CCA-treated wood is used for building facilities (e.g., playgrounds, residential purposes, etc.) with which humans frequently contact.

Regional Distribution Characteristics of Swans(Cygnus spp.) in the Nakdong River Downstream from October 2008 to September 2013, Busan, R. O. Korea (낙동강하류에서 고니류(Cygnus spp.)의 지역별 분포 특성)

  • Soon-Bok Hong;Ji-Pyo Hong
    • Journal of Environmental Science International
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    • v.32 no.7
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    • pp.493-502
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    • 2023
  • This study was conducted to understand the regional distribution characteristics of swans(Cygnus spp.) in downstream of t he Nakdong River , R.O.Korea from October 2008 to September 2013. During this period, a total of two species and 37,518 ind ividuals of Swans(Cygnus spp.) were observed, including 31,596 Whooper Swans(Cygnus cygnus) and 5,922 Tundra Swans (Cygnus columbianus), respectively. The average number of individuals observed in fifteen different areas was 2255.33 in D aemadeung(A), 143.50 in Jangja·Sinjado(B), 304.00 in Sajado·Doyodeung(C), 1928.00 in Lower Ulsukdo(D), 1392.67 in Ulsu kdo(E), 50.17 in Ilwoongdo(F), 91.17 in Yeommak(G), 5.17 in Maekdogang(H), 0.00 in Pyeonggangcheon(I), 0.00 in Lower Noksan sluice(J), 2.83 in Upper Noksan sluice(K), 6.17 in Jomangang·Doonchido(L), 4.50 in Chideung(M), 0.83 in Joongsado (N)and 66.17 in Daejeo sluice(O). The total average of these fifteen areas was 480.81. There was a significant difference am ong the survey areas (Kruskal-Wallis test, 𝒳2=4055.68, P<0.001). In particular, the observed numbers were larger in Dae madeung, Lower Ulsukdo and Ulsukdo than in the other regions.

Etude sur le v tement dans le Proche-Orient (근동지역의 복식 연구(I) -성서에서의 복식의 상징성과 고대 근동지역의 복식 연구-)

  • 오춘자
    • Journal of the Korean Society of Costume
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    • v.19
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    • pp.175-194
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    • 1992
  • Le Proche-Orient est le pays de la Bible, Le peuple He breu ancien, appartenant la race des S mits s mi-nomades, est venu avec Abraham, de m sopotamie en Palestine, terre de Canaan. Nous avons consid r les v tements du peuple des anciens H breux en nous basant sur la Bible et en observant des peintures murales et des bas-reliefs des divers payes de l'ancienne poque, Pour comprendre l' volution des v tements des gens de la pr sente r gion palestinienne, nous avons tudi les v tements traditionnels de la race b douine qui m nent jusqu' maintenant une vie nomade dans le m nent jusqu' maintenant une vie nomade dans le d sert, parce que nous n'avons pas pu connaitre le processus d' volution des v tements apr s l'ancienne poque la suite d' v nements historiques compliques. En conclusion, nous pouvons nous r sumer comme suit: 1) Nous pensons que la kimlah, costume important du peuple h breu ancien est devenu abajeh, manteau des B douins, puisque ces v tements servent prot ger le corps lors des changments de temps, et la nuit on les utilise comme couverture et comme sac pour y mettre des objets, et comme tente lors su travail. en un mot, les fonctions de la Simlah et celles de l'abajeh sont les memes l' poque ancienne et maintenant. 2) Nous pensons que la forme et l'utilisation de la Kethoneth ayant une forme de tunique et de la thob des B douins sont presque semblable. La kethoneth et la thob sont la tuniqu importante que portent jusqu' maintenant tous les peuples du Proche-Orient. 3) Comme on le voit dans la Bible, les femmes du peuple h breu et celles des B douins utilisent le voile pour couvrir la e te, et se servent d'accessoires pour d corer leur corps. A l'avenir, les vVtements des Palestiniens, dans une recherche pous approfondie, feront l'objet de a 2 me partie de l' tude des costumes du Proche-Orient.

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Detection of the expression of a Bombyx mori Atypical Protein Kinase C in BmPLV-Infected Larval Midgut

  • Cao, Jian;He, Yuanqing;Li, Guohui;Chen, Keping;Kong, Jie;Wang, Fenghua;Shi, Jing;Yao, Qin
    • International Journal of Industrial Entomology and Biomaterials
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    • v.22 no.2
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    • pp.59-64
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    • 2011
  • Protein kinase C (PKC) is involved in many cellular signaling pathways, it participates in many physiological processes, such as cell cycle, growth, proliferation, differentiation and apoptosis. To investigate the effect of PKC on the silkworm midgut tissue infection of Bombyx mori parvo-like virus (BmPLV), a B. mori atypical protein kinase C (BmaPKC) gene was cloned from larval midgut tissue, expressed in E. coli and purified. Additionally, the BmPLV susceptible silkworm strain and resistant silkworm strain were used to test the effect of the B. mori infection on BmPLV. The result showed that BmaPKC encodes a predicted 586 amino acid protein, which contains a C-terminal kinase domain and an N-terminal regulatory domain. The maximum expression amount of the soluble (His)6-tagged fusion protein was detected after 0.8 mmol/L IPTG was added and cultured at $21^{\circ}C$. The (His) 6-tagged fusion protein revealed about 73 kDa molecular weight which confirmed by western blot and mass spectrography. Furthermore BmaPKC protein were detected at 0-72 h post-infection in BmPLVinfected larval midgut tissue, western blot showed that as time went on, the expression of BmaPKC increased gradually in susceptible strain, the expression quantity on 72 h is 5 times of 0 h. However, in resistant strain, the expression quantity is slightly lower than susceptible strain. But no significant change in resistant strain was observed as time went on. The available data suggest that BmaPKC may involve in the regulation of BmPLV proliferation.

