• Korean Journal of Microbiology
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• v.32 no.3
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• pp.198-201
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• 1994

The nucleotide sequence of the cohesive end site (cos) of Lactobacillus casei phage J1 genome was determined. Comparison between the nucleotide sequences of the circular cos and the left end of the linear J1 DNA showed that the nicking sites of the terminase were as follows: 5'- GGTCGGCC$\downarrow$ -3' 3'- $\uparrow$CCAGCCGG -5' The cohesive single-stranded ends of J1 were found to be 3'-protruding and composed of 8 nucleotides. The mol% G + C of the cohesive ends was 87.5. The cos site shows dyad symmetry from -33 to + 25 bp if the 5' terminal nucleotide of the left end of the linear J1 DNA is numbered +1. No homology was found among the cos sites of phages reported so far.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.6
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• pp.256-262
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• 2017

This study examined the peer and gender effects in knowledge-sharing behavior. More specifically, this paper examined how coworker social loafing is related to knowledge sharing and how gender differences moderate the relationship between coworker social loafing and knowledge-sharing behavior. Drawing on economic and social exchange theory, this study predicts that coworker social loafing will decrease the knowledge-sharing behavior. In addition, this paper hypothesized that men will be more likely to withdraw knowledge-sharing behavior than women when they faced coworker social loafing. To test these hypotheses, this paper conducted a hierarchical regression test with the supervisor-employee dyad samples. The empirical results showed that in the relationship between coworker social loafing and knowledge-sharing behavior, coworker social loafing decreased the knowledge-sharing behavior, and the negative effect was larger in the case of men rather than women. In the discussion section, this paper proposes the theoretical and practical implications based on theoretical arguments and empirical findings.

• Korean Journal of Child Studies
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• v.8 no.1
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• pp.1-14
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• 1987

The purpose of the present research was to study the self-regulation of the child during mother child interaction. That is. this study focused on how mothers regulated children's problem solving efforts before children began to function as independent agents capable of self-regulation. The emphasis was on the "regulation-by-others" that preceded "self-regulation". The subjects of this study were 30 mother-child dyads. The dyads were divided into 3 groups of 10 dyads each on the basis of the child's age (2, 3, and 4 years of age). The instrument used for this study was the "truck puzzle" devised by Wertsch, McNamee, Mclane and Budwig (1980). The problem solving task in this study required the dyad to make a puzzle in accordance with the model puzzle. The interaction of 30 mother-child dyads was observed and analyzed flH verbal and non-verbal behavior used by the mother to regulate the child's behavior. One-way ANOVA, Scheffe post hoc comparision and Spearman's rank-difference correlation were used for the statistical analysis of the data. The results revealed that there were significant age trends in regulation-by-others and self regulation in joint cognitive activity; that is, there were developmental transitions from regulation by-others to self-regulation in connection with crucial strategic behavior (looking at the model). Significant differences were found in mothers' interventions after children's gaze at the model in joint cognitive activity; that is, the mean proportion of mothers' intervention for the 2-year-old group was the highest and that of the 4-year-old group was the lowest. There were no significant age-related differences in mothers' use of referential perspectives in joint cognitive activity. Children's regulation- by-others increased and children's self-regulation decreased in proportion to mothers' use of referential perspectives.

• Applied Microscopy
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• v.15 no.1
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• pp.71-85
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• 1985

The ultrastructure on the dorsal vessel of 5-day-old cabbage butterfly, Pieris rapae L., was carried out using the transmission and scanning electron microscope. The results are as follows. 1) The aorta. The aorta is simple tubular type and consists of the inner and outer membrane of the myocardium and thick myocardium is located between them. However the inner membrane with $0.26{\mu}m$ thickness and outer membrane with $0.08{\mu}m$ are composed of fibrous materials, the former is composed of low and high densed fibrous materials and the latter appears homogeneous layer. The myocardium consists of typical striated muscles. The sarcomere with $1.6{\mu}m$ length and in cross section, each thick filaments are surrounded by $7{\sim}8$ thin filaments. The intercalated disc is joining the end of the two muscle cells, desmosomes and septate junctions are appeared between the neighboring muscle cells. 2) The heart. The heart composing of myocardium enclosed by its inner and outer membrane as the aorta has a series of well formed segmental chamber. The arrangement of myofilaments, cell adhensions and membrane elements are observed as same as at the aorta. The inner membrane of the heart is deeply invaginated into the myocardium than the outer membrane and a lot of well developed mitochondria with rod shape are aggregated in the folds. The longitudinally and transversely oriented tubule system formed by invagnation of the sarcolemma into the muscle bundle is built up dyad with the sarcoplasmic reticulum as the aorta. The slit is formed by deeply invagination of the inner membrane of myocadium toward the muscle layer and then the inner and outer membrane of myocardium are fused. Therefore, the ostium is formed between the myocardium and situated at the lateral side of the myocardium.

