• Journal of Microbiology and Biotechnology
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• 제25권11호
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• pp.1810-1818
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• 2015

For the synthesis of various pharmaceuticals, chiral alcohols are useful intermediates. Among them, (R)-ethyl-4-chloro-3-hydroxybutanoate ((R)-ECHB) is an important building block for the synthesis of L-carnitine. (R)-ECHB is produced from ethyl-4-chloro-3-oxobutanoate (ECOB) by a reductase-mediated, enantioselective reduction reaction. The Saccharomyces cerevisiae YOL151W reductase that is expressed in Escherichia coli cells exhibited an enantioselective reduction reaction toward ECOB. By virtue of the C-terminal His-tag, the YOL151W reductase was purified from the cell-free extract using Ni²⁺-NTA column chromatography and immobilized onto Ni²⁺-magnetic microparticles. The physical properties of the immobilized reductase (Imm-Red) were measured using electron microscopy, a magnetic property measurement system, and a zeta potential system; the average size of the particles was approximately 1 μm and the saturated magnetic value was 31.76 emu/g. A neodymium magnet was used to recover the immobilized enzyme within 2 min. The Imm-Red showed an optimum temperature at 45℃ and an optimum pH at 6.0. In addition, Bacillus megaterium glucose dehydrogenase (GDH) was produced in the E. coli cells and was used in the coupling reaction to regenerate the NADPH cofactor. The reduction/oxidation coupling reaction composed of the Imm-Red and GDH converted 20 mM ECOB exclusively into (R)-ECHB with an e.e._p value of 98%.

• 한국ITS학회 논문지
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• 제10권2호
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• pp.103-111
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• 2011

최근 메쉬 네트워크는 다양한 분야에서 사용되고 있다. 그러나 메쉬 네트워크는 무선 전송의 특성으로 인한 제한적인 대역폭을 제공하며, 특히 단일 채널로 구성된 메쉬 네트워크의 경우 다중 홉 전송 시 전송률 저하 문제 등이 발생한다. 이러한 문제를 해결하고 QoS(Quality of Service)를 제공하기 위해 다양한 라우팅방식이 제안되어 왔다. 본 논문에서는 MPLS(Multi Protocol Label Switching)를 적용하여 QoS를 제공하는 MPLS 메쉬 네트워크 구조를 제안한다. MPLS를 적용한 메쉬 네트워크를 통해 하부 QoS 제공방식과 무관하게 상위 응용계층에서의 경로관리가 용이해지며 트래픽관리가 용이해진다. 본 논문에서는 IEEE 802.11e 표준을 적용하여 트래픽에 대한 차별적인 서비스를 제공하도록 MPLS 적용 메쉬 라우터를 설계하고, 구현 및 실험을 통하여 동작을 검증하였다.

• Nuclear Engineering and Technology
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• 제43권3호
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• pp.279-286
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• 2011

The aging degradation and lifetime assessment of a domestic class 1E Ethylene-Propylene-Diene-Monomer (EPDM), which is a popular insulating elastomer for electrical cables in the nuclear power plants, were studied for equipment qualification verification under the Loss of Coolant Accident (LOCA) conditions. The specimens were acceleratively aged, underwent a LOCA environment, as well as tested mechanically, thermo-gravimetrically, and spectroscopically according to the American Society of the Testing of Materials (ASTM) procedures. The tensile test results revealed that the elongation at break gradually decreased with an increasing aging temperature. The lifetime of EPDM aged isothermally at $140^{\circ}C$ was 1,316 hours and reduced to 1,120 hours after experiencing the severe accident test. The activation energies of the elongation reduction were $1.10{\pm}0.196$ eV and $0.93{\pm}0.191$ eV before and after the LOCA condition, respectively. The TGA test results also showed that the activation energy of the aging decomposition decreased from 1.35 eV to 1.02 eV after undergoing the LOCA environment. Although the mechanical property changes were discernibly observed during the aging process, along with the LOCA simulation, the FT-IR analysis showed that the spectroscopic peaks and their intensities did not alter significantly. Therefore, it can be concluded that the degradation of the domestic class 1E EPDM due to aging can be tolerable, even in severe accident conditions such as LOCA, and thus it qualifies as a suitable insulating material for electrical cables in the nuclear power plants.

