• Title/Summary/Keyword: E.coil

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Antibiotic Resistance of Pathogenic Escherichia coli Isolated from Piglets with Diarrhea (설사자돈 유래 대장균의 항생물질 내성에 관하여)

  • 조광현;박노찬;권헌일;김이준;박덕상
    • Korean Journal of Veterinary Service
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    • v.15 no.2
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    • pp.134-143
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    • 1992
  • The present study was conducted to investigate the biochemical characteristics and anti-biotic resistance of Escherichia coli(E. coli) isolated from piglets with diarrhea in Kyongbuk province during the Period from February to November 1991. 368 E. coli strains were isolated from 382 piglets with diarrhea and the biochemical and cultural reaction were compared with the classification criteria of Edwards and Ewing. Tetracycline and sulfadimethoxine were found to be highly ineffective at in vitro inhibition of the E. coli of piglets origin. The majority of E. coli were susceptible to amikacin, chloramphenicol and gentamicine. 89 (89.0%) of 100 strains of E. coil were resistant to one or more drugs. The organisms resistant to 20 or 3 drugs were 54(60.6%) of 89 strains, whereas 16(17.9%) strains were found to be resistant to one drug. 55(61.8%) out of 89 drug resistance strains carried R factors($R^+$) which were transfer-able to the recipients by conjugation.

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Feasibility & Limitations of Endovascular Coil Embolization of Anterior Communicating Artery Aneurysms

  • Hwang, Sung-Kyun;Benitez, Ronald;Veznedaroglu, Erol;Rosenwasser, Robert H.
    • Journal of Korean Neurosurgical Society
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    • v.38 no.2
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    • pp.89-95
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    • 2005
  • Objective : The purpose of this study is to analyze aneurysm morphology and define limitations and feasibility in endovascular Gugliemi detachable coil[GDC] embolization for anterior communicating artery [ACoA] aneurysms. Methods : From January 2000 through October 2003, 123patients were treated with endovascular coil embolization for ACoA aneurysms. There were 75women and 48men, with a mean age of 63years. All ruptured aneurysms were treated within 15days of rupture. Aneurysm morphology was classified according to neck size and projection of aneurysm dome as follows-A : neck of aneurysm <4mm & anterior projection, B : neck of aneurysm [4mm & anterior projection, C : neck of aneurysm<4mm & posterior [superior] projection, D : neck of aneurysm [4mm & posterior [superior] projection, E : neck of aneurysm<4mm & inferior projection, and F : neck of aneurysm [4mm & inferior projection. Endovascular procedures were categorized as either "successful" or "unsuccessful". Clinical follow-up was estimated at discharge and at 6months, post treatment results were classified according to Glasgow Outcome Scale[GOS]. Results : Successful embolization for ACoA was performed in 86patients of 123patients [69.9%]. Complete or near complete aneurysm occlusion was observed in 102patients [82.9%]; a neck remnant was observed in 6patients [4.9%]; partial embolization was done in 3patients [2.4%]; and embolization was attempted in 12patients [9.8%]. Among 55patients with follow-up angiographic results, 18patients [32.7%] were defined as recanalization of the aneurysm sac. Morphological analysis demonstrated that anterior projecting aneurysms and morphological classifications [morphological classifications worsens [A - D] chances of successful coil occlusion significantly decrease] were major factors in successful embolization, and, inferiorly projecting and wide neck [${\ge}4mm$] aneurysms are highly related to recanalization of aneurysms. Conclusion : Endovascular coil embolization of ACoA aneurysms shows good outcome in our study. Nevertheless, there is a limitation in the endovascular approach to ACoA, even though advanced modern techniques evolve rapidly. Compensatory surgical approach with the endovascular approach is required for successful treatment of ACoA aneurysms.

Wireless Energy and Data Transmission Using Inductive Coupling (유도결합방식에 의한 무선 에너지 및 데이터 전송)

  • Lee, Joon-Ha
    • Progress in Medical Physics
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    • v.19 no.1
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    • pp.42-48
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    • 2008
  • Bio-implantable devices such as heart pacers, gastric pacers and drug-delivery systems require power for carrying out their intended functions. These devices are usually powered through a battery implanted with the system or are wired to an external power source. This paper describes an inductive power transmission link, which was developed for an implantable stimulator for direct stimulation of denervated muscles. The carrier frequency is around 1MHz, the transmitter coil has a diameter of 46mm, and the implant coil is 46mm. Data transmission to the implant with amplitude shift keying (ASK) and back to the transmitter with passive telemetry can be added without major design changes. We chose the range of coil spacing (2 to 30mm) to care for lateral misalignment, as it occurs in practical use. If the transmitter coil has a well defined and reliable position in respect to the implant, a smaller working range might be sufficient. Under these conditions the link can be operated in fixed frequency mode, and reaches even higher efficiencies of up to 37%. The link transmits a current of 50 mA over a distance range of 2-15 mm with an efficiency of more than 20% in tracking frequency. The efficiency of the link was optimized with different approaches. A class E transmitter was used to minimize losses of the power stage. The geometry and material of the transmitter coil was optimized for maximum coupling. Phase lock techniques were used to achieve frequency tracking, keeping the transmitter optimally tuned at different coupling conditions caused by coil distance variations.

