• Journal of Microbiology and Biotechnology
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• v.25 no.11
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• pp.1810-1818
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• 2015

For the synthesis of various pharmaceuticals, chiral alcohols are useful intermediates. Among them, (R)-ethyl-4-chloro-3-hydroxybutanoate ((R)-ECHB) is an important building block for the synthesis of L-carnitine. (R)-ECHB is produced from ethyl-4-chloro-3-oxobutanoate (ECOB) by a reductase-mediated, enantioselective reduction reaction. The Saccharomyces cerevisiae YOL151W reductase that is expressed in Escherichia coli cells exhibited an enantioselective reduction reaction toward ECOB. By virtue of the C-terminal His-tag, the YOL151W reductase was purified from the cell-free extract using Ni²⁺-NTA column chromatography and immobilized onto Ni²⁺-magnetic microparticles. The physical properties of the immobilized reductase (Imm-Red) were measured using electron microscopy, a magnetic property measurement system, and a zeta potential system; the average size of the particles was approximately 1 μm and the saturated magnetic value was 31.76 emu/g. A neodymium magnet was used to recover the immobilized enzyme within 2 min. The Imm-Red showed an optimum temperature at 45℃ and an optimum pH at 6.0. In addition, Bacillus megaterium glucose dehydrogenase (GDH) was produced in the E. coli cells and was used in the coupling reaction to regenerate the NADPH cofactor. The reduction/oxidation coupling reaction composed of the Imm-Red and GDH converted 20 mM ECOB exclusively into (R)-ECHB with an e.e._p value of 98%.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.10 no.2
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• pp.103-111
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• 2011

Recently, wireless mesh networks are used in various application areas. However, wireless mesh networks have limited bandwidth by the wireless transmission property, and have severe throughput degradation in multi-hop transmission in single channel wireless mesh networks. To solve this problem and support QoS, a lot of routing protocols have been proposed in mesh networks. In this paper, we propose a wireless mesh networks architecture with MPLS for QoS service. The path and traffic management from the application could be independent from QoS routing protocols by using the MPLS in wirelss mesh networks. In this paper, we design a MPLS-based mesh router with IEEE 802.11e for traffic differentiation and investigate the operation by implementation and test.

• Nuclear Engineering and Technology
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• v.43 no.3
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• pp.279-286
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• 2011

The aging degradation and lifetime assessment of a domestic class 1E Ethylene-Propylene-Diene-Monomer (EPDM), which is a popular insulating elastomer for electrical cables in the nuclear power plants, were studied for equipment qualification verification under the Loss of Coolant Accident (LOCA) conditions. The specimens were acceleratively aged, underwent a LOCA environment, as well as tested mechanically, thermo-gravimetrically, and spectroscopically according to the American Society of the Testing of Materials (ASTM) procedures. The tensile test results revealed that the elongation at break gradually decreased with an increasing aging temperature. The lifetime of EPDM aged isothermally at $140^{\circ}C$ was 1,316 hours and reduced to 1,120 hours after experiencing the severe accident test. The activation energies of the elongation reduction were $1.10{\pm}0.196$ eV and $0.93{\pm}0.191$ eV before and after the LOCA condition, respectively. The TGA test results also showed that the activation energy of the aging decomposition decreased from 1.35 eV to 1.02 eV after undergoing the LOCA environment. Although the mechanical property changes were discernibly observed during the aging process, along with the LOCA simulation, the FT-IR analysis showed that the spectroscopic peaks and their intensities did not alter significantly. Therefore, it can be concluded that the degradation of the domestic class 1E EPDM due to aging can be tolerable, even in severe accident conditions such as LOCA, and thus it qualifies as a suitable insulating material for electrical cables in the nuclear power plants.