Leaching and Acute Toxicity Test of Steel-making Slags for Media Contact Recycling (제강슬래그의 매체접촉형 재활용에 따른 중금속 용출특성 및 물벼룩 생태독성 평가)

  • Donghyun Kim;Bong Seok Cho;Won Sik Shin
    • Journal of Soil and Groundwater Environment
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    • v.29 no.1
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    • pp.72-83
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    • 2024
  • Most of the slags generated from steel-making industry in Korea are recycled into media-contact aggregates such as fill and cover materials. For their use as media-contact aggregates, the slags must meet not only the waste quality criteria, but also the Daphnia magna acute toxicity test criteria. In this study, Korean Leaching Test ES 06150.e (Korea), Japanese Leaching Test JIS K 0058-1(Japan), Detuch Leaching Test DIN 19529 (Germany), Toxicity Characteristic Leaching Procedure (USA) were conducted for batch leaching test of slags from 6 Korean steel-making companies. In addition, Korean Standard up-flow percolation test (ES 06151.1) mimicking field conditions was conducted to assess the impact of the slag leachate on the surrounding environment indirectly. Heavy metals such as Cr6+ and Zn2+ were detected from both extractant and leachate samples, but all of them did not exceed waste quality criteria of each country. However, Daphnia magna acute toxicity tests using the leachate samples from up-flow percolation test with slag alone and slag/natural soil conditions exceeded ecotoxicity standard (TU=2) due to their high pH (11.3-12.5). After neutralizing the pH of the slag leachate to 6.5~8.5, the Daphnia magna mortality and immobilization were reduced to satisfy ecotoxicity standard. As the reducing pH of slag leachate would be extremely difficult, appropriate recycling management considering the physicochemical characteristics of he slags should be stuided further.

Immuno-chromatographic Analysis for HPV-16 and 18 E7 Proteins as a Biomarker of Cervical Cancer Caused by Human Papillomavirus

  • Kim, Joo-Ho;Cho, Il-Hoon;Seo, Sung-Min;Kim, Ji-Sook;Oh, Kyu-Ha;Kang, Heun-Soo;Kim, In-Gyu;Paek, Se-Hwan
    • Bulletin of the Korean Chemical Society
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    • v.30 no.12
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    • pp.2999-3005
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    • 2009
  • Among the more than 120 different types of human papillomavirus (HPV), types 16 and 18 have been known to be high risk agents that cause cervical cancer. We examined, in an immuno-chromatographic analysis, the potential of using the early gene product, E7 protein, as a diagnostic marker of cervical cancer caused by HPV. We developed monoclonal antibodies specific to HPV-16 and 18 E7 proteins that were produced from bacterial cells using gene recombinant technology. For each E7 protein, the optimal antibody pair was selected using the immuno-chromatographic sandwichtype binding system based on the lateral flow through membrane pores. Under these conditions, this rapid testing assay had a detection capability as low as 2 ng/mL of E7 protein. Furthermore, since viral analysis required the host cell to be lysed using chemicals such as detergents, it was possible that the E7 protein was structurally damaged during this process, which would result in a decrease in detection sensitivity. Therefore, we examined the detrimental effects caused by different detergents on the E7 protein using HeLa cells as the host. In these experiments, we found that the damage caused by the detergent, nonylphenylpolyethylene glycol (NP-40), was minimal relative to Triton X-100 commonly used for the cell lysis. Temperature also affected the stability of the E7 protein, and we found that the E7 protein was stabilized at 4$^{\circ}C$ for about 2 h, which was 4 times longer than at room temperature. Finally, a HPV-infected cervical cancer cell line, which was used as a real sample model, was treated using the optimized conditions and the presence of E7 proteins were analyzed by immuno-chromatography. The results of this experiment demonstrated that this rapid test could specifically detect HPV-infected samples.

Electron Temperature, Plasma Density and Luminous Efficiency in accordance with Discharge Time in coplanar AC PDPs

  • Jeong, S.H.;Moon, M.W.;Oh, P.Y.;Jeong, J.M.;Ko, B.D.;Park, W.B.;Lee, J.H.;Lim, J.E.;Lee, H.J.;Han, Y.G.;Son, C.G.;Lee, S.B.;Yoo, N.L.;Choi, E.H.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2005.07b
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    • pp.1203-1206
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    • 2005
  • Electron temperature and plasma density in coplanar alternating-current plasma display panels (AC-PDP's) have been experimentally investigated in accordance with discharge time by a micro-probe in this experiment. The resolution of a step mortor to move in micro-Langmuir probe is 10um.[1-3] The used gas in this experiment is He-Ne-Xe (4%) mixure gas. And sustain voltage is 320V which is above of firing voltage for degradation. The electron temperature and plasma density can be obtained from current-voltage (I-V) characteristics of micro Langmuir probe, in which negative to positive bias voltage was applied to the probe. And Efficiency is calculated by formula related discharge power and light emission. Those experiments operated as various discharge time ($0{\sim}72$ Hours). As a result of this experiment, Electron Temperature was increased from 2eV to 5eV after discharge running time of 20 hours and saturates beyond 20 hours. The plasma density is inversely proportional to the square root of electron temperature. So the plasma density was decreased from $1.8{\times}10^{12}cm^{-3}$ to $8{\times}10^{11}cm^{-3}$ at above discharge running time. And the Efficiency was reduced to 70% at 60hours of discharge running time.

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