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Construction of a Corynebacteriurn glutarnicum-Escherichicr coli Shuttle Vector and Cloning the Homoserine ehydrogenase Gene from C. glutamicum (Corynebacterium glutamicum-Escherichia coli Shuttle Vector 개발과 C.glutamicum 의 Homoserine Dehydrogenase Gene Cloning)

  • 최신건;박종현;신현경
    • Microbiology and Biotechnology Letters
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    • v.19 no.1
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    • pp.31-36
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    • 1991
  • A 7.5 kilobases hybrid plasmid, designated as pCE1301, was constructed by combining Eschurichia cwli plasmid pBELl which carries the kanamycin resistance gene of Tn5 with a cryptic plasmid, pSRl of Corynebacterium glutamicum. pCE1301 was transformed C. glutaicum by PEG-mediated protoplast method and its transformation efficiency was about $3.0\times 10^3$ transformants per $\mu g$ of the hybrid plasmid DNA. The physical map reveals that pCE1301 has single restriction sites for SalI and EcoRl, respectively. 'The kanamycin resistance of pCE1301 was stably maintained in C. glutamicum up to 25 generations and any segregation was not detected. pCI31301 was also introduced into Brevibacterium flavum and E coil, and replicated in those strains. pCE1301 was proved to be useiul in cloning the homoscrine dehydrogenase gene from C. glutamicum.

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Fabrication of 250 m class Bi-2223/Ag HTS Tapes (250 m 급 Bi-2223/Ag 고온 초전도선재 제조)

  • Ha, H.S.;Oh, S.S.;Ha, D.W.;Jang, H.M.;Kim, S.C.;Song, K.J.;Park, C.;Kwon, Y.K.;Ryu, K.S.
    • Progress in Superconductivity
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    • v.3 no.1
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    • pp.130-133
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    • 2001
  • A multifilamentary Bi-2223 HTS tape for superconducting power applications was studied through the fabrication of 250-meter long tapes by the PIT(powder in tube) process. To fabricate continuous long wire, a drawing machine, a two-drum bull block and a rolled tape winding machine were developed. Especially, 250-meter long tapes were heat treated in the shape of pancake coil to reduce the heat affect zone and to achieve the high critical current. Engineering critical current density was improved through both the enhancements of critical current density by control of thermal process and the increase of filling factor by using thin Ag alloy sheath tubes less than 1.5 mm in thickness. We have made successfully 250-meter long 37 filamentary tapes with high filling factor up to 31 % employing the modified drawing and rolling technique. The critical current of 250-meter long tapes with pancake coil type was measured by transport method at self-field up to 250 gauss of center field. The measured values, based on the transport critical current at self-field, $I_{c}$ -B characteristics and magnetic field analysis, are 34 A of I$_{c}$ and 4.0 $kA/\textrm{cm}^2$ of $J_{e}$ at 250 m, 77 K, and 0 T. We also have achieved the 56 A of I$_{c}$ and 7.0 $0 kA/\textrm{cm}^2$ of$ J_{e}$ in short tapes at 77K, self-field, and 1$mutextrm{V}$/cm.

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Evaluation of the Noise Power Spectrum by Using American College of Radiology Phantom for Magnetic Resonance Imaging (자기공명영상에서 ACR 팬텀을 이용한 잡음전력스펙트럼 평가)

  • Jung-Whan Min;Hoi-Woun Jeong
    • Journal of radiological science and technology
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    • v.47 no.1
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    • pp.21-28
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    • 2024
  • This study was purpose to quantitative evaluation of comparison of the image intensity uniformity and noise power spectrum (NPS) by using American college of radiology (ACR) phantom for magnetic resonance imaging (MRI). The MRI was used achiva 3.0T MRI and discovery MR 750, 3.0T, the head and neck matrix shim SENSE head coil were 32 channels receive MR coil. The MRI was used parameters of image sequence for ACR standard and general hospital. NPS value of the ACR standard T2 vertical image in GE equipment was 7.65E-06 when the frequency was 1.0 mm-1. And the NPS value of the ACR hospital T1 region of interest (ROI) 9 over all vertical image in Philips equipment was 9E-08 when the frequency was 1.0 mm-1 and the NPS value of the hospital T2 ROI 9 over all vertical image in Philips equipment was 1.06E-07 when the frequency was 1.0 mm-1. NPS was used efficiently by using a general hospital vertical sequence more than the standard vertical sequence method by using the ACR phantom. Furthermore NPS was the quantitative quality assurance (QA) assessment method for noise and image intensity uniformity characteristics was applied mutatis mutandis, and the results values of the physical imaging NPS of the 3.0T MRI and ACR phantom were presented.

