• Journal of Microbiology and Biotechnology
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• 제31권8호
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• pp.1098-1108
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• 2021

The literature indicates that LINC00174 promotes the growth of colorectal cancer (CRC) cells, but its research needs to be enriched. We tried to explore the function and mechanism of LINC00174 in CRC cell proliferation and migration. Bioinformatics analysis predicted the binding relationship and expressions of lncRNA, miRNA and mRNA. Clinical study analyzes the relationship between LINC00174 and clinical data characteristics of CRC patients. The expressions of LINC00174, miR-3127-5p and E2F7 were verified by RT-qPCR, and the combination of the two was verified by dual luciferase analysis and RNA immunoprecipitation as needed. Western blot was used to detect the expression of EMT-related protein and E2F7 protein. Functional experiments were used to evaluate the function of the target gene on CRC cells. LINC00174 was up-regulated in CRC clinical samples and cells and was related to the clinical characteristics of CRC patients. High-expression of LINC00174, contrary to the effect of siLINC00174, promoted cell viability, proliferation, migration and invasion, up-regulated the expressions of N-Cadherin, Vimentin, E2F7, and inhibited the expression of E-Cadherin. MiR-3127-5p was one of the targeted miRNAs of LINC00174 and was down-regulated in CRC samples. In addition, miR-3127-5p mimic partially reversed the malignant phenotype of CRC cells induced by LINC00174. Besides, E2F7 was a target gene of miR-3127-5p, and LINC00174 repressed miR-3127-5p to regulate E2F7. Our research reveals that LINC00174 affected the biological characteristics of CRC cells through regulated miR-3127-5p/ E2F7 axis.

• Journal of Microbiology and Biotechnology
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• 제34권4호
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• pp.920-929
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• 2024

As a pivotal defensive line against multitudinous malignant tumors, natural killer (NK) cells exist in the tumor microenvironment (TME). RAD18 E3 Ubiquitin Protein Ligase (RAD18) has been reported to foster the malignant progression of multiple cancers, but its effect on NK function has not been mined. Here, the study was designed to mine the mechanism by which RAD18 regulates the killing effect of NK cells on colorectal cancer (CRC) cells. Expression of E2F Transcription Factor 7 (E2F7) and RAD18 in CRC tissues, their correlation, binding sites, and RAD18 enrichment pathway were analyzed by bioinformatics. Expression of E2F7 and RAD18 in cells was assayed by qRT-PCR and western blot. Dual-luciferase assay and chromatin immunoprecipitation (ChIP) assay verified the regulatory relationship between E2F7 and RAD18. CCK-8 assay was utilized to assay cell viability, colony formation assay to detect cell proliferation, lactate dehydrogenase (LDH) test to assay NK cell cytotoxicity, ELISA to assay levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), and immunofluorescence to detect expression of toxic molecules perforin and granzyme B. High expression of RAD18 and E2F7 was found in CRC tissues and cells. Silencing RAD18 could hamper the proliferation of CRC cells, foster viability and cytotoxicity of NK cells, and increase the secretion of GM-CSF, TNF-α, IFN-γ as well as the expression of perforin and granzyme B. Additionally, ChIP and dual-luciferase reporter assay ascertained the binding relationship between RAD18 promoter region and E2F7. E2F7 could activate the transcription of RAD18, and silencing RAD18 reversed the inhibitory effect of E2F7 overexpression on NK cell killing. This work clarified the inhibitory effect of the E2F7/RAD18 axis on NK cell killing in CRC, and proffered a new direction for immunotherapy of CRC in targeted immune microenvironment.