• Corrosion Science and Technology
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• v.9 no.6
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• pp.310-316
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• 2010

Recently a fuel oil of the diesel engine of the marine ship is being changed with heavy oil of low quality as the oil price is higher more and more. Therefore the wear and corrosion in all parts of the engine such as cylinder liner, piston crown, spindle and seat ring of exhaust valves are predominantly increased. In particular the degree of wear and corrosion of piston crown is more seriously compared to the other parts of the engine due to operating in severe environment such as the high temperature of exhaust gas and repeating impact. Thus the repair weldment of the piston crown is a unique method to prolong the its life in a economical point of view. In this case, filler metals having a high corrosion and wear resistance such as stellite 6, Inconel 625 and Inconel 718 are mainly being used for repair welding. However it has been often happened that piston crown on the ship,s job site is being actually inevitably welded with mild filler metals. Therefore in this study, filler metals such as E4301, E4313 and E4316 were welded at SS401 steel as the base metal, and corrosion property of their weld metals in the case of post weld heat treatment or not was investigated with some electrochemical methods such as measurement of corrosion potential, cathodic and anodic polarization curves, cyclic voltammogram and polarization resistance etc. in 0.1% $H_2SO_4$ solution. Corrosion resistance of the weld metal of E4301 was better than the other weld metals in the case of no heat treatment, however, its resistance was considerably decreased with post weld heat treatment(annealing:$625^{\circ}C$, 2 hr) compared to other weld metals. The weld metals of E4313 and E4316 showed a relatively good corrosion resistance by post weld heat treatment.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.5
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• pp.2029-2043
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• 2018

In this paper, we propose a novel inter-cell handover approach from a new perspective in dense Heterogeneous and Small Cell Networks (HetSNets). We first devise a cell selection mechanism to choose a proper candidate small cell for the UEs that tend to implement inter-small cell handover (ICH). By exploiting the property of a typical non-concentric circle, i.e., circle of Apollonius, we then propose a novel analytical method for modeling inter-cell handover regions and present mathematical derivation to prove that the inter-small cell handover issues fit the property of the circle of Apollonius. We design an inter-cell handover algorithm (ICHA) by means of our proposed handover model to dynamically configure hysteresis margin and properly implement handover decision in terms of UE's mobility. Simulation results demonstrate that the proposed ICHA yields lower call drop rate and radio link failure rate than the conventional methods and hence achieve high Handover Performance Indicator (HPI).

• Journal of KIISE:Databases
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• v.31 no.4
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• pp.395-408
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• 2004

A conjunctive Boolean text query refers to a query that searches for tort documents containing all of the specified keywords, and is the most frequently used query form in information retrieval systems. Typically, the query specifies a long list of keywords for better precision, and in this case, the order of keyword processing has a significant impact on the query speed. Currently known approaches to this ordering are based on heuristics and, therefore, cannot guarantee an optimal ordering. We can use a systematic approach by leveraging a database query processing algorithm like the dynamic programming, but it is not suitable for a text query with a typically long list of keywords because of the algorithm's exponential run-time (Ο(n2$^{n-1}$)) for n keywords. Considering these problems, we propose a new approach based on a property called the join sequence separability. This property states that the optimal join sequence is separable into two subsequences of different join methods under a certain condition on the joined relations, and this property enables us to find a globally optimal join sequence in Ο(n2$^{n-1}$). In this paper we describe the property formally, present an optimization algorithm based on the property, prove that the algorithm finds an optimal join sequence, and validate our approach through simulation using an analytic cost model. Comparison with the heuristic text query optimization approaches shows a maximum of 100 times faster query processing, and comparison with the dynamic programming approach shows exponentially faster query optimization (e.g., 600 times for a 10-keyword query).