• Corrosion Science and Technology
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• 제9권6호
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• pp.310-316
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• 2010

Recently a fuel oil of the diesel engine of the marine ship is being changed with heavy oil of low quality as the oil price is higher more and more. Therefore the wear and corrosion in all parts of the engine such as cylinder liner, piston crown, spindle and seat ring of exhaust valves are predominantly increased. In particular the degree of wear and corrosion of piston crown is more seriously compared to the other parts of the engine due to operating in severe environment such as the high temperature of exhaust gas and repeating impact. Thus the repair weldment of the piston crown is a unique method to prolong the its life in a economical point of view. In this case, filler metals having a high corrosion and wear resistance such as stellite 6, Inconel 625 and Inconel 718 are mainly being used for repair welding. However it has been often happened that piston crown on the ship,s job site is being actually inevitably welded with mild filler metals. Therefore in this study, filler metals such as E4301, E4313 and E4316 were welded at SS401 steel as the base metal, and corrosion property of their weld metals in the case of post weld heat treatment or not was investigated with some electrochemical methods such as measurement of corrosion potential, cathodic and anodic polarization curves, cyclic voltammogram and polarization resistance etc. in 0.1% $H_2SO_4$ solution. Corrosion resistance of the weld metal of E4301 was better than the other weld metals in the case of no heat treatment, however, its resistance was considerably decreased with post weld heat treatment(annealing:$625^{\circ}C$, 2 hr) compared to other weld metals. The weld metals of E4313 and E4316 showed a relatively good corrosion resistance by post weld heat treatment.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제12권5호
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• pp.2029-2043
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• 2018

In this paper, we propose a novel inter-cell handover approach from a new perspective in dense Heterogeneous and Small Cell Networks (HetSNets). We first devise a cell selection mechanism to choose a proper candidate small cell for the UEs that tend to implement inter-small cell handover (ICH). By exploiting the property of a typical non-concentric circle, i.e., circle of Apollonius, we then propose a novel analytical method for modeling inter-cell handover regions and present mathematical derivation to prove that the inter-small cell handover issues fit the property of the circle of Apollonius. We design an inter-cell handover algorithm (ICHA) by means of our proposed handover model to dynamically configure hysteresis margin and properly implement handover decision in terms of UE's mobility. Simulation results demonstrate that the proposed ICHA yields lower call drop rate and radio link failure rate than the conventional methods and hence achieve high Handover Performance Indicator (HPI).

• 한국정보과학회논문지:데이타베이스
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• 제31권4호
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• pp.395-408
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• 2004

논리곱 불리언 질의는 질의에 포함된 키워드들이 모두 나타나는 텍스트 문서들을 검색하는질의로서, 정보검색 시스템에서 가장 널리 사용되는 질의이다. 논리곱 불리언 질의는 검색의 정확도를 높이기 위하여 많은 수의 키워드로 구성된 긴 질의를 사용한다. 이 경우. 키워드 처리 순서가 성능에 크게 영향을 미친다. 기존 정보검색시스템에서는 휴리스틱에 의존하여 키워드 처리 순서를 결정하므로 최적을 보장하지 못한다. 동적 프로그래밍과 같은 기존의 데이타베이스 질의 최적화 알고리즘은 복잡도가 지수적으로 증가하므로(Ο(n2$^{n-1}$)), 키워드 수가 많은 논리곱 불리언 질의에는 적합하지 않다. 본 논문에서는 조인시퀀스 분리성이라는 새로운 개념에 기반한 논리곱 불리언 질의 최적화 알고리즘을 제안한다. 조인 시퀀스 분리성이란 조인에 참여하는 릴레이션들이 어떤 조건을 만족할 경우, 최적 조인 시퀀스가 두 개의 서브 시퀀스로 분리된다는 성질이다. 이 성질을 활용하면 Ο(nlogn)만에 최적 조인 시퀀스를 구할 수 있다. 본 논문에서는 이러한 조인 시퀀스 분리성의 개념을 정형적으로 정의하고 이에 기반한 질의 최적화 알고리즘의 최적성을 이론적으로 증명한다. 그리고, 제안한 질의 최적화 알고리즘의 성능 평가를 위해, 비용 모델을 사용하여 다양한 시뮬레이션을 수행한다. 그 결과, 제안한 알고리즘의 성능이 기존의 휴리스틱 기반 질의 최적화 알고리즘들에 비해 100배 이상 우수함을 보인다. 또한, 동적 프로그래밍 알고리즘에 비해 질의 최적화 시간 면에서 기하 급수적으로 우수함을 보인다(키워드 개수가 10 개일 경우 600배 이상 우수함).