• 대한가정학회지
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• 제28권4호
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• pp.1.1-13
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• 1990

Authors have performed the sensory evaluation tests according to each given items after selecting various lines in order to assess the visual effects by the lines in cloth designing. The evaluations were done by means of ranking tests followed by paired comparison tests. The results obtained were as follows : 1. In the item in than "Shoulder width looks wide", the design C3 showed the best visual effect, and then B1, F8, and A5 comes in order. In "Shoulder width looks narrow", they were A2, F5, F7, and B2 in order. 2. In "Bust looks big", the effect was best in F9, and then B1, F5, C3, and A5 and order. "Bust looks small" item showed A3, C1, and F1 in order. 3. In "Waist looks thick", they were B2, D1, and F7 while in "Waist looks thin", they were B3, F8, and D6 in order. 4. In the item in that "Hip looks big", the best effect was in F9, and then E3, C2, and B4 in order. In "Hip looks small", the best one was C1, and then comes. E1, F6, and F8. 5. In "Upper body looks thick", they were D2, D4, F8, C3 and A5 in order whild in "Upper body looks thin", they were A1, F5, and D7 in order. 6. In the item "Lower body lookds thick", they were F9, C2, E3, B3, and D3 in order. In "Lower body looks thin", the best one was C1, and then D1, E2, F6, and F8 comes in order. 7. In "whole body looks thick", they were F9, F3, D3, and A5, and in "Whole body looks thin", they were F5, A1, C1, and D6 in order. 8. In "Height looks tall", the effects were in order of A4, D6, E1, and F7 while in "Height looks short", they were E3, F9, B4, D2, and D1. 8. In "Height looks tall", the effects were in order of A4, D6, E1, and F7 while in "Height looks short", they were E3, F9, B4, D2, and D1. F9, B4, D2, and D1.

• 멤브레인
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• 제11권4호
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• pp.143-151
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• 2001

고상 반응법을 이용하여 L $a_{0.6}$S $r_{0.4}$ $Co_{0.2}$F $e_{0.8}$ $O_{3-}$$\delta$/ 및 L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ 분말을 합성하고 혼합전도체 분리막을 소결하여 제조하였다. 제조된 분리막들은 정확한 페롭스카이트 결정구조를 나타내었으며, 95% 이상의 높은 상대밀도를 나타내었다. 산소이온 변환 능력을 향상시키기 위해 L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ disk의 양 표면에 L $a_{0.6}$S $r_{0.4}$Co $O_{3-}$$\delta$/ paste를 스크린 프린팅 방법으로 코팅하였으며 코팅 막은 비교적 치밀한 미세구조를 나타내었다. 코팅되지 않은 L $a_{0.6}$S $r_{0.4}$ $Co_{0.2}$F $e_{0.8}$ $O_{3-}$$\delta$/ 및 L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ 분리막과 코팅된 L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ 분리막의 산소투과 성능을 비교 실험한 결과, 90$0^{\circ}C$에서 L $a_{0.6}$S $r_{0.4}$ $Co_{0.2}$F $e_{0.8}$ $O_{3-}$$\delta$/ 분리막이 정상상태에서 0.266 mL/min.$\textrm{cm}^2$로 가장 많은 투과량을 보였으며 코팅된 L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ 분리막의 정상상태 산소 투과 유속은 최고 0.19 mL/min.$\textrm{cm}^2$ 정도로 코팅되지 않은 분리막에 비해 약 2~3배로 높게 나타났다.정도로 코팅되지 않은 분리막에 비해 약 2~3배로 높게 나타났다.코팅되지 않은 분리막에 비해 약 2~3배로 높게 나타났다. 높게 나타났다.

• 한국자기학회지
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• 제2권2호
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• pp.114-118
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• 1992