• Proceedings of the Botanical Society of Korea Conference
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• 1987.07a
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• pp.149-155
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• 1987

Growth and development of a higher plant, or any living organism for that matter, could be defined as an orderly expression of the genome in time and space in close interaction with the environment. During differentiation and development of a tissue or organ a group of genes must be selectively turned on or turned off mainly by trans-acting regulators. In this general concept of regulation of regulation of gene expression, a DNA molecule is recognized at a specific nucleotide sequence by DNA-binding factors. Molecular biology of the regulatory factors such as hormones, and their receptors, target DNA sequences and DNA-binding proteins are well advanced. What is not clearly understood is the molecular basis of the interactions between DNA and binding factors, expecially of the usages of the dyad symmetry of the target DNA sequences and the dimeric nature of the DNA-binding proteins. A unique 3-dimensional structure of DNA has been proposed that may play an important role in the orderly expression of the gene. A foldback intercoil (FBI) DNA configuration which was originally found by electron microscopy among mtDNA molecules from pearl millet has some unique features. The FBI configuration of DNA is believed to be formed when a flexible double helix folds back and interwines in the widened major grooves resulting in a four stranded, intercoil DNA whose thickness is the same as that of double stranded DNA. More recently, the FBI structure of DNA has been also induced in vitro by a novel enzyme which was purified from pearl millet mitochondria. It has been proposed that the FBI DNA could be utillized in intramolecular recombination which leads to inversion or deletion, and in intermolecular recombination which can lead to either site-specific recombination, genetic recombination via single strand invasion, or cross strand recombination. The structure and function of DNA in 3-dimensional aspect is emphasized for better understanding orderly expression of genes during growth and development.

• Korean Journal of Childcare and Education
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• v.10 no.6
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• pp.43-56
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• 2014

The purpose of this study was to analyze the differences of preschoolers' utterance features by their gender and play situation type. For this purpose, a total of 40 5-year-old children participated in this study. Dyad were participated in each play session during 10 minutes. The play session was videotaped and the videotaped data were transcribed by CBS(2014). The collected data were analyzed by using a independent t-test and paired t-test. The main results are as follows. First, girls' MLU-e, MLU-w, MLU-m were longer than that of boys in a familiar play situation. Second, preschoolers' MLU-w was longer in an unfamiliar play situation than in familiar ones and preschoolers' type-token ratio were higher in an unfamiliar play situation than in familiar ones. Implications for the importance of preschoolers' spontaneous speech are discussed.

• Molecules and Cells
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• v.21 no.1
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• pp.129-134
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• 2006

Lon, also known as protease La, belongs to a class of ATP-dependent serine protease. It plays an essential role in degradation of abnormal proteins and of certain short-lived regulatory proteins, and is thought to possess a Ser-Lys catalytic dyad. To examine the structural organization of Lon, we performed an electron microscope analysis. The averaged images of Lon with end-on orientation revealed a six-membered, ring-shaped structure with a central cavity. The side-on view showed a two-layered structure with an equal distribution of mass across the equatorial plane of the complex. Since a Lon subunit possesses two large regions containing nucleotide binding and proteolytic domains, each layer of the Lon hexamer appears to consist of the side projections of one of the major domains arranged in a ring. Lon showed a strong tendency to form hexamers in the presence of $Mg^{2+}$, but dissociated into monomers and/or dimers in its absence. Moreover, $Mg^{2+}$-dependent hexamer formation was independent of ATP. These results indicate that Lon has a hexameric ring-shaped structure with a central cavity, and that the establishment of this configuration requires $Mg^{2+}$, but not ATP.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.2
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• pp.625-631
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• 2021