• Transactions of the Korean Society of Mechanical Engineers
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• v.19 no.8
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• pp.1876-1888
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• 1995

In this paper, glass surface-mat reinforced epoxy(G.S.R.E.) is developed, It is assured that the material(G.S.R.E.) can be used as photoelastic model material and it satisfy with the required properties of photoelastic model material. Therefore, the material can be used as model material of transparent photoelastic experiment when we analyze the stress distributions of transversely isotropic material by photoelastic experiment. When we use G.S.R.E. as photoelastic experiment model material, we had better use the G.S.R.E. which fiber volume ratio is less than 0.7% in the high temperature(stress freezing method) and than 1.74% in the room temperature. Relationships between stress fringe value and elastic modulus in transversely isotropic material are developed in this paper, it is assured by experiment that they are established in the room temperature or in the high temperature. Therefore we can obtain stress fringe value or elastic modulus from the relationships between stress fringe value and elastic modulus.

• Korean Journal of Veterinary Research
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• v.34 no.4
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• pp.759-768
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• 1994

E coli strains isolated from pigs were investigated with respect to antimicrobial resistances and prevalence of R-plasmids. Also determined were properties of R-plasmids by plasmid conjugation, curing and southern hybridization using gene probes. All of 400 E coli strains were resistant to CL and SU, and 0.3% to 96.8% of the strains were resistant to most antimicrobials such as TC, PG, AM, SM, CP, GM, EM, NM, etc, while all strains were sensitive to AK. All strains were also multiply resistant to three to twelve antimicrobials. The resistances to PG, SM, TC, AM, CP, SU and ST were transferable and supposed to be mediated by R-plasmids which were opportunistic for transposition into chromosome. Plasmids bigger in size than chromosomal DNA were considered as R-plasmids and most plasmids in small size (<4Kb) proved as cryptic plasmids or nonconjugative R-plasmids. In a strain(No 99), AM resistant property was determined from both chromosomal DNA and R-plasmid DNA which is bigger in size than chromosome.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.7
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• pp.821-827
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• 2010

This paper proposes a density method based topology optimization of a perpendicular magnetic recording system design in which the saturation effect is taken into account. During the topology optimization process in magnetic fields, the magnetic reluctivity is updated in accordance with the changes in element density determined by a sensitivity analysis. The magnetic reluctivity is determined from a B-H curve and is used to represent nonlinear material property, i.e., the saturation effect. The sensitivity for a generalized response functional is formulated using the adjoint variable method in which the nonlinear property is taken into account and the objective function is set such that the magnetic energy in the media is maximized. Effects due to the nonlinear property can be observed from a numerical study in which the linear and the nonlinear topology optimization results are compared.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.32 no.2
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• pp.105-118
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• 2008

The newly developed analysis method for nanoindentation load-displacement curves are focused on not only obtaining elastic modulus and hardness values but also other mechanical properties, such as yield strength and strain hardening properties. Dao et al. developed a forward and reverse algorithm to extract the elasto-plastic properties of materials from the load-displacement curves obtained in nanoindentation test. These algorithms were only applicable for engineering metals (Poisson#s ratio 0.3) using the equivalent conical indenter of the Berkovich. However, the applicable metals are substantially limited because range of used in the finite element analysis is narrow. This study is designed to expand range of the applicable metals in the reverse algorithms established by Dao et al. and to improve the accuracy of that for extracting the elasto-plastic properties of materials. In this study, a representative strain was assumed to vary according to specific range of $E^*/{\sigma}_r$ and was defined as function of $E^*/{\sigma}_r$. Also, an initial unloading slope in reverse algorithms improved in this study was not considered as independent parameters of the load-displacement curves. The mechanical properties of materials for finite element analysis were modeled with the elastic modulus, E, the yield strength, ${\sigma}_y$, and the strain hardening exponents, n. We showed that the representative strain (0.033) suggested by Dao et al. was no longer applicable above the $E^*/{\sigma}_r$ of 400 and depended on values of $E^*/{\sigma}_r$. From these results, we constructed the dimensionless functions, in where the initial unloading slope was not included, for engineering metals up to $E^*/{\sigma}_r$ of 1500. These functions allow us to determine the mechanical properties with greater accuracy than Dao#s study.