아크로를 이용하여 제작한 NdF $e_{12-x}$ $Ti_{x}$ (1 .leq. x .leq. 2) 합금의 상분석 결과 이들 합금은 ThM $n_{12}$ 구조의 경자성상(1-12상) 과 반강자성의 F $e_{2}$Ti 및 연자성의 .alpha. -Fe 등으로 구성되며, x=1.3 인 NdF $e_{10.7}$ $Ti_{1.3}$ 조성에서 단상에 가까운 ThM $n_{2}$ 구조가 얻어짐이 밝혀졌다. 한편, 합금중의 1-12 상은 50 .mu. m - 100 .mu. m 의 사각주 형태로 생성되며, FeTi은 1-12상의 결정립계에 고립된 입자의 형태로 존재한다. NdF $e_{10.7}$ $Ti_{1.3}$ 합금을 질화처리할 경우 단위포의 체적, 자화, 이방성자장 및 큐리온도가 증가하며, 이합금을 500 .deg. C 에서 15분간 질화처리한 NdF $e_{10.7}$ $Ti_{1.3}$ $N_{x}$ 의 비포화자화, 이방성자장 및 큐리온 도는 각각, .sigma. $_{s}$ =128 A $m^{2}$/kg $H_{A}$=6400 kA/m (80 kOe) 및 Tc=470 .deg. C로 질화처리 전에 비하여 크게 증가한다.크게 증가한다. 증가한다.

• Journal of applied mathematics & informatics
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• 제35권5_6호
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• pp.565-586
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• 2017

In this paper we continue to investigate the Super Vertex Mean behaviour of all graphs up to order 5 and all regular graphs up to order 7. Let G(V,E) be a graph with p vertices and q edges. Let f be an injection from E to the set {1,2,3,${\cdots}$,p+q} that induces for each vertex v the label defined by the rule $f^v(v)=Round\;\left({\frac{{\Sigma}_{e{\in}E_v}\;f(e)}{d(v)}}\right)$, where $E_v$ denotes the set of edges in G that are incident at the vertex v, such that the set of all edge labels and the induced vertex labels is {1,2,3,${\cdots}$,p+q}. Such an injective function f is called a super vertex mean labeling of a graph G and G is called a Super Vertex Mean Graph.

• 생명과학회지
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• 제14권5호
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• pp.732-740
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• 2004

MCM 단백질의 암세포내에서 과발현 되는 원인을 규명하기 위하여 mcm7, 2 promoter영역의 전사인자 결합 부위의 역할을 조사하였다. 암세포에서 promoter 활성은 정상세포보다 8배정도 높은 활성을 나타내 특유 전사인자의 존재를 시사하였다. Promoter영 역 에는 E2F 결합부위 와 Myc/Max/Mad단백질 결합 부위로 알려진 I-box가 존재한다. 이들 각각의 변이체 promoter를 작성하여 암세포와 정상세포에서 luciferase reporter 활성을 측정하는 것으로 각 영역의 역할을 검토하였다. 그 결과 정상세포 내에서는 강한 repressor존재 또는 활성을 negative로 조절 할 수 있는 complex의 존재로 promoter활성이 조절되는 반면 암세포 내에서는 I-box에 결합하여 promoter를 활성화시키는 특유 단백질의 존재가 시사되었다.

• 생명과학회지
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• 제7권4호
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• pp.358-360
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• 1997

대두 종자크기에 대한 QTL의 consistency을 알아보기 위하여 F$_{2}$, F$_{3}$ 세대에서의 분석결과를 요약하면 댜음과 같다. 세 개의 마크 OTL09a, OPM07a, OPAC12 가 F$_{2} 세대에서 종자 크기에 대한 QTL과 높은 유의성을 나타내었고, F$_{3}$ 세애데는 네 개의 마크 OTL09a, OPG19a, OPL09b, OPP11가 유의성을 나타내었다. 두 개의 마크 OPL09a, OPL09b 가 두 세대에서 유의성을 나타애어 종자 크기에 대한 QTL의 consistency가 인정되었다.