Antimicrobial Effects of Organic Acids and Ethanol on Several Foodborne Microorganisms (식중독 미생물에 대한 유기산 및 에탄올의 항균활성 비교연구)

  • Ahn, Yong-Seon;Shin, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.31 no.5
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    • pp.1315-1323
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    • 1999
  • The antimicrobial effects of ethanol and organic acids(acetic, citric, lactic. propionic, tartaric acid), either alone or in combination against four foodborne microorganisms (Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus and Escherichia coli O157:H7) in tryptic soy broth were determined. Area under the growth curve, minimum generation time, maximum growth rate, and detection time were measured by using automated turbidometer Bioscreen(Labsystem, Finland), for 24 hr at $30^{\circ}C$. All microorganisms were not grown at 7% ethanol in the media. The 0.1% propionic acid showed the strongest inhibitory effects against S. aureus, L. monocytogenes and E. coli O157 : H7 compared with other organic acids, whereas 0.01% organic acids did not show significant inhibitory effect against microorganisms tested (p > 0.01) except S. aureus. The combination of 1% ethanol and 0.01% organic acids were significantly more effective than alone on growth of S. aureus and L. monocytogenes(p < 0.01).

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Trichloro isocyanuric acid 와 Calcium hypochlorite에 의한 대장균 사멸특성 비교연구

  • Gang, Ja-Gyeong;Byeon, Dae-Jin;Kim, Eung-Su
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.430-433
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    • 2000
  • The disinfection effects of Trichloro isocyanuric acid (TICA) and Calcium hypochlorite (Cal-hypo) against E. coli' in aqueous suspension were compared at various concentrations of disinfectants as well as exposure times. When E, coil($^{\sim}10^7$ CFU/ml) were exposed by TICA and Cal-hypo (12 ppm each), 90% of the initial cells were reduced in 4 sec and 390 sec, respectively. Although Cal-hypo lost its disinfection capability in about 1 hr under the sun light, TICA maintained its effect up to 6 hrs. This comparative studies clearly demonstrate that TICA is more effective than Cal-hypo in terms of sterilizing E. coli as well as maintaining the disinfection effects.

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Probiotic Properties of Lactobacillus salivarius Isolated from Chicken Intestines (병아리의 장에서 분리한 Lactobacillus salivarius의 생균제로서 특성)

  • 박홍식;이지혜;엄태붕
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.5
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    • pp.1003-1009
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    • 1999
  • For the isolation of probiotics which may be used for the human beings and animals, we have screened the microorganisms from chicken intestines which have acid and bile tolerance and the growth inhibition of pathogenic E. coli and Salmonella typhimurium. Among them, a strain which was identified as Lactobacillus salivarius had around 66% of survival after 2h incubation in the artificial gastric juice and 9% of survival after 24h incubation in the presence of 0.3% bile salts, and showed complete inhibition against both path ogenic E. coli and Salmonella typhimurium after 24 h coincubation. Its storage stability after lyophilization could be improved by adding polyvinylpyrrolidone.

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$SiO_2$ Etching in $C_4F_{8}$ Plasma by E-ICP ($C_4F_{8}O_2$ 공정기체와 E-ICP를 이용한 산화막 식각)

  • 송호영;조수범;이종근;오범환;박세근
    • Proceedings of the IEEK Conference
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    • 2001.06b
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    • pp.197-200
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    • 2001
  • Novel Enhanced Inductively Coupled Plasma is applied to etch $SiO_2$. Effect of $O_2$ or Ar addition to $C_{4}F_{8}$ gas is monitored by Optical Emission Spectroscopy and Quadrupole Mass Spectrometer. It is fund that Ar or $O_2$ dilution to $C_{4}F_{8}$ increases F emission intensity and decreases $CF_2$ intensity. However, the ac frequency to the Helmholtz coil decreases the F intensity and thus increases $CF_2$/F ratio. By adjusting the ac frequency, the optimum etch rate and PR to $SiO_2$ selectivity can be obtained in E-lCP.

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