This study examined the effects of self-critical perfectionism on organizational citizenship behavior (OCB) and the moderating effects of gender in the relationship to understand deeply the dilemmatic situation resulting from OCB at the individual level. In other words, based on the conservation of resources theory, this study examined how self-critical perfectionism influences the OCB and how gender moderates the relationship between self-critical perfectionism and OCB. More specifically, this study predicts that self-perfectionism will be negatively related to OCB. Furthermore, this negative relationship between self-critical perfectionism and OCB will be strengthened in men compared to women. To test these hypotheses, the supervisor-subordinate survey was conducted, and 150 dyad data were analyzed. The analytical results of HLM (Hierarchical Linear Modeling) showed that self-critical perfectionism negatively influenced the OCB, and this negative effect was strengthened in men compared to women. This paper suggests implications and limitations for the literature on OCB.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.25 no.3
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• pp.194-210
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• 2022

Human milk contains a number of nutritional and bioactive molecules including microorganisms that constitute the so-called "Human Milk Microbiota (HMM)". Recent studies have shown that not only bacterial but also viral, fungal, and archaeal components are present in the HMM. Previous research has established, a "core" microbiome, consisting of Firmicutes (i.e., Streptococcus, Staphylococcus), Proteobacteria (i.e., Serratia, Pseudomonas, Ralstonia, Sphingomonas, Bradyrhizobium), and Actinobacteria (i.e., Propionibacterium, Corynebacterium). This review aims to summarize the main characteristics of HMM and the role it plays in shaping a child's health. We reviewed the most recent literature on the topic (2019-2021), using the PubMed database. The main sources of HMM origin were identified as the retrograde flow and the entero-mammary pathway. Several factors can influence its composition, such as maternal body mass index and diet, use of antibiotics, time and type of delivery, and mode of breastfeeding. The COVID-19 pandemic, by altering the mother-infant dyad and modifying many of our previous habits, has emerged as a new risk factor for the modification of HMM. HMM is an important contributor to gastrointestinal colonization in children and therefore, it is fundamental to avoid any form of perturbation in the HMM that can alter the microbial equilibrium, especially in the first 100 days of life. Microbial dysbiosis can be a trigger point for the development of necrotizing enterocolitis, especially in preterm infants, and for onset of chronic diseases, such as asthma and obesity, later in life.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.26 no.2
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• pp.88-98
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• 2023

Purpose: Children with inflammatory bowel disease (IBD) frequently undergo clinical assessments, involving triadic communication between clinician, parent, and child. During such encounters parents are traditionally the main communicator of information on their child's IBD, including subjective symptom reports. The level of agreement between children and their parents for IBD symptoms is poorly understood, and this study aimed to examine this factor. Methods: This was a cross-sectional study among children with IBD, and one parent. A validated paediatric IBD symptom report tool (IBDnow) enabled children and their parent to rate seven pain, well-being, and stool metrics, with dyads completing the tool concurrently. Results were assessed using: Individual agreement: proportion of identical symptom reports by each dyad (ideal score >0.7); Category agreement: percentage of identical reports for IBDnow metrics for the cohort; Inter-rater reliability: Gwet's AC1 coefficient with higher scores indicating better reliability (maximum=1). Results: Seventy-four parent/child dyads participated; child's mean age 12.2 years (standard deviation [SD] 2.9, range 6-16), mean time since diagnosis 2.8 years (SD 3), 54% female, 73% had Crohn's Disease. Mean individual agreement level was 0.6, with 27% of dyads agreeing on ≥6/7 IBDnow metrics. Category agreement was reported by 61% of dyads, 20% of parents overestimated, and 19% underestimated, their child's symptoms. Inter-rater reliability ranged from fair to good. Conclusion: These results should improve clinician awareness of how IBD symptom reports from parents may introduce bias. Children should be considered the most important source of symptom reports, and tools such as IBDnow utilised to enhance communication.