• 한국동물위생학회지
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• 제42권2호
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• pp.93-112
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• 2019

Calf diarrhea is a common disease in young claves and is still a major cause of productivity and economic loss in livestock farms. Fecal samples from Korean native cattle (n=100) with diarrhea from 64 farms in Gwangju area, Korea from september 2017 to December 2018 were examined for shedding of important protozoan parasitic, viral and bacterial pathogens using culture, rapid test kit and PCR methods. Of 57 (89.1%) of the 64 Korean native cattle farms examined had samples infected with at least one of the investigated pathogens. Among 100 fecal samples, 88 samples were positive for at least one the twelve pathogens and 51 samples were simultaneously positive for two or more pathogens by culture and PCR assay. Bovine group A rotavirus (BRV) was the most common pathogen, found in 43/100 (43.0%) samples on 32/64 (50.0%) farms. Subsequently, kobuvirus (30.0%), pathogenic E. coli (29.0%), bovine parvovirus (17.0%), Giardia spp. (13.0%), Eimeria spp. (10.0%), Clostridium perfringens type A (8.0%), bovine torovirus (8.0%), bovine viral diarrhea virus (6.0%), bovine coronavirus (5.0%), bovine norovirus (2.0%) and Cryptosporidium spp. (2.0%) were detected. Nebovirus, kırklareli virus, bovine adenovirus, Salmonella spp. and intestinal parasites were not detected. Of the 72 calves sampled in this age group, 64 (88.9%) samples were positive for at least one enteropathogen. BRV was identified in 34/72 (47.2%) samples from 27/48 (56.3%) farms. Subsequently, pathogenic E. coli (30.6%), kobuvirus (29.2%), BPaV (22.2%), Giardia spp. (15.3%), Eimeria spp. (9.7%), BVDV (6.9%), Cl. perfringens type A (6.9%), BCoV (4.6%) and Cryptosporidium spp. (2.8%) were detected in fecal samples. A total of ninety-six strains of E. coli were isolated from one hundred fecal samples collected from Korean native cattle with diarrhea. The presence of stx1, stx2, eaeA, LT, STa, STb, ehxA, saa, F4, F5(K99), F6, F17, F18 and F41 genes in the isolates was investigated by PCR. Out of ninety-six E. coli isolates screened for specific genes, 30 strains E. coli were identified to harbor shiga toxin-producing E. coli (STEC) 7 (7.3%), enterohemorrhagic E. coli (EHEC) 8 (8.3%), enteropathogenic E. coli (EPEC) 6 (6.3%), enterotoxigenic E. coli (ETEC) 2 (2.1%) and STEC/ETEC hybrid 7 (7.3%). This study provides epidemiological estimates of the prevalence of Korean native cattle's enteropathogens in Gwangju area, Korea, which would be used for cattle farmers and veterinarians to select appropriate therapeutic method.

• 대한음성학회지:말소리
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• 제63호
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• pp.47-66
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• 2007

This study aims at exploring the degree of phonetic similarity between Korean and Japanese vowels in terms of acoustic features by performing the speech production test on Korean speakers and Japanese speakers. For this purpose, the speech of 16 Japanese speakers for Japanese speech data, and the speech of 16 Korean speakers for Korean speech data were utilized. The findings in assessing the degree of the similarity of the 7 nearest equivalents of the Korean and Japanese vowels are as follows: First, Korean /i/ and /e/ turned out to display no significant differences in terms of F1 and F2 with their counterparts, Japanese /i/ and /e/, and the distribution of F1 and F2 of Korean /i/ and /e/ in the distributional map completely overlapped with Japanese /i/ and /e/. Accordingly, Korean /i/ and /e/ were believed to be "identical." Second, Korean /a/, /o/, and /i/ displayed a significant difference in either F1 or F2, but showed a great similarity in distribution of F1 and F2 with Japanese /a/, /o/, and /m/ respectively. Korean /a/ /o/, and /i/, therefore, were categorized as very similar to Japanese vowels. Third, Korean /u/, which has the counterpart /m/ in Japanese, showed a significant difference in both F1 and F2, and only half of the distribution overlapped. Thus, Korean /u/ was analyzed as being a moderately similar vowel to Japanese vowels. Fourth, Korean /${\wedge}$/ did not have a close counterpart in Japanese, and was classified as "the least similar vowel."