• 대한기계학회논문집
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• 제19권8호
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• pp.1876-1888
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• 1995

In this paper, glass surface-mat reinforced epoxy(G.S.R.E.) is developed, It is assured that the material(G.S.R.E.) can be used as photoelastic model material and it satisfy with the required properties of photoelastic model material. Therefore, the material can be used as model material of transparent photoelastic experiment when we analyze the stress distributions of transversely isotropic material by photoelastic experiment. When we use G.S.R.E. as photoelastic experiment model material, we had better use the G.S.R.E. which fiber volume ratio is less than 0.7% in the high temperature(stress freezing method) and than 1.74% in the room temperature. Relationships between stress fringe value and elastic modulus in transversely isotropic material are developed in this paper, it is assured by experiment that they are established in the room temperature or in the high temperature. Therefore we can obtain stress fringe value or elastic modulus from the relationships between stress fringe value and elastic modulus.

• 대한수의학회지
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• 제34권4호
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• pp.759-768
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• 1994

E coli strains isolated from pigs were investigated with respect to antimicrobial resistances and prevalence of R-plasmids. Also determined were properties of R-plasmids by plasmid conjugation, curing and southern hybridization using gene probes. All of 400 E coli strains were resistant to CL and SU, and 0.3% to 96.8% of the strains were resistant to most antimicrobials such as TC, PG, AM, SM, CP, GM, EM, NM, etc, while all strains were sensitive to AK. All strains were also multiply resistant to three to twelve antimicrobials. The resistances to PG, SM, TC, AM, CP, SU and ST were transferable and supposed to be mediated by R-plasmids which were opportunistic for transposition into chromosome. Plasmids bigger in size than chromosomal DNA were considered as R-plasmids and most plasmids in small size (<4Kb) proved as cryptic plasmids or nonconjugative R-plasmids. In a strain(No 99), AM resistant property was determined from both chromosomal DNA and R-plasmid DNA which is bigger in size than chromosome.

• 대한기계학회논문집A
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• 제34권7호
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• pp.821-827
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• 2010

본 논문은 밀도법을 기반으로 한 자기포화 효과를 고려한 수직자기 기록장치의 위상최적 설계 방법을 제안한다. 자기장 내의 위상최적화 과정에서 자기 저항률의 값은 민감도 해석 결과에 따른 요소의 밀도값 변화에 의거하여 결정된다. B-H 곡선에 따른 자기 저항률의 값은 물성치의 비선형성을 나타낸다. 일반화된 응답 함수의 민감도는 자기장의 비선형 특성을 고려하여 보조 변수법에 의해 표현되고, 설계의 목적 함수는 기록 영역의 자기에너지를 최대화하는 것으로 설정된다. 선형과 비선형의 최적화의 수치 연구의 결과는 비선형의 효과를 나타내고 있다.

• 대한기계학회논문집A
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• 제32권2호
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• pp.105-118
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• 2008

The newly developed analysis method for nanoindentation load-displacement curves are focused on not only obtaining elastic modulus and hardness values but also other mechanical properties, such as yield strength and strain hardening properties. Dao et al. developed a forward and reverse algorithm to extract the elasto-plastic properties of materials from the load-displacement curves obtained in nanoindentation test. These algorithms were only applicable for engineering metals (Poisson#s ratio 0.3) using the equivalent conical indenter of the Berkovich. However, the applicable metals are substantially limited because range of used in the finite element analysis is narrow. This study is designed to expand range of the applicable metals in the reverse algorithms established by Dao et al. and to improve the accuracy of that for extracting the elasto-plastic properties of materials. In this study, a representative strain was assumed to vary according to specific range of $E^*/{\sigma}_r$ and was defined as function of $E^*/{\sigma}_r$. Also, an initial unloading slope in reverse algorithms improved in this study was not considered as independent parameters of the load-displacement curves. The mechanical properties of materials for finite element analysis were modeled with the elastic modulus, E, the yield strength, ${\sigma}_y$, and the strain hardening exponents, n. We showed that the representative strain (0.033) suggested by Dao et al. was no longer applicable above the $E^*/{\sigma}_r$ of 400 and depended on values of $E^*/{\sigma}_r$. From these results, we constructed the dimensionless functions, in where the initial unloading slope was not included, for engineering metals up to $E^*/{\sigma}_r$ of 1500. These functions allow us to determine the mechanical properties with greater accuracy than Dao#s study.